FastQCFastQC Report
Thu 26 May 2016
SRR937289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322508
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10990.3407667406699989No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10160.31503094496880696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.2964267553052948No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.1739491733538393No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC4880.15131407592989943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT2000.059.374814
TAAGGTG2400.049.4790045
GGTAAGG2400.047.4998443
AGGTAAG2500.043.699862
AAGGTAA2700.040.475381
GGTGTGC3500.035.28568
TACACGA550.00257692834.545345
CATATCC702.0501086E-433.9284633
GTGTGCA3650.033.8355069
GAACCGC600.003946838431.6665636
AAGGTGT3950.031.2657186
AGGTGTG4150.030.9035157
GTACTGG953.4758516E-530.0092071
GTCTACC804.4784954E-429.6966111
GTACATG11050.026.6597631
GTATAGA1257.3517986E-626.6081661
ACATGGG10000.025.174923
TACATGG10800.025.0693632
GTGCACT2800.023.74992410-11
GTTATAG1100.00286781621.5975361
Produced by FastQC (version 0.11.5)