FastQCFastQC Report
Thu 26 May 2016
SRR937296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687444
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34460.5012771949424244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27230.3961049918247886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.25049313107685867No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9540.13877494021331194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.044.6964451
TGTACCG652.5835252E-643.856795
GTATCAA35550.039.152871
ATCAACG43450.031.5922343
TCAACGC43900.031.277524
CAACGCA44200.031.0652285
TATCAAC44450.030.8860022
AACGCAG45400.030.3443436
GTCCTAC1303.0312913E-729.2335971
CGACACT700.00837933127.149444
CGCAGAG50950.026.6659748
ACGCAGA51700.026.2791397
TACATGG36950.025.3272132
GCAGAGT53150.025.2046959
GTACATG37650.025.108671
TATCACG1000.001652037423.7522962
ACATGGG37850.023.5919723
GAGTACT28700.023.420412-13
GTATTAG2554.8930815E-1022.3551031
CATGGGA26300.021.8589134
Produced by FastQC (version 0.11.5)