FastQCFastQC Report
Thu 26 May 2016
SRR937291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275678
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.2742329819572109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.2147432874585567No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4290.15561633499952846No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3940.14292036361262053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.1382047170974833No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3370.12224406735394192No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3180.11535196860104906No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGC250.00500832157.0255663
ACCGTGC352.7755415E-454.319958
CCGCGCC459.5824804E-442.2488489
ACAGCCG651.3206451E-436.5615045
GTACTAG550.002565974834.5735361
ACACCGT550.002575159334.5483676
AGGATCG600.00393360231.686645
GTGTATA1601.875378E-929.7116361
CCGGGCC650.005818475529.2492059
GTATCAA12350.026.1751081
CTAACCT950.001219686525.0157684
TACATGG6600.024.4806732
GTACATG7550.023.9267221
TACCTGG1801.9314211E-723.760652
ACATGGG6450.023.576463
TAGCACA1252.2402503E-422.814384
TACATGA1252.2430158E-422.8102262
GTGTAAG1503.003472E-522.1846891
GGTATCA9500.022.0178851
CAGTCTA1302.9211416E-421.9369039
Produced by FastQC (version 0.11.5)