SRR937294_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR937294_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1011
Sequences flagged as poor quality	0
Sequence length	101
%GC	42

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	13	1.2858555885262115	No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT	12	1.1869436201780417	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	11	1.0880316518298714	No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT	10	0.9891196834817012	No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT	10	0.9891196834817012	RNA PCR Primer, Index 31 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	9	0.8902077151335311	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	8	0.791295746785361	TruSeq Adapter, Index 3 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.791295746785361	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.791295746785361	No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG	8	0.791295746785361	RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT	7	0.6923837784371909	RNA PCR Primer, Index 3 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	6	0.5934718100890208	No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT	6	0.5934718100890208	RNA PCR Primer, Index 31 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT	6	0.5934718100890208	RNA PCR Primer, Index 23 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.5934718100890208	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	6	0.5934718100890208	RNA PCR Primer, Index 33 (95% over 22bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG	5	0.4945598417408506	TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	5	0.4945598417408506	No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	5	0.4945598417408506	RNA PCR Primer, Index 26 (100% over 22bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG	4	0.3956478733926805	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	4	0.3956478733926805	No Hit
CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA	4	0.3956478733926805	No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT	4	0.3956478733926805	RNA PCR Primer, Index 33 (95% over 24bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT	4	0.3956478733926805	RNA PCR Primer, Index 31 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	4	0.3956478733926805	RNA PCR Primer, Index 26 (95% over 22bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG	4	0.3956478733926805	RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG	4	0.3956478733926805	TruSeq Adapter, Index 1 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT	4	0.3956478733926805	RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG	4	0.3956478733926805	RNA PCR Primer, Index 33 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT	3	0.2967359050445104	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG	3	0.2967359050445104	RNA PCR Primer, Index 15 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC	3	0.2967359050445104	No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT	3	0.2967359050445104	RNA PCR Primer, Index 23 (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	3	0.2967359050445104	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.2967359050445104	No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	3	0.2967359050445104	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	3	0.2967359050445104	RNA PCR Primer, Index 27 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	3	0.2967359050445104	No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT	3	0.2967359050445104	RNA PCR Primer, Index 23 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT	3	0.2967359050445104	No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG	3	0.2967359050445104	RNA PCR Primer, Index 38 (95% over 24bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG	3	0.2967359050445104	RNA PCR Primer, Index 27 (96% over 30bp)
GCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACT	3	0.2967359050445104	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	2	0.19782393669634024	No Hit
GTCCATGACCATTAACTGGAGCCTTTTCAGCCCTCCTTCTAACATCAGGT	2	0.19782393669634024	No Hit
TCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAA	2	0.19782393669634024	Illumina PCR Primer Index 1 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT	2	0.19782393669634024	RNA PCR Primer, Index 46 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	2	0.19782393669634024	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	2	0.19782393669634024	TruSeq Adapter, Index 11 (95% over 21bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT	2	0.19782393669634024	RNA PCR Primer, Index 23 (96% over 28bp)
ACATGTAACAAGTTAAGTCCTAGCATCTAGTGATTTGGGTTAGGTTACAC	2	0.19782393669634024	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA	2	0.19782393669634024	RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT	2	0.19782393669634024	RNA PCR Primer, Index 1 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT	2	0.19782393669634024	RNA PCR Primer, Index 26 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC	2	0.19782393669634024	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	2	0.19782393669634024	RNA PCR Primer, Index 39 (95% over 23bp)
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC	2	0.19782393669634024	Illumina PCR Primer Index 11 (96% over 26bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG	2	0.19782393669634024	TruSeq Adapter, Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGGCTT	2	0.19782393669634024	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT	2	0.19782393669634024	No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC	2	0.19782393669634024	No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT	2	0.19782393669634024	RNA PCR Primer, Index 31 (95% over 21bp)
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG	2	0.19782393669634024	RNA PCR Primer, Index 27 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	2	0.19782393669634024	No Hit
CTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATG	2	0.19782393669634024	No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT	2	0.19782393669634024	No Hit

Adapter Content

Adapter graph

Kmer Content

No overrepresented Kmers

Summary