FastQCFastQC Report
Thu 26 May 2016
SRR937288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591253
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14460.24456535527092463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10500.17758895092287058No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7920.1339528086961081No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7740.13090842668028746No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7320.12380486864337263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6330.10706076755635913No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGCG600.003944561431.6746085
AGGCGAC700.00837806527.1496645
CAGTGCG1056.896421E-527.145069
TACATGG17350.026.0085532
GTACATG18300.025.9627931
ACATGGG17850.024.4837823
GTATCAA26250.024.4346961
ATAGCGA1050.002192847722.62286
GTATAGG1703.1378422E-622.3585471
CTACACG1302.9358626E-421.9267164
GGTATCA19800.021.8362831
ATCAACG30000.021.3767363
CAACGCA30150.021.1164055
TCAACGC30250.021.0448134
GAGTACT14250.020.99814612-13
AACGCAG30750.020.8571196
TATCAAC30900.020.7523522
TGAACCG1404.8396975E-420.3622475
CCTACAC3052.0736479E-1020.2475453
TAAGGTG2401.4065517E-719.7966295
Produced by FastQC (version 0.11.5)