##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937289_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 322508 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94538740124276 33.0 31.0 34.0 30.0 34.0 2 32.06995795453136 33.0 31.0 34.0 30.0 34.0 3 32.13079365473104 34.0 31.0 34.0 30.0 34.0 4 35.70582435164399 37.0 35.0 37.0 35.0 37.0 5 35.680783112356906 37.0 35.0 37.0 35.0 37.0 6 35.73401279968249 37.0 35.0 37.0 35.0 37.0 7 35.684094658117004 37.0 35.0 37.0 35.0 37.0 8 35.670141515869375 37.0 35.0 37.0 35.0 37.0 9 37.34599761866372 39.0 37.0 39.0 35.0 39.0 10-11 37.25741531993005 39.0 37.0 39.0 34.0 39.0 12-13 37.164698240043656 39.0 37.0 39.0 34.0 39.0 14-15 38.41978493556749 40.0 38.0 41.0 34.0 41.0 16-17 38.36772266114329 40.0 38.0 41.0 34.0 41.0 18-19 38.30971789847074 40.0 38.0 41.0 34.0 41.0 20-21 38.21798529028737 40.0 38.0 41.0 34.0 41.0 22-23 38.09336977687375 40.0 38.0 41.0 34.0 41.0 24-25 37.954675542932264 40.0 38.0 41.0 33.0 41.0 26-27 37.789612970841034 40.0 38.0 41.0 33.0 41.0 28-29 37.6098561896139 40.0 37.0 41.0 33.0 41.0 30-31 37.599595979014474 40.0 37.0 41.0 33.0 41.0 32-33 37.46953874012428 40.0 37.0 41.0 33.0 41.0 34-35 37.27190798367792 40.0 37.0 41.0 32.0 41.0 36-37 37.35250753469681 40.0 37.0 41.0 32.0 41.0 38-39 37.33786293673336 40.0 37.0 41.0 32.0 41.0 40-41 37.20570187406204 40.0 37.0 41.0 31.5 41.0 42-43 36.99732409738673 40.0 37.0 41.0 31.0 41.0 44-45 36.790448609026754 40.0 37.0 41.0 31.0 41.0 46-47 36.51858093442644 40.0 36.0 41.0 30.0 41.0 48-49 36.234648442829325 39.0 36.0 41.0 30.0 41.0 50-51 35.326077182581514 38.5 35.0 40.0 28.0 40.5 52-53 35.447654011683426 38.5 35.0 40.0 28.5 40.5 54-55 35.80128244880747 39.0 35.0 40.5 28.5 41.0 56-57 35.577613578577896 39.0 35.0 41.0 28.0 41.0 58-59 35.35015720540265 39.0 35.0 40.0 27.5 41.0 60-61 35.06541543155519 38.0 35.0 40.0 26.5 41.0 62-63 34.74026845845684 38.0 34.0 40.0 26.0 41.0 64-65 34.39675915016062 37.5 34.0 40.0 26.0 41.0 66-67 33.98576314386 37.0 34.0 40.0 25.0 41.0 68-69 33.50244800128989 36.5 33.0 39.0 23.0 41.0 70-71 33.0724400635023 36.0 33.0 39.0 22.0 40.5 72-73 32.672380220025545 35.5 32.5 38.5 21.5 40.0 74-75 32.21888604313691 35.0 32.0 37.5 20.0 40.0 76-77 31.78547198829176 35.0 32.0 37.0 18.5 39.0 78-79 31.327115916504397 35.0 31.0 36.5 17.5 39.0 80-81 30.888715938829424 35.0 31.0 36.0 12.5 38.5 82-83 30.465738834385505 34.0 31.0 36.0 7.5 37.0 84-85 30.02211883116078 34.0 30.0 35.0 6.5 37.0 86-87 29.6287006833939 34.0 30.0 35.0 2.0 36.0 88-89 29.27288315328612 34.0 29.5 35.0 2.0 36.0 90-91 28.862139853895094 34.0 29.0 35.0 2.0 36.0 92-93 28.45558559787664 34.0 29.0 35.0 2.0 35.0 94-95 27.97800829746859 34.0 28.5 35.0 2.0 35.0 96-97 27.40118849764967 34.0 27.0 35.0 2.0 35.0 98-99 26.732907400746647 33.0 25.5 35.0 2.0 35.0 100-101 24.958039800563085 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.054012918712388114 1101 2 0.11574071285940235 1101 3 0.17879397689112153 1101 4 0.22419942675465876 1101 5 0.17814371126443262 1101 6 0.06401934088412986 1101 7 0.0931246045204972 1101 8 -0.14390094591489344 1101 9 0.011321296255538016 1101 10-11 0.08905748344606224 1101 12-13 0.19890219780658214 1101 14-15 0.2233692905566187 1101 16-17 0.10940269773222866 1101 18-19 0.1522911625399459 1101 20-21 0.16814440653208607 1101 22-23 0.16644889211349323 1101 24-25 0.30151198379031285 1101 26-27 0.44049792349674277 1101 28-29 0.2348682387722718 1101 30-31 0.3106988721599748 1101 32-33 0.28797416490056804 1101 34-35 0.20676719395054732 1101 36-37 0.37931162277484276 1101 38-39 0.3964172185173993 1101 40-41 0.32207969022289973 1101 42-43 0.6190966316167987 1101 44-45 0.5267024070023325 1101 46-47 0.5869358146521435 1101 48-49 0.6887699947699488 1101 50-51 0.6004007542832213 1101 52-53 0.7705170712875145 1101 54-55 0.9246686924719185 1101 56-57 1.050770537624544 1101 58-59 0.9985053848818453 1101 60-61 0.8222419461403234 1101 62-63 1.0091040440988905 1101 64-65 1.0345653891191375 1101 66-67 1.2162339265197843 1101 68-69 1.0984370702427313 1101 70-71 1.2775251105646177 1101 72-73 1.2766397695580665 1101 74-75 1.0799681247233508 1101 76-77 1.299445082634719 1101 78-79 1.3136654961012972 1101 80-81 1.1973371576081533 1101 82-83 0.920179581797651 1101 84-85 1.0062677029438944 1101 86-87 1.1304572480466106 1101 88-89 1.1869711836839443 1101 90-91 1.1780875153044263 1101 92-93 1.2864389308517339 1101 94-95 1.1419798116851325 1101 96-97 1.17887701132614 1101 98-99 1.1814406239645763 1101 100-101 1.4875720798785679 1102 1 0.11229351743634908 1102 2 0.016886633390985395 1102 3 0.3151593876138463 1102 4 0.12624967896994832 1102 5 0.10100443551050375 1102 6 0.218128736825264 1102 7 0.2383316465968548 1102 8 0.0609204214693122 1102 9 0.3910061464227397 1102 10-11 0.10014693204228564 1102 12-13 0.4206011702949368 1102 14-15 0.39144594838960245 1102 16-17 0.3866898761740032 1102 18-19 0.4411720032864892 1102 20-21 0.4341252702664349 1102 22-23 0.6082940757340296 1102 24-25 0.4640303631932383 1102 26-27 0.3639875594652864 1102 28-29 0.40674439087349157 1102 30-31 0.30063736893083615 1102 32-33 0.27567460340384997 1102 34-35 0.38345668938985966 1102 36-37 0.40533664044942697 1102 38-39 0.6774442530468221 1102 40-41 0.47916557354025713 1102 42-43 0.5209104997708778 1102 44-45 0.623987460156215 1102 46-47 0.7377163157863578 1102 48-49 0.7706369517480525 1102 50-51 0.9398222422449862 1102 52-53 0.8195656796576856 1102 54-55 0.7427885066610287 1102 56-57 0.8845231454395304 1102 58-59 0.7650728714469821 1102 60-61 0.7013900526822994 1102 62-63 0.8358345272332173 1102 64-65 0.9067640211277066 1102 66-67 0.9294252414657649 1102 68-69 0.7916181491082668 1102 70-71 0.5945920397838549 1102 72-73 0.6581918369427058 1102 74-75 0.5495743392386316 1102 76-77 0.6749216459140825 1102 78-79 0.6948033492135472 1102 80-81 0.49544673571682196 1102 82-83 0.6338870380882895 1102 84-85 0.6974416859502632 1102 86-87 0.6165051667825416 1102 88-89 0.6112925011889416 1102 90-91 0.4108694304056826 1102 92-93 0.4378787049639925 1102 94-95 0.35208078454650504 1102 96-97 0.4709566272128818 1102 98-99 0.8998746771488229 1102 100-101 0.8854671774032568 1103 1 0.43413571794304673 1103 2 0.29386300482989824 1103 3 0.41687336310632617 1103 4 0.4259885756143831 1103 5 0.34017898698147064 1103 6 0.25534889090209845 1103 7 0.09149978681711701 1103 8 0.3556182137241066 1103 9 0.16159711896811046 1103 10-11 0.1169700022041198 1103 12-13 0.3307079383007334 1103 14-15 0.31141195324974547 1103 16-17 0.22805666175903383 1103 18-19 0.3042737043360688 1103 20-21 0.38516192814348216 1103 22-23 0.24221295273108012 1103 24-25 0.17565334028852675 1103 26-27 0.15974527623277623 1103 28-29 -0.1068665909785338 1103 30-31 0.012690132490114081 1103 32-33 0.21829326324563425 1103 34-35 0.2830094529491305 1103 36-37 0.3544796527031906 1103 38-39 0.3573211983960789 1103 40-41 0.36578596545381004 1103 42-43 0.3977330528963563 1103 44-45 0.41683038451379417 1103 46-47 0.28584916827588813 1103 48-49 0.42431308017987845 1103 50-51 0.05648179966187428 1103 52-53 0.039664821377343173 1103 54-55 0.35643955882999734 1103 56-57 0.28004948844143485 1103 58-59 -0.053276102235862766 1103 60-61 -0.4512584660085608 1103 62-63 -0.1836706689820815 1103 64-65 0.30694692273569046 1103 66-67 0.3463342689203941 1103 68-69 0.3672928815419496 1103 70-71 0.09700918470914388 1103 72-73 0.2257103209921354 1103 74-75 0.3958141924003087 1103 76-77 0.4033224524907837 1103 78-79 0.1292011311276191 1103 80-81 0.023815873817230937 1103 82-83 0.3796634974233797 1103 84-85 0.36349114047692055 1103 86-87 0.43268258780523894 1103 88-89 0.4143307360268551 1103 90-91 0.5709988187516828 1103 92-93 0.484092060012852 1103 94-95 0.5477047181590393 1103 96-97 0.5833667921474799 1103 98-99 1.0421501541148501 1103 100-101 1.1497632324159213 1104 1 0.00816131421995081 1104 2 0.11065848392903987 1104 3 0.10728722498615895 1104 4 -0.07627603005336425 1104 5 -0.004321553199702066 1104 6 0.1621572512444871 1104 7 0.041169445256407755 1104 8 0.1399404941196849 1104 9 0.30894192076317495 1104 10-11 0.1942470735911357 1104 12-13 0.3297626212559379 1104 14-15 0.47206910441737193 1104 16-17 0.20799724183049761 1104 18-19 0.0506378695484031 1104 20-21 0.16260728674067337 1104 22-23 0.11228899361864109 1104 24-25 0.20309869888571797 1104 26-27 0.29069765718515583 1104 28-29 0.1255567448479411 1104 30-31 0.08292926653815158 1104 32-33 0.31033701792027557 1104 34-35 0.1513506812380072 1104 36-37 0.19251879506170866 1104 38-39 0.3916434787916572 1104 40-41 0.5998402328850361 1104 42-43 0.5892376652124938 1104 44-45 0.7143143868407265 1104 46-47 1.0717971862265259 1104 48-49 0.9235651370235587 1104 50-51 0.7912684281003308 1104 52-53 0.9653951047646956 1104 54-55 1.2052823245575794 1104 56-57 1.1973290529723144 1104 58-59 0.9301392970826754 1104 60-61 0.8946340794913112 1104 62-63 0.9848658555753858 1104 64-65 0.6950930625179552 1104 66-67 0.7785051739316131 1104 68-69 0.903100660150777 1104 70-71 0.898700367290381 1104 72-73 0.767730183038168 1104 74-75 0.6516003637623982 1104 76-77 0.5716719912591657 1104 78-79 0.5569467057972766 1104 80-81 0.7857881583680495 1104 82-83 0.8093308704808067 1104 84-85 0.6053624527016339 1104 86-87 0.621169457247607 1104 88-89 0.6857250267274253 1104 90-91 0.5532923033357449 1104 92-93 0.4399166673426933 1104 94-95 0.33941813127297493 1104 96-97 0.8269988872193643 1104 98-99 0.7076325774506778 1104 100-101 1.1483088985008578 1105 1 0.23486035562420327 1105 2 0.1396186984116774 1105 3 0.09717011949003762 1105 4 0.025539990383187217 1105 5 -0.04241245539257932 1105 6 -0.011300522486443754 1105 7 -0.27090532015321145 1105 8 -0.003077438449807346 1105 9 -0.14620136274341888 1105 10-11 -0.13501412478000674 1105 12-13 0.0535340252572567 1105 14-15 0.2530527399992266 1105 16-17 0.2498820585844257 1105 18-19 0.10982925989161885 1105 20-21 -0.0415116398344324 1105 22-23 0.2852598816475762 1105 24-25 0.4253082747260706 1105 26-27 0.13127736733007822 1105 28-29 0.08775038624713716 1105 30-31 0.2988788833527778 1105 32-33 0.2229947815341049 1105 34-35 0.1268672859374007 1105 36-37 0.37544238562244203 1105 38-39 0.2788187829371438 1105 40-41 0.47491294958079067 1105 42-43 0.6450587947873174 1105 44-45 0.5816854569775671 1105 46-47 0.38268319174034815 1105 48-49 0.5352516585097575 1105 50-51 0.5340094049068682 1105 52-53 0.6547873196520158 1105 54-55 0.9200178887126711 1105 56-57 0.9385208479997544 1105 58-59 1.1858111993514413 1105 60-61 1.223085909422771 1105 62-63 1.4973162185265494 1105 64-65 1.1184117260483148 1105 66-67 0.9147003834821632 1105 68-69 1.0619961251582382 1105 70-71 1.1192659134876166 1105 72-73 0.8910829917995855 1105 74-75 0.1431399342912698 1105 76-77 0.4124666898958864 1105 78-79 0.8166148647370015 1105 80-81 0.6582620435894881 1105 82-83 0.760572745180454 1105 84-85 0.48197802798554434 1105 86-87 0.21895760505852024 1105 88-89 0.15962921773837735 1105 90-91 0.37685630667439085 1105 92-93 0.530287712186766 1105 94-95 0.06077407633709342 1105 96-97 -0.6555048434632873 1105 98-99 0.03714670345922855 1105 100-101 0.26691299087003273 1106 1 0.3753334091407332 1106 2 0.5413377523046456 1106 3 0.30107249730546215 1106 4 0.3606927603185639 1106 5 0.47527999708248103 1106 6 0.46187847243167823 1106 7 0.3669326872500136 1106 8 0.3833959915018852 1106 9 0.4018568592278484 1106 10-11 0.19904070927482564 1106 12-13 0.4539041864969775 1106 14-15 0.46464066897846124 1106 16-17 0.3342976430000064 1106 18-19 0.4521813522437199 1106 20-21 0.027297570279124272 1106 22-23 0.35963719515532233 1106 24-25 0.8275292421644309 1106 26-27 0.8264119428994405 1106 28-29 0.5100165259045824 1106 30-31 0.8167124990124819 1106 32-33 0.40416988662224895 1106 34-35 0.6268045467442249 1106 36-37 0.4513411245646566 1106 38-39 0.38834572942061385 1106 40-41 0.39735162201946395 1106 42-43 0.6969754771387855 1106 44-45 0.8971694898528995 1106 46-47 0.5171803371070567 1106 48-49 0.9697676256344252 1106 50-51 0.7695950752751415 1106 52-53 0.8507218199343356 1106 54-55 1.0110210884115318 1106 56-57 0.7657998491918008 1106 58-59 0.866547130087362 1106 60-61 1.1650835253334293 1106 62-63 1.0446843093728972 1106 64-65 1.0011208044095739 1106 66-67 1.2497433598294876 1106 68-69 1.0507344399835077 1106 70-71 0.5107898484898143 1106 72-73 0.7215616918435046 1106 74-75 0.4086208302069494 1106 76-77 0.6868722360405641 1106 78-79 1.1375885770150624 1106 80-81 0.4232567107580678 1106 82-83 0.33700476726464856 1106 84-85 0.9885813280671094 1106 86-87 0.31588965851231166 1106 88-89 0.928670562866678 1106 90-91 0.8706921809450456 1106 92-93 0.8696440080647996 1106 94-95 1.3271960601503814 1106 96-97 0.8396799234606114 1106 98-99 0.8473449593096589 1106 100-101 1.1861990188517098 1107 1 0.08884506304974238 1107 2 -0.038261456433637875 1107 3 0.08235743753079561 1107 4 -0.0030296100263171866 1107 5 0.10635422087193547 1107 6 0.21357710492501525 1107 7 -0.381024653422557 1107 8 -0.26561063101467397 1107 9 -0.1543915281463697 1107 10-11 -0.0018279584812574967 1107 12-13 0.2557447300593765 1107 14-15 0.2185324725391382 1107 16-17 0.14392179368376645 1107 18-19 0.1300647271469586 1107 20-21 0.1427830781487387 1107 22-23 0.37627949325457877 1107 24-25 0.15181753665140008 1107 26-27 0.07877712738383735 1107 28-29 0.36853549038381317 1107 30-31 0.09780392414364059 1107 32-33 0.41679137109805 1107 34-35 0.5928749002317986 1107 36-37 0.7452475492657911 1107 38-39 0.838741360279819 1107 40-41 0.6728526110966087 1107 42-43 0.2124996880272363 1107 44-45 0.6597479709744221 1107 46-47 0.13051567726359536 1107 48-49 -0.13883734555332694 1107 50-51 -0.3908074361207454 1107 52-53 -0.3724225853292751 1107 54-55 0.4097481891253807 1107 56-57 0.21509889451733955 1107 58-59 0.21782002937351308 1107 60-61 0.2093769802229133 1107 62-63 0.34699788983217417 1107 64-65 0.4189843977698544 1107 66-67 0.45661099550506634 1107 68-69 0.7467867320622972 1107 70-71 0.41596745234290466 1107 72-73 0.5138554907598234 1107 74-75 0.6988074647644424 1107 76-77 0.9268954578518702 1107 78-79 0.8243091412190715 1107 80-81 0.6499918961025237 1107 82-83 1.245472127718763 1107 84-85 1.229378610255118 1107 86-87 1.2173259705446497 1107 88-89 1.299165961507775 1107 90-91 1.1587931528208486 1107 92-93 1.0341748414698024 1107 94-95 1.073347259943965 1107 96-97 1.8655464410489806 1107 98-99 1.839926880699526 1107 100-101 2.080410767368093 1108 1 0.07418685694815963 1108 2 0.0645676604717913 1108 3 0.08936788392720985 1108 4 -0.0585867049860056 1108 5 -0.06563724467158494 1108 6 0.12149918644569624 1108 7 0.2512178581460631 1108 8 0.2955298827152717 1108 9 0.5447411715682264 1108 10-11 0.33035082175648967 1108 12-13 0.015796269309568345 1108 14-15 0.16449938766562866 1108 16-17 0.3837732719735456 1108 18-19 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12 886.0 13 1140.0 14 1249.0 15 1606.0 16 2043.0 17 2741.0 18 2393.0 19 1689.0 20 1709.0 21 1815.0 22 1934.0 23 2180.0 24 2375.0 25 2641.0 26 3014.0 27 3579.0 28 4193.0 29 5073.0 30 6357.0 31 8100.0 32 10209.0 33 14012.0 34 19842.0 35 29405.0 36 46330.0 37 69077.0 38 62563.0 39 9788.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.689880304679 18.886056272345716 15.187004508005597 23.237058914969687 2 14.757084202314314 19.71330620973031 41.03910958329319 24.490500004662195 3 18.96738218235393 23.689101906591322 30.426512000895173 26.917003910159575 4 12.561347452701463 16.113050262173914 38.11856415101963 33.20703813410499 5 13.548196609221302 36.38948889683144 35.65632235707293 14.40599213687433 6 31.777483674141454 35.80349320558466 18.02803511845956 14.390988001814328 7 27.892923386658296 31.930666152990554 21.91367134464042 18.262739115710723 8 25.624304860847896 34.3729611840511 20.775262677163397 19.227471277937607 9 25.02757195892943 16.854466657346567 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34-35 21.891896509679114 26.269281727965904 29.713626246293543 22.125195516061442 36-37 21.757918805065053 25.935406906664348 29.95802940116561 22.348644887104992 38-39 21.339699838144973 26.4752573059992 29.86613564134666 22.31890721450917 40-41 21.70593690149071 26.548532356198557 29.703147158920572 22.04238358339016 42-43 21.3887430610996 26.44004510479409 29.538203946980126 22.633007887126187 44-45 21.564600912129837 26.48920107864945 29.564377229063886 22.38182078015683 46-47 21.900880359350634 26.66208987493554 29.210285977000066 22.226743788713758 48-49 21.771492833133475 26.780532963858118 28.99940353776662 22.448570665241785 50-51 21.662107776208963 26.820255453194097 29.21324579021027 22.304390980386675 52-53 21.879004487355722 26.99170095478628 28.59170965301821 22.53758490483979 54-55 21.558935420281568 26.874066985943234 28.798270127860793 22.768727465914402 56-57 21.708220982392483 26.8098338655765 28.881388471240232 22.600556680790785 58-59 21.86605449583663 26.617160767843632 28.93408032257012 22.582704413749614 60-61 21.567710719860827 26.47665053859476 29.416826523559152 22.53881221798526 62-63 21.294649583190306 26.529195317317445 29.701360164891067 22.474794934601185 64-65 21.366630579807616 26.59373704018378 29.401056511399982 22.638575868608626 66-67 21.314279057123994 26.349578657139528 29.22465146984583 23.111490815890644 68-69 21.4991666809048 26.236115878728384 29.33423007683932 22.930487363527494 70-71 21.444992248928983 26.166776952446636 29.34285208346925 23.04537871515513 72-73 21.27748151070833 26.03894984240801 29.477235763517264 23.206332883366397 74-75 21.051871296432925 26.536761907720507 29.27827525415304 23.133091541693528 76-77 21.331703445287175 26.196515415334098 29.158738566060947 23.31304257331778 78-79 21.296388341793467 25.975762809799257 29.254711061130408 23.473137787276873 80-81 21.330508566715697 25.94493258303059 29.056628390457718 23.667930459796 82-83 21.291204523565415 25.594494671761893 29.709044023984838 23.405256780687857 84-85 20.91223982603293 25.600652376514443 29.382882882882882 24.10422491456974 86-87 21.241797072185765 25.396186852017237 29.614724498116722 23.747291577680272 88-89 20.832000149109536 24.947889323446542 29.71349223853798 24.50661828890594 90-91 20.59192644020937 24.974605097618937 30.15392261932498 24.279545842846716 92-93 20.448596969485155 25.23622016667055 30.186630629791 24.128552234053295 94-95 20.03199801177403 25.3977228599388 30.116186955373646 24.454092172913526 96-97 19.891124554441607 25.913753873154672 30.056612125400907 24.138509447002818 98-99 20.097354740607397 26.277082029290295 29.293090888758073 24.332472341344236 100-101 20.122422860551012 26.089488905525336 28.983091914996724 24.80499631892693 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 396.0 1 228.0 2 57.5 3 44.0 4 30.0 5 32.5 6 45.5 7 83.0 8 154.5 9 209.0 10 224.5 11 224.5 12 241.5 13 279.5 14 312.5 15 336.5 16 358.5 17 381.0 18 424.0 19 464.0 20 505.0 21 587.5 22 718.5 23 853.0 24 1040.5 25 1341.5 26 1709.5 27 2176.5 28 2669.0 29 3103.0 30 3649.0 31 4384.5 32 5230.0 33 5942.0 34 6610.5 35 7352.0 36 8018.5 37 8703.0 38 9356.0 39 9921.0 40 10310.0 41 10833.5 42 11348.5 43 11609.0 44 11998.0 45 12334.5 46 12610.0 47 13375.0 48 14220.5 49 13865.5 50 12677.0 51 11830.0 52 11289.0 53 10896.5 54 10487.0 55 9506.5 56 8311.5 57 7291.0 58 6417.0 59 5514.0 60 4445.5 61 3550.5 62 2767.5 63 1988.5 64 1362.0 65 975.0 66 695.5 67 487.5 68 373.0 69 298.0 70 215.0 71 133.5 72 93.5 73 62.5 74 37.0 75 26.5 76 21.5 77 12.5 78 8.5 79 8.0 80 6.5 81 4.5 82 2.0 83 0.5 84 0.5 85 1.0 86 0.5 87 1.0 88 2.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 1.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26603991218822476 2 0.23906383717613205 3 0.24247460528110928 4 0.24030411648703287 5 0.23348258027707838 6 0.19410371215597752 7 0.19999503888275638 8 0.19534399146687834 9 0.1931735026728019 10-11 0.20774678457588647 12-13 0.20836692423133688 14-15 0.19611916603619134 16-17 0.20371587681545883 18-19 0.18929762982623688 20-21 0.19379364232825233 22-23 0.19332853758666452 24-25 0.19007280439554988 26-27 0.19689434060550437 28-29 0.18759224577374825 30-31 0.18836742034306125 32-33 0.1920882582757637 34-35 0.18712714103216044 36-37 0.1910030138787255 38-39 0.1906929440510003 40-41 0.1905379091371377 42-43 0.1832512681855954 44-45 0.1934835725005271 46-47 0.184181477668771 48-49 0.18914259491237426 50-51 0.18898755999851166 52-53 0.18712714103216044 54-55 0.19673930569164177 56-57 0.18604189663512224 58-59 0.18402644275490843 60-61 0.18774728068761085 62-63 0.18557679189353443 64-65 0.18588686172125962 66-67 0.19239832810348892 68-69 0.18681707120443525 70-71 0.1913130837064507 72-73 0.19611916603619134 74-75 0.18774728068761085 76-77 0.18743721085988566 78-79 0.18790231560147347 80-81 0.18402644275490843 82-83 0.19782455008868 84-85 0.18852245525692388 86-87 0.1846465824103588 88-89 0.18511168715194662 90-91 0.1838714078410458 92-93 0.19084797896486289 94-95 0.19007280439554988 96-97 0.17999553499448076 98-99 0.1837163729271832 100-101 0.1832512681855954 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 322508.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.74861601575174 #Duplication Level Percentage of deduplicated Percentage of total 1 87.6045757161432 66.3592536714498 2 7.705129518032232 11.673057944261156 3 2.091123391622555 4.751991085007201 4 0.899768980452925 2.726250200128522 5 0.4546537965045082 1.721969792576186 6 0.31526637298760857 1.4328594858070278 7 0.201410776163195 1.0679611281514267 8 0.1420714778634064 0.8609374258772435 9 0.10743160069918055 0.7324015562387813 >10 0.44987717920990733 5.763244963836195 >50 0.019264084930024152 1.00158910495802 >100 0.008607357096347966 1.389060649585755 >500 8.197482948902822E-4 0.5194229921227018 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 934 0.2896052190953403 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 739 0.22914160268892553 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 448 0.13891128282089127 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 437 0.135500514715914 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 430 0.1333300259218376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 423 0.13115953712776118 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 392 0.12154737246827985 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 3.100698277252037E-4 12-13 0.0 0.0 0.0 0.0 6.201396554504074E-4 14-15 0.0 0.0 0.0 0.0 6.201396554504074E-4 16-17 0.0 3.100698277252037E-4 0.0 3.100698277252037E-4 6.201396554504074E-4 18-19 0.0 3.100698277252037E-4 0.0 4.651047415878055E-4 6.201396554504074E-4 20-21 0.0 3.100698277252037E-4 0.0 6.201396554504074E-4 6.201396554504074E-4 22-23 0.0 3.100698277252037E-4 0.0 0.001085244397038213 6.201396554504074E-4 24-25 0.0 3.100698277252037E-4 0.0 0.0017053840524886205 6.201396554504074E-4 26-27 0.0 3.100698277252037E-4 0.0 0.0026355935356642316 6.201396554504074E-4 28-29 0.0 3.100698277252037E-4 0.0 0.005271187071328463 6.201396554504074E-4 30-31 0.0 3.100698277252037E-4 0.0 0.01255782802287075 6.201396554504074E-4 32-33 0.0 3.100698277252037E-4 0.0 0.023100202165527675 6.201396554504074E-4 34-35 0.0 3.100698277252037E-4 0.0 0.0331774715665968 6.201396554504074E-4 36-37 0.0 3.100698277252037E-4 0.0 0.04666550907264316 6.201396554504074E-4 38-39 0.0 3.100698277252037E-4 0.0 0.06837039701340743 6.201396554504074E-4 40-41 0.0 3.100698277252037E-4 0.0 0.0993773797859278 6.201396554504074E-4 42-43 0.0 3.100698277252037E-4 0.0 0.13891128282089127 6.201396554504074E-4 44-45 0.0 3.100698277252037E-4 0.0 0.17317399878452627 6.201396554504074E-4 46-47 0.0 3.100698277252037E-4 0.0 0.2354980341572922 9.302094831756112E-4 48-49 0.0 3.100698277252037E-4 0.0 0.31363563074404355 9.302094831756112E-4 50-51 0.0 3.100698277252037E-4 0.0 0.352394359209694 9.302094831756112E-4 52-53 0.0 3.100698277252037E-4 0.0 0.4243305592419413 9.302094831756112E-4 54-55 0.0 3.100698277252037E-4 0.0 0.5542498170588016 9.302094831756112E-4 56-57 0.0 3.100698277252037E-4 0.0 0.7788954072457117 0.001085244397038213 58-59 0.0 3.100698277252037E-4 0.0 1.0519118905577536 0.0012402793109008148 60-61 0.0 3.100698277252037E-4 0.0 1.3787254889801184 0.0012402793109008148 62-63 0.0 3.100698277252037E-4 0.0 1.7322050925868506 0.0012402793109008148 64-65 0.0 3.100698277252037E-4 0.0 2.130024681558287 0.0012402793109008148 66-67 0.0 3.100698277252037E-4 0.0 2.620245079191834 0.0012402793109008148 68-69 0.0 3.100698277252037E-4 0.0 3.1549604971039478 0.0012402793109008148 70-71 0.0 3.100698277252037E-4 0.0 3.525648976149429 0.0012402793109008148 72-73 0.0 3.100698277252037E-4 0.0 4.026721817753359 0.0012402793109008148 74-75 0.0 3.100698277252037E-4 0.0 4.604071836977687 0.0012402793109008148 76-77 0.0 3.100698277252037E-4 0.0 5.270721966586875 0.0012402793109008148 78-79 0.0 3.100698277252037E-4 0.0 5.978921453111241 0.0012402793109008148 80-81 0.0 3.100698277252037E-4 0.0 7.054553685489973 0.0012402793109008148 82-83 0.0 3.100698277252037E-4 0.0 8.136852419164796 0.0012402793109008148 84-85 0.0 3.100698277252037E-4 0.0 9.67262827588773 0.0012402793109008148 86-87 0.0 3.100698277252037E-4 0.0 11.274604040829995 0.0012402793109008148 88-89 0.0 3.100698277252037E-4 0.0 13.195486623587632 0.0012402793109008148 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGT 75 3.0664937E-4 31.679096 1 CGACAGC 80 4.480308E-4 29.69454 8 GGACGAT 70 0.008373065 27.149292 7 GCGTGCC 90 8.921144E-4 26.395145 9 TACATGG 885 0.0 25.768822 2 GTACATG 945 0.0 25.644983 1 CATGGGG 450 0.0 25.343277 4 GTGCTAT 95 0.0012218375 25.009811 1 GTATCAA 1520 0.0 25.009811 1 GTTACAC 115 1.276896E-4 24.788486 3 ACATGGG 940 0.0 24.76651 3 GGTATCA 1125 0.0 24.4985 1 CTCTATA 100 0.0016487915 23.755632 2 CATGGGA 395 0.0 22.85707 4 GTCTAGA 170 3.1266482E-6 22.361717 1 GAGTACT 990 0.0 22.074226 12-13 CGGTGGT 55 0.002875939 21.590996 94-95 ATCAACG 1780 0.0 21.353376 3 TATCAAC 1795 0.0 21.174936 2 CAACGCA 1780 0.0 21.089735 5 >>END_MODULE