##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937296_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 687444 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41582441624336 33.0 31.0 34.0 31.0 34.0 2 32.61614909723556 34.0 31.0 34.0 31.0 34.0 3 32.66695614479143 34.0 31.0 34.0 31.0 34.0 4 36.263733482290924 37.0 35.0 37.0 35.0 37.0 5 36.20800239728618 37.0 35.0 37.0 35.0 37.0 6 36.26118491106185 37.0 37.0 37.0 35.0 37.0 7 36.21281297094745 37.0 36.0 37.0 35.0 37.0 8 36.18985400992663 37.0 36.0 37.0 35.0 37.0 9 37.93169770919522 39.0 38.0 39.0 35.0 39.0 10-11 37.85829027528061 39.0 38.0 39.0 35.0 39.0 12-13 37.77514386626402 39.0 37.0 39.0 35.0 39.0 14-15 39.01868152169486 40.0 38.0 41.0 36.0 41.0 16-17 38.98730805709265 40.0 38.0 41.0 36.0 41.0 18-19 39.003219171307045 40.0 38.0 41.0 36.0 41.0 20-21 38.921285224687395 40.0 38.0 41.0 35.0 41.0 22-23 38.782819400562076 40.0 38.0 41.0 35.0 41.0 24-25 38.72490341031415 40.0 38.0 41.0 35.0 41.0 26-27 38.6837953346018 40.0 38.0 41.0 35.0 41.0 28-29 38.55515794741099 40.0 38.0 41.0 34.0 41.0 30-31 38.62374317035278 40.0 38.0 41.0 35.0 41.0 32-33 38.550868143441505 40.0 38.0 41.0 35.0 41.0 34-35 38.42537501236464 40.0 38.0 41.0 34.5 41.0 36-37 38.27463546121575 40.0 38.0 41.0 34.0 41.0 38-39 38.14501326653516 40.0 38.0 41.0 34.0 41.0 40-41 37.96322464084348 40.0 38.0 41.0 34.0 41.0 42-43 37.73939404518768 40.0 37.0 41.0 33.0 41.0 44-45 37.426156894234296 40.0 37.0 41.0 33.0 41.0 46-47 37.00611177055876 40.0 37.0 41.0 31.5 41.0 48-49 36.6137983603028 40.0 37.0 41.0 31.0 41.0 50-51 36.359874404315114 40.0 36.0 41.0 30.0 41.0 52-53 36.12158590372452 39.0 36.0 41.0 30.0 41.0 54-55 35.905939974747035 39.0 35.5 41.0 29.0 41.0 56-57 35.6602225053968 39.0 35.0 40.0 28.5 41.0 58-59 35.39668103874643 39.0 35.0 40.0 28.0 41.0 60-61 35.08495819295827 38.0 35.0 40.0 27.5 41.0 62-63 34.7237658631103 38.0 34.0 40.0 26.5 41.0 64-65 34.34706026963651 37.5 34.0 40.0 26.0 41.0 66-67 33.952339390553995 37.0 34.0 39.5 26.0 41.0 68-69 33.52038929716457 36.0 33.0 39.0 25.0 40.5 70-71 33.12149571456002 36.0 33.0 39.0 24.0 40.0 72-73 32.65254478910282 35.0 32.5 38.0 22.5 40.0 74-75 32.16751255374983 35.0 32.0 37.5 21.5 39.5 76-77 30.9114102385067 34.0 30.5 36.0 20.0 38.5 78-79 31.48743097619588 35.0 31.5 36.0 20.0 39.0 80-81 31.280171912184848 35.0 32.0 36.0 19.5 38.0 82-83 30.946415271643943 34.5 31.0 36.0 18.0 37.0 84-85 30.58225397268723 34.0 31.0 35.0 16.5 37.0 86-87 30.181666433920434 34.0 31.0 35.0 9.0 36.0 88-89 29.75091862028034 34.0 30.0 35.0 6.0 36.0 90-91 29.286947009501866 34.0 30.0 35.0 2.0 35.5 92-93 28.95251904155102 34.0 29.0 35.0 2.0 35.0 94-95 28.55858077167013 34.0 29.0 35.0 2.0 35.0 96-97 28.118765310338006 34.0 29.0 35.0 2.0 35.0 98-99 27.43097255921937 33.5 28.0 35.0 2.0 35.0 100-101 25.017582523085515 31.0 21.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.17720325536978976 1101 2 0.005918180513837967 1101 3 -0.06250090570211597 1101 4 -0.0051186585316003175 1101 5 -0.06336364806158201 1101 6 -0.08794884272844428 1101 7 -0.07478726632963628 1101 8 -0.12293287764759953 1101 9 -0.136675358495971 1101 10-11 -0.15908963632427486 1101 12-13 -0.14519318971776585 1101 14-15 -0.3078323571824839 1101 16-17 -0.2716333654161218 1101 18-19 -0.1332453731833425 1101 20-21 -0.2325679335570996 1101 22-23 -0.20255916024183307 1101 24-25 -0.26657169853474727 1101 26-27 -0.2796653164346097 1101 28-29 -0.254510169886494 1101 30-31 -0.23504324474316718 1101 32-33 -0.1383743633435941 1101 34-35 -0.12338624689958522 1101 36-37 -0.15918754800980395 1101 38-39 -0.20149634766048763 1101 40-41 -0.04834957397110884 1101 42-43 0.19113215651529458 1101 44-45 0.07369307197428299 1101 46-47 -0.12504446613476716 1101 48-49 -0.10626826306332049 1101 50-51 -0.17191101961537925 1101 52-53 0.022340715593095695 1101 54-55 -0.24071792815793458 1101 56-57 -0.27099882117158103 1101 58-59 -0.29841455006103246 1101 60-61 -0.13090004516202924 1101 62-63 -0.11671684512191405 1101 64-65 -0.06101136134845575 1101 66-67 -0.07995104185297208 1101 68-69 0.05933824284996092 1101 70-71 -0.14431220400505396 1101 72-73 0.04342288926765292 1101 74-75 -0.20293788503368404 1101 76-77 0.010819019661717277 1101 78-79 -0.18692697355301746 1101 80-81 -0.22429020566451996 1101 82-83 -0.22833267653806288 1101 84-85 -0.22859543038600805 1101 86-87 -0.21804685022113546 1101 88-89 -0.20698993572093727 1101 90-91 -0.23948911403113726 1101 92-93 -0.2411644723723363 1101 94-95 -0.023114361848868725 1101 96-97 -0.23663380230459552 1101 98-99 -0.029936629396125625 1101 100-101 0.6830021539268643 1102 1 -0.21277448490653939 1102 2 -0.05971661572441178 1102 3 -0.03737899695951796 1102 4 -0.04998542969147479 1102 5 -0.1439975735230874 1102 6 -0.08665356233833421 1102 7 -0.16958219840905286 1102 8 -0.2234858208729591 1102 9 -0.3303252191716979 1102 10-11 -0.2896789344782249 1102 12-13 -0.30126359201661757 1102 14-15 -0.2565653770361891 1102 16-17 -0.14894636611274592 1102 18-19 -0.1825422209715697 1102 20-21 -0.1769525121011597 1102 22-23 -0.40769783143653626 1102 24-25 -0.33737128926968296 1102 26-27 -0.3260777162952806 1102 28-29 -0.3309752517395097 1102 30-31 -0.21875427748437914 1102 32-33 -0.2916038565515393 1102 34-35 -0.2568436659173585 1102 36-37 -0.34419494961202446 1102 38-39 -0.2711476015789813 1102 40-41 -0.18760854297145357 1102 42-43 -0.14271820050316109 1102 44-45 -0.09601042854818331 1102 46-47 -0.007375317754409139 1102 48-49 0.21408505459603333 1102 50-51 0.16807831336963375 1102 52-53 0.1416915515387629 1102 54-55 0.1157835086236716 1102 56-57 0.11940304229062093 1102 58-59 0.17942418208804867 1102 60-61 0.10010613735277474 1102 62-63 -0.1059028865708811 1102 64-65 -0.03338596949892292 1102 66-67 0.056597634566394106 1102 68-69 -7.018128797469103E-4 1102 70-71 -0.10727153873163076 1102 72-73 -0.04026963946798645 1102 74-75 -0.2826411678619607 1102 76-77 -0.21576252857931166 1102 78-79 -0.1269957649149127 1102 80-81 -0.17766011162063577 1102 82-83 -0.4352455553259382 1102 84-85 -0.43568246452045756 1102 86-87 -0.4806785552019832 1102 88-89 -0.47214689322703407 1102 90-91 -0.41372065356440046 1102 92-93 -0.23903106937686402 1102 94-95 -0.03117993133685104 1102 96-97 -0.22094349404963154 1102 98-99 -0.1909214778809698 1102 100-101 0.45763969311181896 1103 1 -0.05274438091091582 1103 2 0.04352640562206034 1103 3 -0.00644029964151116 1103 4 -0.011233377146318446 1103 5 -0.036307371005300126 1103 6 -0.11492395095355334 1103 7 -0.04429484208721135 1103 8 -0.1783982456129678 1103 9 -0.22842319399380528 1103 10-11 -0.1787730778827168 1103 12-13 -0.1480205706701483 1103 14-15 -0.22996439181451223 1103 16-17 -0.3936165905243456 1103 18-19 -0.26287199655996574 1103 20-21 -0.24432388593804433 1103 22-23 -0.16323286154053562 1103 24-25 -0.09331682840488043 1103 26-27 -0.14416748093677256 1103 28-29 -0.18419090581723196 1103 30-31 -0.12956434863927768 1103 32-33 -0.09252750187173575 1103 34-35 -0.04413841140176089 1103 36-37 -0.2040197990920518 1103 38-39 -0.10612297103710944 1103 40-41 -0.021509747131283063 1103 42-43 -0.01567249716435981 1103 44-45 -0.2043642873330711 1103 46-47 -0.11285561332091731 1103 48-49 -0.12938785180791257 1103 50-51 -0.003959179788544986 1103 52-53 0.09536560736798805 1103 54-55 -0.05158102339603232 1103 56-57 0.09022953380677023 1103 58-59 0.10402051487402986 1103 60-61 0.12751445700246933 1103 62-63 0.3591354276053593 1103 64-65 0.2843890715519777 1103 66-67 0.2700760144240846 1103 68-69 0.03163261514433202 1103 70-71 0.16053086958802254 1103 72-73 0.06217288926765008 1103 74-75 0.055057244836447694 1103 76-77 0.3519851452028462 1103 78-79 0.178224541598496 1103 80-81 0.1331773268030112 1103 82-83 0.21475444467406035 1103 84-85 0.19571463454905924 1103 86-87 0.13924481644552955 1103 88-89 0.3426177482617483 1103 90-91 0.15615482536280467 1103 92-93 0.27799137178351074 1103 94-95 0.4309711359866313 1103 96-97 0.43283318903739243 1103 98-99 0.6501499506904551 1103 100-101 0.9139410067407177 1104 1 0.17232875414514837 1104 2 0.12683942366204093 1104 3 0.15433899501150705 1104 4 0.05585584762208384 1104 5 0.07522358950176766 1104 6 0.08804208187596174 1104 7 0.010540197730762202 1104 8 0.08080458227586718 1104 9 0.15227321426232976 1104 10-11 0.1433055829331451 1104 12-13 0.1294619581775649 1104 14-15 0.19567757145542686 1104 16-17 -0.012808448154771668 1104 18-19 0.12813442307272993 1104 20-21 0.15499961479330437 1104 22-23 0.007986462366503133 1104 24-25 0.16986982851044985 1104 26-27 -0.1605863010245301 1104 28-29 0.0684582084359775 1104 30-31 0.07421023286568129 1104 32-33 0.0620454706619924 1104 34-35 0.027511596724366427 1104 36-37 0.08591600458095883 1104 38-39 0.112428954852696 1104 40-41 0.13544011472472306 1104 42-43 0.07243289857755286 1104 44-45 -0.04918998211611125 1104 46-47 0.1046053813158494 1104 48-49 0.11414782184721872 1104 50-51 0.22711712445328658 1104 52-53 0.17621844338294324 1104 54-55 0.037314637257310324 1104 56-57 -0.14409518584218262 1104 58-59 -0.016004269783856273 1104 60-61 0.08157345261437143 1104 62-63 0.17867467675211657 1104 64-65 0.21760457618385942 1104 66-67 0.14469408690905539 1104 68-69 0.13592320510044686 1104 70-71 0.2962614075369814 1104 72-73 0.4289106594610459 1104 74-75 0.11833532057186602 1104 76-77 0.26792452436747993 1104 78-79 0.04828995684309234 1104 80-81 0.1473614646219623 1104 82-83 0.20753032635779078 1104 84-85 0.08134969061926611 1104 86-87 0.04786256388580057 1104 88-89 0.4004878929066358 1104 90-91 0.01180044538555336 1104 92-93 0.11560716895561995 1104 94-95 0.2760020152325282 1104 96-97 0.2260966377615965 1104 98-99 0.1655916051679469 1104 100-101 0.5610095099099048 1105 1 -0.11700391396087895 1105 2 -0.01822280894086248 1105 3 0.019573068189451703 1105 4 -0.07432707516886694 1105 5 -0.10233028728695359 1105 6 -0.13002590376572698 1105 7 -0.15373856783106987 1105 8 -0.09302431596348981 1105 9 -0.1317420506438438 1105 10-11 -0.06356023983675385 1105 12-13 -0.11331657264144468 1105 14-15 -0.20332267432545592 1105 16-17 -0.2505985100709509 1105 18-19 -0.05598710159292608 1105 20-21 -0.11843603403143987 1105 22-23 -0.1905529810501747 1105 24-25 -0.24160195746296154 1105 26-27 -0.2452901939288239 1105 28-29 -0.15859181754026253 1105 30-31 -0.1580048710319346 1105 32-33 -0.06128943743510007 1105 34-35 -0.06056405669221476 1105 36-37 -0.2149558552739066 1105 38-39 -0.10108693333612706 1105 40-41 -0.28210775735735893 1105 42-43 -0.274613389174462 1105 44-45 -0.23422398671088018 1105 46-47 -0.22863596815366805 1105 48-49 0.0821241915605384 1105 50-51 -0.03828354989881433 1105 52-53 -0.03544893707850605 1105 54-55 -0.09759993548868096 1105 56-57 -0.1289104896023332 1105 58-59 -0.04524410041580751 1105 60-61 -0.20493641467867008 1105 62-63 0.04040968375024789 1105 64-65 -0.027594234060046574 1105 66-67 0.03934386386658417 1105 68-69 0.29613701975222995 1105 70-71 0.22408350927261012 1105 72-73 0.09316146192827546 1105 74-75 0.37128653085874674 1105 76-77 0.329116605807787 1105 78-79 0.09081078566921619 1105 80-81 0.15232197043019013 1105 82-83 0.11822576852054212 1105 84-85 0.2439381606994928 1105 86-87 0.01146344516480724 1105 88-89 0.16529839878844754 1105 90-91 0.25344352738953546 1105 92-93 0.5776494756460124 1105 94-95 0.561998980500892 1105 96-97 0.42380528292022746 1105 98-99 0.558701204309628 1105 100-101 1.3419834203426397 1106 1 -0.03431949978111959 1106 2 0.13243954166634353 1106 3 0.06846034616077645 1106 4 -0.053771167225093564 1106 5 -0.09863438825035331 1106 6 -0.15282402901756598 1106 7 -0.07588947696054049 1106 8 -0.06210947973404046 1106 9 -0.055655115078188544 1106 10-11 -0.08532690656769404 1106 12-13 -0.15536923293684168 1106 14-15 -0.09176802533946216 1106 16-17 0.02342531565690109 1106 18-19 0.04486255031392261 1106 20-21 0.11803595367589281 1106 22-23 -0.020919505497673185 1106 24-25 -0.07912603286162323 1106 26-27 -0.1761506616904427 1106 28-29 -0.1750112876212242 1106 30-31 -0.16932199536413606 1106 32-33 -0.30480040656266993 1106 34-35 -0.2437134947883095 1106 36-37 -0.21748051870031304 1106 38-39 0.04519702044681395 1106 40-41 -0.022983205367033577 1106 42-43 -0.30269701636101587 1106 44-45 -0.140120266326754 1106 46-47 0.029008874650131133 1106 48-49 -0.04040729684374611 1106 50-51 -0.0014797603009242266 1106 52-53 0.06585474937352842 1106 54-55 -0.09247059830200044 1106 56-57 -0.1022053537100831 1106 58-59 0.2791438205301233 1106 60-61 0.3485615792790995 1106 62-63 0.15850204459492545 1106 64-65 0.21541064560634027 1106 66-67 0.22995643455040948 1106 68-69 0.174092341210617 1106 70-71 0.19058906276097076 1106 72-73 0.2617692628394295 1106 74-75 0.07737290731604674 1106 76-77 0.4737241984992764 1106 78-79 0.5571428227708779 1106 80-81 0.39071227819042065 1106 82-83 0.09878325739139981 1106 84-85 0.3061707767674946 1106 86-87 0.18183050944105617 1106 88-89 0.23865245126366474 1106 90-91 0.21007310653518374 1106 92-93 0.5738504307573216 1106 94-95 0.42989420745068685 1106 96-97 0.6538359567614727 1106 98-99 0.5120715673251865 1106 100-101 0.7936967018652652 1107 1 0.1321479970929147 1107 2 -0.025856610742380326 1107 3 0.045752682814629964 1107 4 0.07063305363652006 1107 5 0.045183119927912685 1107 6 -0.22514804058940285 1107 7 -0.0987840061739007 1107 8 0.042613843433173315 1107 9 0.10301938303700808 1107 10-11 0.1467097468261187 1107 12-13 0.020393110591832908 1107 14-15 -9.027671646535396E-4 1107 16-17 0.08039748879158992 1107 18-19 0.06644209749870811 1107 20-21 0.12367884146870978 1107 22-23 0.09883959902263229 1107 24-25 0.06778629705583228 1107 26-27 0.1708811701025965 1107 28-29 -0.05393143360729624 1107 30-31 0.04054106194601559 1107 32-33 0.08887637546867211 1107 34-35 0.03204285057671541 1107 36-37 0.11511996207557473 1107 38-39 0.07875097854272184 1107 40-41 0.15077206032854207 1107 42-43 0.11833715932981903 1107 44-45 0.04764993941025608 1107 46-47 -0.10671341960131997 1107 48-49 0.1033594576421848 1107 50-51 0.1713596319466717 1107 52-53 0.21479838047723376 1107 54-55 0.2760358128121325 1107 56-57 -0.034722552290823216 1107 58-59 0.15773752799506724 1107 60-61 0.12114927935245845 1107 62-63 -0.09171596558349648 1107 64-65 0.15219461482191576 1107 66-67 0.16647510037426372 1107 68-69 0.3928592110458524 1107 70-71 0.26408386973545106 1107 72-73 0.3627275848300826 1107 74-75 0.3241269779928686 1107 76-77 0.22328471766341096 1107 78-79 0.10795880372440081 1107 80-81 0.28635807278561387 1107 82-83 0.0038669317741621967 1107 84-85 0.45657819049775483 1107 86-87 0.3767835161359301 1107 88-89 0.07825463459522553 1107 90-91 0.20748351473329052 1107 92-93 0.374055060416854 1107 94-95 0.5086985427388058 1107 96-97 0.5328553031461958 1107 98-99 0.7029123714482317 1107 100-101 1.5458571942683612 1108 1 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0.4404961523018329 2316 56-57 1.0197861840530749 2316 58-59 0.8652479368773172 2316 60-61 0.47845862777423065 2316 62-63 0.633560714961682 2316 64-65 0.12178644429417318 2316 66-67 0.34203824759321577 2316 68-69 0.37908745915090236 2316 70-71 0.4937821011151087 2316 72-73 -0.6279133176289058 2316 74-75 0.3950408244751955 2316 76-77 0.04094245226360016 2316 78-79 -0.06904557334403094 2316 80-81 0.1226272447012029 2316 82-83 0.5819958239844034 2316 84-85 0.706079987586822 2316 86-87 0.16539424173288353 2316 88-89 0.5125274362748833 2316 90-91 0.2907812621444137 2316 92-93 0.17380417966528228 2316 94-95 -0.3831914255897253 2316 96-97 -0.037035448072622046 2316 98-99 0.26166472901557825 2316 100-101 0.7794787735715865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 3.0 5 8.0 6 20.0 7 54.0 8 118.0 9 210.0 10 455.0 11 776.0 12 1245.0 13 1922.0 14 2410.0 15 3690.0 16 5380.0 17 8527.0 18 7793.0 19 4050.0 20 3101.0 21 3034.0 22 3096.0 23 3334.0 24 3728.0 25 4126.0 26 5072.0 27 6099.0 28 7748.0 29 9692.0 30 12162.0 31 16049.0 32 20779.0 33 28666.0 34 40719.0 35 60986.0 36 98580.0 37 162889.0 38 145885.0 39 15035.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21177785799961 20.164079512768858 15.475469588811382 21.148673040420153 2 15.528537597244284 21.13364870447629 40.336812889486275 23.001000808793155 3 19.160251598675675 25.814466341985675 30.812400719185856 24.212881340152798 4 12.617464113440512 17.721152559335742 37.94199963924334 31.7193836879804 5 13.031201858524613 38.508525268864915 34.21570581312634 14.244567059484133 6 30.49877517295954 37.64961218659265 17.56855831165884 14.283054328788964 7 27.362083311513373 34.29370246885565 20.816822897574202 17.52739132205678 8 26.458737002577664 35.40957517994193 19.63156271638126 18.500125101099144 9 26.67868219084027 16.754964768039287 20.59222278469228 35.97413025642816 10-11 24.40753312817708 27.84977965461889 27.13393900735115 20.60874820985288 12-13 25.711006304862043 25.239887344596934 28.221240431418988 20.827865919122033 14-15 22.71305008117025 26.11936390455077 27.131591809660133 24.03599420461885 16-17 21.205218170498252 29.686927226072235 27.644579049348017 21.463275554081495 18-19 21.023239711161928 27.56624539598862 31.035400701729888 20.375114191119568 20-21 21.9809177183887 26.636933335660796 31.31724184078994 20.064907105160565 22-23 21.678542932391476 26.641408539419338 31.110336897061615 20.569711631127575 24-25 21.351759250840985 26.640458223474862 31.419510864624055 20.588271661060094 26-27 21.271632828512786 27.05621880632318 31.178184537100744 20.49396382806329 28-29 20.98185872924548 27.158662836834758 31.415580646498707 20.443897787421054 30-31 21.33681776624382 26.994487506882436 31.17900728574265 20.489687441131096 32-33 21.12921197944851 27.019219020021996 30.936410820372277 20.91515818015722 34-35 21.307699245320347 27.018928087233284 31.060711854347407 20.612660813098955 36-37 21.506073958131804 27.030968385742526 30.817609605450926 20.645348050674748 38-39 21.214893928076055 27.125493410644943 31.047321599366924 20.61229106191208 40-41 21.50962773532565 27.17367745013754 30.795515542424734 20.521179272112082 42-43 21.378721881301338 27.372358348100107 30.493748490588544 20.75517128001001 44-45 21.479266614492566 27.38274647170234 30.271135601258 20.866851312547094 46-47 21.588813052408632 27.597666137168993 29.79588155544306 21.017639254979315 48-49 21.773555373237674 27.8216843844735 29.3896666492107 21.015093593078127 50-51 21.805776179586992 28.10738038298392 29.065785722182465 21.021057715246624 52-53 21.91902176759125 28.298377758770172 28.781544387615572 21.001056086023006 54-55 21.703513304356427 28.196333083131137 28.833039491216738 21.2671141212957 56-57 21.73289751601585 28.43751636496937 28.68539110094786 21.144195018066927 58-59 21.846724969597524 28.032828855877717 28.931011107813877 21.18943506671089 60-61 21.766572986308702 28.00759043645737 29.16615753428643 21.0596790429475 62-63 21.67812941854173 27.999517051570745 29.2596196926586 21.062733837228922 64-65 21.681329679217505 27.947658281983696 29.207688189874375 21.16332384892442 66-67 21.438619000238564 28.244773392450877 28.968395971162742 21.348211636147816 68-69 21.58663105649333 28.086069556211125 28.91326420770274 21.414035179592812 70-71 21.44865618144896 28.250664781422195 28.822056778443045 21.478622258685796 72-73 21.500878617021897 28.14716544183963 28.721103100761663 21.630852840376814 74-75 21.565974828495122 28.300196088699586 28.434534303885115 21.699294778920176 76-77 21.60314149225246 27.96962370753109 28.7601608276456 21.667073972570858 78-79 21.521316645428573 28.143674248375138 28.570981781788774 21.764027324407515 80-81 21.46531208360245 28.477810556205306 28.323907110979224 21.732970249213025 82-83 21.288938767880612 28.243130506377632 28.464749364127712 22.003181361614043 84-85 21.443684380875492 28.14839059266775 28.484927830327628 21.92299719612913 86-87 21.45389297164569 28.13232786961556 28.312342532628108 22.101436626110633 88-89 21.332792198346336 28.023519006639084 28.292995502179085 22.350693292835487 90-91 21.18509315685878 28.1078836001158 28.439573516596862 22.26744972642856 92-93 21.198561586444463 27.8966111162834 28.38881098128666 22.516016315985475 94-95 20.995455629840396 27.789827244110064 28.439552894490316 22.775164231559224 96-97 21.153577600502732 27.85179592810469 28.199897009792796 22.79472946159978 98-99 20.963541003733397 27.8633080391334 28.220646496766644 22.95250446036656 100-101 20.683351712780166 28.2322851714235 28.076763044800266 23.00760007099607 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 202.0 1 137.0 2 103.5 3 140.5 4 127.0 5 139.0 6 242.0 7 519.5 8 840.5 9 1088.0 10 1143.5 11 1091.5 12 1178.0 13 1294.0 14 1410.0 15 1477.5 16 1532.5 17 1569.0 18 1574.5 19 1663.5 20 1793.0 21 2035.0 22 2308.0 23 2580.0 24 3007.0 25 3640.0 26 4464.5 27 5591.0 28 6714.5 29 8019.0 30 9592.0 31 10816.0 32 12100.5 33 13627.5 34 15579.0 35 17120.0 36 18789.5 37 20730.5 38 21738.0 39 22410.5 40 23087.0 41 23802.5 42 24320.5 43 24870.5 44 25777.0 45 26380.5 46 26113.5 47 26029.0 48 27452.5 49 27453.5 50 25449.0 51 23770.0 52 22024.0 53 20582.5 54 19340.0 55 17352.5 56 14952.5 57 13002.0 58 11729.0 59 10366.0 60 8669.5 61 6896.0 62 5197.0 63 3765.0 64 2598.0 65 1832.0 66 1323.0 67 1039.0 68 740.5 69 454.0 70 294.0 71 192.0 72 138.5 73 116.5 74 79.0 75 68.0 76 58.0 77 36.0 78 22.0 79 9.0 80 8.5 81 8.5 82 6.0 83 6.0 84 2.5 85 1.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06691454140264516 2 0.0 3 0.0 4 0.0 5 0.0024729287040107994 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.273319717678823E-5 12-13 0.005527722985435904 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012364643520053997 24-25 9.455315632982468E-4 26-27 7.273319717678822E-4 28-29 0.004873124210844811 30-31 8.000651689446705E-4 32-33 0.0 34-35 0.0 36-37 1.4546639435357645E-4 38-39 0.001381930746358976 40-41 4.363991830607293E-4 42-43 0.013091975491821879 44-45 2.909327887071529E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.636659858839411E-4 84-85 2.1819959153036465E-4 86-87 0.0 88-89 0.0 90-91 0.008000651689446704 92-93 0.002909327887071529 94-95 0.0 96-97 0.0 98-99 7.273319717678823E-5 100-101 0.011928244336993268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 687444.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.94818668205863 #Duplication Level Percentage of deduplicated Percentage of total 1 82.07888514432469 53.3087475500885 2 10.741049740082262 13.952234073602806 3 3.040551387539832 5.924348974029884 4 1.3470448929783938 3.4995249271309774 5 0.703791588303892 2.2854993731211866 6 0.45364744683308295 1.7678147438852605 7 0.2999841038156558 1.363839650338849 8 0.22914445077503806 1.1906013252875975 9 0.16424187771891863 0.9600490921590158 >10 0.8774210137211336 10.121967973227793 >50 0.04620665915378688 2.032650908218634 >100 0.01690471383141807 1.8025091154043986 >500 4.507923687640767E-4 0.22397782394822557 >1k 6.761885531461152E-4 1.5662344695569055 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4439 0.6457253245355258 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3797 0.5523358993605297 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2463 0.3582837292928588 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 957 0.13921133939637265 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 1.4546639435357643E-4 0.0 0.0 0.0 0.0 9 1.4546639435357643E-4 0.0 0.0 0.0 0.0 10-11 1.4546639435357643E-4 0.0 0.0 0.0 0.0 12-13 2.9093278870715285E-4 0.0 0.0 0.0 0.0 14-15 2.9093278870715285E-4 0.0 0.0 0.0 0.0 16-17 2.9093278870715285E-4 0.0 0.0 0.0 0.0 18-19 4.363991830607293E-4 0.0 0.0 0.0 0.0 20-21 4.363991830607293E-4 0.0 0.0 0.0 0.0 22-23 4.363991830607293E-4 0.0 0.0 2.1819959153036465E-4 0.0 24-25 4.363991830607293E-4 0.0 0.0 8.727983661214586E-4 0.0 26-27 4.363991830607293E-4 0.0 0.0 0.0016001303378893408 0.0 28-29 5.818655774143057E-4 0.0 0.0 0.00327299387295547 0.0 30-31 5.818655774143057E-4 0.0 0.0 0.007491519309209186 0.0 32-33 5.818655774143057E-4 0.0 0.0 0.014328439843827279 0.0 34-35 5.818655774143057E-4 0.0 0.0 0.01963796323773282 0.0 36-37 7.273319717678822E-4 0.0 0.0 0.027711348124356314 0.0 38-39 7.273319717678822E-4 0.0 0.0 0.03833039491216739 0.0 40-41 7.273319717678822E-4 0.0 0.0 0.06007762086802707 0.0 42-43 8.000651689446704E-4 0.0 0.0 0.08102478165494208 0.0 44-45 8.727983661214586E-4 0.0 0.0 0.10560860230069649 0.0 46-47 8.727983661214586E-4 0.0 0.0 0.1529579136627856 0.0 48-49 8.727983661214586E-4 0.0 0.0 0.18692431674434573 0.0 50-51 8.727983661214586E-4 0.0 0.0 0.2296914366842972 0.0 52-53 8.727983661214586E-4 0.0 0.0 0.280241008722165 0.0 54-55 8.727983661214586E-4 0.0 0.0 0.3500648780118817 0.0 56-57 8.727983661214586E-4 0.0 0.0 0.5039683232379656 0.0 58-59 8.727983661214586E-4 0.0 0.0 0.6490710516056581 0.0 60-61 8.727983661214586E-4 0.0 0.0 0.7786088757775179 0.0 62-63 8.727983661214586E-4 0.0 0.0 0.938621909566452 0.0 64-65 8.727983661214586E-4 0.0 0.0 1.0990713425384468 0.0 66-67 8.727983661214586E-4 0.0 0.0 1.3275263148707386 0.0 68-69 8.727983661214586E-4 0.0 0.0 1.6013668022413463 0.0 70-71 8.727983661214586E-4 0.0 0.0 1.7864000558590956 0.0 72-73 8.727983661214586E-4 0.0 0.0 2.011582034318432 0.0 74-75 8.727983661214586E-4 0.0 0.0 2.3551009245844026 0.0 76-77 8.727983661214586E-4 0.0 0.0 2.773607741139642 0.0 78-79 8.727983661214586E-4 0.0 0.0 3.17720425227364 0.0 80-81 8.727983661214586E-4 0.0 0.0 3.4986849837950436 0.0 82-83 8.727983661214586E-4 0.0 0.0 3.9549403296850363 0.0 84-85 8.727983661214586E-4 0.0 0.0 4.540078900972297 0.0 86-87 8.727983661214586E-4 0.0 0.0 5.071685839137443 0.0 88-89 8.727983661214586E-4 0.0 0.0 5.705337452941621 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2590 0.0 47.518772 1 GTATCAA 4340 0.0 35.803318 1 TATCAAC 5460 0.0 28.446627 2 ATCAACG 5505 0.0 28.12781 3 TCAACGC 5550 0.0 27.899748 4 CAACGCA 5680 0.0 27.34482 5 AACGCAG 5780 0.0 26.871725 6 ATAGACG 90 8.9460344E-4 26.387796 3 TAAGGTG 255 1.8189894E-11 24.214685 5 ACGCAGA 6605 0.0 23.446802 7 GAGTACT 4055 0.0 23.254578 12-13 CGCAGAG 6625 0.0 23.160902 8 TACCTGG 520 0.0 22.835594 2 GTACCTG 595 0.0 22.361776 1 GCAGAGT 6925 0.0 21.951744 9 GTACTTT 4280 0.0 21.9179 14-15 AGTACTT 4175 0.0 21.846588 12-13 GTACTAC 135 3.783692E-4 21.119455 1 CTTAGAC 190 8.246274E-6 19.999174 3 GTCTAGG 240 1.4052421E-7 19.79949 1 >>END_MODULE