FastQCFastQC Report
Thu 26 May 2016
SRR937293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1157
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211.8150388936905792No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT151.296456352636128RNA PCR Primer, Index 33 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT121.0371650821089022No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT121.0371650821089022RNA PCR Primer, Index 23 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT121.0371650821089022RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121.0371650821089022No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT121.0371650821089022No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG110.9507346585998271RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG110.9507346585998271RNA PCR Primer, Index 33 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT100.864304235090752No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT90.7778738115816767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.7778738115816767No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT80.6914433880726015No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT80.6914433880726015TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT70.6050129645635264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.6050129645635264No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG70.6050129645635264RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT60.5185825410544511RNA PCR Primer, Index 23 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC60.5185825410544511No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT50.432152117545376RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT50.432152117545376No Hit
GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA50.432152117545376No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG50.432152117545376TruSeq Adapter, Index 1 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT50.432152117545376TruSeq Adapter, Index 1 (95% over 21bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG40.34572169403630076RNA PCR Primer, Index 39 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC40.34572169403630076No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT40.34572169403630076RNA PCR Primer, Index 23 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT40.34572169403630076RNA PCR Primer, Index 27 (96% over 25bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA40.34572169403630076RNA PCR Primer, Index 33 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.34572169403630076No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC40.34572169403630076No Hit
CATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAA40.34572169403630076No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT40.34572169403630076No Hit
GTCCTAGACTGTGGTAGAGTCCATGATTGAGGTTCCTGGGACATTCCCAG40.34572169403630076No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT30.25929127052722556TruSeq Adapter, Index 11 (95% over 21bp)
CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA30.25929127052722556No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA30.25929127052722556RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT30.25929127052722556RNA PCR Primer, Index 31 (95% over 24bp)
GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG30.25929127052722556No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.25929127052722556No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG30.25929127052722556Illumina PCR Primer Index 1 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT30.25929127052722556RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT30.25929127052722556No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT30.25929127052722556RNA PCR Primer, Index 31 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT30.25929127052722556RNA PCR Primer, Index 3 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT30.25929127052722556RNA PCR Primer, Index 46 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT30.25929127052722556No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT30.25929127052722556RNA PCR Primer, Index 33 (96% over 27bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG30.25929127052722556RNA PCR Primer, Index 27 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGTCTT20.17286084701815038No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT20.17286084701815038RNA PCR Primer, Index 46 (95% over 23bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC20.17286084701815038No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT20.17286084701815038RNA PCR Primer, Index 1 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT20.17286084701815038RNA PCR Primer, Index 33 (95% over 24bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT20.17286084701815038RNA PCR Primer, Index 31 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC20.17286084701815038No Hit
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC20.17286084701815038No Hit
TCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA20.17286084701815038RNA PCR Primer, Index 38 (96% over 28bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG20.17286084701815038RNA PCR Primer, Index 26 (100% over 29bp)
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT20.17286084701815038RNA PCR Primer, Index 27 (95% over 22bp)
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG20.17286084701815038No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT20.17286084701815038No Hit
CCCATGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGC20.17286084701815038No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG20.17286084701815038RNA PCR Primer, Index 26 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT20.17286084701815038No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA20.17286084701815038RNA PCR Primer, Index 3 (96% over 28bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT20.17286084701815038RNA PCR Primer, Index 39 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT20.17286084701815038TruSeq Adapter, Index 1 (96% over 26bp)
GCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACT20.17286084701815038No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC20.17286084701815038No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers