##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937291_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 275678 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.991841931528814 33.0 31.0 34.0 31.0 34.0 2 32.13454102249726 33.0 31.0 34.0 31.0 34.0 3 32.17274501411067 34.0 31.0 34.0 30.0 34.0 4 35.75166317225168 37.0 35.0 37.0 35.0 37.0 5 35.74210854692794 37.0 35.0 37.0 35.0 37.0 6 35.788379921502624 37.0 35.0 37.0 35.0 37.0 7 35.74056326583913 37.0 35.0 37.0 35.0 37.0 8 35.706530807681425 37.0 35.0 37.0 35.0 37.0 9 37.37975826870479 39.0 37.0 39.0 35.0 39.0 10-11 37.278672944522235 39.0 37.0 39.0 35.0 39.0 12-13 37.1688364686337 39.0 37.0 39.0 34.0 39.0 14-15 38.38044747858008 40.0 38.0 41.0 34.0 41.0 16-17 38.2955767235688 40.0 38.0 41.0 34.0 41.0 18-19 38.21550504574177 40.0 38.0 41.0 34.0 41.0 20-21 38.069448777196584 40.0 38.0 41.0 34.0 41.0 22-23 38.1304311551883 40.0 38.0 41.0 34.0 41.0 24-25 38.10202482606519 40.0 38.0 41.0 34.0 41.0 26-27 38.02100820522493 40.0 38.0 41.0 34.0 41.0 28-29 37.873635183075905 40.0 38.0 41.0 33.0 41.0 30-31 37.64890923468684 40.0 37.5 41.0 33.0 41.0 32-33 37.42876109083786 40.0 37.0 41.0 32.5 41.0 34-35 37.49678791923911 40.0 37.0 41.0 33.0 41.0 36-37 37.3991432033024 40.0 37.0 41.0 32.5 41.0 38-39 37.26854881419627 40.0 37.0 41.0 32.0 41.0 40-41 37.05709559703712 40.0 37.0 41.0 31.5 41.0 42-43 36.818634421317626 40.0 36.0 41.0 31.0 41.0 44-45 36.60017121424271 39.0 36.0 41.0 30.5 41.0 46-47 36.24319858675702 39.0 36.0 41.0 30.0 41.0 48-49 35.931031856005916 39.0 35.5 40.5 29.0 41.0 50-51 34.999820442690385 38.0 34.0 39.5 27.0 40.5 52-53 35.10463838246069 38.0 35.0 39.5 28.0 40.5 54-55 35.399440651774896 39.0 35.0 40.0 27.5 41.0 56-57 35.149674620390456 38.5 35.0 40.0 26.5 41.0 58-59 34.874342530053184 38.0 34.5 40.0 26.0 41.0 60-61 34.54027162123927 38.0 34.0 40.0 25.5 41.0 62-63 34.170038958495056 38.0 34.0 40.0 24.5 41.0 64-65 33.745115678436434 37.0 33.0 40.0 23.0 41.0 66-67 33.28032704822293 36.5 33.0 39.0 22.0 41.0 68-69 32.77575105739305 36.0 32.0 39.0 20.5 40.0 70-71 32.30941714609073 35.5 32.0 39.0 18.5 40.0 72-73 31.819000790777647 35.0 31.0 38.0 16.5 40.0 74-75 31.26855425532687 35.0 31.0 37.0 10.5 39.0 76-77 30.747591392856883 34.5 30.0 37.0 7.5 39.0 78-79 30.204677195858938 34.0 30.0 36.0 4.5 39.0 80-81 29.64311443060382 34.0 29.0 36.0 2.0 37.5 82-83 29.0995944543997 34.0 29.0 35.0 2.0 37.0 84-85 28.54726528776326 34.0 28.5 35.0 2.0 36.5 86-87 27.984799294829475 33.5 26.5 35.0 2.0 36.0 88-89 27.42694919434993 33.0 26.0 35.0 2.0 36.0 90-91 26.880572624583753 33.0 25.0 35.0 2.0 35.0 92-93 26.219776333258366 32.0 24.5 35.0 2.0 35.0 94-95 25.42676419590972 32.0 21.5 34.0 2.0 35.0 96-97 24.561464824904416 31.5 12.5 34.0 2.0 35.0 98-99 23.325744165294292 31.0 2.0 34.0 2.0 35.0 100-101 20.174589557382163 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.044586788746588724 1101 2 0.14851219227285384 1101 3 0.14221471031066812 1101 4 0.17424393104727898 1101 5 0.22831934423957279 1101 6 0.20679939728969288 1101 7 0.18778707450779564 1101 8 0.20801683068641097 1101 9 0.4256132245666464 1101 10-11 0.2863521551369601 1101 12-13 0.3375444657120852 1101 14-15 0.312760059860139 1101 16-17 -0.04102376857651535 1101 18-19 0.17692472757980227 1101 20-21 0.26067601569516796 1101 22-23 0.36946612496951303 1101 24-25 0.3082743765390319 1101 26-27 0.05825074190456547 1101 28-29 0.11452335363512844 1101 30-31 -0.13853141321598628 1101 32-33 -0.12538474402740007 1101 34-35 0.012954380000891774 1101 36-37 -0.4985314254311959 1101 38-39 -0.4711033763151491 1101 40-41 -0.2422847143918574 1101 42-43 -0.20796046876156993 1101 44-45 -0.41220337218771874 1101 46-47 -0.20413714695109064 1101 48-49 0.15998961510317855 1101 50-51 0.2768936485740525 1101 52-53 0.3591655247545873 1101 54-55 0.33192105437407093 1101 56-57 0.4033899899944373 1101 58-59 0.29774841190157275 1101 60-61 0.20354814838202628 1101 62-63 0.7906290556211744 1101 64-65 0.314862797251763 1101 66-67 0.3278300576943991 1101 68-69 0.43652828974061464 1101 70-71 0.1715209381857612 1101 72-73 0.21715362824912887 1101 74-75 0.33951270268928013 1101 76-77 0.5267219425271676 1101 78-79 0.5702851117586256 1101 80-81 0.6055431004053773 1101 82-83 0.17936227934466586 1101 84-85 0.1874430943259 1101 86-87 0.2828904859563117 1101 88-89 0.2655901356100294 1101 90-91 0.34294512737708516 1101 92-93 0.5905702273276283 1101 94-95 0.6097398396292597 1101 96-97 0.7543541506015785 1101 98-99 0.840198475040566 1101 100-101 1.2372265389531982 1102 1 0.38344218564948207 1102 2 0.5639450800012611 1102 3 0.25373479544553135 1102 4 0.45641772245589607 1102 5 0.37512212428773495 1102 6 0.21199964523400183 1102 7 0.09888916502941214 1102 8 0.2860644337999787 1102 9 0.3332868815806691 1102 10-11 0.4625324962249451 1102 12-13 0.4704771751619461 1102 14-15 0.4370832870857413 1102 16-17 0.5961629411695597 1102 18-19 0.5809532740885643 1102 20-21 0.5708422810065485 1102 22-23 0.4716965266722113 1102 24-25 0.3905622662147863 1102 26-27 0.6957336683056994 1102 28-29 0.6171317058476973 1102 30-31 0.7040458911027443 1102 32-33 0.9590754011187528 1102 34-35 0.8794840921770444 1102 36-37 0.9012266643005091 1102 38-39 0.8140106396695828 1102 40-41 0.8693341096280278 1102 42-43 1.460806227709611 1102 44-45 1.0567926179164502 1102 46-47 1.2865018581997845 1102 48-49 1.587784062686552 1102 50-51 1.4610526401209043 1102 52-53 1.0936786049141105 1102 54-55 1.4578350747471944 1102 56-57 1.4682340099835756 1102 58-59 1.3404749216465532 1102 60-61 1.1929419464829465 1102 62-63 1.0121579724007503 1102 64-65 1.31694728482438 1102 66-67 1.1052314696604597 1102 68-69 0.9365996011575746 1102 70-71 1.5582729225630736 1102 72-73 1.1779762328712025 1102 74-75 1.3140051573928133 1102 76-77 1.5903865813896978 1102 78-79 1.0391604210033307 1102 80-81 1.3358789771792487 1102 82-83 0.9744982921587777 1102 84-85 1.2151311233331903 1102 86-87 1.8615497216076307 1102 88-89 1.5488994241963603 1102 90-91 1.1009854896390827 1102 92-93 1.2577351596640653 1102 94-95 1.3460082777446623 1102 96-97 1.280124999591422 1102 98-99 0.9663977410815185 1102 100-101 0.9016542099771776 1103 1 0.09504983920963994 1103 2 0.15645020021086253 1103 3 0.28056284865880343 1103 4 0.16800692481027113 1103 5 0.1543257702460039 1103 6 0.21483190532219965 1103 7 0.21575910247982222 1103 8 0.10463372730330889 1103 9 0.3084331073865272 1103 10-11 0.27038163916643754 1103 12-13 0.26785064601826747 1103 14-15 -0.11929437219428962 1103 16-17 0.022385794833049033 1103 18-19 0.09971815037322784 1103 20-21 -0.16641716139801588 1103 22-23 0.09272259822598983 1103 24-25 0.28360985187450893 1103 26-27 -0.09663491298109506 1103 28-29 -0.09498335587157669 1103 30-31 0.06322628854171342 1103 32-33 -0.1547270233696878 1103 34-35 0.0969177139642241 1103 36-37 0.08358915668938494 1103 38-39 0.2943001390883708 1103 40-41 0.28134030923316544 1103 42-43 -0.40442616522727093 1103 44-45 -0.06746702745137156 1103 46-47 0.06929887648492894 1103 48-49 0.21626442137798563 1103 50-51 0.060393932074333634 1103 52-53 -0.28329336770431013 1103 54-55 0.31864379109681096 1103 56-57 0.01419135079579803 1103 58-59 0.03484914900231928 1103 60-61 0.04303973787361315 1103 62-63 0.27971429470641596 1103 64-65 -0.11445113206216462 1103 66-67 0.5285482584126058 1103 68-69 0.32142717463949566 1103 70-71 -0.02560625894144053 1103 72-73 -0.0420128809173832 1103 74-75 0.11384647702305273 1103 76-77 0.21742313322835827 1103 78-79 0.18713447860799448 1103 80-81 0.22929097415325117 1103 82-83 0.23568433566672198 1103 84-85 0.5707827276655344 1103 86-87 0.9795451826110089 1103 88-89 0.2844429044627965 1103 90-91 0.09781188224383897 1103 92-93 0.45000133675873855 1103 94-95 0.708256188145608 1103 96-97 0.9934393896868166 1103 98-99 1.399733534575624 1103 100-101 1.6641779659046279 1104 1 8.268979717733771E-4 1104 2 -0.05474904830181515 1104 3 0.08436725320947858 1104 4 0.12782322791015588 1104 5 0.08919686929620241 1104 6 0.24256909350715006 1104 7 0.1401406910405143 1104 8 -0.09464087868771287 1104 9 -0.04995850100507937 1104 10-11 0.11156470982712818 1104 12-13 0.010896778989767597 1104 14-15 0.327840582403347 1104 16-17 0.31099084649779485 1104 18-19 0.3600902409691997 1104 20-21 0.38702193681720587 1104 22-23 -0.006914704769521052 1104 24-25 -0.018955120466586095 1104 26-27 0.09265900205910071 1104 28-29 0.20733373002759237 1104 30-31 -0.07455943319923364 1104 32-33 -0.12190163979802549 1104 34-35 -0.2570249851277282 1104 36-37 -0.20544643458501355 1104 38-39 -0.34488157472021186 1104 40-41 -0.022294488804618595 1104 42-43 -0.2787085992111926 1104 44-45 0.17325680095901674 1104 46-47 -0.2335922622270985 1104 48-49 -0.006272370188654008 1104 50-51 -0.21339011969479316 1104 52-53 -0.10066629328618859 1104 54-55 0.086335055056729 1104 56-57 0.5194882926599007 1104 58-59 0.20992221026940427 1104 60-61 0.32790039959994743 1104 62-63 -0.13116400609725787 1104 64-65 -0.004376505196496794 1104 66-67 0.26117011416878455 1104 68-69 0.2034201816325023 1104 70-71 0.5379179034633275 1104 72-73 0.4717382932815539 1104 74-75 0.4808227842978674 1104 76-77 0.27289750959825554 1104 78-79 0.34041962629612854 1104 80-81 0.5189658515146967 1104 82-83 0.7719670827703666 1104 84-85 0.26333830641513956 1104 86-87 0.30529406059869046 1104 88-89 0.2921012908525249 1104 90-91 0.21718877400879322 1104 92-93 -0.09739716026662038 1104 94-95 0.19745355793999053 1104 96-97 -0.23747335428562977 1104 98-99 0.1528516850818349 1104 100-101 0.2173169910585777 1105 1 -0.08394660294469602 1105 2 -0.20341362017927267 1105 3 0.17830545692773114 1105 4 0.40957237690204096 1105 5 0.20297010836402052 1105 6 0.19366885784336318 1105 7 0.44251042133640794 1105 8 0.35816090188311733 1105 9 0.16933974460895485 1105 10-11 0.4098430549436429 1105 12-13 0.4444854147582973 1105 14-15 0.49967875993673516 1105 16-17 0.588681341391748 1105 18-19 0.5154646845408095 1105 20-21 0.330331703771904 1105 22-23 0.38395986972641793 1105 24-25 0.1956298494208255 1105 26-27 0.3335270303387503 1105 28-29 0.12753535348923606 1105 30-31 0.1490593151642159 1105 32-33 -0.09375290976399242 1105 34-35 0.10576632149704324 1105 36-37 0.1947564981198866 1105 38-39 0.1801577025775103 1105 40-41 0.19759291627524078 1105 42-43 0.4014105419252303 1105 44-45 0.12766240452015865 1105 46-47 -0.26455586526454766 1105 48-49 0.04128589113629744 1105 50-51 -0.4115113556204264 1105 52-53 -0.052846183309746664 1105 54-55 0.324977369798809 1105 56-57 0.07860062573138293 1105 58-59 -0.19679420764104094 1105 60-61 -0.18725409110442826 1105 62-63 -0.49896316818157516 1105 64-65 -0.37980200925515106 1105 66-67 -0.6359560968812232 1105 68-69 -0.5400008709990765 1105 70-71 0.21718603648243473 1105 72-73 -0.5333022932594247 1105 74-75 -0.9903738640393946 1105 76-77 -0.6779731371679105 1105 78-79 -0.5285905858012789 1105 80-81 -0.40820749229258624 1105 82-83 0.13187805422886356 1105 84-85 -0.03153600623214814 1105 86-87 0.07175779456046172 1105 88-89 -0.19654102388955508 1105 90-91 -0.19492827233841936 1105 92-93 -0.1513052513899531 1105 94-95 0.4765514893356446 1105 96-97 0.3006256245046295 1105 98-99 0.6727521825942695 1105 100-101 -0.3799270518846001 1106 1 -0.19865645449665337 1106 2 -0.9764169326562673 1106 3 -0.43272386462790635 1106 4 0.04213279893614441 1106 5 -0.07294695702672982 1106 6 -0.14530795481766035 1106 7 -0.04997516325444451 1106 8 -0.27298865031906416 1106 9 -0.02023822128479935 1106 10-11 -0.6666812978964956 1106 12-13 -0.873757745590126 1106 14-15 -1.5458677987677234 1106 16-17 -0.6244673520200976 1106 18-19 -0.40377835312327903 1106 20-21 -0.007326252307109371 1106 22-23 -0.03489977939639033 1106 24-25 -0.08701951875485747 1106 26-27 -0.37985169619786774 1106 28-29 -0.25232601321424397 1106 30-31 -0.20600448068905308 1106 32-33 0.042825424182765914 1106 34-35 -0.06916620211969615 1106 36-37 -0.05976748666725484 1106 38-39 -0.16898657419834962 1106 40-41 -0.6207575928647344 1106 42-43 -0.5477828085839107 1106 44-45 -0.539494999479345 1106 46-47 0.08852964571570254 1106 48-49 -0.030239075125507497 1106 50-51 -0.4640815924011932 1106 52-53 -0.21860805301898978 1106 54-55 -0.46981774736473625 1106 56-57 -0.8057387191342755 1106 58-59 -1.2973186831655212 1106 60-61 -1.4202469754131002 1106 62-63 -1.312943047950931 1106 64-65 -0.9606049782160113 1106 66-67 -0.5938293639650212 1106 68-69 -0.32192946871714767 1106 70-71 -0.4102216435568202 1106 72-73 -0.3199849088894098 1106 74-75 -1.1518877887112104 1106 76-77 -0.6881712723660449 1106 78-79 -0.10832006684654871 1106 80-81 0.09117908604136105 1106 82-83 -0.2503296503472612 1106 84-85 -0.7701263632435591 1106 86-87 -0.14632894326311785 1106 88-89 -0.3012214312015402 1106 90-91 -0.2343559499239909 1106 92-93 0.28391742067482184 1106 94-95 0.5666477965372145 1106 96-97 -0.06250466625724016 1106 98-99 -0.5932734584313692 1106 100-101 -0.6050528033261422 1107 1 0.04653585319565323 1107 2 0.023889011399674587 1107 3 0.09891949201544747 1107 4 -0.06564692134357841 1107 5 -0.2530605933903658 1107 6 0.0588004367907331 1107 7 -0.02086677164604822 1107 8 0.12438897205855426 1107 9 0.20004149899492063 1107 10-11 -0.0042468573370584295 1107 12-13 0.1675665551091754 1107 14-15 0.1608629704630502 1107 16-17 0.3031550256022726 1107 18-19 -0.25991908738901515 1107 20-21 -0.4679687348245949 1107 22-23 -0.12864977939639033 1107 24-25 0.12041055117521182 1107 26-27 -0.24591375913493607 1107 28-29 0.04968447629624961 1107 30-31 0.24768870112912822 1107 32-33 0.03952559900793773 1107 34-35 0.5750645671110775 1107 36-37 0.36027184899707976 1107 38-39 0.0561445446827733 1107 40-41 0.2430973022401588 1107 42-43 0.19461229631119892 1107 44-45 0.19876986066051217 1107 46-47 0.1429002750863262 1107 48-49 0.2882705402591057 1107 50-51 0.5502759250813298 1107 52-53 0.4923840798481436 1107 54-55 0.7135738610268803 1107 56-57 0.8512979941524321 1107 58-59 0.9500807923589534 1107 60-61 0.7174059966148789 1107 62-63 0.7215412177833329 1107 64-65 0.3199238679378382 1107 66-67 0.46541451715385307 1107 68-69 0.8721701816325051 1107 70-71 0.7692145452543606 1107 72-73 1.2223446365651398 1107 74-75 0.9322686798202575 1107 76-77 0.817860196165423 1107 78-79 0.5531528352513497 1107 80-81 0.6882289112161892 1107 82-83 1.039224545456932 1107 84-85 0.5128718885046943 1107 86-87 0.6677007770166021 1107 88-89 0.46798748488237507 1107 90-91 0.7318809381878957 1107 92-93 0.7036289591363634 1107 94-95 1.040970348984768 1107 96-97 0.25473746660989605 1107 98-99 0.4831688492609345 1107 100-101 0.07473303583469715 1108 1 0.3976956470100852 1108 2 0.26660808356462695 1108 3 0.253816399231944 1108 4 -0.03897166361161908 1108 5 0.14635950970242106 1108 6 0.18444476668764054 1108 7 0.3107053933023991 1108 8 0.21536835350185157 1108 9 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-0.5866683116859264 2316 84-85 -0.18266382578102025 2316 86-87 -0.37247779441196727 2316 88-89 0.07914819916809179 2316 90-91 0.4037559381878957 2316 92-93 0.5045218162792189 2316 94-95 1.2405239204133416 2316 96-97 1.995362466609894 2316 98-99 2.6679902778323665 2316 100-101 2.3662508929775576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1255.0 3 199.0 4 191.0 5 212.0 6 239.0 7 247.0 8 296.0 9 370.0 10 471.0 11 598.0 12 819.0 13 990.0 14 1164.0 15 1508.0 16 1978.0 17 2493.0 18 2279.0 19 1676.0 20 1726.0 21 1928.0 22 2123.0 23 2261.0 24 2688.0 25 2946.0 26 3349.0 27 3898.0 28 4621.0 29 5608.0 30 6819.0 31 8620.0 32 11325.0 33 14838.0 34 20155.0 35 29047.0 36 41601.0 37 53712.0 38 37511.0 39 3917.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.72549668753706 18.91760134754121 15.091079088008557 23.26582287691317 2 14.68412707648445 19.604413347685686 41.33295028074361 24.37850929508626 3 19.003149251234607 23.866669575905682 30.41100274195777 26.719178430901934 4 12.575551498665346 16.07619518652401 38.22596716828255 33.12228614652808 5 13.526801949232672 36.27827478362063 35.690959342497635 14.503963924649065 6 31.574087202475088 35.96075052442912 18.129069551844864 14.336092721250923 7 27.620581466791243 32.07483376535557 22.152379636665053 18.15220513118813 8 25.54297639043402 34.50508620000146 20.87886918395393 19.073068225610598 9 25.02627053010112 16.824774655210657 21.389847394581544 36.75910742010669 10-11 24.140940329442564 26.106323406421584 28.609868732046106 21.142867532089742 12-13 24.918782030421973 23.473311238894453 29.041400712282044 22.566506018401526 14-15 22.14353103282792 25.167102639285527 26.981100177964084 25.70826614992247 16-17 21.402227437065903 28.21777768891135 27.97129452738308 22.40870034663967 18-19 21.397310864499993 27.12471874579709 30.046854496614046 21.43111589308887 20-21 21.943496745509965 26.158429410920036 30.019830486601247 21.878243356968753 22-23 21.65223523595571 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29.22481831963384 22.323872745582047 48-49 21.596182685753238 26.876618950238583 29.02485798682118 22.502340377187004 50-51 21.716484930899203 27.054208040489687 29.13214050677555 22.09716652183556 52-53 21.77680488563164 26.93588063001231 28.83071737260941 22.456597111746643 54-55 21.525241636566243 26.955315640889072 28.857323083131703 22.662119639412985 56-57 21.623129615190987 26.84848451803912 28.989078933737535 22.539306933032364 58-59 21.799985825166146 26.62161007939724 29.032967392430276 22.545436703006335 60-61 21.51344317591169 26.49688682434274 29.555722754715198 22.43394724503037 62-63 21.28915940743595 26.358958887279428 29.85774755142952 22.494134153855104 64-65 21.307250833251555 26.46068906359608 29.528726733472176 22.703333369680184 66-67 21.23249829162971 26.430487503453087 29.220946800622283 23.116067404294917 68-69 21.26429156890576 26.205798592242978 29.5688948580339 22.961014980817357 70-71 21.2266225622944 26.119118154886316 29.478380981806946 23.17587830101234 72-73 21.084772008134973 26.039180904988395 29.386223980720334 23.489823106156297 74-75 20.97862317391889 26.573637549934393 29.30399002584411 23.143749250302605 76-77 21.152112430294526 26.195080811091803 29.310288418894455 23.342518339719216 78-79 21.079035477081128 25.880928751376874 29.382650075069343 23.657385696472648 80-81 21.17577564114215 25.719115214743997 29.17955615332885 23.925552990785 82-83 21.201183517985402 25.362397859780746 29.76042484515397 23.675993777079878 84-85 20.731414780946288 25.426694967616253 29.586062266935137 24.255827984502325 86-87 21.173962664650638 25.274962926347012 29.741065976563636 23.81000843243872 88-89 20.60703543160401 24.728151722508475 29.926119789536816 24.738693056350698 90-91 20.39045631992556 24.876778353700647 30.334879359102047 24.397885967271748 92-93 20.20618032053915 25.323065514596564 30.249292073035527 24.221462091828762 94-95 19.83341753931935 25.478611073753537 30.04717958410724 24.64079180281987 96-97 19.75383138976939 25.9605737633408 29.93954837583971 24.346046471050105 98-99 20.1896844106533 26.070337364157353 29.096272654073147 24.643705571116204 100-101 20.580334912055598 25.37393181809921 29.094894172297604 24.95083909754759 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 384.0 1 220.5 2 44.5 3 33.0 4 31.5 5 30.0 6 41.0 7 73.0 8 121.0 9 166.0 10 177.0 11 193.0 12 216.5 13 231.0 14 243.0 15 258.0 16 278.0 17 294.0 18 324.5 19 354.5 20 424.5 21 519.5 22 616.5 23 728.0 24 866.0 25 1115.0 26 1448.0 27 1842.5 28 2265.0 29 2713.0 30 3240.0 31 3888.0 32 4587.0 33 5263.0 34 5793.0 35 6309.0 36 7012.0 37 7583.0 38 8115.0 39 8637.5 40 8928.5 41 9368.5 42 9815.5 43 10054.5 44 10340.5 45 10531.5 46 10738.0 47 11476.0 48 12171.0 49 11765.5 50 10743.0 51 9969.5 52 9496.5 53 9200.5 54 8798.0 55 8058.5 56 7069.5 57 6086.5 58 5454.0 59 4647.0 60 3650.5 61 2922.5 62 2273.5 63 1648.0 64 1131.5 65 805.0 66 560.0 67 395.5 68 302.0 69 242.0 70 189.0 71 114.5 72 69.0 73 47.5 74 35.5 75 28.0 76 19.5 77 14.0 78 10.0 79 5.5 80 2.0 81 2.0 82 1.5 83 1.5 84 1.5 85 1.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29273282597813394 2 0.25174297550040264 3 0.25101749142115076 4 0.2539194277381583 5 0.2531939436589064 6 0.2238118384492052 7 0.22308635436995333 8 0.22490006456808306 9 0.23759603595499096 10-11 0.2423116824701282 12-13 0.2345127286181705 14-15 0.22580691966714792 16-17 0.22072853111238475 18-19 0.20712570462641197 20-21 0.21637562663687346 22-23 0.2118413511415492 24-25 0.21438054541893076 26-27 0.21220409318117514 28-29 0.2203657890727588 30-31 0.23433135759835752 32-33 0.22363046742939224 34-35 0.22635103272658683 36-37 0.2152874005179956 38-39 0.20803255972547682 40-41 0.2064002205471601 42-43 0.21492465847836967 44-45 0.2138364323594919 46-47 0.22018441805294584 48-49 0.2232677253897663 50-51 0.23396861555873158 52-53 0.24213031145031522 54-55 0.22725788782565165 56-57 0.20585610748772115 58-59 0.1967875564970727 60-61 0.2020473160716488 62-63 0.2062188495273471 64-65 0.19987086383389316 66-67 0.2038610262697785 68-69 0.20295417117071365 70-71 0.20785118870566385 72-73 0.20658159156697306 74-75 0.20567473646790824 76-77 0.2147432874585567 78-79 0.2172824817359383 80-81 0.21147860910192326 82-83 0.2053119944282823 84-85 0.19588070139800784 86-87 0.19950812179426722 88-89 0.206944333606599 90-91 0.20458651034903041 92-93 0.21002764094341947 94-95 0.20277280015090068 96-97 0.21111586706229732 98-99 0.2138364323594919 100-101 0.20349828423015257 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 275678.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.18313533444348 #Duplication Level Percentage of deduplicated Percentage of total 1 88.31107940267914 68.16125993067767 2 7.34522366541993 11.338547844597267 3 1.9231870064737508 4.453128089823203 4 0.8520762489209568 2.6306366574292466 5 0.4208238507583415 1.6240252112521356 6 0.2821320420086812 1.3065501348323358 7 0.19109773299720645 1.0324665531620112 8 0.1442874785640875 0.8909247988062063 9 0.09621938094495891 0.6683862151144021 >10 0.40843227217829 5.243928464356173 >50 0.0169606127030836 0.9068968679025655 >100 0.00753805009025921 1.253074020365753 >500 9.422562612824012E-4 0.49017521168108974 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 756 0.2742329819572109 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 592 0.2147432874585567 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 429 0.15561633499952846 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 394 0.14292036361262053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 381 0.1382047170974833 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 337 0.12224406735394192 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 318 0.11535196860104906 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.8137101981297022E-4 14-15 0.0 0.0 0.0 0.0 3.6274203962594043E-4 16-17 0.0 0.0 0.0 3.6274203962594043E-4 3.6274203962594043E-4 18-19 0.0 0.0 0.0 3.6274203962594043E-4 3.6274203962594043E-4 20-21 0.0 0.0 0.0 9.068550990648511E-4 3.6274203962594043E-4 22-23 0.0 0.0 0.0 0.001813710198129702 3.6274203962594043E-4 24-25 0.0 0.0 0.0 0.0030833073368204938 3.6274203962594043E-4 26-27 0.0 0.0 0.0 0.0032646783566334637 3.6274203962594043E-4 28-29 0.0 0.0 0.0 0.0061666146736409876 3.6274203962594043E-4 30-31 0.0 0.0 0.0 0.015053794644476527 3.6274203962594043E-4 32-33 0.0 0.0 0.0 0.022671377476621277 3.6274203962594043E-4 34-35 0.0 0.0 0.0 0.03391638070502542 3.6274203962594043E-4 36-37 0.0 0.0 0.0 0.04915154636931493 3.6274203962594043E-4 38-39 0.0 0.0 0.0 0.07327389200443996 3.6274203962594043E-4 40-41 0.0 0.0 0.0 0.10446970741227084 3.6274203962594043E-4 42-43 0.0 0.0 0.0 0.14818012318719664 3.6274203962594043E-4 44-45 0.0 0.0 0.0 0.19225328100174843 3.6274203962594043E-4 46-47 0.0 0.0 0.0 0.2803995966308519 3.6274203962594043E-4 48-49 0.0 0.0 0.0 0.37670760815153914 3.6274203962594043E-4 50-51 0.0 0.0 0.0 0.4213248790255298 3.6274203962594043E-4 52-53 0.0 0.0 0.0 0.5067506293574388 3.6274203962594043E-4 54-55 0.0 0.0 0.0 0.6607346251786506 3.6274203962594043E-4 56-57 0.0 0.0 0.0 0.9355117201953003 3.6274203962594043E-4 58-59 0.0 0.0 0.0 1.2781215766220009 3.6274203962594043E-4 60-61 0.0 0.0 0.0 1.6693388663585778 3.6274203962594043E-4 62-63 0.0 0.0 0.0 2.087036324987848 3.6274203962594043E-4 64-65 0.0 0.0 0.0 2.5535225879468078 3.6274203962594043E-4 66-67 0.0 0.0 0.0 3.127924607694484 3.6274203962594043E-4 68-69 0.0 0.0 0.0 3.7436792199595184 3.6274203962594043E-4 70-71 0.0 0.0 0.0 4.175160876094574 3.6274203962594043E-4 72-73 0.0 0.0 0.0 4.773866612497189 3.6274203962594043E-4 74-75 0.0 0.0 0.0 5.448385435181625 3.6274203962594043E-4 76-77 0.0 0.0 0.0 6.215766220010302 3.6274203962594043E-4 78-79 0.0 0.0 0.0 7.037195568743243 3.6274203962594043E-4 80-81 0.0 0.0 0.0 8.280675280580969 3.6274203962594043E-4 82-83 0.0 0.0 0.0 9.536306850746161 3.6274203962594043E-4 84-85 0.0 0.0 0.0 11.264409927524142 3.6274203962594043E-4 86-87 0.0 0.0 0.0 12.968753400706621 3.6274203962594043E-4 88-89 0.0 0.0 0.0 14.903982182111013 3.6274203962594043E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACCGC 25 0.005008321 57.025566 3 ACCGTGC 35 2.7755415E-4 54.31995 8 CCGCGCC 45 9.5824804E-4 42.248848 9 ACAGCCG 65 1.3206451E-4 36.561504 5 GTACTAG 55 0.0025659748 34.573536 1 ACACCGT 55 0.0025751593 34.548367 6 AGGATCG 60 0.003933602 31.68664 5 GTGTATA 160 1.875378E-9 29.711636 1 CCGGGCC 65 0.0058184755 29.249205 9 GTATCAA 1235 0.0 26.175108 1 CTAACCT 95 0.0012196865 25.015768 4 TACATGG 660 0.0 24.480673 2 GTACATG 755 0.0 23.926722 1 TACCTGG 180 1.9314211E-7 23.76065 2 ACATGGG 645 0.0 23.57646 3 TAGCACA 125 2.2402503E-4 22.81438 4 TACATGA 125 2.2430158E-4 22.810226 2 GTGTAAG 150 3.003472E-5 22.184689 1 GGTATCA 950 0.0 22.017885 1 CAGTCTA 130 2.9211416E-4 21.936903 9 >>END_MODULE