##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937295_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1035 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.156521739130437 28.0 16.0 31.0 2.0 33.0 2 23.004830917874397 30.0 16.0 31.0 2.0 33.0 3 22.58743961352657 28.0 16.0 31.0 2.0 33.0 4 25.61256038647343 33.0 16.0 35.0 2.0 37.0 5 25.683091787439615 32.0 19.0 35.0 2.0 37.0 6 25.45024154589372 33.0 15.0 35.0 2.0 37.0 7 24.67729468599034 32.0 10.0 35.0 2.0 37.0 8 24.768115942028984 32.0 10.0 35.0 2.0 37.0 9 25.6743961352657 32.0 11.0 37.0 2.0 39.0 10-11 25.125603864734302 32.0 10.0 37.0 2.0 39.0 12-13 24.5231884057971 32.0 9.0 37.0 2.0 39.0 14-15 24.43913043478261 32.0 5.0 37.0 2.0 39.5 16-17 24.327053140096616 32.0 2.0 37.0 2.0 40.0 18-19 24.430917874396137 32.0 2.0 38.0 2.0 40.0 20-21 24.278260869565216 32.0 2.0 38.0 2.0 40.0 22-23 23.913526570048308 31.5 2.0 38.0 2.0 40.0 24-25 23.860386473429955 31.5 2.0 38.0 2.0 40.0 26-27 23.642028985507245 31.5 2.0 38.0 2.0 40.0 28-29 22.746376811594203 30.0 2.0 37.5 2.0 39.5 30-31 22.15942028985507 30.0 2.0 36.5 2.0 39.0 32-33 21.941545893719805 30.0 2.0 35.5 2.0 39.0 34-35 21.542995169082126 29.0 2.0 36.0 2.0 39.0 36-37 21.22705314009662 27.0 2.0 35.5 2.0 39.0 38-39 21.089371980676326 28.5 2.0 35.5 2.0 39.0 40-41 20.458454106280193 26.0 2.0 35.5 2.0 39.0 42-43 19.870531400966186 25.0 2.0 35.0 2.0 39.0 44-45 19.305797101449276 23.5 2.0 35.0 2.0 39.0 46-47 18.48454106280193 19.0 2.0 35.0 2.0 38.5 48-49 18.09951690821256 14.0 2.0 35.0 2.0 39.0 50-51 17.339613526570048 9.0 2.0 34.0 2.0 38.0 52-53 17.269565217391303 5.5 2.0 34.5 2.0 38.0 54-55 17.35217391304348 2.0 2.0 35.0 2.0 39.0 56-57 16.97584541062802 2.0 2.0 35.0 2.0 38.5 58-59 16.433333333333334 2.0 2.0 34.0 2.0 38.0 60-61 15.815458937198068 2.0 2.0 33.0 2.0 38.0 62-63 15.317391304347826 2.0 2.0 33.0 2.0 38.0 64-65 15.019806763285025 2.0 2.0 32.5 2.0 38.0 66-67 14.56038647342995 2.0 2.0 32.0 2.0 37.0 68-69 13.956038647342996 2.0 2.0 31.0 2.0 36.5 70-71 13.647826086956522 2.0 2.0 31.0 2.0 36.0 72-73 12.944444444444445 2.0 2.0 30.0 2.0 35.5 74-75 12.370048309178745 2.0 2.0 29.5 2.0 35.0 76-77 11.87632850241546 2.0 2.0 29.0 2.0 35.0 78-79 11.309178743961352 2.0 2.0 27.0 2.0 34.5 80-81 10.74927536231884 2.0 2.0 26.0 2.0 34.0 82-83 9.945893719806763 2.0 2.0 21.5 2.0 34.0 84-85 9.614492753623189 2.0 2.0 20.0 2.0 33.5 86-87 8.970531400966184 2.0 2.0 6.5 2.0 33.0 88-89 8.383091787439614 2.0 2.0 2.0 2.0 32.5 90-91 7.9628019323671495 2.0 2.0 2.0 2.0 31.5 92-93 7.464734299516908 2.0 2.0 2.0 2.0 31.0 94-95 6.877777777777778 2.0 2.0 2.0 2.0 30.0 96-97 6.183091787439613 2.0 2.0 2.0 2.0 28.5 98-99 5.6980676328502415 2.0 2.0 2.0 2.0 28.0 100-101 4.818357487922706 2.0 2.0 2.0 2.0 18.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1102 1 -2.2984496124031004 1102 2 0.7519379844961236 1102 3 -3.0484496124031004 1102 4 0.5581395348837219 1102 5 -4.891472868217058 1102 6 -2.9089147286821735 1102 7 4.556201550387595 1102 8 -3.5794573643410814 1102 9 0.19961240310077955 1102 10-11 -8.600775193798452 1102 12-13 0.04360465116279144 1102 14-15 0.1550387596899192 1102 16-17 -4.41085271317829 1102 18-19 9.63953488372093 1102 20-21 12.466085271317827 1102 22-23 6.102713178294572 1102 24-25 0.6424418604651159 1102 26-27 -8.03585271317829 1102 28-29 -4.445736434108525 1102 30-31 -3.3488372093023244 1102 32-33 -5.652131782945734 1102 34-35 -4.684108527131784 1102 36-37 -4.609496124031008 1102 38-39 -3.058139534883722 1102 40-41 -1.117248062015502 1102 42-43 -0.05620155038759478 1102 44-45 0.9147286821705443 1102 46-47 -0.3498062015503862 1102 48-49 0.9312015503875948 1102 50-51 1.0939922480620154 1102 52-53 -1.959302325581394 1102 54-55 -6.41763565891473 1102 56-57 -0.23352713178294593 1102 58-59 -0.4476744186046524 1102 60-61 2.3527131782945734 1102 62-63 -4.5445736434108515 1102 64-65 -14.006782945736433 1102 66-67 -14.032945736434108 1102 68-69 -13.021317829457363 1102 70-71 -13.437015503875967 1102 72-73 -12.301356589147288 1102 74-75 -11.17732558139535 1102 76-77 -10.623062015503878 1102 78-79 -10.48546511627907 1102 80-81 -10.017441860465116 1102 82-83 -7.468023255813954 1102 84-85 -6.922480620155039 1102 86-87 -6.39922480620155 1102 88-89 -5.840116279069767 1102 90-91 -5.6443798449612395 1102 92-93 -5.291666666666667 1102 94-95 -4.531976744186046 1102 96-97 -3.770348837209302 1102 98-99 -3.950581395348837 1102 100-101 -2.6889534883720927 1104 1 -12.7984496124031 1104 2 -12.748062015503876 1104 3 -13.0484496124031 1104 4 -7.441860465116278 1104 5 5.608527131782942 1104 6 -16.408914728682173 1104 7 6.556201550387595 1104 8 -14.079457364341081 1104 9 3.1996124031007795 1104 10-11 -23.35077519379845 1104 12-13 -22.95639534883721 1104 14-15 -22.84496124031008 1104 16-17 -23.41085271317829 1104 18-19 -23.11046511627907 1104 20-21 -22.033914728682173 1104 22-23 -21.39728682170543 1104 24-25 -22.857558139534884 1104 26-27 -23.03585271317829 1104 28-29 -22.195736434108525 1104 30-31 -20.848837209302324 1104 32-33 -21.402131782945734 1104 34-35 -21.184108527131784 1104 36-37 -20.359496124031008 1104 38-39 -20.558139534883722 1104 40-41 -19.867248062015502 1104 42-43 -18.306201550387595 1104 44-45 -18.335271317829456 1104 46-47 -18.099806201550386 1104 48-49 -17.818798449612405 1104 50-51 -16.656007751937985 1104 52-53 -16.459302325581394 1104 54-55 -16.66763565891473 1104 56-57 -16.483527131782946 1104 58-59 -15.197674418604652 1104 60-61 -13.647286821705427 1104 62-63 -13.794573643410851 1104 64-65 -14.006782945736433 1104 66-67 -14.032945736434108 1104 68-69 -13.021317829457363 1104 70-71 -13.437015503875967 1104 72-73 -12.301356589147288 1104 74-75 -11.17732558139535 1104 76-77 -10.623062015503878 1104 78-79 -10.48546511627907 1104 80-81 -10.017441860465116 1104 82-83 -7.468023255813954 1104 84-85 -6.922480620155039 1104 86-87 -6.39922480620155 1104 88-89 -5.840116279069767 1104 90-91 -5.6443798449612395 1104 92-93 -5.291666666666667 1104 94-95 -4.531976744186046 1104 96-97 -3.770348837209302 1104 98-99 -3.950581395348837 1104 100-101 -2.6889534883720927 1105 1 -6.7984496124031 1105 2 -6.748062015503876 1105 3 -7.0484496124031 1105 4 5.558139534883722 1105 5 -0.3914728682170576 1105 6 6.5910852713178265 1105 7 9.556201550387595 1105 8 9.920542635658919 1105 9 10.19961240310078 1105 10-11 -3.8507751937984516 1105 12-13 -7.956395348837209 1105 14-15 -6.344961240310081 1105 16-17 5.58914728682171 1105 18-19 4.3895348837209305 1105 20-21 6.4660852713178265 1105 22-23 6.102713178294572 1105 24-25 3.142441860465116 1105 26-27 -6.53585271317829 1105 28-29 5.804263565891475 1105 30-31 -5.348837209302324 1105 32-33 -6.902131782945734 1105 34-35 11.815891472868216 1105 36-37 10.640503875968992 1105 38-39 -4.558139534883722 1105 40-41 5.632751937984498 1105 42-43 9.693798449612405 1105 44-45 9.164728682170544 1105 46-47 2.400193798449614 1105 48-49 -3.318798449612405 1105 50-51 5.3439922480620154 1105 52-53 10.540697674418606 1105 54-55 17.33236434108527 1105 56-57 14.516472868217054 1105 58-59 12.302325581395348 1105 60-61 -8.647286821705427 1105 62-63 -6.7945736434108515 1105 64-65 5.993217054263567 1105 66-67 11.467054263565892 1105 68-69 14.978682170542637 1105 70-71 17.06298449612403 1105 72-73 -12.301356589147288 1105 74-75 -11.17732558139535 1105 76-77 -10.623062015503878 1105 78-79 -10.48546511627907 1105 80-81 -10.017441860465116 1105 82-83 -7.468023255813954 1105 84-85 -6.922480620155039 1105 86-87 -6.39922480620155 1105 88-89 -5.840116279069767 1105 90-91 -5.6443798449612395 1105 92-93 -5.291666666666667 1105 94-95 -4.531976744186046 1105 96-97 -3.770348837209302 1105 98-99 -3.950581395348837 1105 100-101 -2.6889534883720927 1106 1 10.2015503875969 1106 2 8.251937984496124 1106 3 7.9515503875969 1106 4 10.558139534883722 1106 5 5.608527131782942 1106 6 8.591085271317827 1106 7 10.556201550387595 1106 8 11.920542635658919 1106 9 12.19961240310078 1106 10-11 13.649224806201548 1106 12-13 13.043604651162791 1106 14-15 15.15503875968992 1106 16-17 13.58914728682171 1106 18-19 10.38953488372093 1106 20-21 15.466085271317827 1106 22-23 17.60271317829457 1106 24-25 16.142441860465116 1106 26-27 15.96414728682171 1106 28-29 16.804263565891475 1106 30-31 18.151162790697676 1106 32-33 15.597868217054266 1106 34-35 11.815891472868216 1106 36-37 12.640503875968992 1106 38-39 12.441860465116278 1106 40-41 13.132751937984498 1106 42-43 14.693798449612405 1106 44-45 14.664728682170544 1106 46-47 14.900193798449614 1106 48-49 15.181201550387595 1106 50-51 14.343992248062015 1106 52-53 -16.459302325581394 1106 54-55 -16.66763565891473 1106 56-57 -16.483527131782946 1106 58-59 -15.197674418604652 1106 60-61 -13.647286821705427 1106 62-63 -13.794573643410851 1106 64-65 -14.006782945736433 1106 66-67 -14.032945736434108 1106 68-69 -13.021317829457363 1106 70-71 -13.437015503875967 1106 72-73 -12.301356589147288 1106 74-75 -11.17732558139535 1106 76-77 -10.623062015503878 1106 78-79 -10.48546511627907 1106 80-81 -10.017441860465116 1106 82-83 -7.468023255813954 1106 84-85 -6.922480620155039 1106 86-87 -6.39922480620155 1106 88-89 -5.840116279069767 1106 90-91 -5.6443798449612395 1106 92-93 -5.291666666666667 1106 94-95 -4.531976744186046 1106 96-97 -3.770348837209302 1106 98-99 -3.950581395348837 1106 100-101 -2.6889534883720927 1107 1 10.2015503875969 1107 2 7.251937984496124 1107 3 7.9515503875969 1107 4 8.558139534883722 1107 5 10.608527131782942 1107 6 10.591085271317827 1107 7 11.556201550387595 1107 8 9.920542635658919 1107 9 12.19961240310078 1107 10-11 13.149224806201548 1107 12-13 10.543604651162791 1107 14-15 6.655038759689919 1107 16-17 10.08914728682171 1107 18-19 13.88953488372093 1107 20-21 16.466085271317827 1107 22-23 17.10271317829457 1107 24-25 16.142441860465116 1107 26-27 15.46414728682171 1107 28-29 16.304263565891475 1107 30-31 14.151162790697676 1107 32-33 10.097868217054266 1107 34-35 11.815891472868216 1107 36-37 12.640503875968992 1107 38-39 11.441860465116278 1107 40-41 -3.367248062015502 1107 42-43 -18.306201550387595 1107 44-45 -18.335271317829456 1107 46-47 -18.099806201550386 1107 48-49 -17.818798449612405 1107 50-51 -16.656007751937985 1107 52-53 -16.459302325581394 1107 54-55 -16.66763565891473 1107 56-57 -16.483527131782946 1107 58-59 -15.197674418604652 1107 60-61 -13.647286821705427 1107 62-63 -13.794573643410851 1107 64-65 -14.006782945736433 1107 66-67 -14.032945736434108 1107 68-69 -13.021317829457363 1107 70-71 -13.437015503875967 1107 72-73 -12.301356589147288 1107 74-75 -11.17732558139535 1107 76-77 -10.623062015503878 1107 78-79 -10.48546511627907 1107 80-81 -10.017441860465116 1107 82-83 -7.468023255813954 1107 84-85 -6.922480620155039 1107 86-87 -6.39922480620155 1107 88-89 -5.840116279069767 1107 90-91 -5.6443798449612395 1107 92-93 -5.291666666666667 1107 94-95 -4.531976744186046 1107 96-97 -3.770348837209302 1107 98-99 -3.950581395348837 1107 100-101 -2.6889534883720927 1108 1 8.2015503875969 1108 2 7.251937984496124 1108 3 9.9515503875969 1108 4 8.558139534883722 1108 5 5.608527131782942 1108 6 8.591085271317827 1108 7 9.556201550387595 1108 8 3.9205426356589186 1108 9 5.1996124031007795 1108 10-11 -3.8507751937984516 1108 12-13 4.543604651162791 1108 14-15 9.15503875968992 1108 16-17 11.58914728682171 1108 18-19 8.38953488372093 1108 20-21 8.966085271317827 1108 22-23 0.6027131782945716 1108 24-25 7.642441860465116 1108 26-27 7.96414728682171 1108 28-29 10.804263565891475 1108 30-31 13.151162790697676 1108 32-33 11.597868217054266 1108 34-35 15.315891472868216 1108 36-37 9.640503875968992 1108 38-39 14.441860465116278 1108 40-41 12.632751937984498 1108 42-43 7.693798449612405 1108 44-45 14.664728682170544 1108 46-47 19.400193798449614 1108 48-49 17.181201550387595 1108 50-51 15.843992248062015 1108 52-53 19.040697674418606 1108 54-55 17.33236434108527 1108 56-57 19.516472868217054 1108 58-59 22.802325581395348 1108 60-61 17.35271317829457 1108 62-63 13.205426356589149 1108 64-65 16.493217054263567 1108 66-67 16.967054263565892 1108 68-69 -13.021317829457363 1108 70-71 -13.437015503875967 1108 72-73 -12.301356589147288 1108 74-75 -11.17732558139535 1108 76-77 -10.623062015503878 1108 78-79 -10.48546511627907 1108 80-81 -10.017441860465116 1108 82-83 -7.468023255813954 1108 84-85 -6.922480620155039 1108 86-87 -6.39922480620155 1108 88-89 -5.840116279069767 1108 90-91 -5.6443798449612395 1108 92-93 -5.291666666666667 1108 94-95 -4.531976744186046 1108 96-97 -3.770348837209302 1108 98-99 -3.950581395348837 1108 100-101 -2.6889534883720927 1109 1 -6.7984496124031 1109 2 -6.748062015503876 1109 3 -13.0484496124031 1109 4 1.558139534883722 1109 5 -1.3914728682170576 1109 6 6.5910852713178265 1109 7 9.556201550387595 1109 8 -14.079457364341081 1109 9 5.1996124031007795 1109 10-11 4.149224806201548 1109 12-13 -22.95639534883721 1109 14-15 -22.84496124031008 1109 16-17 -23.41085271317829 1109 18-19 -23.11046511627907 1109 20-21 -22.033914728682173 1109 22-23 -21.39728682170543 1109 24-25 -22.857558139534884 1109 26-27 -23.03585271317829 1109 28-29 -22.195736434108525 1109 30-31 -20.848837209302324 1109 32-33 -21.402131782945734 1109 34-35 -21.184108527131784 1109 36-37 -20.359496124031008 1109 38-39 -20.558139534883722 1109 40-41 -19.867248062015502 1109 42-43 -18.306201550387595 1109 44-45 -18.335271317829456 1109 46-47 -18.099806201550386 1109 48-49 -17.818798449612405 1109 50-51 -16.656007751937985 1109 52-53 -16.459302325581394 1109 54-55 -16.66763565891473 1109 56-57 -16.483527131782946 1109 58-59 -15.197674418604652 1109 60-61 -13.647286821705427 1109 62-63 -13.794573643410851 1109 64-65 -14.006782945736433 1109 66-67 -14.032945736434108 1109 68-69 -13.021317829457363 1109 70-71 -13.437015503875967 1109 72-73 -12.301356589147288 1109 74-75 -11.17732558139535 1109 76-77 -10.623062015503878 1109 78-79 -10.48546511627907 1109 80-81 -10.017441860465116 1109 82-83 -7.468023255813954 1109 84-85 -6.922480620155039 1109 86-87 -6.39922480620155 1109 88-89 -5.840116279069767 1109 90-91 -5.6443798449612395 1109 92-93 -5.291666666666667 1109 94-95 -4.531976744186046 1109 96-97 -3.770348837209302 1109 98-99 -3.950581395348837 1109 100-101 -2.6889534883720927 1110 1 8.2015503875969 1110 2 7.251937984496124 1110 3 6.9515503875969 1110 4 8.558139534883722 1110 5 3.6085271317829424 1110 6 8.591085271317827 1110 7 9.556201550387595 1110 8 9.920542635658919 1110 9 10.19961240310078 1110 10-11 -0.8507751937984516 1110 12-13 -3.4563953488372086 1110 14-15 -2.844961240310081 1110 16-17 7.58914728682171 1110 18-19 3.3895348837209305 1110 20-21 -5.5339147286821735 1110 22-23 1.6027131782945716 1110 24-25 -5.357558139534884 1110 26-27 -0.53585271317829 1110 28-29 11.804263565891475 1110 30-31 3.1511627906976756 1110 32-33 6.097868217054266 1110 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52-53 -16.459302325581394 2313 54-55 -16.66763565891473 2313 56-57 -16.483527131782946 2313 58-59 -15.197674418604652 2313 60-61 -13.647286821705427 2313 62-63 -13.794573643410851 2313 64-65 -14.006782945736433 2313 66-67 -14.032945736434108 2313 68-69 -13.021317829457363 2313 70-71 -13.437015503875967 2313 72-73 -12.301356589147288 2313 74-75 -11.17732558139535 2313 76-77 -10.623062015503878 2313 78-79 -10.48546511627907 2313 80-81 -10.017441860465116 2313 82-83 -7.468023255813954 2313 84-85 -6.922480620155039 2313 86-87 -6.39922480620155 2313 88-89 -5.840116279069767 2313 90-91 -5.6443798449612395 2313 92-93 -5.291666666666667 2313 94-95 -4.531976744186046 2313 96-97 -3.770348837209302 2313 98-99 -3.950581395348837 2313 100-101 -2.6889534883720927 2314 1 -6.7984496124031 2314 2 10.251937984496124 2314 3 9.9515503875969 2314 4 -7.441860465116278 2314 5 6.608527131782942 2314 6 8.591085271317827 2314 7 -6.443798449612405 2314 8 7.920542635658919 2314 9 3.1996124031007795 2314 10-11 7.649224806201548 2314 12-13 7.043604651162791 2314 14-15 11.15503875968992 2314 16-17 10.08914728682171 2314 18-19 11.88953488372093 2314 20-21 12.966085271317827 2314 22-23 8.102713178294572 2314 24-25 4.142441860465116 2314 26-27 3.96414728682171 2314 28-29 -5.195736434108525 2314 30-31 -0.3488372093023244 2314 32-33 -8.402131782945734 2314 34-35 -4.684108527131784 2314 36-37 -2.3594961240310077 2314 38-39 1.941860465116278 2314 40-41 -5.367248062015502 2314 42-43 -5.306201550387595 2314 44-45 -0.33527131782945574 2314 46-47 13.900193798449614 2314 48-49 13.181201550387595 2314 50-51 10.343992248062015 2314 52-53 -16.459302325581394 2314 54-55 -16.66763565891473 2314 56-57 -16.483527131782946 2314 58-59 -15.197674418604652 2314 60-61 -13.647286821705427 2314 62-63 -13.794573643410851 2314 64-65 -14.006782945736433 2314 66-67 -14.032945736434108 2314 68-69 -13.021317829457363 2314 70-71 -13.437015503875967 2314 72-73 -12.301356589147288 2314 74-75 -11.17732558139535 2314 76-77 -10.623062015503878 2314 78-79 -10.48546511627907 2314 80-81 -10.017441860465116 2314 82-83 -7.468023255813954 2314 84-85 -6.922480620155039 2314 86-87 -6.39922480620155 2314 88-89 -5.840116279069767 2314 90-91 -5.6443798449612395 2314 92-93 -5.291666666666667 2314 94-95 -4.531976744186046 2314 96-97 -3.770348837209302 2314 98-99 -3.950581395348837 2314 100-101 -2.6889534883720927 2315 1 8.2015503875969 2315 2 7.251937984496124 2315 3 7.9515503875969 2315 4 10.558139534883722 2315 5 10.608527131782942 2315 6 8.591085271317827 2315 7 11.556201550387595 2315 8 9.920542635658919 2315 9 8.19961240310078 2315 10-11 11.649224806201548 2315 12-13 14.043604651162791 2315 14-15 14.15503875968992 2315 16-17 13.58914728682171 2315 18-19 15.38953488372093 2315 20-21 16.466085271317827 2315 22-23 17.10271317829457 2315 24-25 15.142441860465116 2315 26-27 15.46414728682171 2315 28-29 13.804263565891475 2315 30-31 12.151162790697676 2315 32-33 3.597868217054266 2315 34-35 10.815891472868216 2315 36-37 12.640503875968992 2315 38-39 12.441860465116278 2315 40-41 -5.367248062015502 2315 42-43 -18.306201550387595 2315 44-45 -18.335271317829456 2315 46-47 -18.099806201550386 2315 48-49 -17.818798449612405 2315 50-51 -16.656007751937985 2315 52-53 -16.459302325581394 2315 54-55 -16.66763565891473 2315 56-57 -16.483527131782946 2315 58-59 -15.197674418604652 2315 60-61 -13.647286821705427 2315 62-63 -13.794573643410851 2315 64-65 -14.006782945736433 2315 66-67 -14.032945736434108 2315 68-69 -13.021317829457363 2315 70-71 -13.437015503875967 2315 72-73 -12.301356589147288 2315 74-75 -11.17732558139535 2315 76-77 -10.623062015503878 2315 78-79 -10.48546511627907 2315 80-81 -10.017441860465116 2315 82-83 -7.468023255813954 2315 84-85 -6.922480620155039 2315 86-87 -6.39922480620155 2315 88-89 -5.840116279069767 2315 90-91 -5.6443798449612395 2315 92-93 -5.291666666666667 2315 94-95 -4.531976744186046 2315 96-97 -3.770348837209302 2315 98-99 -3.950581395348837 2315 100-101 -2.6889534883720927 2316 1 10.2015503875969 2316 2 2.2519379844961236 2316 3 9.9515503875969 2316 4 8.558139534883722 2316 5 8.608527131782942 2316 6 8.591085271317827 2316 7 7.556201550387595 2316 8 9.920542635658919 2316 9 12.19961240310078 2316 10-11 11.649224806201548 2316 12-13 14.043604651162791 2316 14-15 11.65503875968992 2316 16-17 7.08914728682171 2316 18-19 -11.11046511627907 2316 20-21 6.4660852713178265 2316 22-23 14.602713178294572 2316 24-25 12.142441860465116 2316 26-27 13.46414728682171 2316 28-29 11.304263565891475 2316 30-31 16.651162790697676 2316 32-33 12.097868217054266 2316 34-35 11.315891472868216 2316 36-37 12.640503875968992 2316 38-39 10.441860465116278 2316 40-41 12.132751937984498 2316 42-43 14.693798449612405 2316 44-45 -2.8352713178294557 2316 46-47 -18.099806201550386 2316 48-49 -17.818798449612405 2316 50-51 -16.656007751937985 2316 52-53 -16.459302325581394 2316 54-55 -16.66763565891473 2316 56-57 -16.483527131782946 2316 58-59 -15.197674418604652 2316 60-61 -13.647286821705427 2316 62-63 -13.794573643410851 2316 64-65 -14.006782945736433 2316 66-67 -14.032945736434108 2316 68-69 -13.021317829457363 2316 70-71 -13.437015503875967 2316 72-73 -12.301356589147288 2316 74-75 -11.17732558139535 2316 76-77 -10.623062015503878 2316 78-79 -10.48546511627907 2316 80-81 -10.017441860465116 2316 82-83 -7.468023255813954 2316 84-85 -6.922480620155039 2316 86-87 -6.39922480620155 2316 88-89 -5.840116279069767 2316 90-91 -5.6443798449612395 2316 92-93 -5.291666666666667 2316 94-95 -4.531976744186046 2316 96-97 -3.770348837209302 2316 98-99 -3.950581395348837 2316 100-101 -2.6889534883720927 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 190.0 3 43.0 4 37.0 5 16.0 6 22.0 7 17.0 8 16.0 9 19.0 10 24.0 11 19.0 12 22.0 13 22.0 14 19.0 15 31.0 16 26.0 17 17.0 18 28.0 19 22.0 20 24.0 21 29.0 22 22.0 23 15.0 24 26.0 25 22.0 26 21.0 27 30.0 28 38.0 29 32.0 30 23.0 31 25.0 32 27.0 33 25.0 34 24.0 35 30.0 36 13.0 37 12.0 38 6.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.05314009661836 20.28985507246377 14.879227053140095 17.77777777777778 2 18.067632850241548 22.22222222222222 38.84057971014493 20.869565217391305 3 23.864734299516908 26.859903381642514 27.632850241545892 21.642512077294686 4 14.106280193236714 19.613526570048307 33.52657004830918 32.7536231884058 5 15.36231884057971 35.65217391304348 32.65700483091788 16.32850241545894 6 31.11111111111111 34.106280193236714 18.84057971014493 15.942028985507244 7 27.342995169082123 36.328502415458935 19.710144927536234 16.618357487922705 8 27.82608695652174 34.20289855072463 18.357487922705314 19.613526570048307 9 28.792270531400966 18.84057971014493 18.937198067632853 33.429951690821255 10-11 25.65217391304348 27.72946859903382 24.58937198067633 22.028985507246375 12-13 28.212560386473427 26.03864734299517 26.618357487922705 19.130434782608695 14-15 26.956521739130434 26.28019323671498 28.26086956521739 18.5024154589372 16-17 21.73913043478261 29.565217391304348 28.550724637681157 20.144927536231886 18-19 20.483091787439616 28.357487922705317 31.4975845410628 19.66183574879227 20-21 24.057971014492754 23.96135265700483 34.2512077294686 17.729468599033815 22-23 22.270531400966185 23.816425120772948 35.70048309178744 18.21256038647343 24-25 21.690821256038646 25.02415458937198 34.009661835748794 19.275362318840582 26-27 23.333333333333332 24.2512077294686 35.02415458937198 17.391304347826086 28-29 22.51207729468599 24.92753623188406 34.05797101449276 18.5024154589372 30-31 21.690821256038646 25.120772946859905 33.091787439613526 20.096618357487923 32-33 20.77294685990338 24.58937198067633 35.02415458937198 19.613526570048307 34-35 21.014492753623188 24.830917874396135 35.02415458937198 19.130434782608695 36-37 21.497584541062803 24.396135265700483 34.734299516908216 19.3719806763285 38-39 20.724637681159418 23.285024154589372 36.231884057971016 19.758454106280194 40-41 19.275362318840582 24.782608695652176 37.391304347826086 18.55072463768116 42-43 20.531400966183575 25.31400966183575 34.2512077294686 19.903381642512077 44-45 19.951690821256037 24.2512077294686 35.748792270531396 20.048309178743963 46-47 21.594202898550723 25.41062801932367 33.091787439613526 19.903381642512077 48-49 21.73913043478261 26.42512077294686 30.966183574879224 20.869565217391305 50-51 20.67632850241546 26.859903381642514 31.207729468599034 21.256038647342994 52-53 20.6280193236715 26.231884057971016 30.289855072463766 22.85024154589372 54-55 21.690821256038646 24.154589371980677 31.30434782608696 22.85024154589372 56-57 22.51207729468599 24.20289855072464 31.40096618357488 21.884057971014492 58-59 23.333333333333332 23.71980676328502 30.966183574879224 21.980676328502415 60-61 22.560386473429954 25.507246376811594 30.04830917874396 21.884057971014492 62-63 22.22222222222222 22.51207729468599 31.44927536231884 23.816425120772948 64-65 21.256038647342994 22.367149758454104 31.545893719806763 24.830917874396135 66-67 21.304347826086957 24.782608695652176 31.30434782608696 22.608695652173914 68-69 21.207729468599034 24.541062801932366 29.565217391304348 24.685990338164252 70-71 20.434782608695652 23.71980676328502 29.42028985507246 26.42512077294686 72-73 20.579710144927535 24.2512077294686 31.594202898550726 23.574879227053138 74-75 21.642512077294686 23.91304347826087 30.579710144927535 23.864734299516908 76-77 21.11111111111111 23.768115942028984 30.096618357487927 25.02415458937198 78-79 21.15942028985507 23.333333333333332 29.082125603864732 26.42512077294686 80-81 22.560386473429954 23.091787439613526 31.642512077294686 22.705314009661837 82-83 22.657004830917874 23.18840579710145 28.695652173913043 25.458937198067634 84-85 23.816425120772948 25.845410628019323 26.763285024154587 23.574879227053138 86-87 22.608695652173914 25.845410628019323 25.60386473429952 25.94202898550725 88-89 21.642512077294686 23.71980676328502 28.454106280193237 26.183574879227052 90-91 24.92753623188406 22.367149758454104 25.217391304347824 27.48792270531401 92-93 26.811594202898554 21.594202898550723 24.396135265700483 27.198067632850243 94-95 26.28019323671498 21.44927536231884 26.521739130434785 25.7487922705314 96-97 27.149758454106284 20.241545893719806 26.183574879227052 26.42512077294686 98-99 25.507246376811594 20.917874396135268 26.521739130434785 27.053140096618357 100-101 25.31400966183575 22.753623188405797 27.246376811594203 24.685990338164252 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.5 6 0.5 7 0.5 8 2.5 9 2.0 10 1.0 11 2.5 12 3.0 13 4.0 14 4.5 15 3.5 16 5.0 17 6.0 18 4.5 19 4.5 20 5.5 21 7.0 22 7.0 23 5.5 24 4.0 25 6.0 26 8.5 27 8.5 28 9.5 29 11.0 30 10.0 31 8.5 32 11.0 33 12.0 34 13.5 35 15.5 36 17.0 37 15.0 38 18.5 39 25.0 40 24.5 41 24.0 42 23.0 43 27.5 44 32.5 45 34.0 46 37.5 47 43.0 48 44.5 49 43.5 50 45.5 51 51.0 52 51.0 53 38.5 54 34.5 55 37.0 56 30.5 57 23.5 58 19.0 59 18.5 60 16.0 61 11.5 62 10.0 63 7.5 64 6.0 65 5.0 66 5.0 67 6.0 68 4.0 69 2.5 70 2.5 71 3.0 72 2.5 73 1.5 74 1.5 75 1.0 76 0.5 77 0.5 78 0.5 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1035.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.71497584541063 #Duplication Level Percentage of deduplicated Percentage of total 1 99.000999000999 95.7487922705314 2 0.4995004995004995 0.966183574879227 3 0.0999000999000999 0.2898550724637681 4 0.0999000999000999 0.3864734299516908 5 0.0 0.0 6 0.0 0.0 7 0.0999000999000999 0.6763285024154589 8 0.0 0.0 9 0.0999000999000999 0.8695652173913043 >10 0.0999000999000999 1.0628019323671498 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 1.0628019323671498 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.8695652173913043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.6763285024154589 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.3864734299516908 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.2898550724637681 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1932367149758454 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2 0.1932367149758454 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT 2 0.1932367149758454 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1932367149758454 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1932367149758454 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0966183574879227 0.0 26-27 0.0 0.0 0.0 0.24154589371980675 0.0 28-29 0.0 0.0 0.0 0.8212560386473431 0.0 30-31 0.0 0.0 0.0 1.9806763285024154 0.0 32-33 0.0 0.0 0.0 3.236714975845411 0.0 34-35 0.0 0.0 0.0 4.29951690821256 0.0 36-37 0.0 0.0 0.0 5.748792270531401 0.0 38-39 0.0 0.0 0.0 7.4396135265700485 0.0 40-41 0.0 0.0 0.0 8.93719806763285 0.0 42-43 0.0 0.0 0.0 10.193236714975846 0.0 44-45 0.0 0.0 0.0 10.72463768115942 0.0 46-47 0.0 0.0 0.0 11.207729468599034 0.0 48-49 0.0 0.0 0.0 11.884057971014492 0.0 50-51 0.0 0.0 0.0 12.946859903381643 0.0 52-53 0.0 0.0 0.0 14.63768115942029 0.0 54-55 0.0 0.0 0.0 15.217391304347826 0.0 56-57 0.0 0.0 0.0 17.77777777777778 0.0 58-59 0.0 0.0 0.0 19.130434782608695 0.0 60-61 0.0 0.0 0.0 20.096618357487923 0.0 62-63 0.0 0.0 0.0 20.82125603864734 0.0 64-65 0.0 0.0 0.0 22.85024154589372 0.0 66-67 0.0 0.0 0.0 24.734299516908212 0.0 68-69 0.0 0.0 0.0 25.265700483091788 0.0 70-71 0.0 0.0 0.0 27.294685990338166 0.0 72-73 0.0 0.0 0.0 30.0 0.0 74-75 0.0 0.0 0.0 31.40096618357488 0.0 76-77 0.0 0.0 0.0 32.367149758454104 0.0 78-79 0.0 0.0 0.0 32.850241545893724 0.0 80-81 0.0 0.0 0.0 33.47826086956522 0.0 82-83 0.0 0.0 0.0 33.71980676328502 0.0 84-85 0.0 0.0 0.0 34.00966183574879 0.0 86-87 0.0 0.0 0.0 34.106280193236714 0.0 88-89 0.0 0.0 0.0 34.20289855072464 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE