FastQCFastQC Report
Thu 26 May 2016
SRR937290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258870
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7940.30671765751149227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6050.23370803878394558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.14988218024491057No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3830.14795070885000192No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3600.13906594043342219No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3570.137907057596477No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2690.10391316104608489No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2670.10314057248812145No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCTAT352.7785677E-454.3067361
GTATAGG702.044286E-433.941711
GGTATCA10150.031.3668211
CATGGGG3650.029.93734
GGCCGAC650.005835796729.230777
TAGACTG1004.9319387E-528.5055185
TACATGG6950.028.0270062
GTACATG7950.026.8972031
GTATCAA14100.026.6237831
GGACCAT1257.3619194E-626.5999986
ACGAGGT458.9459075E-426.38633528-29
GAGTACT9100.024.53296712-13
ACATGGG7350.023.9161933
TCAACGC15600.023.4500524
ATCAACG15650.023.3751323
CAACGCA15700.022.9980835
CCCCTAT1050.002186719322.6278081
GTACTTT10200.022.58578514-15
TATCAAC16200.022.5815322
AACGCAG16100.022.422366
Produced by FastQC (version 0.11.5)