##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937296_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 687444 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.924898609923137 33.0 31.0 34.0 30.0 34.0 2 32.03845549601131 33.0 31.0 34.0 30.0 34.0 3 32.07564688905569 34.0 31.0 34.0 30.0 34.0 4 35.67560848592758 37.0 35.0 37.0 35.0 37.0 5 35.69073553627641 37.0 35.0 37.0 35.0 37.0 6 35.725803119963224 37.0 35.0 37.0 35.0 37.0 7 35.60694398380086 37.0 35.0 37.0 33.0 37.0 8 35.62740528683064 37.0 35.0 37.0 33.0 37.0 9 37.27769534682098 39.0 37.0 39.0 34.0 39.0 10-11 37.17948589266908 39.0 37.0 39.0 34.0 39.0 12-13 37.039891976655554 39.0 37.0 39.0 33.5 39.0 14-15 38.254387266453705 40.0 38.0 41.0 33.5 41.0 16-17 38.20181862086221 40.0 38.0 41.0 33.0 41.0 18-19 38.18659919935297 40.0 38.0 41.0 33.5 41.0 20-21 38.082539814152135 40.0 38.0 41.0 33.5 41.0 22-23 37.94170143313492 40.0 38.0 41.0 33.0 41.0 24-25 37.87922288942808 40.0 38.0 41.0 33.0 41.0 26-27 37.88979756896562 40.0 38.0 41.0 33.0 41.0 28-29 37.81347280651224 40.0 38.0 41.0 33.0 41.0 30-31 37.60133625429853 40.0 37.0 41.0 33.0 41.0 32-33 37.379910218141404 40.0 37.0 41.0 32.5 41.0 34-35 37.440581341898394 40.0 37.0 41.0 33.0 41.0 36-37 37.34158200522515 40.0 37.0 41.0 32.5 41.0 38-39 37.229693618680216 40.0 37.0 41.0 32.0 41.0 40-41 37.030035901106125 40.0 36.5 41.0 31.5 41.0 42-43 36.7674901519251 40.0 36.0 41.0 31.0 41.0 44-45 36.533652923001725 39.0 36.0 41.0 30.0 41.0 46-47 36.17837452941622 39.0 36.0 41.0 29.5 41.0 48-49 35.83270200918184 39.0 35.0 41.0 28.5 41.0 50-51 34.873647162532514 38.0 34.0 40.0 26.5 40.5 52-53 34.99948941295582 38.5 35.0 39.5 27.0 40.5 54-55 35.38271190089665 39.0 35.0 40.0 27.0 41.0 56-57 35.189561622473974 39.0 35.0 40.0 26.5 41.0 58-59 34.94232621129866 38.5 35.0 40.0 26.0 41.0 60-61 34.644088100267076 38.0 34.0 40.0 25.5 41.0 62-63 34.33681652614613 38.0 34.0 40.0 24.5 41.0 64-65 33.9244047515143 37.5 34.0 40.0 23.0 41.0 66-67 33.58329114807897 37.0 33.5 40.0 22.0 41.0 68-69 33.12484362362606 36.0 33.0 39.0 21.5 41.0 70-71 32.65675022256358 36.0 32.0 39.0 19.5 40.0 72-73 32.19606615229749 35.0 32.0 38.5 18.0 40.0 74-75 31.71466257615166 35.0 31.0 37.5 14.5 39.5 76-77 31.2194069626035 35.0 31.0 37.0 9.5 39.0 78-79 30.709049755325523 35.0 30.5 36.5 7.0 39.0 80-81 30.220543055143402 34.0 30.0 36.0 4.0 38.5 82-83 29.749450864361314 34.0 29.5 35.5 2.0 37.0 84-85 29.224613204857413 34.0 29.0 35.0 2.0 37.0 86-87 28.74997308871704 34.0 29.0 35.0 2.0 36.0 88-89 28.29586555413968 34.0 28.5 35.0 2.0 36.0 90-91 27.78754560371463 33.5 27.0 35.0 2.0 35.5 92-93 27.293037687433447 33.0 26.0 35.0 2.0 35.0 94-95 26.77283822391351 33.0 25.5 35.0 2.0 35.0 96-97 26.067145832969665 32.5 24.5 35.0 2.0 35.0 98-99 25.16982765141597 32.0 20.0 34.0 2.0 35.0 100-101 22.654758060292913 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2173524548178527 1101 2 -0.12889450293396365 1101 3 -0.0022984360527615877 1101 4 -0.009474209649752652 1101 5 -0.08068904241710584 1101 6 -0.20157803219203174 1101 7 -0.23168421972916065 1101 8 -0.3805158313538328 1101 9 -0.35571525655542047 1101 10-11 -0.33139580041328287 1101 12-13 -0.2593462701656577 1101 14-15 -0.21360669599916804 1101 16-17 -0.42659466442561467 1101 18-19 -0.20383358383612915 1101 20-21 -0.3668012167288168 1101 22-23 -0.3462126651574522 1101 24-25 -0.41392219506433037 1101 26-27 -0.4620392832750255 1101 28-29 -0.4185224462717869 1101 30-31 -0.3753504937365477 1101 32-33 -0.5190005168915519 1101 34-35 -0.5974023986028953 1101 36-37 -0.41401200487165113 1101 38-39 -0.3816908375045216 1101 40-41 -0.5427069163899318 1101 42-43 -0.3672908793178493 1101 44-45 -0.030439063202067018 1101 46-47 -0.08038778663836155 1101 48-49 -0.2194182440241832 1101 50-51 -0.11554739992688923 1101 52-53 -0.15877682917614777 1101 54-55 -0.1484477851306778 1101 56-57 -0.10578234724377467 1101 58-59 -0.08575927777790326 1101 60-61 0.17109304116268476 1101 62-63 0.10230677603212968 1101 64-65 -0.25535492003053406 1101 66-67 -0.37702377406012744 1101 68-69 -0.1626311980280164 1101 70-71 -0.4537819728270023 1101 72-73 -0.17615490885778584 1101 74-75 -0.1433155161484514 1101 76-77 -0.0678413306121044 1101 78-79 -0.10263062077590135 1101 80-81 0.1745292769787561 1101 82-83 0.12019213094711745 1101 84-85 -0.028021905181095264 1101 86-87 -0.09595685456035952 1101 88-89 -0.1707345326233458 1101 90-91 -0.25913255755774145 1101 92-93 -0.1226509321732081 1101 94-95 -0.2962272405137938 1101 96-97 0.054108412668369965 1101 98-99 0.16030653802622652 1101 100-101 0.23689882141715302 1102 1 -0.23573672859005868 1102 2 -0.12967820199352786 1102 3 -0.1756374294348575 1102 4 -0.20569286168736767 1102 5 -0.1517770779447929 1102 6 -0.3233343888621789 1102 7 -0.28192803721435666 1102 8 -0.17853916817027482 1102 9 -0.27351393297478666 1102 10-11 -0.28985430790196887 1102 12-13 -0.31314938754636046 1102 14-15 -0.1823077148079406 1102 16-17 -0.20851951639356514 1102 18-19 -0.4421325214885172 1102 20-21 -0.3827309885853154 1102 22-23 -0.3727278166726009 1102 24-25 -0.44997779415871975 1102 26-27 -0.7330794260824121 1102 28-29 -0.511031589427482 1102 30-31 -0.31978405347183525 1102 32-33 -0.46414158272227013 1102 34-35 -0.21832063266767676 1102 36-37 -0.2613648436038005 1102 38-39 -0.1098506685738343 1102 40-41 -0.3603336144045599 1102 42-43 -0.20999050488385507 1102 44-45 -0.3008914317147813 1102 46-47 -0.13489296775992443 1102 48-49 -0.15501341992108308 1102 50-51 -0.20917222577154604 1102 52-53 -0.02364316494765717 1102 54-55 -0.11049280428776598 1102 56-57 -0.18949120304314704 1102 58-59 -0.16832523911542552 1102 60-61 -0.10336490032111811 1102 62-63 0.18319649215891332 1102 64-65 -0.117636137376401 1102 66-67 -0.5126907890374852 1102 68-69 -0.5843538120997707 1102 70-71 -0.5740307972784144 1102 72-73 -0.3558505723896559 1102 74-75 -0.6215080013452479 1102 76-77 -0.3762541223223117 1102 78-79 -0.21896094992950665 1102 80-81 -0.1573509476816426 1102 82-83 -0.47800971509955303 1102 84-85 -0.40818125732320354 1102 86-87 -0.3939257678309964 1102 88-89 -0.2942324427591849 1102 90-91 -0.5895433464814595 1102 92-93 -0.6369153435281376 1102 94-95 -0.7749694035232011 1102 96-97 -0.5483656817238263 1102 98-99 -0.3923700119563591 1102 100-101 -0.19666635622130357 1103 1 -0.18458730330270257 1103 2 -0.08928411332357555 1103 3 -0.047996488000812576 1103 4 -0.08488005380559827 1103 5 -0.16864899912706477 1103 6 -0.20401309712709548 1103 7 -0.1866625747075119 1103 8 -0.266771242609245 1103 9 -0.3096113604515267 1103 10-11 -0.11455326794575171 1103 12-13 -0.17549886756825828 1103 14-15 -0.3663799643974315 1103 16-17 -0.34437063845158633 1103 18-19 -0.11765934141189405 1103 20-21 -0.13924439854700665 1103 22-23 -0.10665638810117883 1103 24-25 -0.16041840718553857 1103 26-27 -0.3226182876040298 1103 28-29 -0.2607031822025192 1103 30-31 -0.2715355586716086 1103 32-33 -0.22387064676168222 1103 34-35 -0.2043017492522452 1103 36-37 -0.3002674161270633 1103 38-39 -0.5341664868551703 1103 40-41 -0.5377556176886316 1103 42-43 -0.4911139312659074 1103 44-45 -0.44870205021505427 1103 46-47 -0.09260369572927374 1103 48-49 -0.11728079813673986 1103 50-51 -0.1987589800134728 1103 52-53 -0.33981037895969735 1103 54-55 -0.22969778513067496 1103 56-57 -0.35823094031736247 1103 58-59 -0.39240159379522055 1103 60-61 -0.34094700212735773 1103 62-63 -0.17736043176007144 1103 64-65 0.14703956048894895 1103 66-67 0.006958909922559542 1103 68-69 0.10640289288107851 1103 70-71 0.025722897302863146 1103 72-73 -0.0670369434898177 1103 74-75 -0.06378358974152576 1103 76-77 0.025381071985297154 1103 78-79 -0.13546341298369313 1103 80-81 -0.08701022518574675 1103 82-83 -0.17791392965894204 1103 84-85 -0.07558684024602869 1103 86-87 0.006505266651760877 1103 88-89 0.32775031586150405 1103 90-91 0.43005845975827484 1103 92-93 0.47430523665796187 1103 94-95 0.2174361793996269 1103 96-97 0.23006891050386713 1103 98-99 0.3842378150824999 1103 100-101 0.9582597521530829 1104 1 0.1829894885061698 1104 2 0.15544203652202526 1104 3 0.1811518949018378 1104 4 0.0794666063601781 1104 5 0.19100076521553433 1104 6 0.24844911642726686 1104 7 0.28799206530548815 1104 8 0.2804496252600899 1104 9 0.2840096374356875 1104 10-11 0.3447389472338358 1104 12-13 0.16996066436738744 1104 14-15 0.2611919517410328 1104 16-17 0.21050909501176562 1104 18-19 0.23707615346865651 1104 20-21 0.28117653433126577 1104 22-23 0.02317662018746347 1104 24-25 0.216515608741652 1104 26-27 0.2653530272017548 1104 28-29 0.3321336453612673 1104 30-31 0.18229184259606512 1104 32-33 0.20827221038117472 1104 34-35 0.31938385126782975 1104 36-37 0.35360874184465274 1104 38-39 0.3309846589273775 1104 40-41 0.6228123979135134 1104 42-43 0.6597494687015981 1104 44-45 0.6879101714654254 1104 46-47 0.704651708138762 1104 48-49 0.9249445390971331 1104 50-51 0.7338795702871934 1104 52-53 0.7681836889746378 1104 54-55 0.7505496551423647 1104 56-57 0.8839415771537844 1104 58-59 0.5917910764469312 1104 60-61 0.8016358842695226 1104 62-63 1.0125692773249213 1104 64-65 1.1796354486735723 1104 66-67 1.060961022713066 1104 68-69 0.9659449861688998 1104 70-71 1.1500728079155706 1104 72-73 1.1105248150019733 1104 74-75 1.2114215947213332 1104 76-77 1.0958406039209407 1104 78-79 1.1078406663272098 1104 80-81 1.0609074572455874 1104 82-83 0.9148903934354848 1104 84-85 1.411882686813943 1104 86-87 1.3311781904287798 1104 88-89 1.3470478130173653 1104 90-91 1.3269075577592524 1104 92-93 1.5020274859672433 1104 94-95 1.461280645513714 1104 96-97 1.6152590616496525 1104 98-99 1.7541163296282534 1104 100-101 1.5247598334142545 1105 1 -0.022405328313482187 1105 2 -0.08529224490738585 1105 3 -0.019908642254492293 1105 4 -0.037739968793587764 1105 5 -0.0954693650483307 1105 6 -0.14542873194506 1105 7 -0.1051896491854265 1105 8 -0.1397429668746284 1105 9 -0.2099039742628932 1105 10-11 -0.25916455359843127 1105 12-13 -0.2553293055649277 1105 14-15 -0.3012687711491111 1105 16-17 -0.384740563912068 1105 18-19 -0.09855088949295521 1105 20-21 -0.2144707892326494 1105 22-23 -0.11313316380498861 1105 24-25 -0.05204195181684668 1105 26-27 -0.1889530686056915 1105 28-29 -0.2715314332959764 1105 30-31 -0.09384813182506235 1105 32-33 -0.12074891941615107 1105 34-35 -0.11422366756679025 1105 36-37 -0.030181788085414496 1105 38-39 -0.2795755301620204 1105 40-41 -0.2127094155078879 1105 42-43 -0.1958042226476593 1105 44-45 -0.09201659466155121 1105 46-47 -0.18842624855376755 1105 48-49 -0.06708597894127166 1105 50-51 -0.17334624053216174 1105 52-53 -0.1358131202890931 1105 54-55 -0.21217406801122252 1105 56-57 -0.2879674338798637 1105 58-59 -0.10519420760659415 1105 60-61 0.17016077215958347 1105 62-63 0.18478073130281558 1105 64-65 -0.06909039084475666 1105 66-67 0.025672333196133934 1105 68-69 -0.17365855601931202 1105 70-71 -0.20623515111702062 1105 72-73 -0.08311299227932523 1105 74-75 -0.10913334024975185 1105 76-77 -0.1873622594999489 1105 78-79 -0.12187689169988758 1105 80-81 -0.03393084983922989 1105 82-83 0.14208391423929356 1105 84-85 0.04400904467973987 1105 86-87 0.2130113037367103 1105 88-89 0.1142800700659059 1105 90-91 0.30533182266100667 1105 92-93 0.2713282761454252 1105 94-95 0.0969023902047752 1105 96-97 0.1548871819262807 1105 98-99 0.30272869785216727 1105 100-101 0.5004069214994757 1106 1 -0.06451775536429949 1106 2 0.06149297843712276 1106 3 -0.019918282053751568 1106 4 0.11631751911328791 1106 5 0.09776041113480716 1106 6 -0.11076208584329805 1106 7 -0.4061750294658708 1106 8 -0.1987597459092214 1106 9 -0.08721053723103012 1106 10-11 -0.27070435729356035 1106 12-13 0.02814441537830703 1106 14-15 0.008799227994877867 1106 16-17 0.0388331795883019 1106 18-19 -0.13905154210736725 1106 20-21 -0.10131593356936008 1106 22-23 0.011276157495302641 1106 24-25 -0.029382639674359723 1106 26-27 -0.19879723161084684 1106 28-29 0.20584106589910078 1106 30-31 -0.09711216785478172 1106 32-33 0.05948929930318059 1106 34-35 0.49234238530172547 1106 36-37 0.22986529269417133 1106 38-39 0.28968739150690226 1106 40-41 0.2899588809275073 1106 42-43 0.4843375980790725 1106 44-45 0.5450546029678307 1106 46-47 0.5106066867843921 1106 48-49 0.8924018006851995 1106 50-51 0.646367341752196 1106 52-53 0.6745720282505729 1106 54-55 0.559528494054625 1106 56-57 0.5817564629897092 1106 58-59 0.6613144306175158 1106 60-61 0.6531937614806225 1106 62-63 0.6985328689283037 1106 64-65 0.7697553010325535 1106 66-67 0.6738331558951671 1106 68-69 0.37888798975141924 1106 70-71 0.4057793162937102 1106 72-73 0.5906887324735592 1106 74-75 0.7686706363602447 1106 76-77 0.8472185923883906 1106 78-79 0.6344145232876848 1106 80-81 0.2799766813503375 1106 82-83 0.4741104121195008 1106 84-85 0.3678735245258089 1106 86-87 0.25487213202682213 1106 88-89 0.6208389894829658 1106 90-91 0.5106373753781774 1106 92-93 1.0047164920692175 1106 94-95 1.0220011499482027 1106 96-97 1.2285448880782077 1106 98-99 0.9745995708131119 1106 100-101 0.9898561612085075 1107 1 -0.06580505356069821 1107 2 -0.06690463521654166 1107 3 -0.043581037610131546 1107 4 -0.16213569290334107 1107 5 0.05546643652088079 1107 6 -0.06491535276619231 1107 7 -3.069798379868871E-4 1107 8 -0.05542670479411527 1107 9 -0.09288572282805063 1107 10-11 0.17381839650951036 1107 12-13 -0.06905813337984057 1107 14-15 0.14426429330564616 1107 16-17 0.12060429889177016 1107 18-19 -0.03439050756256279 1107 20-21 0.06694755191739432 1107 22-23 0.11153430923038599 1107 24-25 -0.11467537312981335 1107 26-27 0.0468587137228198 1107 28-29 -0.020252791519936864 1107 30-31 -0.04554410946035148 1107 32-33 -0.1758867163474065 1107 34-35 -0.1560764064835567 1107 36-37 -0.3487267998805663 1107 38-39 -0.06829224242055432 1107 40-41 0.10513469633171013 1107 42-43 0.3536652962145652 1107 44-45 0.029747750757969982 1107 46-47 0.2535392351272847 1107 48-49 0.49018455642613645 1107 50-51 0.5018196725001687 1107 52-53 0.2722878813970695 1107 54-55 0.009221203517419951 1107 56-57 0.19752794218300096 1107 58-59 0.5164680066765399 1107 60-61 0.5689287902948763 1107 62-63 0.45628434193887557 1107 64-65 0.5338798229097037 1107 66-67 0.2813986858329258 1107 68-69 0.4365499517046061 1107 70-71 0.7060081693064078 1107 72-73 0.770706311706995 1107 74-75 0.7396164544866899 1107 76-77 0.67054729636979 1107 78-79 0.8270605439675087 1107 80-81 0.8974380277996588 1107 82-83 0.606477195212058 1107 84-85 0.6474929179730502 1107 86-87 0.9701404575702348 1107 88-89 0.6833617709698636 1107 90-91 0.6967453976913447 1107 92-93 0.5192816482752391 1107 94-95 1.013009671954542 1107 96-97 0.880087338927872 1107 98-99 0.9422117941478128 1107 100-101 1.4632612264270044 1108 1 -0.025757281227647866 1108 2 0.043591951009439356 1108 3 -0.02224751291409177 1108 4 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0.7876548913425623 2316 42-43 0.25217424607399863 2316 44-45 0.5980549284384793 2316 46-47 0.6256434307075054 2316 48-49 -0.378672423752505 2316 50-51 -0.7187507698328446 2316 52-53 -0.49730216877907196 2316 54-55 -0.7694966357053872 2316 56-57 -0.564491203043147 2316 58-59 -0.15539420463266396 2316 60-61 -0.1795143256084728 2316 62-63 -0.2511904810211618 2316 64-65 -0.45959016036491107 2316 66-67 -0.8081888733286675 2316 68-69 -0.3793729691878909 2316 70-71 -0.5553526363588759 2316 72-73 -0.6159080436540236 2316 74-75 -0.14976470632609207 2316 76-77 -0.2115031644679064 2316 78-79 0.19483215351877092 2316 80-81 -0.19614405112991662 2316 82-83 -0.7471668032221572 2316 84-85 -0.02599734927722963 2316 86-87 -0.01892576783099642 2316 88-89 -0.03704853471320746 2316 90-91 -0.2878192085504274 2316 92-93 -1.1948655350990194 2316 94-95 -0.2835900931783719 2316 96-97 -0.2461626165897286 2316 98-99 0.6066721336375096 2316 100-101 0.5490232989511092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2638.0 3 553.0 4 395.0 5 405.0 6 514.0 7 606.0 8 677.0 9 886.0 10 1141.0 11 1495.0 12 2109.0 13 2777.0 14 3206.0 15 4222.0 16 5362.0 17 7519.0 18 6634.0 19 4202.0 20 4088.0 21 4183.0 22 4573.0 23 5044.0 24 5429.0 25 6297.0 26 7283.0 27 8475.0 28 10207.0 29 12503.0 30 15210.0 31 18912.0 32 24710.0 33 33080.0 34 45684.0 35 65053.0 36 97354.0 37 142198.0 38 117204.0 39 14614.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.311111888764465 19.4662187383352 15.162199048152294 22.060470324748042 2 14.623600738789511 20.296302991164556 41.56361563703102 23.51648063301491 3 18.464397638656074 25.57787333284746 30.66948473143357 25.288244297062896 4 11.955830903790087 16.89271137026239 38.467346938775506 32.68411078717201 5 12.88227748462144 37.94699862977755 34.88936182618583 14.281362059415178 6 30.235148659209404 37.068281758482726 18.40277372773611 14.293795854571753 7 27.458273613117985 33.513065057732376 21.407127349501412 17.621533979648227 8 25.92156308178567 34.99191604937552 20.392840637560557 18.693680231278258 9 26.28349507769415 16.574022645668325 20.97239047814811 36.17009179848941 10-11 23.945672460048993 27.391884404525836 28.04415023912283 20.618292896302346 12-13 25.115845034483762 24.85871134246096 28.607380837816955 21.418062785238323 14-15 22.18118442517199 25.76780041781288 27.39298465055186 24.658030506463273 16-17 20.99042281203502 29.301514838918507 27.849517787091465 21.85854456195501 18-19 20.954102626716185 27.564374045551126 30.761117165318748 20.72040616241394 20-21 21.85788446315618 26.540279869322397 30.835482380245 20.766353287276427 22-23 21.438275888207958 26.661617987486665 30.716863851845805 21.183242272459577 24-25 21.1180391313696 26.736295092362134 30.930180861414897 21.215484914853374 26-27 21.02510162890525 27.023266222174602 30.729504928555446 21.222127220364705 28-29 20.861224766499227 27.064075396712582 30.96206036139862 21.112639475389567 30-31 21.09950765920161 26.877113078012034 30.869563949631658 21.1538153131547 32-33 20.803051133605184 27.021150035937712 30.663761519385226 21.51203731107188 34-35 20.977164835421185 27.041853290409872 30.671247696363167 21.309734177805773 36-37 21.132156336235738 26.831568712344183 30.582244749505456 21.454030201914627 38-39 21.006673644497962 26.986641048890498 30.699639349137442 21.307045957474102 40-41 21.224847684860908 27.045658462774174 30.55960279622825 21.169891056136667 42-43 20.98504832162618 27.120098137472638 30.360223710287453 21.53462983061373 44-45 21.081660726613453 27.24935320365574 30.176539499227633 21.49244657050317 46-47 21.187814068977985 27.375843197520183 29.671877426254618 21.764465307247217 48-49 21.199716550725608 27.433559285952786 29.546743614007408 21.819980549314195 50-51 21.253974120918258 27.660173202778772 29.396356457413393 21.689496218889587 52-53 21.371040657994516 27.73486262614478 29.123986466779446 21.770110249081256 54-55 21.236040000379095 27.696260152644154 29.208631415184367 21.859068431792384 56-57 21.338908779213508 27.905825791855204 28.970427881699866 21.784837547231422 58-59 21.42863910155268 27.577697692299285 29.185909652405538 21.807753553742497 60-61 21.417218166593425 27.458100945438858 29.445031107729598 21.67964978023812 62-63 21.24606623161387 27.540314293335587 29.49717730709412 21.716442167956423 64-65 21.163199834419952 27.567183084015838 29.534174934737266 21.73544214682694 66-67 21.045021724523423 27.783559323763175 29.248889724540916 21.92252922717249 68-69 21.015949829278156 27.68572605065902 29.30789328882783 21.990430831234992 70-71 20.99441594843607 27.618243858782236 29.236248671765342 22.151091521016355 72-73 20.954128065921363 27.645822810826647 29.118937305174192 22.281111818077797 74-75 20.996976870288204 27.815927258309962 29.021535061891075 22.165560809510758 76-77 21.12553273218814 27.54033166245972 29.115039538987574 22.219096066364564 78-79 21.047713729285956 27.586412986437477 29.06066693099789 22.305206353278678 80-81 20.92614430627366 27.78862931050819 29.03785127990589 22.24737510331226 82-83 20.96672747401233 27.655705553486538 28.956710660283232 22.420856312217904 84-85 21.044012267577795 27.478222717175193 29.04468570162499 22.433079313622027 86-87 21.05338728815783 27.49626096564753 28.99916327262444 22.451188473570202 88-89 20.974638808737538 27.30783050558395 28.942092055942283 22.77543862973623 90-91 20.851418970877113 27.194311725324265 29.27471582315618 22.679553480642443 92-93 20.896379571087586 27.18867408851557 29.242570559189552 22.672375781207297 94-95 20.72426630284267 27.078577821312273 29.1409759695414 23.056179906303658 96-97 20.715572685555017 27.102927713864222 29.0250234695246 23.15647613105616 98-99 20.694918219190065 27.021851949036414 29.089915157876323 23.1933146738972 100-101 20.78560465716247 26.739038862644897 29.502188510719545 22.97316796947308 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 919.0 1 554.0 2 169.0 3 154.0 4 134.0 5 131.5 6 206.0 7 423.0 8 729.0 9 894.0 10 877.5 11 872.5 12 946.5 13 1001.0 14 1074.5 15 1177.5 16 1227.5 17 1266.5 18 1346.5 19 1446.0 20 1614.5 21 1853.0 22 2119.5 23 2476.5 24 2962.0 25 3618.0 26 4520.0 27 5637.5 28 6788.5 29 8189.0 30 9778.0 31 11074.5 32 12217.0 33 13705.5 34 15807.5 35 17447.5 36 19069.0 37 20946.0 38 21940.0 39 22470.0 40 23056.5 41 23846.0 42 24421.5 43 25156.0 44 26108.0 45 26527.0 46 26331.5 47 26197.5 48 27225.0 49 27187.5 50 25563.0 51 23859.0 52 21986.0 53 20541.0 54 19249.0 55 17237.0 56 14881.5 57 12970.0 58 11681.5 59 10459.0 60 8887.0 61 7022.5 62 5093.5 63 3619.5 64 2567.0 65 1847.0 66 1316.0 67 984.0 68 722.0 69 457.5 70 302.0 71 217.0 72 157.5 73 125.0 74 90.5 75 64.0 76 52.5 77 35.0 78 19.5 79 15.0 80 11.5 81 7.0 82 4.0 83 3.5 84 2.5 85 1.0 86 2.0 87 3.0 88 3.5 89 2.5 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23565555885279385 2 0.21194453657316087 3 0.20278015372888555 4 0.2100534734465644 5 0.20714414555949284 6 0.22983690307865076 7 0.23376449572619734 8 0.22067252023437547 9 0.21601759561506098 10-11 0.2333280965431366 12-13 0.23478276048667238 14-15 0.21689039398118246 16-17 0.2320916321911312 18-19 0.21987245506543077 20-21 0.2154357300376467 22-23 0.2117990701788073 24-25 0.2067804795736089 26-27 0.21710859357271284 28-29 0.22190898458638086 30-31 0.223581848121447 32-33 0.22329091533273984 34-35 0.22867317192382217 36-37 0.21252640215057517 38-39 0.21267186854492878 40-41 0.21070807222115548 42-43 0.21347193371387344 44-45 0.22743670757181678 46-47 0.2309279010363026 48-49 0.23376449572619734 50-51 0.2328189641628991 52-53 0.2507840638655658 54-55 0.2328189641628991 56-57 0.21238093575622158 58-59 0.1995071598559301 60-61 0.19790702951804073 62-63 0.2032165529119463 64-65 0.19943442665875327 66-67 0.1968160315603889 68-69 0.20168915577123375 70-71 0.2011800233909962 72-73 0.20576221481313386 74-75 0.203652952095007 76-77 0.21463566486870203 78-79 0.20888974229173576 80-81 0.2061258807990178 82-83 0.20328928610912306 84-85 0.20481668324983565 86-87 0.20918067508044294 88-89 0.20590768120748743 90-91 0.21405379929128773 92-93 0.20459848365830527 94-95 0.20336201930629985 96-97 0.21063533902397868 98-99 0.2129628013336359 100-101 0.19797976271521753 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 687444.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.85187291523111 #Duplication Level Percentage of deduplicated Percentage of total 1 83.68009868111058 56.77851421244714 2 9.884185714568325 13.413210259508656 3 2.692534204735458 5.480804660388696 4 1.2135456562362583 3.2936538257509342 5 0.6427462966765266 2.1805770019415562 6 0.40535279970955357 1.6502367991035456 7 0.28472136146516036 1.352321434406995 8 0.20396828977320647 1.107170438114291 9 0.1346993409104003 0.8225642305096086 >10 0.8074820259978456 9.673012195771536 >50 0.039072776459381516 1.7593759076738147 >100 0.010519440102485924 1.106770753135141 >500 4.293649019426072E-4 0.23233737176794186 >1k 6.440473529139108E-4 1.1494509094801435 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3446 0.5012771949424244 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2723 0.3961049918247886 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1722 0.25049313107685867 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 954 0.13877494021331194 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.4546639435357643E-4 0.0 0.0 0.0 0.0 6 1.4546639435357643E-4 0.0 0.0 0.0 0.0 7 1.4546639435357643E-4 0.0 0.0 0.0 0.0 8 1.4546639435357643E-4 0.0 0.0 0.0 0.0 9 1.4546639435357643E-4 0.0 0.0 0.0 0.0 10-11 1.4546639435357643E-4 0.0 0.0 0.0 0.0 12-13 1.4546639435357643E-4 0.0 0.0 0.0 1.4546639435357643E-4 14-15 1.4546639435357643E-4 0.0 0.0 0.0 1.4546639435357643E-4 16-17 1.4546639435357643E-4 0.0 0.0 0.0 1.4546639435357643E-4 18-19 1.4546639435357643E-4 0.0 0.0 0.0 1.4546639435357643E-4 20-21 1.4546639435357643E-4 0.0 0.0 0.0 1.4546639435357643E-4 22-23 1.4546639435357643E-4 0.0 0.0 2.1819959153036465E-4 1.4546639435357643E-4 24-25 1.4546639435357643E-4 0.0 0.0 8.727983661214586E-4 1.4546639435357643E-4 26-27 1.4546639435357643E-4 0.0 0.0 0.0016001303378893408 1.4546639435357643E-4 28-29 2.1819959153036465E-4 0.0 0.0 0.00327299387295547 1.4546639435357643E-4 30-31 2.9093278870715285E-4 0.0 0.0 0.007491519309209186 1.4546639435357643E-4 32-33 2.9093278870715285E-4 0.0 0.0 0.014037507055120127 1.4546639435357643E-4 34-35 5.091323802375175E-4 0.0 0.0 0.01920156405467209 1.4546639435357643E-4 36-37 7.273319717678822E-4 0.0 0.0 0.027056749349765218 1.4546639435357643E-4 38-39 7.273319717678822E-4 0.0 0.0 0.037384863348869146 1.4546639435357643E-4 40-41 7.273319717678822E-4 0.0 0.0 0.05862295692449131 1.4546639435357643E-4 42-43 7.273319717678822E-4 0.0 0.0 0.07891551893681521 1.4546639435357643E-4 44-45 7.273319717678822E-4 0.0 0.0 0.10284474080797854 1.4546639435357643E-4 46-47 0.001018264760475035 0.0 0.0 0.14917578740959264 1.4546639435357643E-4 48-49 0.001018264760475035 0.0 0.0 0.18263305811091524 1.4546639435357643E-4 50-51 0.001018264760475035 0.0 0.0 0.22561837764239706 1.4546639435357643E-4 52-53 0.001018264760475035 0.0 0.0 0.27551335090567375 1.4546639435357643E-4 54-55 0.001018264760475035 0.0 0.0 0.3442462222377386 1.4546639435357643E-4 56-57 0.001018264760475035 0.0 0.0 0.49873153304123685 1.4546639435357643E-4 58-59 0.001018264760475035 0.0 0.0 0.6428159966484542 1.4546639435357643E-4 60-61 0.001018264760475035 0.0 0.0 0.7713355560598391 1.4546639435357643E-4 62-63 0.001018264760475035 0.0 0.0 0.9302575918911213 1.4546639435357643E-4 64-65 0.001018264760475035 0.0 0.0 1.090707024863116 1.4546639435357643E-4 66-67 0.001018264760475035 0.0 0.0 1.3168345348857509 1.4546639435357643E-4 68-69 0.001018264760475035 0.0 0.0 1.5882748267495241 1.4546639435357643E-4 70-71 0.001090997957651823 0.0 0.0 1.772435282001152 1.4546639435357643E-4 72-73 0.0011637311548286114 0.0 0.0 1.9951443317564777 1.4546639435357643E-4 74-75 0.0011637311548286114 0.0 0.0 2.3361902933184377 1.4546639435357643E-4 76-77 0.0011637311548286114 0.0 0.0 2.7496785192684783 1.4546639435357643E-4 78-79 0.0011637311548286114 0.0 0.0 3.147674574219864 1.4546639435357643E-4 80-81 0.0011637311548286114 0.0 0.0 3.4653731794880747 1.4546639435357643E-4 82-83 0.0011637311548286114 0.0 0.0 3.9150098044349795 1.4546639435357643E-4 84-85 0.0011637311548286114 0.0 0.0 4.490983992877965 1.4546639435357643E-4 86-87 0.0011637311548286114 0.0 0.0 5.015244878128255 1.4546639435357643E-4 88-89 0.0011637311548286114 0.0 0.0 5.639368443102274 1.4546639435357643E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2030 0.0 44.696445 1 TGTACCG 65 2.5835252E-6 43.85679 5 GTATCAA 3555 0.0 39.15287 1 ATCAACG 4345 0.0 31.592234 3 TCAACGC 4390 0.0 31.27752 4 CAACGCA 4420 0.0 31.065228 5 TATCAAC 4445 0.0 30.886002 2 AACGCAG 4540 0.0 30.344343 6 GTCCTAC 130 3.0312913E-7 29.233597 1 CGACACT 70 0.008379331 27.14944 4 CGCAGAG 5095 0.0 26.665974 8 ACGCAGA 5170 0.0 26.279139 7 TACATGG 3695 0.0 25.327213 2 GCAGAGT 5315 0.0 25.204695 9 GTACATG 3765 0.0 25.10867 1 TATCACG 100 0.0016520374 23.752296 2 ACATGGG 3785 0.0 23.591972 3 GAGTACT 2870 0.0 23.4204 12-13 GTATTAG 255 4.8930815E-10 22.355103 1 CATGGGA 2630 0.0 21.858913 4 >>END_MODULE