##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937290_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 258870 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.018607795418546 33.0 31.0 34.0 31.0 34.0 2 32.15069726117356 33.0 31.0 34.0 31.0 34.0 3 32.19724185884807 34.0 31.0 34.0 30.0 34.0 4 35.76917371653726 37.0 35.0 37.0 35.0 37.0 5 35.763773322517096 37.0 35.0 37.0 35.0 37.0 6 35.794850697261175 37.0 36.0 37.0 35.0 37.0 7 35.74417275080156 37.0 35.0 37.0 35.0 37.0 8 35.73727353497895 37.0 35.0 37.0 35.0 37.0 9 37.41129138177464 39.0 37.0 39.0 35.0 39.0 10-11 37.32704446247151 39.0 37.0 39.0 35.0 39.0 12-13 37.20251477575617 39.0 37.0 39.0 34.0 39.0 14-15 38.477448139993044 40.0 38.0 41.0 34.0 41.0 16-17 38.453519140881525 40.0 38.0 41.0 34.0 41.0 18-19 38.40514157685325 40.0 38.0 41.0 34.0 41.0 20-21 38.34171978213003 40.0 38.0 41.0 34.0 41.0 22-23 38.1982925792869 40.0 38.0 41.0 34.0 41.0 24-25 38.04008382585854 40.0 38.0 41.0 33.5 41.0 26-27 37.89843743964152 40.0 38.0 41.0 33.0 41.0 28-29 37.93624985513964 40.0 38.0 41.0 33.0 41.0 30-31 37.805016031212574 40.0 38.0 41.0 33.0 41.0 32-33 37.60975199907289 40.0 37.0 41.0 33.0 41.0 34-35 37.50706532236258 40.0 37.0 41.0 33.0 41.0 36-37 37.585038822575044 40.0 37.5 41.0 33.0 41.0 38-39 37.49442191061151 40.0 37.0 41.0 33.0 41.0 40-41 37.33039363387029 40.0 37.0 41.0 32.5 41.0 42-43 37.10937343067949 40.0 37.0 41.0 31.5 41.0 44-45 36.906304322632984 40.0 37.0 41.0 31.0 41.0 46-47 36.59117897013945 40.0 36.5 41.0 30.5 41.0 48-49 36.30754819021131 39.5 36.0 41.0 30.0 41.0 50-51 35.43743771004752 38.5 35.0 40.0 28.5 40.5 52-53 35.50256499401244 38.5 35.0 40.0 28.5 40.5 54-55 35.808496542666205 39.0 35.5 40.5 28.5 41.0 56-57 35.632564607718166 39.0 35.0 41.0 28.0 41.0 58-59 35.41591146135126 39.0 35.0 40.5 28.0 41.0 60-61 35.121024066133586 38.5 35.0 40.0 26.5 41.0 62-63 34.806095723722336 38.0 34.5 40.0 26.0 41.0 64-65 34.439633793023525 38.0 34.0 40.0 26.0 41.0 66-67 34.04089118090161 37.0 34.0 40.0 25.5 41.0 68-69 33.595918800942556 36.5 33.5 39.0 23.5 41.0 70-71 33.16244833314019 36.0 33.0 39.0 22.5 40.5 72-73 32.76457874608877 35.5 33.0 39.0 21.5 40.0 74-75 32.312371074284385 35.0 32.0 37.5 20.0 40.0 76-77 31.887569050102368 35.0 32.0 37.0 19.5 39.0 78-79 31.424454359330937 35.0 31.5 36.5 18.0 39.0 80-81 30.968140379340984 35.0 31.0 36.0 13.5 38.5 82-83 30.507642832309656 34.5 31.0 36.0 7.5 37.0 84-85 30.101189786379265 34.0 30.5 35.0 7.0 37.0 86-87 29.68464673388187 34.0 30.0 35.0 2.0 36.5 88-89 29.29707575230811 34.0 29.5 35.0 2.0 36.0 90-91 28.92297098929965 34.0 29.0 35.0 2.0 36.0 92-93 28.52273921273226 34.0 29.0 35.0 2.0 35.0 94-95 28.03329663537683 34.0 29.0 35.0 2.0 35.0 96-97 27.45497740178468 34.0 27.0 35.0 2.0 35.0 98-99 26.739869432533705 33.0 25.5 35.0 2.0 35.0 100-101 24.909116544983966 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0447590901737378 1101 2 0.002233560078614971 1101 3 -0.06951741209734408 1101 4 0.07318136194081859 1101 5 0.12539193825882222 1101 6 0.10430943596851705 1101 7 0.22207658434261646 1101 8 0.0024330688692018043 1101 9 0.2673210884764927 1101 10-11 0.15756805872307922 1101 12-13 0.09662017537991119 1101 14-15 0.1542736646379339 1101 16-17 0.244639394174051 1101 18-19 0.16310213002593343 1101 20-21 0.19405579008267893 1101 22-23 0.3686925511700423 1101 24-25 0.19808365367740777 1101 26-27 -0.009547177338291135 1101 28-29 0.04759172517352539 1101 30-31 0.017731910051928423 1101 32-33 0.38624531088179737 1101 34-35 0.44973630008519905 1101 36-37 0.3632617532272704 1101 38-39 0.27012184271294615 1101 40-41 0.40074039260372274 1101 42-43 0.4366538262113977 1101 44-45 0.6048802317723982 1101 46-47 0.5692617201086065 1101 48-49 0.8028082647912456 1101 50-51 0.9122304704817452 1101 52-53 0.7983578231903579 1101 54-55 0.9196755246959398 1101 56-57 0.9854829744748201 1101 58-59 0.9849882548225253 1101 60-61 0.9630320441348985 1101 62-63 0.8384124063657836 1101 64-65 0.8605874936294953 1101 66-67 0.7903623078287936 1101 68-69 0.8260288073690063 1101 70-71 1.0661055625587608 1101 72-73 0.9084552455211323 1101 74-75 0.9331315919493761 1101 76-77 0.8253618728164653 1101 78-79 0.8639500738107309 1101 80-81 0.9420200425203724 1101 82-83 0.9438488874490645 1101 84-85 1.1237992009352453 1101 86-87 1.2972242718196263 1101 88-89 1.3162313748950218 1101 90-91 1.297570676579138 1101 92-93 1.301421091012056 1101 94-95 1.546125054687792 1101 96-97 1.696031151461213 1101 98-99 1.5194331517620476 1101 100-101 1.7765152810192184 1102 1 0.334534624867036 1102 2 0.33310106907840265 1102 3 0.4720016109844636 1102 4 0.3565899876202181 1102 5 0.4107416947351439 1102 6 0.13718513301094504 1102 7 0.3106839112290416 1102 8 0.40472360320956824 1102 9 0.2990285777120363 1102 10-11 0.5541904911364313 1102 12-13 0.5585510711235386 1102 14-15 0.4168398395514643 1102 16-17 0.5568967528244286 1102 18-19 0.395024908313502 1102 20-21 0.2852465855933701 1102 22-23 0.47202070815458086 1102 24-25 0.4320075613501686 1102 26-27 0.6002396512069197 1102 28-29 0.3673506720218356 1102 30-31 0.3676542648200538 1102 32-33 0.33834349680683573 1102 34-35 0.44336939107129325 1102 36-37 0.3933863385311511 1102 38-39 0.6727529572749589 1102 40-41 0.6943999535552265 1102 42-43 0.7567427652951864 1102 44-45 0.5670017112669967 1102 46-47 0.9301814631734757 1102 48-49 0.8579999073408331 1102 50-51 0.7184138544021224 1102 52-53 0.9305753004261916 1102 54-55 0.9382168531352661 1102 56-57 0.83817585228946 1102 58-59 0.9220832643517127 1102 60-61 0.6501429356433377 1102 62-63 0.6665111569834181 1102 64-65 0.6657244315838966 1102 66-67 0.7208627666415239 1102 68-69 1.0591092054772915 1102 70-71 1.0186484439027268 1102 72-73 0.81041931695475 1102 74-75 0.6677807766744408 1102 76-77 0.7234366239987864 1102 78-79 0.6357772072899408 1102 80-81 0.5023680343323313 1102 82-83 0.6930318478499942 1102 84-85 0.7015820766322847 1102 86-87 0.6533247165150442 1102 88-89 0.4793851830407121 1102 90-91 0.6202042616712511 1102 92-93 1.0152242956425326 1102 94-95 0.8816477482714724 1102 96-97 0.9786103848910166 1102 98-99 1.2586334058737165 1102 100-101 1.6221265607538164 1103 1 0.17993261932837967 1103 2 0.28537019789661144 1103 3 0.31853224259275237 1103 4 -0.08952079733883522 1103 5 0.08503438952205045 1103 6 0.0055027220949313005 1103 7 0.13921094923465205 1103 8 0.3192658339710448 1103 9 0.16673537294191476 1103 10-11 0.4416532060261673 1103 12-13 0.4160077966754727 1103 14-15 0.6299861395046165 1103 16-17 0.4415753151461601 1103 18-19 0.31652900300434084 1103 20-21 0.005953683255157216 1103 22-23 -0.09905375532528637 1103 24-25 0.2274568506351855 1103 26-27 0.4330570712848427 1103 28-29 0.3184130382253656 1103 30-31 0.12809557820474282 1103 32-33 0.2505581878815306 1103 34-35 0.1708308013527855 1103 36-37 0.4427420399656228 1103 38-39 0.25156908460471783 1103 40-41 0.715249965118808 1103 42-43 0.7235582759010555 1103 44-45 0.5815718132043415 1103 46-47 0.7997058148721337 1103 48-49 0.8425669849590847 1103 50-51 1.0615791897753866 1103 52-53 1.0326549645488612 1103 54-55 1.0709103354311438 1103 56-57 0.8585662859530956 1103 58-59 1.1662999953592816 1103 60-61 1.3156502799946779 1103 62-63 1.2772160605313587 1103 64-65 1.2657256175913787 1103 66-67 1.1314585575009701 1103 68-69 1.1551965667885398 1103 70-71 1.048138519985109 1103 72-73 0.9728074616239439 1103 74-75 0.964137361684493 1103 76-77 1.1815053294123175 1103 78-79 0.9352915372253641 1103 80-81 1.0834790861430683 1103 82-83 0.9916655672287646 1103 84-85 0.5081116408338353 1103 86-87 0.6765734282144145 1103 88-89 0.33448471871878027 1103 90-91 0.4210736051568098 1103 92-93 0.672008554951212 1103 94-95 0.2920774980831915 1103 96-97 0.20325206238000249 1103 98-99 0.763401155958217 1103 100-101 0.8753154383756332 1104 1 0.15852799850251031 1104 2 0.23631411411543013 1104 3 0.17382138870455321 1104 4 0.2589932810222848 1104 5 0.23174331423841465 1104 6 0.12194202208115001 1104 7 0.017374561379249087 1104 8 0.18454533422367092 1104 9 0.290776528607708 1104 10-11 0.004925448918086772 1104 12-13 -0.10218153776024508 1104 14-15 0.16339066846516204 1104 16-17 -0.04345063680828076 1104 18-19 0.052048731082976474 1104 20-21 -0.09396134131881695 1104 22-23 0.14527356082923149 1104 24-25 0.041395117136495685 1104 26-27 0.07075171094310662 1104 28-29 -0.03263881975083649 1104 30-31 0.18254874985510838 1104 32-33 0.14214562070501557 1104 34-35 0.4342086893689583 1104 36-37 0.29537857480554663 1104 38-39 0.4139754968044329 1104 40-41 0.24487653257092035 1104 42-43 0.3058810906546299 1104 44-45 0.6328326647959059 1104 46-47 0.6475194201268337 1104 48-49 0.47802240730992196 1104 50-51 0.7078747205273075 1104 52-53 0.9459111310088346 1104 54-55 1.2398277025709135 1104 56-57 0.9459531767711553 1104 58-59 0.7842091405524343 1104 60-61 0.8703675644556554 1104 62-63 0.5107606841131869 1104 64-65 0.37888073219337315 1104 66-67 0.5253541066259473 1104 68-69 1.0796373366199603 1104 70-71 1.3139049064625326 1104 72-73 1.1626241902997023 1104 74-75 1.005580106620414 1104 76-77 0.8154794233905491 1104 78-79 0.7126788873700498 1104 80-81 1.4778684113922544 1104 82-83 1.1708718508170257 1104 84-85 1.3480018617699443 1104 86-87 1.0779560013621783 1104 88-89 0.8555333614086003 1104 90-91 0.8595353202824363 1104 92-93 0.675660201637168 1104 94-95 0.9840875114036649 1104 96-97 1.2666161705973558 1104 98-99 1.4365098785708703 1104 100-101 0.9664688075364403 1105 1 0.45122340310236453 1105 2 0.6142223840562622 1105 3 0.49276351246450645 1105 4 0.6159931475643887 1105 5 0.5765095404940297 1105 6 0.5370628014752512 1105 7 0.7258357918610301 1105 8 0.47163550727407255 1105 9 0.35170005723443154 1105 10-11 0.555383654354273 1105 12-13 0.5359585040565662 1105 14-15 0.507912221915042 1105 16-17 0.7295263639479899 1105 18-19 0.913775231372135 1105 20-21 0.6831083681878312 1105 22-23 0.7891522920895184 1105 24-25 0.6422670420441889 1105 26-27 0.6674530766622198 1105 28-29 0.9178038155977077 1105 30-31 0.5262028269937673 1105 32-33 0.636785061961298 1105 34-35 0.6904144814415574 1105 36-37 0.6339056445150604 1105 38-39 0.718515289499841 1105 40-41 0.46685037482368585 1105 42-43 0.664860582624911 1105 44-45 0.4186661093441515 1105 46-47 0.8737194290240211 1105 48-49 0.9605171802090737 1105 50-51 0.6385322230852495 1105 52-53 0.7160299085345301 1105 54-55 1.194040523425933 1105 56-57 1.3487291009670699 1105 58-59 1.39204311893225 1105 60-61 1.5951885084478263 1105 62-63 1.6650380096169926 1105 64-65 1.6972334169213426 1105 66-67 1.6374921020883804 1105 68-69 1.353092561496517 1105 70-71 1.328151536650715 1105 72-73 1.5777783341673128 1105 74-75 1.7678471113804122 1105 76-77 1.7764096249119632 1105 78-79 1.7358004675115133 1105 80-81 1.4963634512071913 1105 82-83 1.5620035737584388 1105 84-85 1.3937890409149247 1105 86-87 1.8249420772552334 1105 88-89 1.8100464625251966 1105 90-91 2.507629096695979 1105 92-93 2.1860921603541925 1105 94-95 2.014971892381464 1105 96-97 1.9557593709176544 1105 98-99 2.1786914702786824 1105 100-101 2.4614975009836577 1106 1 -0.23245387420479702 1106 2 -0.0235728915342861 1106 3 0.09958517160545455 1106 4 0.11524679536078963 1106 5 0.24229302371976758 1106 6 0.06322244621160422 1106 7 -0.02652454698286988 1106 8 0.07149743211640924 1106 9 0.1467890597347079 1106 10-11 -0.5097075872026195 1106 12-13 -0.5217065330744575 1106 14-15 -0.6736845986245683 1106 16-17 -0.32839989645430023 1106 18-19 -0.40287402040978293 1106 20-21 -0.08186532289698789 1106 22-23 0.005911630821472613 1106 24-25 -0.27026980909586484 1106 26-27 0.1979975405947556 1106 28-29 0.3454169812505725 1106 30-31 0.33810341288025114 1106 32-33 0.2700742361225892 1106 34-35 0.4159341291633112 1106 36-37 0.5333160262742069 1106 38-39 0.1456339968178213 1106 40-41 0.8241198452868019 1106 42-43 0.6240257876851842 1106 44-45 0.3580716398139785 1106 46-47 0.9527581273857777 1106 48-49 0.992106537226654 1106 50-51 0.9786729104756304 1106 52-53 0.7357336067096938 1106 54-55 1.1942550996844759 1106 56-57 1.3088738932945674 1106 58-59 1.0773250534771677 1106 60-61 0.5124740254833213 1106 62-63 0.9363370356273606 1106 64-65 0.8090089888137229 1106 66-67 1.3701207545494825 1106 68-69 1.3183312076136176 1106 70-71 0.8484056695760387 1106 72-73 1.0571137075300072 1106 74-75 0.4289005875157983 1106 76-77 0.4903328252702188 1106 78-79 0.37188846243632057 1106 80-81 0.060251199835771985 1106 82-83 0.3661887437401532 1106 84-85 0.04878773479856591 1106 86-87 -0.07915166458153777 1106 88-89 -0.005707166612392456 1106 90-91 0.37290319454275433 1106 92-93 0.4959402776807593 1106 94-95 0.6867075344309477 1106 96-97 0.6582540531582026 1106 98-99 0.7672928062491273 1106 100-101 0.8364182010620276 1107 1 0.21726557886487186 1107 2 0.16033515444272695 1107 3 0.2383060955036953 1107 4 0.32922511425820744 1107 5 0.09166928345719327 1107 6 0.2230524837994423 1107 7 0.026043955495751447 1107 8 0.3193592831813987 1107 9 0.3899388118728595 1107 10-11 0.19268114734006048 1107 12-13 0.20217264108254085 1107 14-15 0.34968523304727483 1107 16-17 0.6479578962133488 1107 18-19 0.39332089161288053 1107 20-21 0.42084481492509695 1107 22-23 0.4348215834281035 1107 24-25 0.6483988186755525 1107 26-27 0.7415095523613786 1107 28-29 0.8900184956592554 1107 30-31 0.7090926812792233 1107 32-33 0.5498993709485376 1107 34-35 0.6913195407229367 1107 36-37 -0.03810087191177303 1107 38-39 0.3199271819787981 1107 40-41 0.748821593938537 1107 42-43 0.6249927212799946 1107 44-45 0.606873186907734 1107 46-47 0.5562472595969297 1107 48-49 1.0400718910426363 1107 50-51 1.2756713306968024 1107 52-53 0.6033726544843816 1107 54-55 1.0700926548405434 1107 56-57 0.9945115247158256 1107 58-59 0.9428395710998672 1107 60-61 0.7484602977500217 1107 62-63 0.4713100704369708 1107 64-65 0.6377936486165154 1107 66-67 0.4631098050479281 1107 68-69 1.0217277321001959 1107 70-71 0.8099598450949159 1107 72-73 0.8235924349909141 1107 74-75 0.6205713398173742 1107 76-77 1.0610960218709664 1107 78-79 1.2199845565693472 1107 80-81 1.3362358378485162 1107 82-83 0.6968800331665292 1107 84-85 1.0665874842129597 1107 86-87 1.1257837823869217 1107 88-89 0.161299969629912 1107 90-91 0.5664805764679031 1107 92-93 0.9065193483350065 1107 94-95 1.2956986549844167 1107 96-97 1.157859108450463 1107 98-99 1.419414612644509 1107 100-101 1.8407625928471774 1108 1 -0.14662564625427166 1108 2 -0.09674362324160413 1108 3 -0.2065355080847553 1108 4 -0.34290833121417563 1108 5 -0.6317677784996292 1108 6 -0.14490378197011466 1108 7 -0.1704096469713079 1108 8 -0.1346637053243498 1108 9 -0.3122619170309804 1108 10-11 -0.2639701002068975 1108 12-13 -0.2222035854383435 1108 14-15 -0.17827944315120448 1108 16-17 -0.13193416994869267 1108 18-19 -0.17466340891978405 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-1.5834802383498356 2316 74-75 -1.2902555048248203 2316 76-77 -1.631896191699667 2316 78-79 -2.545727345544112 2316 80-81 -2.6958831832860746 2316 82-83 -2.656151112550937 2316 84-85 -2.2479749926131447 2316 86-87 -3.0253563733416655 2316 88-89 -3.112800883169495 2316 90-91 -2.210493839549894 2316 92-93 -2.0784176186653625 2316 94-95 -2.424036235634791 2316 96-97 -2.8313882033774966 2316 98-99 -3.424115235334728 2316 100-101 -3.2412266544646506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1065.0 3 224.0 4 195.0 5 186.0 6 231.0 7 214.0 8 280.0 9 294.0 10 376.0 11 479.0 12 676.0 13 882.0 14 1032.0 15 1338.0 16 1684.0 17 2299.0 18 1965.0 19 1345.0 20 1356.0 21 1406.0 22 1483.0 23 1677.0 24 1835.0 25 2064.0 26 2355.0 27 2716.0 28 3167.0 29 3850.0 30 4758.0 31 5953.0 32 8096.0 33 10933.0 34 15427.0 35 22994.0 36 37058.0 37 56836.0 38 51946.0 39 8193.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65859950574436 18.95834463097387 15.176281927132154 23.20677393614961 2 14.780167657251273 19.61163920779045 41.36913635219639 24.239056782761892 3 18.9133094806623 23.846862754209067 30.55968588820222 26.68014187692641 4 12.376471499380422 16.19462515489467 38.18308550185874 33.24581784386617 5 13.470593702080203 36.40704140270442 35.68563064388718 14.4367342513282 6 31.612253981769978 35.74052212954541 18.215315541965822 14.431908346718789 7 27.634690331244748 32.207253404750205 22.061310659509502 18.09674560449555 8 25.49211607979131 34.49844024553554 20.963409631000022 19.046034043673124 9 25.168641079922132 16.766967634071108 21.483886697963147 36.58050458804361 10-11 24.21059558301254 26.106133887634098 28.61422873035319 21.069041799000168 12-13 25.050026513084305 23.45982203334069 28.925000870867734 22.56515058270728 14-15 22.15537227187051 25.074794966966362 27.132091975554722 25.637740785608404 16-17 21.363604697067043 28.266776068799494 28.10847840727008 22.261140826863386 18-19 21.31010180564237 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29.701284301413178 22.461245522747635 44-45 21.46247636813273 26.402092956203887 29.80662988770872 22.328800787954666 46-47 21.867199201198204 26.64046565810199 29.39079752464327 22.101537616056536 48-49 21.597521494379436 26.88118150873805 29.174302339757297 22.34699465712522 50-51 21.521831259832922 26.992079479989705 29.40558364730948 22.080505612867892 52-53 21.79657382607484 27.00918084562865 28.815160006812096 22.379085321484418 54-55 21.458427670765264 26.813504088922947 29.023805930002595 22.704262310309193 56-57 21.470158567379045 26.959163336977447 29.0401787873804 22.53049930826311 58-59 21.821112349462865 26.62959351103681 29.00297204420916 22.546322095291167 60-61 21.563934273971967 26.542146854175215 29.530080570884575 22.363838300968244 62-63 21.30795115258914 26.43205306573425 29.83844595418282 22.421549827493795 64-65 21.20809316778599 26.507117413622634 29.623803525717303 22.660985892874073 66-67 21.070839815180292 26.291725680497187 29.471893937634956 23.165540566687564 68-69 21.20719612099823 26.19042090884114 29.660393859537105 22.941989110623524 70-71 21.21679463760488 26.18985881915849 29.505944456484723 23.08740208675191 72-73 21.048231984117464 26.090279685909668 29.591287824549045 23.270200505423823 74-75 21.025327453457972 26.579216743325638 29.260377986458742 23.135077816757647 76-77 21.261815613541152 26.157954314573477 29.275631536682823 23.304598535202548 78-79 21.09547637568257 25.897933041536536 29.565903892816927 23.44068668996397 80-81 21.214173484314895 25.640097833574565 29.340203871546993 23.80552481056355 82-83 21.115344353523255 25.544446787884883 29.84758392365458 23.49262493493728 84-85 20.70161792761312 25.483617563859962 29.682838203368973 24.13192630515794 86-87 21.12995443420047 25.28853502568518 29.892905859703774 23.688604680410577 88-89 20.56299168417672 24.853437967982725 29.99849084640299 24.585079501437566 90-91 20.32735054642271 24.919700628463517 30.362450078944924 24.39049874616885 92-93 20.19046955660297 25.11841463697787 30.431594263468696 24.25952154295046 94-95 19.62428942368343 25.632795056052814 30.26871297166982 24.47420254859394 96-97 19.631831805103815 26.133208469338985 30.057330374263053 24.17762935129414 98-99 20.075346401890044 26.379367392074116 29.035647323113 24.50963888292284 100-101 20.07924899197449 26.128949873464748 28.889701500622998 24.90209963393776 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 326.0 1 176.5 2 30.5 3 35.0 4 33.0 5 30.5 6 41.0 7 77.0 8 150.0 9 176.5 10 179.0 11 199.5 12 209.0 13 226.0 14 242.0 15 279.0 16 324.5 17 338.0 18 358.5 19 381.0 20 419.5 21 500.5 22 595.0 23 728.0 24 876.5 25 1068.5 26 1388.0 27 1760.5 28 2156.5 29 2539.5 30 2978.0 31 3624.5 32 4352.0 33 4909.5 34 5398.5 35 5996.5 36 6672.0 37 7249.0 38 7688.0 39 7953.5 40 8322.5 41 8820.5 42 9058.0 43 9407.5 44 9751.0 45 9955.0 46 10258.0 47 10817.5 48 11394.0 49 11077.0 50 10087.0 51 9388.0 52 8949.5 53 8599.0 54 8276.0 55 7458.5 56 6506.0 57 5693.0 58 5072.5 59 4341.5 60 3307.0 61 2598.5 62 2089.5 63 1530.5 64 1011.5 65 712.0 66 506.0 67 361.0 68 284.5 69 206.5 70 152.0 71 111.0 72 72.5 73 45.5 74 35.5 75 28.5 76 19.0 77 11.0 78 10.5 79 7.5 80 4.0 81 4.0 82 2.0 83 2.0 84 2.5 85 1.0 86 1.0 87 2.0 88 2.0 89 1.5 90 2.0 91 1.5 92 0.5 93 0.5 94 0.0 95 0.5 96 1.0 97 1.5 98 1.5 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2719511724031367 2 0.23370803878394558 3 0.2387298644107081 4 0.2433653957584888 5 0.24182021864256192 6 0.19507861088577277 7 0.1858075481902113 8 0.1916019623749372 9 0.1858075481902113 10-11 0.20338393788387993 12-13 0.19585119944373625 14-15 0.19198825665391897 16-17 0.1916019623749372 18-19 0.18754587244562906 20-21 0.18542125391122957 22-23 0.18542125391122957 24-25 0.18059257542395799 26-27 0.17808166261057676 28-29 0.18291034109784834 30-31 0.19140881523544637 32-33 0.18658013674817475 34-35 0.1948854637462819 36-37 0.18291034109784834 38-39 0.18406922393479352 40-41 0.18117201684243056 42-43 0.18561440105072044 44-45 0.1852281067717387 46-47 0.18658013674817475 48-49 0.18870475528257427 50-51 0.18947734384053774 52-53 0.19546490516475448 54-55 0.18889790242206514 56-57 0.17866110402904933 58-59 0.17846795688955847 60-61 0.1792405454475219 62-63 0.18291034109784834 64-65 0.1755707497971955 66-67 0.17615019121566808 68-69 0.17499130837872293 70-71 0.18464866535326613 72-73 0.18271719395835748 74-75 0.18329663537683008 76-77 0.1825240468188666 78-79 0.181944605400394 80-81 0.18233089967937574 82-83 0.1798199868659945 84-85 0.17499130837872293 86-87 0.17479816123923206 88-89 0.17267354270483254 90-91 0.17846795688955847 92-93 0.17615019121566808 94-95 0.17499130837872293 96-97 0.17595704407617724 98-99 0.17904739830803107 100-101 0.17151465986788736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 258870.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.52360133206322 #Duplication Level Percentage of deduplicated Percentage of total 1 88.52456869612882 68.62743371691535 2 7.193803239381371 11.153790687822127 3 1.915727116552983 4.4554219573402944 4 0.8305464379933464 2.5754780378704543 5 0.4312971898285941 1.6717855699955562 6 0.2856346765361756 1.3286057274241985 7 0.17175182535422365 0.9320374025770487 8 0.13957614737658813 0.8656356483750336 9 0.09307992786077046 0.6494302097546016 >10 0.38759280202360735 5.021907241862102 >50 0.017946978767683495 0.9476397153476116 >100 0.007477907819868109 1.2301538392950424 >500 9.970543759824145E-4 0.5406802454205982 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 794 0.30671765751149227 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 605 0.23370803878394558 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 388 0.14988218024491057 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 383 0.14795070885000192 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 360 0.13906594043342219 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 357 0.137907057596477 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 269 0.10391316104608489 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 267 0.10314057248812145 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.9314713949086413E-4 1.9314713949086413E-4 18-19 0.0 0.0 0.0 7.725885579634565E-4 3.8629427898172826E-4 20-21 0.0 0.0 0.0 7.725885579634565E-4 3.8629427898172826E-4 22-23 0.0 0.0 0.0 9.657356974543207E-4 3.8629427898172826E-4 24-25 0.0 0.0 0.0 0.0019314713949086413 3.8629427898172826E-4 26-27 0.0 0.0 0.0 0.003476648510835554 7.725885579634565E-4 28-29 0.0 0.0 0.0 0.008112179858616293 7.725885579634565E-4 30-31 0.0 0.0 0.0 0.01892841967010469 7.725885579634565E-4 32-33 0.0 0.0 0.0 0.030324100900065667 7.725885579634565E-4 34-35 0.0 0.0 0.0 0.03785683934020938 7.725885579634565E-4 36-37 0.0 0.0 0.0 0.05002510912813381 7.725885579634565E-4 38-39 0.0 0.0 0.0 0.0732027658670375 7.725885579634565E-4 40-41 0.0 0.0 0.0 0.10198168965117627 7.725885579634565E-4 42-43 0.0 0.0 0.0 0.13771391045698614 7.725885579634565E-4 44-45 0.0 0.0 0.0 0.17190095414686907 7.725885579634565E-4 46-47 0.0 0.0 0.0 0.23969560010816238 7.725885579634565E-4 48-49 0.0 0.0 0.0 0.3356897284351219 7.725885579634565E-4 50-51 0.0 0.0 0.0 0.3776026577046394 7.725885579634565E-4 52-53 0.0 0.0 0.0 0.45293004210607646 7.725885579634565E-4 54-55 0.0 0.0 0.0 0.5860084212152818 7.725885579634565E-4 56-57 0.0 0.0 0.0 0.83149843550817 7.725885579634565E-4 58-59 0.0 0.0 0.0 1.1036427550507977 7.725885579634565E-4 60-61 0.0 0.0 0.0 1.4408776606018465 7.725885579634565E-4 62-63 0.0 0.0 0.0 1.818287171166995 7.725885579634565E-4 64-65 0.0 0.0 0.0 2.252868235021439 7.725885579634565E-4 66-67 0.0 0.0 0.0 2.756595974813613 7.725885579634565E-4 68-69 0.0 0.0 0.0 3.320585622126936 7.725885579634565E-4 70-71 0.0 0.0 0.0 3.7341136477768764 7.725885579634565E-4 72-73 0.0 0.0 0.0 4.28709390813922 7.725885579634565E-4 74-75 0.0 0.0 0.0 4.921195967087728 7.725885579634565E-4 76-77 0.0 0.0 0.0 5.645111445899486 7.725885579634565E-4 78-79 0.0 0.0 0.0 6.451887047552826 7.725885579634565E-4 80-81 0.0 0.0 0.0 7.646115811024838 7.725885579634565E-4 82-83 0.0 0.0 0.0 8.834936454591109 7.725885579634565E-4 84-85 0.0 0.0 0.0 10.599915015258624 7.725885579634565E-4 86-87 0.0 0.0 0.0 12.31776567389037 7.725885579634565E-4 88-89 0.0 0.0 0.0 14.402209603275775 7.725885579634565E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCTAT 35 2.7785677E-4 54.306736 1 GTATAGG 70 2.044286E-4 33.94171 1 GGTATCA 1015 0.0 31.366821 1 CATGGGG 365 0.0 29.9373 4 GGCCGAC 65 0.0058357967 29.23077 7 TAGACTG 100 4.9319387E-5 28.505518 5 TACATGG 695 0.0 28.027006 2 GTACATG 795 0.0 26.897203 1 GTATCAA 1410 0.0 26.623783 1 GGACCAT 125 7.3619194E-6 26.599998 6 ACGAGGT 45 8.9459075E-4 26.386335 28-29 GAGTACT 910 0.0 24.532967 12-13 ACATGGG 735 0.0 23.916193 3 TCAACGC 1560 0.0 23.450052 4 ATCAACG 1565 0.0 23.375132 3 CAACGCA 1570 0.0 22.998083 5 CCCCTAT 105 0.0021867193 22.627808 1 GTACTTT 1020 0.0 22.585785 14-15 TATCAAC 1620 0.0 22.581532 2 AACGCAG 1610 0.0 22.42236 6 >>END_MODULE