FastQCFastQC Report
Thu 26 May 2016
SRR937292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1159
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT171.4667817083692838RNA PCR Primer, Index 33 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121.0353753235547885No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT121.0353753235547885No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT110.9490940465918896No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT110.9490940465918896RNA PCR Primer, Index 31 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.8628127696289906No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT100.8628127696289906No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT90.7765314926660914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.6902502157031924No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT70.6039689387402933RNA PCR Primer, Index 46 (95% over 21bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG70.6039689387402933RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT60.5176876617773942RNA PCR Primer, Index 31 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT60.5176876617773942RNA PCR Primer, Index 23 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT60.5176876617773942RNA PCR Primer, Index 27 (95% over 23bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG60.5176876617773942RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG60.5176876617773942RNA PCR Primer, Index 31 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT50.4314063848144953TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT50.4314063848144953RNA PCR Primer, Index 23 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT50.4314063848144953RNA PCR Primer, Index 33 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT50.4314063848144953No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC50.4314063848144953No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG50.4314063848144953TruSeq Adapter, Index 1 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.4314063848144953No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG50.4314063848144953RNA PCR Primer, Index 33 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT40.3451251078515962TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT40.3451251078515962No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT40.3451251078515962RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT40.3451251078515962RNA PCR Primer, Index 39 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT40.3451251078515962No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2588438308886971No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT30.2588438308886971No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG30.2588438308886971RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT30.2588438308886971RNA PCR Primer, Index 46 (95% over 23bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA30.2588438308886971RNA PCR Primer, Index 23 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT30.2588438308886971No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT30.2588438308886971RNA PCR Primer, Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT30.2588438308886971No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT30.2588438308886971TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT30.2588438308886971RNA PCR Primer, Index 27 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC30.2588438308886971No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG30.2588438308886971TruSeq Adapter, Index 11 (96% over 28bp)
CATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGAAGTAA30.2588438308886971No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT30.2588438308886971RNA PCR Primer, Index 31 (95% over 21bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA30.2588438308886971RNA PCR Primer, Index 31 (96% over 29bp)
GCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACG30.2588438308886971No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT30.2588438308886971RNA PCR Primer, Index 26 (100% over 22bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT30.2588438308886971RNA PCR Primer, Index 33 (96% over 27bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG30.2588438308886971RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG20.1725625539257981RNA PCR Primer, Index 46 (96% over 28bp)
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG20.1725625539257981RNA PCR Primer, Index 15 (95% over 24bp)
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC20.1725625539257981Illumina PCR Primer Index 1 (95% over 24bp)
TTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT20.1725625539257981RNA PCR Primer, Index 38 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC20.1725625539257981No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT20.1725625539257981No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC20.1725625539257981No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT20.1725625539257981TruSeq Adapter, Index 3 (96% over 26bp)
TATCACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT20.1725625539257981RNA PCR Primer, Index 31 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC20.1725625539257981No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGT20.1725625539257981No Hit
GTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAACGAGA20.1725625539257981No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT20.1725625539257981RNA PCR Primer, Index 3 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT20.1725625539257981No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT20.1725625539257981RNA PCR Primer, Index 26 (100% over 27bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG20.1725625539257981TruSeq Adapter, Index 10 (96% over 28bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG20.1725625539257981RNA PCR Primer, Index 26 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC20.1725625539257981No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT20.1725625539257981TruSeq Adapter, Index 1 (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1725625539257981No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers