FastQCFastQC Report
Thu 26 May 2016
SRR937297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485409
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33360.6872554897004381No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28990.5972283167390798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18830.38792028989985766No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.1522427478682925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19400.044.58541
GTATCAA31850.036.5577661
TATCAAC40350.028.9624672
ATCAACG40550.028.5853143
TCAACGC41350.028.0322724
CAACGCA41950.027.6313365
AACGCAG42500.027.2681356
TAAGGTG1901.1859811E-825.00295
GTAAGGT1751.4808029E-724.4314064
ACGCAGA48150.023.7749757
GCGATTA601.7115936E-423.74786474-75
GAGTACT29250.023.70971112-13
CGCAGAG48450.023.6277568
GGTAAGG1652.4226338E-623.0329743
GCAGAGT51300.022.1299179
GTACTTT31000.022.0625314-15
GTACATG26450.021.9208371
CTAGACT1754.0372124E-621.7168054
AGTACTT30150.021.1901612-13
TACATGG26250.020.992912
Produced by FastQC (version 0.11.5)