FastQCFastQC Report
Thu 26 May 2016
SRR937288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591253
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.33048458105075157No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15930.26942780840012653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.2641847060395465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.16101398217006932No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC6080.10283245920105268No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTA250.00501731557.007917
TAAGGTG2800.032.2311675
GTAAGGT2700.029.9065044
GGTATCA27400.027.9215471
ACGACCC908.9351356E-426.3925558
GTGTAGG2351.7462298E-1024.2648451
GGTAAGG3450.023.405093
GGACCAT2853.6379788E-1223.3326266
AACGACC1050.00219319822.6221897
GAGTACT20400.022.58892312-13
GTACTTT20200.022.57357414-15
GTATCAA36000.022.4393651
AGGTAAG3400.022.3522642
GGTGTGC5050.021.6366678
TCAACGC40400.019.7518774
ATCAACG40500.019.7031063
GTACATG18850.019.6628911
GTCCTAT1456.124977E-419.6628911
TAAGGGT1707.9473706E-519.5582314
TATCAAC41450.019.4807132
Produced by FastQC (version 0.11.5)