FastQCFastQC Report
Thu 26 May 2016
SRR937291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275678
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8450.3065170234839197No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8180.29672298841401923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.2963602463743933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.16903779046568823No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC4410.15996923947503974No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC651.3257304E-436.5376933
GGTAAGG1353.0559022E-1035.184453
GTAAGGT1255.6206773E-934.1992844
ATATAAC702.0495521E-433.927863
GTGTAGG851.6219008E-533.5469671
ACTGACC1001.2946603E-633.2493028
GCGTAGG600.003935690531.6832391
CCCTATA600.00394619531.6660022
TAAGGTG1703.616151E-927.9405925
TAATCGG350.00838870927.14228614-15
TACCTGG2155.638867E-1126.5110722
CCTATAG908.9313276E-426.3883343
AATCGGA458.9427154E-426.38833416-17
GTAGATC1109.452377E-525.9085483
GAGTACT9250.025.67513512-13
GTATGTA1701.1107295E-725.1602231
GGTATCA15650.024.9012071
AGGTAAG1852.5276313E-723.1076242
GTACTTT10150.022.93055214-15
GGTGTGC2503.783498E-1022.7995228
Produced by FastQC (version 0.11.5)