##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937293_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1157 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.694900605012965 30.0 16.0 31.0 2.0 33.0 2 23.763180639585133 30.0 16.0 31.0 2.0 33.0 3 23.432152117545375 30.0 16.0 31.0 2.0 33.0 4 26.061365600691442 33.0 19.0 35.0 2.0 37.0 5 26.02247191011236 33.0 19.0 35.0 2.0 37.0 6 25.79515989628349 33.0 17.0 35.0 2.0 37.0 7 25.593777009507345 33.0 17.0 35.0 2.0 37.0 8 25.485738980121003 33.0 15.0 35.0 2.0 37.0 9 26.225583405358687 33.0 15.0 37.0 2.0 39.0 10-11 25.98401037165082 32.5 11.0 37.0 2.0 39.0 12-13 25.42437337942956 32.0 10.0 37.0 2.0 39.0 14-15 25.373379429559204 32.0 10.0 38.0 2.0 40.0 16-17 25.296456352636127 32.0 10.0 38.0 2.0 40.0 18-19 25.37381158167675 32.0 9.5 38.0 2.0 40.0 20-21 25.510803802938632 32.5 8.0 38.0 2.0 40.0 22-23 25.04494382022472 32.5 2.0 38.0 2.0 40.0 24-25 24.89628349178911 32.0 2.0 38.0 2.0 40.0 26-27 24.534140017286084 31.5 2.0 38.0 2.0 40.0 28-29 24.375972342264475 31.0 2.0 38.0 2.0 40.0 30-31 23.6637856525497 31.0 2.0 37.0 2.0 39.0 32-33 23.218236819360413 30.5 2.0 36.5 2.0 39.0 34-35 22.70829732065687 30.0 2.0 35.5 2.0 39.0 36-37 22.4019014693172 30.0 2.0 36.0 2.0 39.0 38-39 22.013828867761454 30.0 2.0 35.0 2.0 39.0 40-41 21.675885911840965 29.0 2.0 35.0 2.0 39.0 42-43 21.146067415730336 28.0 2.0 35.0 2.0 39.0 44-45 20.644338807260155 26.5 2.0 35.5 2.0 39.0 46-47 20.100691443388072 25.0 2.0 35.0 2.0 38.5 48-49 19.522904062229905 23.0 2.0 35.0 2.0 39.0 50-51 18.67286084701815 22.5 2.0 34.0 2.0 38.0 52-53 18.799481417458946 22.0 2.0 35.0 2.0 38.5 54-55 18.8876404494382 21.5 2.0 35.0 2.0 39.0 56-57 18.2333621434745 16.0 2.0 35.0 2.0 39.0 58-59 17.870354364736386 10.5 2.0 35.0 2.0 39.0 60-61 17.493949870354363 7.5 2.0 34.5 2.0 39.0 62-63 16.953759723422642 2.0 2.0 34.0 2.0 38.0 64-65 16.237683664649957 2.0 2.0 34.0 2.0 38.0 66-67 16.080380293863442 2.0 2.0 33.5 2.0 37.5 68-69 15.337510803802939 2.0 2.0 32.5 2.0 36.5 70-71 14.585566119273984 2.0 2.0 32.0 2.0 36.0 72-73 14.05877268798617 2.0 2.0 31.0 2.0 36.0 74-75 13.700518582541054 2.0 2.0 31.0 2.0 35.5 76-77 13.233362143474503 2.0 2.0 31.0 2.0 35.0 78-79 12.737683664649957 2.0 2.0 30.0 2.0 35.0 80-81 12.419619706136562 2.0 2.0 29.5 2.0 35.0 82-83 11.662057044079516 2.0 2.0 28.5 2.0 34.5 84-85 11.111495246326708 2.0 2.0 26.0 2.0 34.0 86-87 10.363439930855662 2.0 2.0 25.0 2.0 34.0 88-89 9.7990492653414 2.0 2.0 21.5 2.0 33.5 90-91 9.227312013828868 2.0 2.0 14.5 2.0 33.0 92-93 8.757130509939499 2.0 2.0 4.5 2.0 33.0 94-95 8.16810717372515 2.0 2.0 2.0 2.0 31.0 96-97 7.747191011235955 2.0 2.0 2.0 2.0 31.0 98-99 7.043647363872083 2.0 2.0 2.0 2.0 30.5 100-101 6.502160760587727 2.0 2.0 2.0 2.0 29.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -21.13186813186813 1101 2 -20.50732600732601 1101 3 -20.576923076923077 1101 4 -21.304029304029307 1101 5 -21.84798534798535 1101 6 -22.093406593406595 1101 7 -22.051282051282055 1101 8 -22.919413919413923 1101 9 -23.877289377289372 1101 10-11 -23.695054945054945 1101 12-13 -22.872710622710628 1101 14-15 -23.294871794871792 1101 16-17 -22.436813186813186 1101 18-19 -22.604395604395606 1101 20-21 -23.912087912087912 1101 22-23 -22.744505494505493 1101 24-25 -22.73901098901099 1101 26-27 -22.066849816849814 1101 28-29 -21.787545787545792 1101 30-31 -21.551282051282055 1101 32-33 -21.061355311355307 1101 34-35 -20.934981684981683 1101 36-37 -21.26190476190476 1101 38-39 -20.48076923076923 1101 40-41 -19.94047619047619 1101 42-43 -19.0503663003663 1101 44-45 -20.104395604395606 1101 46-47 -19.27014652014652 1101 48-49 -19.074175824175825 1101 50-51 -18.493589743589745 1101 52-53 -18.05311355311355 1101 54-55 -18.653846153846153 1101 56-57 -17.608058608058606 1101 58-59 -17.343406593406595 1101 60-61 -17.03846153846154 1101 62-63 -17.142857142857142 1101 64-65 -16.84157509157509 1101 66-67 -16.652930402930405 1101 68-69 -15.850732600732602 1101 70-71 -15.147435897435898 1101 72-73 -14.860805860805861 1101 74-75 -14.157509157509157 1101 76-77 -14.40934065934066 1101 78-79 -13.602564102564102 1101 80-81 -12.717032967032967 1101 82-83 -10.81043956043956 1101 84-85 -10.563186813186814 1101 86-87 -10.438644688644686 1101 88-89 -9.739010989010989 1101 90-91 -8.53937728937729 1101 92-93 -7.1007326007326 1101 94-95 -6.242673992673993 1101 96-97 -5.633699633699633 1101 98-99 -5.345238095238096 1101 100-101 -4.649267399267399 1103 1 4.868131868131869 1103 2 -2.007326007326011 1103 3 0.9230769230769234 1103 4 -0.8040293040293065 1103 5 7.65201465201465 1103 6 8.406593406593405 1103 7 11.948717948717945 1103 8 -1.4194139194139233 1103 9 7.122710622710628 1103 10-11 8.054945054945055 1103 12-13 -0.3727106227106276 1103 14-15 -1.044871794871792 1103 16-17 4.563186813186814 1103 18-19 3.145604395604394 1103 20-21 0.8379120879120876 1103 22-23 7.005494505494507 1103 24-25 6.010989010989011 1103 26-27 2.683150183150186 1103 28-29 10.712454212454208 1103 30-31 11.948717948717945 1103 32-33 12.938644688644693 1103 34-35 -4.4349816849816825 1103 36-37 -5.261904761904759 1103 38-39 -13.23076923076923 1103 40-41 -19.94047619047619 1103 42-43 -19.0503663003663 1103 44-45 -20.104395604395606 1103 46-47 -19.27014652014652 1103 48-49 -19.074175824175825 1103 50-51 -18.493589743589745 1103 52-53 -18.05311355311355 1103 54-55 -18.653846153846153 1103 56-57 -17.608058608058606 1103 58-59 -17.343406593406595 1103 60-61 -17.03846153846154 1103 62-63 -17.142857142857142 1103 64-65 -16.84157509157509 1103 66-67 -16.652930402930405 1103 68-69 -15.850732600732602 1103 70-71 -15.147435897435898 1103 72-73 -14.860805860805861 1103 74-75 -14.157509157509157 1103 76-77 -14.40934065934066 1103 78-79 -13.602564102564102 1103 80-81 -12.717032967032967 1103 82-83 -10.81043956043956 1103 84-85 -10.563186813186814 1103 86-87 -10.438644688644686 1103 88-89 -9.739010989010989 1103 90-91 -8.53937728937729 1103 92-93 -7.1007326007326 1103 94-95 -6.242673992673993 1103 96-97 -5.633699633699633 1103 98-99 -5.345238095238096 1103 100-101 -4.649267399267399 1104 1 -7.131868131868131 1104 2 10.49267399267399 1104 3 2.4230769230769234 1104 4 9.695970695970693 1104 5 11.15201465201465 1104 6 12.906593406593405 1104 7 -14.051282051282055 1104 8 8.080586080586077 1104 9 8.122710622710628 1104 10-11 12.304945054945055 1104 12-13 7.127289377289372 1104 14-15 8.705128205128208 1104 16-17 5.063186813186814 1104 18-19 2.395604395604394 1104 20-21 3.5879120879120876 1104 22-23 8.755494505494507 1104 24-25 12.260989010989011 1104 26-27 10.933150183150186 1104 28-29 10.712454212454208 1104 30-31 9.948717948717945 1104 32-33 -11.061355311355307 1104 34-35 7.0650183150183175 1104 36-37 7.238095238095241 1104 38-39 9.51923076923077 1104 40-41 6.05952380952381 1104 42-43 3.4496336996337007 1104 44-45 13.895604395604394 1104 46-47 5.729853479853482 1104 48-49 15.925824175824175 1104 50-51 18.006410256410255 1104 52-53 13.446886446886449 1104 54-55 15.346153846153847 1104 56-57 12.891941391941394 1104 58-59 8.656593406593405 1104 60-61 14.96153846153846 1104 62-63 15.857142857142858 1104 64-65 20.15842490842491 1104 66-67 11.847069597069595 1104 68-69 -0.8507326007326022 1104 70-71 -15.147435897435898 1104 72-73 -14.860805860805861 1104 74-75 -14.157509157509157 1104 76-77 -14.40934065934066 1104 78-79 -13.602564102564102 1104 80-81 -12.717032967032967 1104 82-83 -10.81043956043956 1104 84-85 -10.563186813186814 1104 86-87 -10.438644688644686 1104 88-89 -9.739010989010989 1104 90-91 -8.53937728937729 1104 92-93 -7.1007326007326 1104 94-95 -6.242673992673993 1104 96-97 -5.633699633699633 1104 98-99 -5.345238095238096 1104 100-101 -4.649267399267399 1105 1 6.868131868131869 1105 2 8.49267399267399 1105 3 8.423076923076923 1105 4 -4.3040293040293065 1105 5 8.15201465201465 1105 6 10.906593406593405 1105 7 10.948717948717945 1105 8 12.080586080586077 1105 9 9.122710622710628 1105 10-11 6.304945054945055 1105 12-13 14.127289377289372 1105 14-15 10.705128205128208 1105 16-17 9.063186813186814 1105 18-19 14.395604395604394 1105 20-21 8.587912087912088 1105 22-23 13.255494505494507 1105 24-25 0.26098901098901095 1105 26-27 1.933150183150186 1105 28-29 7.712454212454208 1105 30-31 -4.551282051282055 1105 32-33 6.938644688644693 1105 34-35 8.565018315018317 1105 36-37 13.23809523809524 1105 38-39 12.01923076923077 1105 40-41 12.05952380952381 1105 42-43 10.9496336996337 1105 44-45 5.895604395604394 1105 46-47 12.729853479853482 1105 48-49 14.425824175824175 1105 50-51 14.506410256410255 1105 52-53 15.946886446886449 1105 54-55 10.846153846153847 1105 56-57 11.891941391941394 1105 58-59 15.156593406593405 1105 60-61 14.46153846153846 1105 62-63 10.857142857142858 1105 64-65 15.158424908424909 1105 66-67 14.347069597069595 1105 68-69 13.649267399267398 1105 70-71 13.352564102564102 1105 72-73 9.139194139194139 1105 74-75 18.342490842490843 1105 76-77 18.59065934065934 1105 78-79 16.397435897435898 1105 80-81 15.282967032967033 1105 82-83 17.689560439560438 1105 84-85 -10.563186813186814 1105 86-87 -10.438644688644686 1105 88-89 -9.739010989010989 1105 90-91 -8.53937728937729 1105 92-93 -7.1007326007326 1105 94-95 -6.242673992673993 1105 96-97 -5.633699633699633 1105 98-99 -5.345238095238096 1105 100-101 -4.649267399267399 1107 1 -3.1318681318681314 1107 2 5.492673992673989 1107 3 7.423076923076923 1107 4 3.6959706959706935 1107 5 7.65201465201465 1107 6 7.906593406593405 1107 7 -6.551282051282055 1107 8 3.0805860805860767 1107 9 5.622710622710628 1107 10-11 -5.695054945054945 1107 12-13 5.377289377289372 1107 14-15 0.705128205128208 1107 16-17 2.313186813186814 1107 18-19 0.1456043956043942 1107 20-21 0.8379120879120876 1107 22-23 -4.494505494505493 1107 24-25 -0.989010989010989 1107 26-27 7.933150183150186 1107 28-29 -5.287545787545792 1107 30-31 -14.051282051282055 1107 32-33 -11.811355311355307 1107 34-35 -6.4349816849816825 1107 36-37 -7.761904761904759 1107 38-39 -2.73076923076923 1107 40-41 -6.19047619047619 1107 42-43 -2.5503663003662993 1107 44-45 -10.104395604395606 1107 46-47 -4.770146520146518 1107 48-49 -1.8241758241758248 1107 50-51 -6.4935897435897445 1107 52-53 -4.053113553113551 1107 54-55 -4.903846153846153 1107 56-57 -0.8580586080586059 1107 58-59 -4.343406593406595 1107 60-61 -2.53846153846154 1107 62-63 -4.892857142857142 1107 64-65 -1.5915750915750912 1107 66-67 -9.402930402930405 1107 68-69 -15.850732600732602 1107 70-71 -15.147435897435898 1107 72-73 -14.860805860805861 1107 74-75 -14.157509157509157 1107 76-77 -14.40934065934066 1107 78-79 -13.602564102564102 1107 80-81 -12.717032967032967 1107 82-83 -10.81043956043956 1107 84-85 -10.563186813186814 1107 86-87 -10.438644688644686 1107 88-89 -9.739010989010989 1107 90-91 -8.53937728937729 1107 92-93 -7.1007326007326 1107 94-95 -6.242673992673993 1107 96-97 -5.633699633699633 1107 98-99 -5.345238095238096 1107 100-101 -4.649267399267399 1108 1 10.868131868131869 1108 2 11.49267399267399 1108 3 11.423076923076923 1108 4 13.695970695970693 1108 5 13.15201465201465 1108 6 12.906593406593405 1108 7 12.948717948717945 1108 8 12.080586080586077 1108 9 13.122710622710628 1108 10-11 13.304945054945055 1108 12-13 13.127289377289372 1108 14-15 15.705128205128208 1108 16-17 16.063186813186814 1108 18-19 16.395604395604394 1108 20-21 14.087912087912088 1108 22-23 16.255494505494507 1108 24-25 16.26098901098901 1108 26-27 16.933150183150186 1108 28-29 17.212454212454208 1108 30-31 17.448717948717945 1108 32-33 17.938644688644693 1108 34-35 18.065018315018317 1108 36-37 17.23809523809524 1108 38-39 18.01923076923077 1108 40-41 18.55952380952381 1108 42-43 19.9496336996337 1108 44-45 18.895604395604394 1108 46-47 19.72985347985348 1108 48-49 19.925824175824175 1108 50-51 20.006410256410255 1108 52-53 19.94688644688645 1108 54-55 20.346153846153847 1108 56-57 21.391941391941394 1108 58-59 21.656593406593405 1108 60-61 20.96153846153846 1108 62-63 21.357142857142858 1108 64-65 21.65842490842491 1108 66-67 22.347069597069595 1108 68-69 23.149267399267398 1108 70-71 23.852564102564102 1108 72-73 24.13919413919414 1108 74-75 23.842490842490843 1108 76-77 22.59065934065934 1108 78-79 23.397435897435898 1108 80-81 22.782967032967033 1108 82-83 24.189560439560438 1108 84-85 24.436813186813186 1108 86-87 22.561355311355314 1108 88-89 23.26098901098901 1108 90-91 24.460622710622708 1108 92-93 25.8992673992674 1108 94-95 26.757326007326007 1108 96-97 27.366300366300365 1108 98-99 25.654761904761905 1108 100-101 26.350732600732602 1109 1 9.868131868131869 1109 2 -12.50732600732601 1109 3 2.4230769230769234 1109 4 1.6959706959706935 1109 5 4.15201465201465 1109 6 8.906593406593405 1109 7 8.948717948717945 1109 8 10.080586080586077 1109 9 9.122710622710628 1109 10-11 9.304945054945055 1109 12-13 8.627289377289372 1109 14-15 9.205128205128208 1109 16-17 8.563186813186814 1109 18-19 4.395604395604394 1109 20-21 1.5879120879120876 1109 22-23 -3.7445054945054927 1109 24-25 -2.239010989010989 1109 26-27 1.433150183150186 1109 28-29 0.7124542124542081 1109 30-31 -7.051282051282055 1109 32-33 6.438644688644693 1109 34-35 10.065018315018317 1109 36-37 5.738095238095241 1109 38-39 7.51923076923077 1109 40-41 -2.44047619047619 1109 42-43 -9.0503663003663 1109 44-45 9.395604395604394 1109 46-47 -5.270146520146518 1109 48-49 6.925824175824175 1109 50-51 3.5064102564102555 1109 52-53 11.446886446886449 1109 54-55 3.8461538461538467 1109 56-57 14.391941391941394 1109 58-59 -3.843406593406595 1109 60-61 -5.53846153846154 1109 62-63 -1.1428571428571423 1109 64-65 -8.341575091575091 1109 66-67 -0.652930402930405 1109 68-69 8.649267399267398 1109 70-71 9.352564102564102 1109 72-73 19.63919413919414 1109 74-75 17.842490842490843 1109 76-77 8.09065934065934 1109 78-79 10.397435897435898 1109 80-81 15.782967032967033 1109 82-83 3.1895604395604398 1109 84-85 -10.563186813186814 1109 86-87 -10.438644688644686 1109 88-89 -9.739010989010989 1109 90-91 -8.53937728937729 1109 92-93 -7.1007326007326 1109 94-95 -6.242673992673993 1109 96-97 -5.633699633699633 1109 98-99 -5.345238095238096 1109 100-101 -4.649267399267399 1110 1 0.3681318681318686 1110 2 -1.0073260073260109 1110 3 -0.5769230769230766 1110 4 0.1959706959706935 1110 5 -0.3479853479853503 1110 6 -0.5934065934065949 1110 7 8.448717948717945 1110 8 8.580586080586077 1110 9 -4.377289377289372 1110 10-11 7.054945054945055 1110 12-13 2.1272893772893724 1110 14-15 -10.544871794871792 1110 16-17 -6.936813186813186 1110 18-19 -4.604395604395606 1110 20-21 -4.662087912087912 1110 22-23 -3.9945054945054927 1110 24-25 -5.239010989010989 1110 26-27 -3.066849816849814 1110 28-29 -4.787545787545792 1110 30-31 -5.801282051282055 1110 32-33 -2.8113553113553067 1110 34-35 -5.4349816849816825 1110 36-37 -3.261904761904759 1110 38-39 -1.73076923076923 1110 40-41 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10.857142857142858 2313 64-65 14.158424908424909 2313 66-67 11.847069597069595 2313 68-69 9.649267399267398 2313 70-71 3.352564102564102 2313 72-73 9.639194139194139 2313 74-75 14.842490842490843 2313 76-77 13.59065934065934 2313 78-79 9.397435897435898 2313 80-81 5.282967032967033 2313 82-83 5.18956043956044 2313 84-85 10.436813186813186 2313 86-87 18.061355311355314 2313 88-89 22.76098901098901 2313 90-91 6.46062271062271 2313 92-93 15.8992673992674 2313 94-95 19.257326007326007 2313 96-97 -5.633699633699633 2313 98-99 -5.345238095238096 2313 100-101 -4.649267399267399 2314 1 7.868131868131869 2314 2 8.49267399267399 2314 3 3.4230769230769234 2314 4 11.695970695970693 2314 5 8.15201465201465 2314 6 10.906593406593405 2314 7 7.948717948717945 2314 8 10.080586080586077 2314 9 11.122710622710628 2314 10-11 12.304945054945055 2314 12-13 13.127289377289372 2314 14-15 10.705128205128208 2314 16-17 13.563186813186814 2314 18-19 14.895604395604394 2314 20-21 11.587912087912088 2314 22-23 11.255494505494507 2314 24-25 13.260989010989011 2314 26-27 14.433150183150186 2314 28-29 8.212454212454208 2314 30-31 10.948717948717945 2314 32-33 10.938644688644693 2314 34-35 16.565018315018317 2314 36-37 13.73809523809524 2314 38-39 7.51923076923077 2314 40-41 16.05952380952381 2314 42-43 14.9496336996337 2314 44-45 17.395604395604394 2314 46-47 18.72985347985348 2314 48-49 16.925824175824175 2314 50-51 15.506410256410255 2314 52-53 19.94688644688645 2314 54-55 15.346153846153847 2314 56-57 17.891941391941394 2314 58-59 19.656593406593405 2314 60-61 19.46153846153846 2314 62-63 19.357142857142858 2314 64-65 16.65842490842491 2314 66-67 16.347069597069595 2314 68-69 21.149267399267398 2314 70-71 12.352564102564102 2314 72-73 8.139194139194139 2314 74-75 -1.157509157509157 2314 76-77 11.09065934065934 2314 78-79 2.397435897435898 2314 80-81 -12.717032967032967 2314 82-83 -10.81043956043956 2314 84-85 -10.563186813186814 2314 86-87 -10.438644688644686 2314 88-89 -9.739010989010989 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2315 50-51 6.0064102564102555 2315 52-53 8.446886446886449 2315 54-55 11.346153846153847 2315 56-57 12.391941391941394 2315 58-59 11.156593406593405 2315 60-61 9.46153846153846 2315 62-63 13.357142857142858 2315 64-65 16.15842490842491 2315 66-67 16.347069597069595 2315 68-69 16.149267399267398 2315 70-71 12.852564102564102 2315 72-73 11.139194139194139 2315 74-75 -3.157509157509157 2315 76-77 14.59065934065934 2315 78-79 6.897435897435898 2315 80-81 5.282967032967033 2315 82-83 16.689560439560438 2315 84-85 15.436813186813186 2315 86-87 19.561355311355314 2315 88-89 16.26098901098901 2315 90-91 18.960622710622708 2315 92-93 23.8992673992674 2315 94-95 -6.242673992673993 2315 96-97 -5.633699633699633 2315 98-99 -5.345238095238096 2315 100-101 -4.649267399267399 2316 1 7.868131868131869 2316 2 9.99267399267399 2316 3 9.923076923076923 2316 4 13.695970695970693 2316 5 11.15201465201465 2316 6 11.906593406593405 2316 7 12.948717948717945 2316 8 12.080586080586077 2316 9 7.122710622710628 2316 10-11 11.804945054945055 2316 12-13 13.627289377289372 2316 14-15 11.205128205128208 2316 16-17 11.813186813186814 2316 18-19 12.395604395604394 2316 20-21 13.837912087912088 2316 22-23 14.755494505494507 2316 24-25 14.510989010989011 2316 26-27 16.183150183150186 2316 28-29 15.962454212454208 2316 30-31 14.948717948717945 2316 32-33 15.438644688644693 2316 34-35 13.815018315018317 2316 36-37 14.73809523809524 2316 38-39 16.01923076923077 2316 40-41 16.30952380952381 2316 42-43 15.1996336996337 2316 44-45 16.895604395604394 2316 46-47 17.97985347985348 2316 48-49 18.925824175824175 2316 50-51 16.256410256410255 2316 52-53 17.44688644688645 2316 54-55 17.596153846153847 2316 56-57 19.641941391941394 2316 58-59 15.906593406593405 2316 60-61 19.71153846153846 2316 62-63 20.107142857142858 2316 64-65 18.40842490842491 2316 66-67 19.347069597069595 2316 68-69 20.649267399267398 2316 70-71 22.102564102564102 2316 72-73 20.88919413919414 2316 74-75 22.842490842490843 2316 76-77 20.09065934065934 2316 78-79 22.397435897435898 2316 80-81 21.282967032967033 2316 82-83 23.689560439560438 2316 84-85 23.436813186813186 2316 86-87 23.061355311355314 2316 88-89 23.76098901098901 2316 90-91 24.460622710622708 2316 92-93 23.3992673992674 2316 94-95 24.007326007326007 2316 96-97 21.366300366300365 2316 98-99 19.654761904761905 2316 100-101 20.350732600732602 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 192.0 3 37.0 4 33.0 5 21.0 6 17.0 7 15.0 8 22.0 9 17.0 10 14.0 11 23.0 12 19.0 13 31.0 14 31.0 15 38.0 16 33.0 17 34.0 18 25.0 19 17.0 20 20.0 21 24.0 22 26.0 23 24.0 24 38.0 25 21.0 26 28.0 27 35.0 28 32.0 29 35.0 30 30.0 31 33.0 32 39.0 33 28.0 34 32.0 35 35.0 36 25.0 37 23.0 38 9.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.360485268630846 19.064124783362217 18.370883882149048 16.204506065857885 2 16.724436741767764 22.270363951473136 40.81455805892548 20.19064124783362 3 21.23050259965338 29.20277296360485 27.902946273830153 21.663778162911612 4 14.917606244579359 18.99392888117953 34.431916738941894 31.656548135299218 5 14.038128249566725 35.181975736568454 33.015597920277294 17.76429809358752 6 27.989601386481805 39.68804159445407 16.464471403812826 15.857885615251298 7 27.209705372616984 34.1421143847487 20.45060658578856 18.19757365684575 8 26.8630849220104 32.06239168110919 18.717504332755635 22.357019064124785 9 30.589254766031193 16.81109185441941 18.80415944540728 33.795493934142115 10-11 24.48006932409012 26.429809358752166 25.433275563258235 23.656845753899482 12-13 29.636048526863085 27.4263431542461 24.74003466204506 18.19757365684575 14-15 25.17331022530329 26.03986135181976 27.902946273830153 20.88388214904679 16-17 21.403812824956674 29.246100519930675 27.642980935875215 21.707105719237436 18-19 21.057192374350088 25.563258232235704 35.48526863084922 17.89428076256499 20-21 22.790294627383016 23.700173310225303 37.045060658578855 16.464471403812826 22-23 21.100519930675908 24.74003466204506 34.40207972270364 19.75736568457539 24-25 22.660311958405547 25.34662045060659 34.40207972270364 17.590987868284227 26-27 20.19064124783362 26.213171577123052 35.09532062391681 18.500866551126517 28-29 21.36048526863085 25.17331022530329 34.22876949740035 19.23743500866551 30-31 20.363951473136915 24.653379549393414 37.21837088388215 17.76429809358752 32-33 21.49046793760832 23.743500866551127 37.56499133448873 17.20103986135182 34-35 22.207792207792206 26.75324675324675 33.939393939393945 17.0995670995671 36-37 20.45060658578856 24.696707105719238 35.13864818024263 19.714038128249566 38-39 20.40727902946274 25.30329289428076 35.65857885615251 18.630849220103986 40-41 19.62738301559792 25.563258232235704 34.79202772963605 20.01733102253033 42-43 19.49740034662045 25.99653379549394 35.35528596187175 19.150779896013866 44-45 20.103986135181977 24.176776429809358 34.575389948006936 21.143847487001732 46-47 20.797227036395146 24.436741767764296 34.22876949740035 20.537261698440208 48-49 20.103986135181977 25.0 34.27209705372617 20.623916811091856 50-51 19.05586834127328 26.93806842789086 31.139021221307928 22.867042009527935 52-53 20.095279341706366 26.67821567778259 31.702035513209182 21.524469467301863 54-55 21.79376083188908 25.34662045060659 32.668977469670715 20.19064124783362 56-57 21.447140381282495 24.393414211438476 30.979202772963603 23.180242634315423 58-59 22.487001733102254 22.573656845753902 31.412478336221838 23.526863084922013 60-61 22.530329289428074 24.436741767764296 32.06239168110919 20.97053726169844 62-63 21.013864818024263 21.88041594454073 33.23223570190642 23.873483535528596 64-65 19.844020797227035 22.920277296360485 32.712305025996535 24.523396880415945 66-67 21.49046793760832 24.653379549393414 32.712305025996535 21.143847487001732 68-69 21.187175043327557 23.786828422876948 32.10571923743501 22.920277296360485 70-71 18.80415944540728 25.476603119584055 32.06239168110919 23.656845753899482 72-73 19.670710571923745 24.263431542461007 32.62564991334489 23.440207972270365 74-75 19.62738301559792 25.0 32.32235701906412 23.050259965337954 76-77 19.49740034662045 24.090121317157713 30.459272097053724 25.953206239168107 78-79 21.013864818024263 22.0103986135182 31.06585788561525 25.909878682842287 80-81 20.233968804159446 22.400346620450605 33.36221837088388 24.003466204506065 82-83 22.660311958405547 23.310225303292896 29.809358752166375 24.220103986135182 84-85 21.447140381282495 25.99653379549394 26.51646447140381 26.03986135181976 86-87 20.92720970537262 25.736568457538993 27.55632582322357 25.779896013864818 88-89 23.440207972270365 23.39688041594454 27.209705372616984 25.953206239168107 90-91 24.653379549393414 23.873483535528596 25.0 26.473136915077987 92-93 24.826689774696707 23.483535528596185 24.783362218370883 26.90641247833622 94-95 24.696707105719238 21.273830155979205 27.859618717504333 26.16984402079723 96-97 26.8630849220104 20.27729636048527 26.819757365684577 26.03986135181976 98-99 25.433275563258235 20.84055459272097 27.29636048526863 26.429809358752166 100-101 25.17331022530329 23.180242634315423 26.993067590987867 24.653379549393414 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.5 2 1.0 3 0.5 4 0.0 5 1.0 6 2.0 7 2.0 8 2.0 9 6.0 10 7.0 11 5.0 12 4.5 13 5.0 14 4.5 15 2.5 16 1.5 17 2.0 18 6.5 19 6.5 20 4.5 21 7.0 22 8.0 23 8.0 24 7.0 25 7.0 26 11.5 27 12.5 28 12.5 29 14.5 30 12.0 31 10.0 32 12.5 33 17.0 34 20.0 35 18.0 36 15.5 37 16.5 38 21.0 39 27.5 40 28.0 41 32.5 42 34.5 43 31.0 44 29.0 45 35.5 46 44.0 47 41.0 48 48.5 49 51.5 50 49.0 51 46.0 52 48.0 53 49.5 54 42.5 55 36.5 56 25.5 57 22.5 58 22.0 59 17.0 60 17.5 61 12.5 62 10.0 63 13.5 64 9.0 65 4.5 66 5.5 67 6.5 68 5.0 69 3.0 70 2.0 71 2.5 72 2.5 73 2.0 74 1.0 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25929127052722556 2 0.25929127052722556 3 0.25929127052722556 4 0.34572169403630076 5 0.25929127052722556 6 0.25929127052722556 7 0.25929127052722556 8 0.25929127052722556 9 0.25929127052722556 10-11 0.25929127052722556 12-13 0.25929127052722556 14-15 0.25929127052722556 16-17 0.25929127052722556 18-19 0.25929127052722556 20-21 0.25929127052722556 22-23 0.25929127052722556 24-25 0.25929127052722556 26-27 0.25929127052722556 28-29 0.25929127052722556 30-31 0.25929127052722556 32-33 0.25929127052722556 34-35 0.17286084701815038 36-37 0.25929127052722556 38-39 0.25929127052722556 40-41 0.25929127052722556 42-43 0.25929127052722556 44-45 0.25929127052722556 46-47 0.25929127052722556 48-49 0.25929127052722556 50-51 0.216076058772688 52-53 0.216076058772688 54-55 0.25929127052722556 56-57 0.25929127052722556 58-59 0.25929127052722556 60-61 0.25929127052722556 62-63 0.25929127052722556 64-65 0.25929127052722556 66-67 0.25929127052722556 68-69 0.25929127052722556 70-71 0.25929127052722556 72-73 0.25929127052722556 74-75 0.25929127052722556 76-77 0.25929127052722556 78-79 0.25929127052722556 80-81 0.25929127052722556 82-83 0.25929127052722556 84-85 0.25929127052722556 86-87 0.25929127052722556 88-89 0.25929127052722556 90-91 0.25929127052722556 92-93 0.25929127052722556 94-95 0.25929127052722556 96-97 0.25929127052722556 98-99 0.25929127052722556 100-101 0.25929127052722556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1157.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.03630077787382 #Duplication Level Percentage of deduplicated Percentage of total 1 98.89705882352942 92.9991356957649 2 0.6433823529411764 1.2100259291270528 3 0.0 0.0 4 0.09191176470588235 0.34572169403630076 5 0.0 0.0 6 0.0 0.0 7 0.09191176470588235 0.6050129645635264 8 0.0 0.0 9 0.0 0.0 >10 0.2757352941176471 4.840103716508211 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31 2.679343128781331 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 1.2100259291270528 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.9507346585998271 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.6050129645635264 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.34572169403630076 No Hit TTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAACAAC 2 0.17286084701815038 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2 0.17286084701815038 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.17286084701815038 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 2 0.17286084701815038 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 2 0.17286084701815038 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.17286084701815038 No Hit GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.17286084701815038 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.08643042350907519 0.0 26-27 0.0 0.0 0.0 0.25929127052722556 0.0 28-29 0.0 0.0 0.0 1.0371650821089022 0.0 30-31 0.0 0.0 0.0 2.33362143474503 0.0 32-33 0.0 0.0 0.0 2.981849611063094 0.0 34-35 0.0 0.0 0.0 3.846153846153846 0.0 36-37 0.0 0.0 0.0 5.4019014693172 0.0 38-39 0.0 0.0 0.0 7.389801210025929 0.0 40-41 0.0 0.0 0.0 9.075194468452896 0.0 42-43 0.0 0.0 0.0 10.458081244598098 0.0 44-45 0.0 0.0 0.0 10.97666378565255 0.0 46-47 0.0 0.0 0.0 11.408815903197926 0.0 48-49 0.0 0.0 0.0 11.840968020743302 0.0 50-51 0.0 0.0 0.0 12.359550561797754 0.0 52-53 0.0 0.0 0.0 13.569576490924806 0.0 54-55 0.0 0.0 0.0 14.00172860847018 0.0 56-57 0.0 0.0 0.0 15.773552290406222 0.0 58-59 0.0 0.0 0.0 17.588591184096803 0.0 60-61 0.0 0.0 0.0 18.625756266205705 0.0 62-63 0.0 0.0 0.0 19.576490924805533 0.0 64-65 0.0 0.0 0.0 22.169403630077788 0.0 66-67 0.0 0.0 0.0 24.070872947277444 0.0 68-69 0.0 0.0 0.0 24.589455488331893 0.0 70-71 0.0 0.0 0.0 26.57735522904062 0.0 72-73 0.0 0.0 0.0 29.29991356957649 0.0 74-75 0.0 0.0 0.0 30.639585133967156 0.0 76-77 0.0 0.0 0.0 31.67675021607606 0.0 78-79 0.0 0.0 0.0 32.67070008643043 0.0 80-81 0.0 0.0 0.0 33.4485738980121 0.0 82-83 0.0 0.0 0.0 33.62143474503025 0.0 84-85 0.0 0.0 0.0 33.62143474503025 0.0 86-87 0.0 0.0 0.0 33.70786516853933 0.0 88-89 0.0 0.0 0.0 33.70786516853933 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE