FastQCFastQC Report
Thu 26 May 2016
SRR937296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687444
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.6457253245355258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37970.5523358993605297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.3582837292928588No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.13921133939637265No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25900.047.5187721
GTATCAA43400.035.8033181
TATCAAC54600.028.4466272
ATCAACG55050.028.127813
TCAACGC55500.027.8997484
CAACGCA56800.027.344825
AACGCAG57800.026.8717256
ATAGACG908.9460344E-426.3877963
TAAGGTG2551.8189894E-1124.2146855
ACGCAGA66050.023.4468027
GAGTACT40550.023.25457812-13
CGCAGAG66250.023.1609028
TACCTGG5200.022.8355942
GTACCTG5950.022.3617761
GCAGAGT69250.021.9517449
GTACTTT42800.021.917914-15
AGTACTT41750.021.84658812-13
GTACTAC1353.783692E-421.1194551
CTTAGAC1908.246274E-619.9991743
GTCTAGG2401.4052421E-719.799491
Produced by FastQC (version 0.11.5)