##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937293_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1157 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.11408815903198 31.0 30.0 31.0 26.0 34.0 2 30.26361279170268 31.0 30.0 31.0 27.0 34.0 3 30.364736387208296 31.0 30.0 31.0 27.0 34.0 4 34.315471045808124 35.0 35.0 37.0 30.0 37.0 5 33.99654278305964 35.0 33.0 37.0 30.0 37.0 6 33.774416594641316 35.0 33.0 37.0 29.0 37.0 7 33.51080380293863 35.0 33.0 37.0 29.0 37.0 8 33.37942955920484 35.0 32.0 37.0 28.0 37.0 9 34.81763180639585 37.0 33.0 39.0 28.0 39.0 10-11 34.8781331028522 37.0 33.0 39.0 29.0 39.0 12-13 34.46542783059637 37.0 32.5 39.0 27.0 39.0 14-15 35.01771823681936 37.0 32.5 39.0 27.0 40.0 16-17 34.66637856525497 36.5 32.0 39.0 27.0 40.0 18-19 35.052722558340534 37.0 33.0 39.0 27.0 40.0 20-21 35.09161624891962 37.0 33.0 39.0 27.0 40.0 22-23 34.652981849611066 36.0 32.5 39.0 26.5 40.0 24-25 34.1875540190147 36.0 32.0 39.0 25.0 40.0 26-27 33.78522039757995 36.0 31.5 39.0 24.5 40.0 28-29 32.88807260155575 35.0 30.0 38.5 23.0 40.0 30-31 32.906655142610205 35.0 30.5 38.0 23.5 40.0 32-33 32.71305099394987 35.0 30.0 38.0 23.0 40.0 34-35 31.999567847882453 35.0 30.0 38.0 20.5 40.0 36-37 31.45980985306828 35.0 30.0 38.0 17.5 40.0 38-39 30.73552290406223 34.5 28.5 38.0 14.0 40.0 40-41 30.487899740708727 34.0 27.5 38.0 14.0 39.5 42-43 30.40449438202247 34.0 27.5 38.0 9.0 40.0 44-45 29.763180639585137 34.0 26.5 38.0 4.5 40.0 46-47 28.772687986171135 33.5 24.5 37.5 2.0 40.0 48-49 28.421348314606742 33.0 24.5 38.0 2.0 40.0 50-51 27.417026793431287 33.0 23.0 37.0 2.0 39.0 52-53 26.796888504753674 33.0 21.0 37.0 2.0 39.0 54-55 25.643906655142608 32.0 13.0 36.5 2.0 39.0 56-57 24.037165082108903 31.0 2.0 36.0 2.0 38.5 58-59 22.75151253241141 30.0 2.0 35.0 2.0 38.0 60-61 21.405790838375108 28.0 2.0 35.0 2.0 38.0 62-63 19.655142610198787 26.0 2.0 34.0 2.0 38.0 64-65 18.17631806395851 21.0 2.0 34.0 2.0 37.0 66-67 16.814606741573034 9.0 2.0 33.0 2.0 37.0 68-69 16.21866897147796 2.0 2.0 32.5 2.0 36.0 70-71 15.910112359550563 2.0 2.0 32.5 2.0 36.0 72-73 15.381158167675022 2.0 2.0 32.0 2.0 35.0 74-75 15.153846153846153 2.0 2.0 31.5 2.0 35.0 76-77 14.156006914433881 2.0 2.0 29.5 2.0 34.0 78-79 14.486171132238548 2.0 2.0 31.0 2.0 34.5 80-81 14.226879861711321 2.0 2.0 31.0 2.0 34.5 82-83 13.633967156439066 2.0 2.0 30.5 2.0 34.0 84-85 13.100259291270527 2.0 2.0 30.0 2.0 34.0 86-87 12.676318063958513 2.0 2.0 29.0 2.0 34.0 88-89 12.207865168539326 2.0 2.0 29.0 2.0 34.0 90-91 11.56266205704408 2.0 2.0 27.0 2.0 33.5 92-93 10.949006050129647 2.0 2.0 26.0 2.0 33.0 94-95 10.332324978392393 2.0 2.0 24.0 2.0 33.0 96-97 9.705272255834053 2.0 2.0 20.0 2.0 32.5 98-99 9.192307692307693 2.0 2.0 18.0 2.0 32.0 100-101 8.56352636127917 2.0 2.0 2.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.4816849816849818 1101 2 3.5073260073260073 1101 3 3.756410256410259 1101 4 2.8681318681318686 1101 5 3.23260073260073 1101 6 2.8992673992674014 1101 7 3.42307692307692 1101 8 3.5054945054945037 1101 9 4.23260073260073 1101 10-11 3.3818681318681314 1101 12-13 3.426739926739927 1101 14-15 3.724358974358971 1101 16-17 6.456959706959708 1101 18-19 5.987179487179489 1101 20-21 5.6575091575091605 1101 22-23 5.802197802197803 1101 24-25 4.100732600732599 1101 26-27 0.0650183150183139 1101 28-29 6.207875457875453 1101 30-31 3.7902930402930366 1101 32-33 6.1098901098901095 1101 34-35 7.1272893772893795 1101 36-37 6.733516483516482 1101 38-39 -0.4450549450549417 1101 40-41 -4.736263736263737 1101 42-43 -11.573260073260073 1101 44-45 -4.839743589743588 1101 46-47 1.6996336996336971 1101 48-49 5.184065934065934 1101 50-51 5.468864468864467 1101 52-53 5.310439560439562 1101 54-55 8.63827838827839 1101 56-57 -2.7289377289377263 1101 58-59 -8.615384615384617 1101 60-61 -22.915750915750912 1101 62-63 -21.560439560439562 1101 64-65 -19.42216117216117 1101 66-67 -17.824175824175825 1101 68-69 -16.445054945054945 1101 70-71 -16.990842490842493 1101 72-73 -16.10164835164835 1101 74-75 -15.593406593406595 1101 76-77 -15.052197802197803 1101 78-79 -15.241758241758241 1101 80-81 -14.032967032967033 1101 82-83 -13.557692307692308 1101 84-85 -12.961538461538462 1101 86-87 -12.535714285714286 1101 88-89 -12.31868131868132 1101 90-91 -10.851648351648352 1101 92-93 -10.56868131868132 1101 94-95 -10.12087912087912 1101 96-97 -9.434065934065934 1101 98-99 -8.208791208791208 1101 100-101 -8.024725274725276 1103 1 0.48168498168498175 1103 2 0.5073260073260073 1103 3 -1.243589743589741 1103 4 0.8681318681318686 1103 5 -0.26739926739927 1103 6 1.3992673992674014 1103 7 2.42307692307692 1103 8 -0.4945054945054963 1103 9 2.23260073260073 1103 10-11 0.3818681318681314 1103 12-13 2.426739926739927 1103 14-15 -0.2756410256410291 1103 16-17 1.206959706959708 1103 18-19 -1.762820512820511 1103 20-21 2.1575091575091605 1103 22-23 1.302197802197803 1103 24-25 -1.1492673992674014 1103 26-27 -0.6849816849816861 1103 28-29 -2.5421245421245473 1103 30-31 3.2902930402930366 1103 32-33 4.8598901098901095 1103 34-35 4.3772893772893795 1103 36-37 -2.016483516483518 1103 38-39 4.054945054945058 1103 40-41 2.513736263736263 1103 42-43 3.926739926739927 1103 44-45 -2.3397435897435876 1103 46-47 -5.550366300366303 1103 48-49 3.1840659340659343 1103 50-51 -10.031135531135533 1103 52-53 -7.939560439560438 1103 54-55 -9.61172161172161 1103 56-57 -8.978937728937726 1103 58-59 -8.615384615384617 1103 60-61 -7.9157509157509125 1103 62-63 -11.560439560439562 1103 64-65 -15.172161172161172 1103 66-67 -5.824175824175825 1103 68-69 -2.4450549450549453 1103 70-71 -0.9908424908424927 1103 72-73 -9.35164835164835 1103 74-75 -6.093406593406595 1103 76-77 -4.302197802197803 1103 78-79 -3.241758241758241 1103 80-81 0.7170329670329672 1103 82-83 1.9423076923076916 1103 84-85 2.5384615384615383 1103 86-87 2.9642857142857135 1103 88-89 1.1813186813186807 1103 90-91 -10.851648351648352 1103 92-93 -10.56868131868132 1103 94-95 -10.12087912087912 1103 96-97 -9.434065934065934 1103 98-99 -8.208791208791208 1103 100-101 -8.024725274725276 1104 1 0.48168498168498175 1104 2 -3.4926739926739927 1104 3 -2.243589743589741 1104 4 -4.131868131868131 1104 5 1.23260073260073 1104 6 0.8992673992674014 1104 7 3.42307692307692 1104 8 -0.4945054945054963 1104 9 4.23260073260073 1104 10-11 1.8818681318681314 1104 12-13 3.926739926739927 1104 14-15 4.224358974358971 1104 16-17 -4.043040293040292 1104 18-19 4.487179487179489 1104 20-21 3.6575091575091605 1104 22-23 3.302197802197803 1104 24-25 2.1007326007325986 1104 26-27 3.565018315018314 1104 28-29 3.2078754578754527 1104 30-31 0.2902930402930366 1104 32-33 5.1098901098901095 1104 34-35 -4.3727106227106205 1104 36-37 1.2335164835164818 1104 38-39 4.554945054945058 1104 40-41 -0.7362637362637372 1104 42-43 -2.573260073260073 1104 44-45 2.6602564102564124 1104 46-47 7.199633699633697 1104 48-49 7.184065934065934 1104 50-51 5.968864468864467 1104 52-53 10.310439560439562 1104 54-55 7.6382783882783905 1104 56-57 8.271062271062274 1104 58-59 10.884615384615383 1104 60-61 10.584249084249088 1104 62-63 6.939560439560438 1104 64-65 12.577838827838828 1104 66-67 16.675824175824175 1104 68-69 8.554945054945055 1104 70-71 12.509157509157507 1104 72-73 15.89835164835165 1104 74-75 16.406593406593405 1104 76-77 15.447802197802197 1104 78-79 14.758241758241759 1104 80-81 7.467032967032967 1104 82-83 3.4423076923076916 1104 84-85 16.53846153846154 1104 86-87 15.464285714285714 1104 88-89 15.68131868131868 1104 90-91 7.648351648351648 1104 92-93 1.4313186813186807 1104 94-95 -1.1208791208791204 1104 96-97 19.065934065934066 1104 98-99 -8.208791208791208 1104 100-101 -8.024725274725276 1105 1 0.48168498168498175 1105 2 0.5073260073260073 1105 3 -0.24358974358974095 1105 4 0.8681318681318686 1105 5 1.23260073260073 1105 6 0.8992673992674014 1105 7 -1.5769230769230802 1105 8 -4.494505494505496 1105 9 2.23260073260073 1105 10-11 0.8818681318681314 1105 12-13 -3.573260073260073 1105 14-15 -0.7756410256410291 1105 16-17 0.956959706959708 1105 18-19 2.987179487179489 1105 20-21 -1.8424908424908395 1105 22-23 -0.6978021978021971 1105 24-25 -2.8992673992674014 1105 26-27 2.565018315018314 1105 28-29 5.707875457875453 1105 30-31 5.790293040293037 1105 32-33 3.1098901098901095 1105 34-35 -1.3727106227106205 1105 36-37 0.7335164835164818 1105 38-39 6.554945054945058 1105 40-41 2.263736263736263 1105 42-43 -18.573260073260073 1105 44-45 -15.339743589743588 1105 46-47 1.6996336996336971 1105 48-49 5.684065934065934 1105 50-51 2.968864468864467 1105 52-53 0.810439560439562 1105 54-55 4.6382783882783905 1105 56-57 4.771062271062274 1105 58-59 6.384615384615383 1105 60-61 -4.9157509157509125 1105 62-63 -13.560439560439562 1105 64-65 -1.9221611721611715 1105 66-67 10.675824175824175 1105 68-69 9.054945054945055 1105 70-71 9.009157509157507 1105 72-73 -1.1016483516483504 1105 74-75 -3.593406593406595 1105 76-77 10.447802197802197 1105 78-79 12.758241758241759 1105 80-81 14.967032967032967 1105 82-83 0.4423076923076916 1105 84-85 5.038461538461538 1105 86-87 3.9642857142857135 1105 88-89 11.68131868131868 1105 90-91 -2.851648351648352 1105 92-93 8.43131868131868 1105 94-95 17.87912087912088 1105 96-97 21.065934065934066 1105 98-99 13.791208791208792 1105 100-101 14.475274725274724 1107 1 1.4816849816849818 1107 2 1.5073260073260073 1107 3 1.756410256410259 1107 4 1.8681318681318686 1107 5 2.23260073260073 1107 6 1.8992673992674014 1107 7 -7.57692307692308 1107 8 -7.494505494505496 1107 9 0.23260073260073 1107 10-11 1.1318681318681314 1107 12-13 0.926739926739927 1107 14-15 -1.525641025641029 1107 16-17 -4.543040293040292 1107 18-19 2.737179487179489 1107 20-21 -3.0924908424908395 1107 22-23 0.8021978021978029 1107 24-25 2.8507326007325986 1107 26-27 -3.434981684981686 1107 28-29 -8.042124542124547 1107 30-31 -0.7097069597069634 1107 32-33 -3.6401098901098905 1107 34-35 0.6272893772893795 1107 36-37 1.9835164835164818 1107 38-39 -6.195054945054942 1107 40-41 -2.236263736263737 1107 42-43 2.426739926739927 1107 44-45 1.6602564102564124 1107 46-47 6.449633699633697 1107 48-49 -9.315934065934066 1107 50-51 -8.281135531135533 1107 52-53 -8.439560439560438 1107 54-55 -8.11172161172161 1107 56-57 -8.478937728937726 1107 58-59 -8.115384615384617 1107 60-61 -13.915750915750912 1107 62-63 -9.810439560439562 1107 64-65 -3.9221611721611715 1107 66-67 -10.074175824175825 1107 68-69 -16.445054945054945 1107 70-71 -16.990842490842493 1107 72-73 -16.10164835164835 1107 74-75 -15.593406593406595 1107 76-77 -15.052197802197803 1107 78-79 -15.241758241758241 1107 80-81 -14.032967032967033 1107 82-83 -13.557692307692308 1107 84-85 -12.961538461538462 1107 86-87 -12.535714285714286 1107 88-89 -12.31868131868132 1107 90-91 -10.851648351648352 1107 92-93 -10.56868131868132 1107 94-95 -10.12087912087912 1107 96-97 -9.434065934065934 1107 98-99 -8.208791208791208 1107 100-101 -8.024725274725276 1108 1 3.4816849816849818 1108 2 3.5073260073260073 1108 3 3.756410256410259 1108 4 2.8681318681318686 1108 5 3.23260073260073 1108 6 2.8992673992674014 1108 7 3.42307692307692 1108 8 3.5054945054945037 1108 9 4.23260073260073 1108 10-11 3.8818681318681314 1108 12-13 4.426739926739927 1108 14-15 4.224358974358971 1108 16-17 5.956959706959708 1108 18-19 5.987179487179489 1108 20-21 5.6575091575091605 1108 22-23 6.302197802197803 1108 24-25 6.600732600732599 1108 26-27 6.065018315018314 1108 28-29 8.207875457875453 1108 30-31 7.790293040293037 1108 32-33 7.6098901098901095 1108 34-35 8.12728937728938 1108 36-37 8.233516483516482 1108 38-39 8.054945054945058 1108 40-41 8.263736263736263 1108 42-43 9.426739926739927 1108 44-45 9.160256410256412 1108 46-47 10.199633699633697 1108 48-49 10.184065934065934 1108 50-51 11.968864468864467 1108 52-53 13.310439560439562 1108 54-55 11.63827838827839 1108 56-57 12.771062271062274 1108 58-59 15.884615384615383 1108 60-61 12.584249084249088 1108 62-63 13.939560439560438 1108 64-65 10.577838827838828 1108 66-67 17.175824175824175 1108 68-69 18.554945054945055 1108 70-71 18.009157509157507 1108 72-73 18.39835164835165 1108 74-75 18.406593406593405 1108 76-77 18.947802197802197 1108 78-79 18.75824175824176 1108 80-81 19.467032967032967 1108 82-83 18.942307692307693 1108 84-85 20.03846153846154 1108 86-87 20.464285714285715 1108 88-89 20.68131868131868 1108 90-91 22.14835164835165 1108 92-93 21.43131868131868 1108 94-95 21.87912087912088 1108 96-97 23.565934065934066 1108 98-99 24.791208791208792 1108 100-101 23.975274725274723 1109 1 0.48168498168498175 1109 2 0.5073260073260073 1109 3 3.756410256410259 1109 4 0.8681318681318686 1109 5 1.23260073260073 1109 6 0.8992673992674014 1109 7 3.42307692307692 1109 8 1.5054945054945037 1109 9 4.23260073260073 1109 10-11 0.3818681318681314 1109 12-13 -1.073260073260073 1109 14-15 3.724358974358971 1109 16-17 2.956959706959708 1109 18-19 0.487179487179489 1109 20-21 1.1575091575091605 1109 22-23 -3.197802197802197 1109 24-25 1.6007326007325986 1109 26-27 0.0650183150183139 1109 28-29 -10.792124542124547 1109 30-31 -3.2097069597069634 1109 32-33 3.1098901098901095 1109 34-35 -11.87271062271062 1109 36-37 -20.766483516483518 1109 38-39 -0.9450549450549417 1109 40-41 -14.736263736263737 1109 42-43 -6.073260073260073 1109 44-45 4.660256410256412 1109 46-47 -6.800366300366303 1109 48-49 -7.315934065934066 1109 50-51 1.4688644688644672 1109 52-53 -12.689560439560438 1109 54-55 6.1382783882783905 1109 56-57 -6.228937728937726 1109 58-59 -23.115384615384617 1109 60-61 -22.915750915750912 1109 62-63 -21.560439560439562 1109 64-65 -19.42216117216117 1109 66-67 -17.824175824175825 1109 68-69 -16.445054945054945 1109 70-71 -16.990842490842493 1109 72-73 -16.10164835164835 1109 74-75 -15.593406593406595 1109 76-77 -15.052197802197803 1109 78-79 -15.241758241758241 1109 80-81 -14.032967032967033 1109 82-83 -13.557692307692308 1109 84-85 -12.961538461538462 1109 86-87 -12.535714285714286 1109 88-89 -12.31868131868132 1109 90-91 -10.851648351648352 1109 92-93 -10.56868131868132 1109 94-95 -10.12087912087912 1109 96-97 -9.434065934065934 1109 98-99 -8.208791208791208 1109 100-101 -8.024725274725276 1110 1 -1.5183150183150182 1110 2 0.0073260073260073 1110 3 0.756410256410259 1110 4 -0.1318681318681314 1110 5 1.23260073260073 1110 6 0.8992673992674014 1110 7 -0.5769230769230802 1110 8 2.5054945054945037 1110 9 3.23260073260073 1110 10-11 -1.6181318681318686 1110 12-13 -0.073260073260073 1110 14-15 -2.775641025641029 1110 16-17 -6.043040293040292 1110 18-19 1.987179487179489 1110 20-21 0.6575091575091605 1110 22-23 -2.447802197802197 1110 24-25 -4.149267399267401 1110 26-27 -5.434981684981686 1110 28-29 -0.7921245421245473 1110 30-31 -10.459706959706963 1110 32-33 -1.6401098901098905 1110 34-35 -0.3727106227106205 1110 36-37 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1.9816849816849818 1111 2 3.5073260073260073 1111 3 2.256410256410259 1111 4 2.8681318681318686 1111 5 3.23260073260073 1111 6 2.8992673992674014 1111 7 3.42307692307692 1111 8 3.0054945054945037 1111 9 3.23260073260073 1111 10-11 3.8818681318681314 1111 12-13 3.926739926739927 1111 14-15 5.224358974358971 1111 16-17 5.456959706959708 1111 18-19 3.987179487179489 1111 20-21 2.4075091575091605 1111 22-23 3.802197802197803 1111 24-25 4.850732600732599 1111 26-27 3.065018315018314 1111 28-29 2.7078754578754527 1111 30-31 6.290293040293037 1111 32-33 6.3598901098901095 1111 34-35 4.3772893772893795 1111 36-37 6.983516483516482 1111 38-39 6.554945054945058 1111 40-41 2.763736263736263 1111 42-43 6.176739926739927 1111 44-45 6.910256410256412 1111 46-47 2.949633699633697 1111 48-49 5.684065934065934 1111 50-51 6.218864468864467 1111 52-53 9.060439560439562 1111 54-55 7.6382783882783905 1111 56-57 9.521062271062274 1111 58-59 -4.115384615384617 1111 60-61 -4.9157509157509125 1111 62-63 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3.42307692307692 2316 8 3.5054945054945037 2316 9 3.23260073260073 2316 10-11 3.1318681318681314 2316 12-13 4.176739926739927 2316 14-15 4.974358974358971 2316 16-17 6.206959706959708 2316 18-19 5.487179487179489 2316 20-21 2.9075091575091605 2316 22-23 5.552197802197803 2316 24-25 5.600732600732599 2316 26-27 6.065018315018314 2316 28-29 7.207875457875453 2316 30-31 6.540293040293037 2316 32-33 4.6098901098901095 2316 34-35 5.6272893772893795 2316 36-37 6.483516483516482 2316 38-39 6.804945054945058 2316 40-41 6.263736263736263 2316 42-43 7.426739926739927 2316 44-45 7.660256410256412 2316 46-47 8.949633699633697 2316 48-49 4.934065934065934 2316 50-51 8.968864468864467 2316 52-53 8.810439560439562 2316 54-55 11.38827838827839 2316 56-57 14.021062271062274 2316 58-59 13.884615384615383 2316 60-61 14.584249084249088 2316 62-63 12.439560439560438 2316 64-65 14.577838827838828 2316 66-67 13.425824175824175 2316 68-69 18.804945054945055 2316 70-71 16.759157509157507 2316 72-73 20.39835164835165 2316 74-75 18.906593406593405 2316 76-77 18.197802197802197 2316 78-79 20.25824175824176 2316 80-81 19.717032967032967 2316 82-83 19.692307692307693 2316 84-85 21.03846153846154 2316 86-87 18.964285714285715 2316 88-89 20.93131868131868 2316 90-91 21.64835164835165 2316 92-93 18.68131868131868 2316 94-95 13.87912087912088 2316 96-97 14.315934065934066 2316 98-99 19.041208791208792 2316 100-101 21.225274725274723 >>END_MODULE >>Per sequence quality scores fail #Quality Count 6 2.0 7 3.0 8 5.0 9 9.0 10 17.0 11 14.0 12 23.0 13 24.0 14 36.0 15 37.0 16 49.0 17 42.0 18 64.0 19 68.0 20 64.0 21 50.0 22 51.0 23 49.0 24 51.0 25 40.0 26 25.0 27 38.0 28 32.0 29 43.0 30 42.0 31 40.0 32 51.0 33 42.0 34 44.0 35 42.0 36 28.0 37 19.0 38 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.682800345721695 25.49697493517718 18.409680207433016 25.41054451166811 2 10.544511668107173 23.16335350043215 44.2523768366465 22.039757994814174 3 14.261019878997407 32.4114088159032 37.77009507346586 15.557476231633535 4 8.815903197925671 26.01555747623163 27.225583405358687 37.94295592048401 5 7.605877268798617 41.31374243733794 33.36214347450303 17.718236819360413 6 20.743301642178047 39.15298184961106 15.730337078651685 24.373379429559204 7 16.248919619706136 43.2152117545376 15.557476231633535 24.978392394122732 8 21.34831460674157 35.609334485738984 21.6076058772688 21.43474503025065 9 16.16248919619706 19.101123595505616 16.594641313742436 48.14174589455488 10-11 16.94036300777874 28.13310285220398 26.879861711322384 28.0466724286949 12-13 22.904062229904927 26.05877268798617 22.904062229904927 28.13310285220398 14-15 22.731201382886777 30.85566119273985 18.53932584269663 27.87381158167675 16-17 20.613656006914432 35.04753673292999 19.05790838375108 25.280898876404496 18-19 18.75540190146932 28.867761452031115 23.768366464995676 28.608470181503886 20-21 19.922212618841833 28.3923941227312 23.811581676750215 27.87381158167675 22-23 21.650821089023335 33.664649956784785 23.72515125324114 20.959377700950736 24-25 22.817631806395852 34.78824546240277 23.768366464995676 18.625756266205702 26-27 19.74935177182368 35.782195332757134 26.188418323249785 18.280034572169406 28-29 18.020743301642177 31.244598098530684 27.52808988764045 23.20656871218669 30-31 22.687986171132238 28.99740708729473 29.64563526361279 18.66897147796024 32-33 20.570440795159897 32.02247191011236 29.99135695764909 17.415730337078653 34-35 16.94036300777874 32.02247191011236 33.664649956784785 17.372515125324114 36-37 16.076058772687986 30.682800345721695 30.121002592912703 23.120138288677612 38-39 18.366464995678477 25.71305099394987 31.46067415730337 24.45980985306828 40-41 14.736387208297321 27.225583405358687 34.57216940363008 23.465859982713916 42-43 19.57649092480553 24.805531547104582 37.035436473638725 18.582541054451166 44-45 14.909248055315471 26.44770959377701 34.831460674157306 23.811581676750215 46-47 21.00259291270527 22.817631806395852 29.86171132238548 26.3180639585134 48-49 17.329299913569578 24.589455488331893 32.800345721694036 25.280898876404496 50-51 17.329299913569578 27.441659464131373 32.238547968885044 22.990492653414 52-53 16.292134831460675 29.559204840103714 34.57216940363008 19.57649092480553 54-55 15.816767502160761 29.515989628349175 30.423509075194467 24.24373379429559 56-57 19.144338807260155 31.676750216076062 25.972342264477096 23.20656871218669 58-59 16.810717372515125 35.22039757994814 30.337078651685395 17.631806395851342 60-61 18.452895419187556 41.87554019014693 22.169403630077788 17.502160760587728 62-63 14.822817631806394 43.171996542783056 20.916162489196196 21.089023336214346 64-65 13.223854796888505 41.054451166810715 23.422644770959376 22.2990492653414 66-67 16.076058772687986 37.381158167675025 25.66983578219533 20.872947277441657 68-69 16.76750216076059 36.99222126188418 24.200518582541054 22.039757994814174 70-71 16.33535004321521 33.83751080380294 24.978392394122732 24.848746758859118 72-73 18.71218668971478 30.466724286949002 27.744165946413133 23.076923076923077 74-75 17.588591184096803 30.337078651685395 27.7009507346586 24.373379429559204 76-77 16.8971477960242 28.82454624027658 26.879861711322384 27.398444252376837 78-79 17.97752808988764 29.34312878133103 26.27484874675886 26.40449438202247 80-81 18.236819360414867 28.910976663785654 27.441659464131373 25.41054451166811 82-83 17.28608470181504 27.355229040622298 26.6637856525497 28.69490060501296 84-85 18.236819360414867 28.13310285220398 23.638720829732065 29.99135695764909 86-87 19.403630077787383 29.12705272255834 26.05877268798617 25.41054451166811 88-89 19.403630077787383 28.3923941227312 24.373379429559204 27.830596369922212 90-91 18.71218668971478 26.96629213483146 22.817631806395852 31.50388936905791 92-93 22.6447709593777 28.954191875540193 22.515125324114088 25.885911840968024 94-95 23.292999135695766 27.398444252376837 22.082973206568713 27.225583405358687 96-97 21.04580812445981 28.608470181503886 22.428694900605013 27.917026793431287 98-99 20.829732065687125 28.651685393258425 22.2990492653414 28.219533275713047 100-101 23.16335350043215 32.15211754537597 21.73725151253241 22.947277441659462 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 1.5 3 2.5 4 1.0 5 1.0 6 1.5 7 3.5 8 5.5 9 4.5 10 4.5 11 3.5 12 3.5 13 4.5 14 3.5 15 2.5 16 3.0 17 4.0 18 4.0 19 3.5 20 2.0 21 4.5 22 7.5 23 6.0 24 7.5 25 10.0 26 8.0 27 8.0 28 12.5 29 9.5 30 6.0 31 12.5 32 16.0 33 13.0 34 11.0 35 14.5 36 21.5 37 22.0 38 22.0 39 25.5 40 25.5 41 31.5 42 44.0 43 52.0 44 55.0 45 59.0 46 61.0 47 60.5 48 62.5 49 63.5 50 64.5 51 59.0 52 46.5 53 40.5 54 36.0 55 31.0 56 24.5 57 14.5 58 7.5 59 5.5 60 5.5 61 4.5 62 2.5 63 1.5 64 1.0 65 0.0 66 0.5 67 0.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1157.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.05877268798618 #Duplication Level Percentage of deduplicated Percentage of total 1 92.04545454545455 70.00864304235091 2 2.3863636363636367 3.6300777873811585 3 1.7045454545454544 3.8893690579083837 4 1.1363636363636365 3.457216940363008 5 0.5681818181818182 2.16076058772688 6 0.22727272727272727 1.0371650821089022 7 0.3409090909090909 1.8150388936905792 8 0.22727272727272727 1.382886776145203 9 0.22727272727272727 1.5557476231633534 >10 1.1363636363636365 11.063094209161624 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 1.8150388936905792 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 15 1.296456352636128 RNA PCR Primer, Index 33 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 12 1.0371650821089022 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 12 1.0371650821089022 RNA PCR Primer, Index 23 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 12 1.0371650821089022 RNA PCR Primer, Index 31 (95% over 22bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 1.0371650821089022 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 12 1.0371650821089022 No Hit TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG 11 0.9507346585998271 RNA PCR Primer, Index 31 (96% over 29bp) TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 11 0.9507346585998271 RNA PCR Primer, Index 33 (96% over 29bp) CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 10 0.864304235090752 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 9 0.7778738115816767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.7778738115816767 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 8 0.6914433880726015 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 8 0.6914433880726015 TruSeq Adapter, Index 3 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 7 0.6050129645635264 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.6050129645635264 No Hit TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 7 0.6050129645635264 RNA PCR Primer, Index 23 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 6 0.5185825410544511 RNA PCR Primer, Index 23 (96% over 25bp) TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 6 0.5185825410544511 No Hit ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT 5 0.432152117545376 RNA PCR Primer, Index 27 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 5 0.432152117545376 No Hit GTGCTCGCCCACGTCGCTGTCGTAGCGCACGTACTCCTCCCGGTTGTAGA 5 0.432152117545376 No Hit TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG 5 0.432152117545376 TruSeq Adapter, Index 1 (96% over 28bp) ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 5 0.432152117545376 TruSeq Adapter, Index 1 (95% over 21bp) TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 4 0.34572169403630076 RNA PCR Primer, Index 39 (96% over 30bp) TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC 4 0.34572169403630076 No Hit CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT 4 0.34572169403630076 RNA PCR Primer, Index 23 (96% over 28bp) ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT 4 0.34572169403630076 RNA PCR Primer, Index 27 (96% over 25bp) CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA 4 0.34572169403630076 RNA PCR Primer, Index 33 (96% over 29bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.34572169403630076 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 4 0.34572169403630076 No Hit CATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAA 4 0.34572169403630076 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 4 0.34572169403630076 No Hit GTCCTAGACTGTGGTAGAGTCCATGATTGAGGTTCCTGGGACATTCCCAG 4 0.34572169403630076 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 3 0.25929127052722556 TruSeq Adapter, Index 11 (95% over 21bp) CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA 3 0.25929127052722556 No Hit CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA 3 0.25929127052722556 RNA PCR Primer, Index 39 (96% over 30bp) ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 3 0.25929127052722556 RNA PCR Primer, Index 31 (95% over 24bp) GTGTTTGCTATCATAGCAGGATTTGTTCACTGATTCCCATTATTTTCAGG 3 0.25929127052722556 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.25929127052722556 No Hit CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG 3 0.25929127052722556 Illumina PCR Primer Index 1 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 3 0.25929127052722556 RNA PCR Primer, Index 39 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 3 0.25929127052722556 No Hit TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT 3 0.25929127052722556 RNA PCR Primer, Index 31 (95% over 21bp) ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 3 0.25929127052722556 RNA PCR Primer, Index 3 (95% over 23bp) ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 3 0.25929127052722556 RNA PCR Primer, Index 46 (95% over 21bp) TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT 3 0.25929127052722556 No Hit CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 3 0.25929127052722556 RNA PCR Primer, Index 33 (96% over 27bp) TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG 3 0.25929127052722556 RNA PCR Primer, Index 27 (96% over 30bp) ATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGTCTT 2 0.17286084701815038 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 2 0.17286084701815038 RNA PCR Primer, Index 46 (95% over 23bp) GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC 2 0.17286084701815038 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 2 0.17286084701815038 RNA PCR Primer, Index 1 (95% over 23bp) ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 2 0.17286084701815038 RNA PCR Primer, Index 33 (95% over 24bp) CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 2 0.17286084701815038 RNA PCR Primer, Index 31 (96% over 27bp) TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC 2 0.17286084701815038 No Hit TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC 2 0.17286084701815038 No Hit TCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA 2 0.17286084701815038 RNA PCR Primer, Index 38 (96% over 28bp) TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 2 0.17286084701815038 RNA PCR Primer, Index 26 (100% over 29bp) TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT 2 0.17286084701815038 RNA PCR Primer, Index 27 (95% over 22bp) GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 2 0.17286084701815038 No Hit TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT 2 0.17286084701815038 No Hit CCCATGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGC 2 0.17286084701815038 No Hit CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG 2 0.17286084701815038 RNA PCR Primer, Index 26 (96% over 27bp) TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT 2 0.17286084701815038 No Hit CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA 2 0.17286084701815038 RNA PCR Primer, Index 3 (96% over 28bp) CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT 2 0.17286084701815038 RNA PCR Primer, Index 39 (96% over 28bp) CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT 2 0.17286084701815038 TruSeq Adapter, Index 1 (96% over 26bp) GCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACT 2 0.17286084701815038 No Hit CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC 2 0.17286084701815038 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.17286084701815038 0.0 26-27 0.0 0.0 0.0 0.43215211754537597 0.0 28-29 0.0 0.0 0.0 1.2100259291270528 0.0 30-31 0.0 0.0 0.0 2.4200518582541055 0.0 32-33 0.0 0.0 0.0 3.0682800345721697 0.0 34-35 0.0 0.0 0.0 3.9325842696629216 0.0 36-37 0.0 0.0 0.0 5.4019014693172 0.0 38-39 0.0 0.0 0.0 7.433016421780467 0.0 40-41 0.0 0.0 0.0 9.118409680207433 0.0 42-43 0.0 0.0 0.0 10.544511668107173 0.0 44-45 0.0 0.0 0.0 11.106309420916162 0.0 46-47 0.0 0.0 0.0 11.624891961970613 0.0 48-49 0.0 0.0 0.0 12.100259291270527 0.0 50-51 0.0 0.0 0.0 12.618841832324978 0.0 52-53 0.0 0.0 0.0 13.915298184961106 0.0 54-55 0.0 0.0 0.0 14.347450302506482 0.0 56-57 0.0 0.0 0.0 16.335350043215215 0.0 58-59 0.0 0.0 0.0 17.891097666378563 0.0 60-61 0.0 0.0 0.0 18.798617113223855 0.0 62-63 0.0 0.0 0.0 19.706136560069147 0.0 64-65 0.0 0.0 0.0 22.342264477095938 0.0 66-67 0.0 0.0 0.0 24.28694900605013 0.0 68-69 0.0 0.0 0.0 24.80553154710458 0.0 70-71 0.0 0.0 0.0 26.79343128781331 0.0 72-73 0.0 0.0 0.0 29.559204840103718 0.0 74-75 0.0 0.0 0.0 30.89887640449438 0.0 76-77 0.0 0.0 0.0 31.936041486603287 0.0 78-79 0.0 0.0 0.0 32.973206568712186 0.0 80-81 0.0 0.0 0.0 33.7942955920484 0.0 82-83 0.0 0.0 0.0 33.96715643906655 0.0 84-85 0.0 0.0 0.0 34.010371650821085 0.0 86-87 0.0 0.0 0.0 34.1400172860847 0.0 88-89 0.0 0.0 0.0 34.1400172860847 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE