##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937291_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 275678 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50055136790023 34.0 31.0 34.0 31.0 34.0 2 32.719970400249565 34.0 31.0 34.0 31.0 34.0 3 32.78580445302128 34.0 31.0 34.0 31.0 34.0 4 36.32045720006674 37.0 37.0 37.0 35.0 37.0 5 36.273395047845675 37.0 37.0 37.0 35.0 37.0 6 36.31732310884438 37.0 37.0 37.0 35.0 37.0 7 36.272698583129596 37.0 37.0 37.0 35.0 37.0 8 36.24242775992281 37.0 37.0 37.0 35.0 37.0 9 38.00093224704184 39.0 38.0 39.0 35.0 39.0 10-11 37.93375060759291 39.0 38.0 39.0 35.0 39.0 12-13 37.85656635640131 39.0 38.0 39.0 35.0 39.0 14-15 39.17764021793542 40.0 38.0 41.0 36.0 41.0 16-17 39.13617154796538 40.0 38.0 41.0 36.0 41.0 18-19 39.096395069610196 40.0 38.5 41.0 36.0 41.0 20-21 38.97620956333113 40.0 38.0 41.0 35.5 41.0 22-23 38.92316760858683 40.0 38.0 41.0 35.0 41.0 24-25 38.950253556685695 40.0 38.0 41.0 35.0 41.0 26-27 38.81869790117456 40.0 38.0 41.0 35.0 41.0 28-29 38.68453050297811 40.0 38.0 41.0 35.0 41.0 30-31 38.78065714347899 40.0 38.0 41.0 35.0 41.0 32-33 38.716165236253886 40.0 38.0 41.0 35.0 41.0 34-35 38.599416710800284 40.0 38.0 41.0 35.0 41.0 36-37 38.46363329681731 40.0 38.0 41.0 35.0 41.0 38-39 38.34373254303934 40.0 38.0 41.0 34.0 41.0 40-41 38.180812397071946 40.0 38.0 41.0 34.0 41.0 42-43 38.007766307068394 40.0 38.0 41.0 33.5 41.0 44-45 37.71841786431997 40.0 37.5 41.0 33.0 41.0 46-47 37.414122635828754 40.0 37.0 41.0 33.0 41.0 48-49 37.10361726361915 40.0 37.0 41.0 32.0 41.0 50-51 36.87113226300249 40.0 37.0 41.0 31.5 41.0 52-53 36.6335416681781 39.5 36.0 41.0 31.0 41.0 54-55 36.42732644607114 39.0 36.0 41.0 31.0 41.0 56-57 36.11449408367733 39.0 35.0 40.0 30.0 41.0 58-59 35.84611394452949 39.0 35.0 40.0 29.5 41.0 60-61 35.4698379994051 38.0 35.0 40.0 29.0 41.0 62-63 35.12433890263278 38.0 34.5 40.0 28.0 41.0 64-65 34.72067049238605 37.0 34.0 40.0 28.0 41.0 66-67 34.28468358011884 37.0 34.0 39.0 26.5 41.0 68-69 33.82499147556207 36.0 33.5 39.0 26.0 40.0 70-71 33.37611815233714 35.5 33.0 38.5 26.0 40.0 72-73 32.8893527956529 35.0 32.5 38.0 25.0 40.0 74-75 32.43993535936854 35.0 32.0 37.0 24.5 39.0 76-77 31.13807957109381 34.0 30.5 36.0 22.5 38.5 78-79 31.77274936701514 34.5 32.0 36.0 24.0 39.0 80-81 31.589735125762665 35.0 32.0 36.0 23.0 37.5 82-83 31.219917802653818 34.0 31.5 35.5 22.0 37.0 84-85 30.823565899346338 34.0 31.0 35.0 20.0 37.0 86-87 30.505094711946548 34.0 31.0 35.0 18.5 36.0 88-89 30.101219538737222 34.0 30.5 35.0 16.5 36.0 90-91 29.62446767605685 34.0 30.0 35.0 8.5 35.0 92-93 29.17167311138357 34.0 29.0 35.0 4.5 35.0 94-95 28.74188364686337 34.0 29.0 35.0 2.0 35.0 96-97 28.12942454602834 33.5 29.0 35.0 2.0 35.0 98-99 27.24586292703807 33.0 27.0 35.0 2.0 35.0 100-101 24.29523937347195 31.0 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14335681783774845 1101 2 0.22079148621141087 1101 3 0.16730180215832036 1101 4 0.04616581884621951 1101 5 0.06873683215010828 1101 6 -0.08823879168009796 1101 7 0.08893509049548243 1101 8 -0.10577293192338288 1101 9 0.1723224406863224 1101 10-11 0.02168071110686043 1101 12-13 0.13598907755207534 1101 14-15 0.21427506688208808 1101 16-17 0.1942257829051499 1101 18-19 0.21481572908800217 1101 20-21 0.2654408822535075 1101 22-23 0.23555496910080365 1101 24-25 0.08329284537676784 1101 26-27 0.0634110209757921 1101 28-29 -0.09359357392015966 1101 30-31 0.08642313622168984 1101 32-33 -0.005720880779094273 1101 34-35 0.19347971019679022 1101 36-37 0.2960037007291305 1101 38-39 0.19114237797429467 1101 40-41 0.5443253894396562 1101 42-43 0.48798135096270556 1101 44-45 0.6063335576304354 1101 46-47 0.731518474028853 1101 48-49 0.9099374210191371 1101 50-51 1.0077387497396657 1101 52-53 0.9519903117757451 1101 54-55 0.9014253044616538 1101 56-57 0.9271389694162622 1101 58-59 0.9943468609207784 1101 60-61 1.0493113706752553 1101 62-63 0.9200138161838183 1101 64-65 0.8147714981998462 1101 66-67 0.7660470500655663 1101 68-69 1.0289204797358877 1101 70-71 0.7040807815606129 1101 72-73 0.5427566116638758 1101 74-75 0.9982315816345952 1101 76-77 0.661910083936192 1101 78-79 0.7022015028858313 1101 80-81 0.7837839055220819 1101 82-83 0.629353246928126 1101 84-85 0.8341350829625114 1101 86-87 0.6904892956896767 1101 88-89 0.7271127753097062 1101 90-91 0.6407605432305701 1101 92-93 0.8803514289487069 1101 94-95 1.1057880972227103 1101 96-97 1.154368867086415 1101 98-99 1.3609666978913957 1101 100-101 2.4229966032960384 1102 1 0.30994028785129757 1102 2 0.24599184386558903 1102 3 0.34433513750300193 1102 4 0.2041315783950921 1102 5 0.17329582589115944 1102 6 0.2602710739801637 1102 7 0.20060876945148465 1102 8 0.27072940763964226 1102 9 0.40394740845904664 1102 10-11 0.2825845010343997 1102 12-13 0.303181437356578 1102 14-15 0.1572451325434443 1102 16-17 0.451624342414533 1102 18-19 0.42939726820780066 1102 20-21 0.48194782963117433 1102 22-23 0.6251292399415647 1102 24-25 0.30557601752801844 1102 26-27 0.34890902151337144 1102 28-29 0.37058889553935614 1102 30-31 0.4014660053542727 1102 32-33 0.5915012652557223 1102 34-35 0.4605629086783978 1102 36-37 0.5653277233513023 1102 38-39 0.6070415326597285 1102 40-41 0.6333138095626438 1102 42-43 0.6806099446467115 1102 44-45 0.6136813763239033 1102 46-47 0.6540166775296967 1102 48-49 0.5159034438717072 1102 50-51 0.7070294754156166 1102 52-53 0.7226253673712293 1102 54-55 0.622188994882336 1102 56-57 0.48057864818575524 1102 58-59 0.6639007257253056 1102 60-61 0.6886237091960368 1102 62-63 0.7948732504100136 1102 64-65 0.49637973521704737 1102 66-67 0.4923922302366037 1102 68-69 0.5905746851895657 1102 70-71 0.517979925275057 1102 72-73 0.5846164134181393 1102 74-75 0.7134329815325664 1102 76-77 0.4839826131327314 1102 78-79 0.5438901572393924 1102 80-81 0.7995409859229632 1102 82-83 0.6264020513647921 1102 84-85 0.6036867535695905 1102 86-87 0.8515379026485732 1102 88-89 0.6778640136598924 1102 90-91 0.8293874693245122 1102 92-93 1.0272172921734075 1102 94-95 1.408623000321466 1102 96-97 1.3949571314213784 1102 98-99 1.7320932537145204 1102 100-101 2.4311507895504896 1103 1 0.2321869066678346 1103 2 0.3142520796720021 1103 3 0.22532486018137377 1103 4 0.07007434275474367 1103 5 0.19716246057573272 1103 6 0.1547209512796499 1103 7 0.0014280029883906309 1103 8 0.19010686395640874 1103 9 0.14775241611629752 1103 10-11 -0.018954329528177993 1103 12-13 0.29474923631223504 1103 14-15 0.47759958020660065 1103 16-17 0.3591284478078194 1103 18-19 0.38123039550266924 1103 20-21 0.3433562101688352 1103 22-23 0.39096037450620713 1103 24-25 0.20165421373813075 1103 26-27 0.449491157055931 1103 28-29 0.2669715366449523 1103 30-31 0.11779716759571812 1103 32-33 0.25935188429367173 1103 34-35 0.5051410218581012 1103 36-37 0.48854764327307265 1103 38-39 0.1495623363942471 1103 40-41 0.1361322312464992 1103 42-43 0.28296339594475484 1103 44-45 0.327180278477158 1103 46-47 0.5826335751439586 1103 48-49 0.5783838394655589 1103 50-51 0.6609706529715709 1103 52-53 0.37379148357692316 1103 54-55 0.37227202530837644 1103 56-57 0.7132852555625462 1103 58-59 0.30558292215683736 1103 60-61 0.5563516277155074 1103 62-63 0.5404541866241956 1103 64-65 0.44948138290972395 1103 66-67 0.26661405063256893 1103 68-69 0.2149911658065804 1103 70-71 0.3871747146545417 1103 72-73 0.3123181312253962 1103 74-75 0.2861261195291327 1103 76-77 0.39820757023368003 1103 78-79 0.13227143295576127 1103 80-81 0.4957003674385412 1103 82-83 0.5369321545070349 1103 84-85 0.8056905545179802 1103 86-87 0.49185009705047733 1103 88-89 0.3620116602085943 1103 90-91 0.8309419834120106 1103 92-93 0.7614230600203378 1103 94-95 0.9643687058033201 1103 96-97 1.5064762191937646 1103 98-99 1.5408003777250734 1103 100-101 1.622439052738489 1104 1 0.13266702356586535 1104 2 0.17032138350249681 1104 3 0.18143591330735376 1104 4 -0.08951233922000057 1104 5 -0.05521203060472857 1104 6 0.056740573448522014 1104 7 0.10133928573549866 1104 8 -0.14250455447590582 1104 9 0.17668989654631417 1104 10-11 0.12299327510599056 1104 12-13 0.005609271799137616 1104 14-15 0.045363905433610796 1104 16-17 0.12353195474864265 1104 18-19 0.03090161980075834 1104 20-21 0.14395113762943623 1104 22-23 -0.05120380459827345 1104 24-25 0.11922544847354999 1104 26-27 0.08354553551329502 1104 28-29 0.029835538314927135 1104 30-31 0.058930660968023574 1104 32-33 0.29867450197449585 1104 34-35 0.321326701849749 1104 36-37 0.4498382183696208 1104 38-39 0.4276909242243292 1104 40-41 0.334063031789249 1104 42-43 0.16768315379884058 1104 44-45 0.26856165829137524 1104 46-47 0.23371383816451896 1104 48-49 0.30534344702218164 1104 50-51 0.23647164451734426 1104 52-53 0.4245560175504153 1104 54-55 0.17760288847506445 1104 56-57 0.31026104097117013 1104 58-59 0.31722053827207475 1104 60-61 0.6619382053170142 1104 62-63 0.7944203696948549 1104 64-65 0.30093217092768043 1104 66-67 0.21745425520411743 1104 68-69 0.5839636112715567 1104 70-71 0.4802234569570203 1104 72-73 0.5291926745924727 1104 74-75 0.14527443390132433 1104 76-77 0.4331073719245282 1104 78-79 0.33279851781120584 1104 80-81 0.5385586285803861 1104 82-83 0.6702428736386494 1104 84-85 0.18404354481127427 1104 86-87 0.6604911574825856 1104 88-89 0.5582985822417825 1104 90-91 0.7249640061653757 1104 92-93 0.5637923325388634 1104 94-95 0.6059510041020353 1104 96-97 0.7160446358517341 1104 98-99 1.1975167956355222 1104 100-101 2.0417324459124764 1105 1 0.21246540010422876 1105 2 0.09464712322493796 1105 3 0.046911168609788945 1105 4 0.15374505800440375 1105 5 0.1871943585023672 1105 6 0.06237031945533289 1105 7 0.12497112653151987 1105 8 0.10723359687261791 1105 9 0.21982946187755914 1105 10-11 0.022574317263625687 1105 12-13 0.02662262608036059 1105 14-15 0.28737752156349217 1105 16-17 0.16441307546087103 1105 18-19 0.20323076355566627 1105 20-21 0.003796646925060543 1105 22-23 0.08613581310270035 1105 24-25 0.3258567655722686 1105 26-27 0.17067567429307928 1105 28-29 0.07251686850607086 1105 30-31 -0.15267578050880815 1105 32-33 -0.037375511117936355 1105 34-35 -0.06581652936261406 1105 36-37 0.012256390666031791 1105 38-39 0.14669459010544728 1105 40-41 -0.0016269394074015509 1105 42-43 -0.17242551339152357 1105 44-45 -0.514234885306422 1105 46-47 -0.14923981462417402 1105 48-49 0.09979225561082217 1105 50-51 -0.31183943168061745 1105 52-53 -0.24826409242602665 1105 54-55 -0.5663091303780377 1105 56-57 -0.48350041490732565 1105 58-59 -0.3735362043307049 1105 60-61 -0.47039355692441376 1105 62-63 0.008448911197866948 1105 64-65 -0.5061835661762757 1105 66-67 -0.7470652525204144 1105 68-69 -0.8509669988358013 1105 70-71 -0.9141533432524582 1105 72-73 -0.6360010934096181 1105 74-75 -0.27829849094810655 1105 76-77 -0.7383707637130783 1105 78-79 -0.2956055146580283 1105 80-81 -0.4889027487435236 1105 82-83 -0.27467071630636397 1105 84-85 0.13223024289977658 1105 86-87 -0.008793995698876955 1105 88-89 0.19609643508284336 1105 90-91 -0.061923922611789806 1105 92-93 0.025562429422866018 1105 94-95 -0.169219197521425 1105 96-97 -0.029700323561723252 1105 98-99 0.4158003777250734 1105 100-101 1.0126672455982622 1106 1 -0.2783026038216718 1106 2 -0.3395940741741512 1106 3 -0.278171643315126 1106 4 0.003640776321176986 1106 5 0.004854768268046428 1106 6 -0.26136296480427035 1106 7 -0.5020685005081091 1106 8 -0.3798232059736577 1106 9 -0.2088909405270556 1106 10-11 -0.3930802036540513 1106 12-13 -0.43951649795349823 1106 14-15 -0.3073654547584326 1106 16-17 -0.2073051186257402 1106 18-19 -0.0873010730287973 1106 20-21 -0.12692351011088476 1106 22-23 -0.08980885626301927 1106 24-25 -0.17421991213598886 1106 26-27 -0.04876059119581555 1106 28-29 -0.2732382535648412 1106 30-31 -0.2633217135231618 1106 32-33 -0.29309566815387456 1106 34-35 -0.5612925445754726 1106 36-37 -0.268445363719934 1106 38-39 -0.47281528598337985 1106 40-41 -0.8376439925297205 1106 42-43 -1.1978058348244716 1106 44-45 -0.5889036376067551 1106 46-47 -0.41212166961129526 1106 48-49 -0.38140637032465463 1106 50-51 -0.7253929833920623 1106 52-53 -0.3027819429965035 1106 54-55 -0.2973083942720365 1106 56-57 -0.4055958633185668 1106 58-59 -0.35350798693406915 1106 60-61 -0.41742459606071236 1106 62-63 -0.6396157434457379 1106 64-65 -0.34946966604131546 1106 66-67 -0.027092243073724376 1106 68-69 0.16953662035203365 1106 70-71 0.08822366570350226 1106 72-73 -0.011108442201177127 1106 74-75 -0.19464311124009726 1106 76-77 -0.3273169052907967 1106 78-79 0.10429940498373114 1106 80-81 -0.1913625696243919 1106 82-83 -0.24978113220625175 1106 84-85 -0.019484270656842995 1106 86-87 0.3310109362113174 1106 88-89 0.5245990727960077 1106 90-91 0.7557671582371874 1106 92-93 1.1372971858944645 1106 94-95 0.3682148596494734 1106 96-97 0.10088181359936144 1106 98-99 0.6841570210817167 1106 100-101 0.2815299618293956 1107 1 0.11531627729721095 1107 2 0.17041466708458586 1107 3 0.24822695808347106 1107 4 0.03642049660089697 1107 5 0.04432155148482764 1107 6 0.2934010212097178 1107 7 0.19788779319818417 1107 8 0.1777379828375274 1107 9 0.23704437415825907 1107 10-11 -0.04211866519251117 1107 12-13 0.0389581523961553 1107 14-15 -0.0216137065066917 1107 16-17 0.3405095666889366 1107 18-19 0.21112550039777744 1107 20-21 -0.02591826535563513 1107 22-23 -0.07466462549379571 1107 24-25 -0.34294805898912983 1107 26-27 -0.07872125553148379 1107 28-29 -0.10094804377462907 1107 30-31 0.16821237738593453 1107 32-33 0.1266595766013623 1107 34-35 -0.056331880239802956 1107 36-37 0.07217963628006885 1107 38-39 -0.3518799712980609 1107 40-41 0.14437086761014228 1107 42-43 0.39067755678391336 1107 44-45 0.5930019567988367 1107 46-47 0.5196746590600441 1107 48-49 1.0269852380669562 1107 50-51 1.2743447788456947 1107 52-53 1.1221306444160888 1107 54-55 0.7491513959377514 1107 56-57 0.9722013394786302 1107 58-59 0.7982839711078853 1107 60-61 0.7599792500931315 1107 62-63 0.7807076831276873 1107 64-65 1.0108668724202161 1107 66-67 0.9964654492339662 1107 68-69 1.056818088883503 1107 70-71 1.0031712181510457 1107 72-73 0.9489221522044176 1107 74-75 0.734640105543118 1107 76-77 0.7199077450588547 1107 78-79 1.1897015028858284 1107 80-81 1.1232134793266546 1107 82-83 1.2781253363252176 1107 84-85 1.3043793657067937 1107 86-87 1.10979153061691 1107 88-89 1.7791008210477557 1107 90-91 2.1383502001952266 1107 92-93 2.0879527803000606 1107 94-95 2.0150461533557653 1107 96-97 1.85741590450845 1107 98-99 1.7439253777250734 1107 100-101 2.1542790877035216 1108 1 0.04611524636937503 1108 2 0.20578837842479203 1108 3 0.3232269580834739 1108 4 -0.018347544636213797 1108 5 0.17982412880441245 1108 6 -0.07947269013048697 1108 7 0.13184397876519682 1108 8 0.25228695190969574 1108 9 0.2604335494159926 1108 10-11 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0.5897313364695123 2316 82-83 0.48303605061092725 2316 84-85 0.5990222228496513 2316 86-87 0.07898795918833912 2316 88-89 0.4299936781906126 2316 90-91 0.7155823430523718 2316 92-93 0.6174170660143439 2316 94-95 1.1083497247843361 2316 96-97 0.8775051902227347 2316 98-99 1.5515146634393595 2316 100-101 0.36454694484637784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 4.0 6 9.0 7 15.0 8 45.0 9 94.0 10 159.0 11 255.0 12 412.0 13 580.0 14 773.0 15 1095.0 16 1591.0 17 2327.0 18 2216.0 19 1338.0 20 1129.0 21 1159.0 22 1259.0 23 1307.0 24 1521.0 25 1674.0 26 2098.0 27 2461.0 28 3015.0 29 3840.0 30 4981.0 31 6309.0 32 8397.0 33 11663.0 34 17320.0 35 26481.0 36 43924.0 37 68293.0 38 53385.0 39 4547.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.896804514689066 20.084559690794606 15.053802464207298 21.964833330309023 2 15.936708768926067 21.01691103388736 39.51022569809706 23.536154499089516 3 20.187682731302463 24.265628740777284 29.889944065177488 25.656744462742765 4 13.392073360950093 17.27667786330429 37.01165852915358 32.31959024659204 5 13.848040104759901 37.570281270177524 34.36110244560683 14.22057617945574 6 32.26481619860852 36.71203360442255 16.957102126393835 14.066048070575091 7 28.436073970356716 33.1049267623822 20.556591385602044 17.902407881659038 8 26.33869949723953 35.44098549757326 19.487227852784773 18.73308715240244 9 26.374973701202126 17.130130079295412 20.136536103715205 36.35836011578726 10-11 24.96100523074021 27.10136463555307 27.148521100704443 20.78910903300227 12-13 25.83070940406576 24.248552606758857 27.68946704006268 22.231270949112698 14-15 22.784915735024196 26.035809894151875 25.92735002430372 25.251924346520216 16-17 22.128534014321055 29.182778458926716 26.769637040315153 21.919050486437076 18-19 21.902908465673722 27.95979367232786 29.04040220837354 21.09689565362488 20-21 22.638912064074752 26.7787055913058 29.243175008524435 21.339207336095008 22-23 22.40571256524941 26.571336329636924 29.283970501282315 21.73898060383135 24-25 22.49327113516494 26.823141491159973 29.228302584899772 21.455284788775312 26-27 22.2253660606918 26.91446159650163 29.230805505828496 21.629366836978072 28-29 21.830205482309367 27.770855211966545 29.100350602263227 21.29858870346086 30-31 22.106406749903872 27.40135230232373 28.835090213945254 21.657150733827145 32-33 22.277076879547877 27.077786402977388 28.88496724439382 21.76016947308091 34-35 22.529001226068093 27.251358468938395 28.911991526345954 21.307648778647554 36-37 22.883218827762825 26.82169052300147 28.921241448356415 21.373849200879285 38-39 22.228650817257815 27.302323725505843 29.050740356502875 21.418285100733463 40-41 22.469330160549628 27.335333251111805 28.647733950478454 21.547602637860113 42-43 22.39361788964449 27.515957996774844 28.301749892525002 21.78867422105567 44-45 22.552398087623967 27.340774381706197 28.347202170648366 21.759625360021477 46-47 22.861091563345642 27.784770638208343 27.869652275480817 21.484485522965198 48-49 22.63546601469831 27.89214228193762 27.699889000935872 21.772502702428195 50-51 22.400409173020698 28.03361167739174 27.936759552811612 21.629219596775947 52-53 22.54822655416827 28.258148999920195 27.48877313387358 21.704851312037956 54-55 22.414193370526483 28.3225357119538 27.407518916997365 21.85575200052235 56-57 22.74138669026908 28.044493938580516 27.574198884205487 21.639920486944913 58-59 22.635647385718123 27.608659377969953 27.66742358838935 22.088269647922576 60-61 22.307547210876457 27.917715595731252 27.987362067339433 21.787375126052858 62-63 22.00175567147179 27.913725433295365 28.244727544454033 21.839791350778807 64-65 21.884771363692423 28.255791176662626 28.042136115322947 21.817301344321997 66-67 21.985613650708437 27.683384238132895 27.853510254717463 22.47749185644121 68-69 22.010642851442626 27.607933893890696 28.0428615994022 22.338561655264474 70-71 22.183308062304572 27.83464766865691 27.81505959851711 22.166984670521405 72-73 21.9194132284767 27.335151880091992 28.069885881354335 22.675549010076974 74-75 21.755997939625217 27.878720826471465 27.58363017723576 22.781651056667563 76-77 21.878786120038598 27.511807253389826 27.842627993528684 22.766778633042897 78-79 22.11783312415209 27.370337857935706 27.402259157422794 23.10956986048941 80-81 22.020980999571965 27.31919123034845 27.326627442160785 23.333200327918803 82-83 21.805007662939484 27.396867716806778 27.82744329878209 22.97068132147165 84-85 21.40051074079179 27.225603784124957 27.846799526984377 23.52708594809887 86-87 21.628312741676883 27.05529639652058 27.570752834829037 23.7456380269735 88-89 20.89285325633529 27.179172803052836 27.726731911868196 24.201242028743682 90-91 21.112844419452557 26.89691450960475 27.98690344464801 24.00333762629469 92-93 20.746924843204184 27.045419095535717 28.0068703591522 24.2007857021079 94-95 20.26875557715886 27.882892359927165 27.749221918325002 24.099130144588976 96-97 20.335137370410404 28.434804373218032 27.33442639601274 23.895631860358822 98-99 20.314174869275178 28.721553861528726 26.258685514982343 24.705585754213754 100-101 20.053476905885724 28.51690472476082 26.014483026343 25.415135343010455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29.0 1 20.0 2 12.0 3 20.0 4 25.0 5 24.0 6 32.5 7 69.5 8 128.0 9 180.5 10 188.0 11 206.0 12 244.5 13 253.5 14 297.0 15 311.5 16 299.0 17 326.0 18 374.0 19 427.0 20 462.5 21 496.5 22 563.0 23 656.0 24 806.5 25 999.0 26 1318.5 27 1773.0 28 2197.0 29 2626.5 30 3081.5 31 3730.0 32 4428.0 33 4946.5 34 5481.5 35 6114.0 36 6896.0 37 7592.0 38 8230.0 39 8587.5 40 8778.5 41 9303.0 42 9513.0 43 9723.0 44 10119.5 45 10316.0 46 10619.0 47 11517.5 48 12162.5 49 11527.5 50 10650.5 51 10079.0 52 9684.0 53 9537.5 54 9498.5 55 8742.0 56 7306.0 57 6351.5 58 5800.5 59 4966.0 60 3851.0 61 3020.5 62 2426.0 63 1693.0 64 1103.0 65 779.0 66 551.5 67 449.5 68 369.5 69 278.0 70 195.0 71 111.0 72 76.5 73 48.0 74 28.5 75 23.5 76 18.5 77 9.5 78 4.5 79 2.5 80 2.0 81 0.5 82 1.0 83 1.0 84 0.0 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04824469127025007 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.003627420396259404 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001813710198129702 24-25 0.0 26-27 0.002357823257568613 28-29 0.003446049376446434 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.011789116287843062 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.813710198129702E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.011970487307656034 92-93 3.627420396259404E-4 94-95 0.0 96-97 0.0 98-99 0.0012695971386907914 100-101 0.01632339178316732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 275678.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.28957298358351 #Duplication Level Percentage of deduplicated Percentage of total 1 86.53083222192117 63.41807743458712 2 8.247108146100167 12.088540687542288 3 2.321852798114761 5.105028003137082 4 0.965548682588088 2.8305860256697035 5 0.5346405758680934 1.9591789752534876 6 0.3503423263441402 1.5405863697502378 7 0.21284845043242723 1.0919700429687023 8 0.15255711810642794 0.8944676833300977 9 0.10435659069390896 0.6883424972981272 >10 0.5439410603420861 6.782007699419329 >50 0.02198290690977342 1.0340447358279483 >100 0.012490288016916675 1.65908523301573 >500 0.0014988345620300012 0.9080846122001789 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.3065170234839197 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 818 0.29672298841401923 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 817 0.2963602463743933 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 466 0.16903779046568823 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 441 0.15996923947503974 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.6274203962594043E-4 0.0 18-19 0.0 0.0 0.0 3.6274203962594043E-4 0.0 20-21 0.0 0.0 0.0 9.068550990648511E-4 0.0 22-23 0.0 0.0 0.0 0.001813710198129702 0.0 24-25 0.0 0.0 0.0 0.0030833073368204938 0.0 26-27 0.0 0.0 0.0 0.0034460493764464336 0.0 28-29 0.0 0.0 0.0 0.006529356713266927 0.0 30-31 0.0 0.0 0.0 0.015597907703915437 0.0 32-33 0.0 0.0 0.0 0.023034119516247217 3.6274203962594043E-4 34-35 0.0 0.0 0.0 0.034823235804090275 3.6274203962594043E-4 36-37 0.0 0.0 0.0 0.05042114350800572 3.6274203962594043E-4 38-39 0.0 0.0 0.0 0.07526897322238263 3.6274203962594043E-4 40-41 0.0 0.0 0.0 0.10755301474909132 3.6274203962594043E-4 42-43 3.6274203962594043E-4 0.0 0.0 0.15180754358345605 3.6274203962594043E-4 44-45 3.6274203962594043E-4 0.0 0.0 0.1960620724178208 3.6274203962594043E-4 46-47 3.6274203962594043E-4 0.0 0.0 0.2847525011063632 3.6274203962594043E-4 48-49 3.6274203962594043E-4 0.0 0.0 0.3823301097657412 3.6274203962594043E-4 50-51 3.6274203962594043E-4 0.0 0.0 0.4278542357387967 3.6274203962594043E-4 52-53 3.6274203962594043E-4 0.0 0.0 0.5136427281103316 3.6274203962594043E-4 54-55 3.6274203962594043E-4 0.0 0.0 0.669803176169299 3.6274203962594043E-4 56-57 3.6274203962594043E-4 0.0 0.0 0.9453057552652007 3.6274203962594043E-4 58-59 3.6274203962594043E-4 0.0 0.0 1.2917244031079738 3.6274203962594043E-4 60-61 3.6274203962594043E-4 0.0 0.0 1.6851181450823063 3.6274203962594043E-4 62-63 3.6274203962594043E-4 0.0 0.0 2.1068057661474615 3.6274203962594043E-4 64-65 3.6274203962594043E-4 0.0 0.0 2.5781890466413717 3.6274203962594043E-4 66-67 3.6274203962594043E-4 0.0 0.0 3.157669454943811 3.6274203962594043E-4 68-69 3.6274203962594043E-4 0.0 0.0 3.780860279021177 3.6274203962594043E-4 70-71 3.6274203962594043E-4 0.0 0.0 4.217420323710996 3.6274203962594043E-4 72-73 3.6274203962594043E-4 0.0 0.0 4.826826950282577 3.6274203962594043E-4 74-75 3.6274203962594043E-4 0.0 0.0 5.507512387640653 3.6274203962594043E-4 76-77 3.6274203962594043E-4 0.0 0.0 6.290309709153433 3.6274203962594043E-4 78-79 3.6274203962594043E-4 0.0 0.0 7.124979142332721 3.6274203962594043E-4 80-81 3.6274203962594043E-4 0.0 0.0 8.388772408389498 3.6274203962594043E-4 82-83 3.6274203962594043E-4 0.0 0.0 9.667800840110564 3.6274203962594043E-4 84-85 3.6274203962594043E-4 0.0 0.0 11.427462474336 3.6274203962594043E-4 86-87 3.6274203962594043E-4 0.0 0.0 13.166629183322572 3.6274203962594043E-4 88-89 3.6274203962594043E-4 0.0 0.0 15.155725157611416 3.6274203962594043E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATAC 65 1.3257304E-4 36.537693 3 GGTAAGG 135 3.0559022E-10 35.18445 3 GTAAGGT 125 5.6206773E-9 34.199284 4 ATATAAC 70 2.0495521E-4 33.92786 3 GTGTAGG 85 1.6219008E-5 33.546967 1 ACTGACC 100 1.2946603E-6 33.249302 8 GCGTAGG 60 0.0039356905 31.683239 1 CCCTATA 60 0.003946195 31.666002 2 TAAGGTG 170 3.616151E-9 27.940592 5 TAATCGG 35 0.008388709 27.142286 14-15 TACCTGG 215 5.638867E-11 26.511072 2 CCTATAG 90 8.9313276E-4 26.388334 3 AATCGGA 45 8.9427154E-4 26.388334 16-17 GTAGATC 110 9.452377E-5 25.908548 3 GAGTACT 925 0.0 25.675135 12-13 GTATGTA 170 1.1107295E-7 25.160223 1 GGTATCA 1565 0.0 24.901207 1 AGGTAAG 185 2.5276313E-7 23.107624 2 GTACTTT 1015 0.0 22.930552 14-15 GGTGTGC 250 3.783498E-10 22.799522 8 >>END_MODULE