FastQCFastQC Report
Thu 26 May 2016
SRR937289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322508
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.2896052190953403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.22914160268892553No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4480.13891128282089127No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4370.135500514715914No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT4300.1333300259218376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.13115953712776118No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT3920.12154737246827985No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGT753.0664937E-431.6790961
CGACAGC804.480308E-429.694548
GGACGAT700.00837306527.1492927
GCGTGCC908.921144E-426.3951459
TACATGG8850.025.7688222
GTACATG9450.025.6449831
CATGGGG4500.025.3432774
GTGCTAT950.001221837525.0098111
GTATCAA15200.025.0098111
GTTACAC1151.276896E-424.7884863
ACATGGG9400.024.766513
GGTATCA11250.024.49851
CTCTATA1000.001648791523.7556322
CATGGGA3950.022.857074
GTCTAGA1703.1266482E-622.3617171
GAGTACT9900.022.07422612-13
CGGTGGT550.00287593921.59099694-95
ATCAACG17800.021.3533763
TATCAAC17950.021.1749362
CAACGCA17800.021.0897355
Produced by FastQC (version 0.11.5)