FastQCFastQC Report
Thu 26 May 2016
SRR937290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258870
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8580.33144049136632286No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8370.3233283115077066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.2974465948159308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.18658013674817475No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC4250.16417506856723452No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG352.785689E-454.2786527
TCACGAG250.001614495538.0023926-27
CGTTTGT250.001614495538.0023924-25
TGCTCGG550.002577594234.5409626
CTCACGA352.0510187E-433.9307124-25
ACGAGGT404.4936896E-429.68363828-29
GTGCATA1004.929871E-528.5073031
CGATTCG350.00839271127.13932628-29
ACGATTC350.00839271127.13932628-29
GGTAAGG1606.2105755E-826.7152753
GTATAAT908.915397E-426.395651
GTAAGGT1901.1836164E-824.9967464
TATACTG1351.3388561E-524.6264255
AGGTAAG1801.9413346E-723.7469082
ATTCGCC500.001653674923.74690830-31
TATGCAC1000.001651449123.7469083
TATAGGC1000.001651449123.7469084
AGAGTAC13700.023.22690410-11
GGTGTGC2052.6528141E-823.1677158
TAAGGTG2052.6528141E-823.1677155
Produced by FastQC (version 0.11.5)