##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937288_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 591253 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.432554253424506 33.0 31.0 34.0 31.0 34.0 2 32.64891002667217 34.0 31.0 34.0 31.0 34.0 3 32.713983692260335 34.0 31.0 34.0 31.0 34.0 4 36.283768539017984 37.0 35.0 37.0 35.0 37.0 5 36.21966231038151 37.0 35.0 37.0 35.0 37.0 6 36.26796312238585 37.0 37.0 37.0 35.0 37.0 7 36.223094005442675 37.0 36.0 37.0 35.0 37.0 8 36.19137661880785 37.0 36.0 37.0 35.0 37.0 9 37.954060275381266 39.0 38.0 39.0 35.0 39.0 10-11 37.86807762497611 39.0 38.0 39.0 35.0 39.0 12-13 37.790942286973596 39.0 37.5 39.0 35.0 39.0 14-15 39.075326467688114 40.0 38.0 41.0 36.0 41.0 16-17 39.03306790832351 40.0 38.0 41.0 36.0 41.0 18-19 39.00263761875204 40.0 38.0 41.0 36.0 41.0 20-21 38.881221744329416 40.0 38.0 41.0 35.0 41.0 22-23 38.73203180364413 40.0 38.0 41.0 35.0 41.0 24-25 38.669227048319414 40.0 38.0 41.0 34.5 41.0 26-27 38.62867926251537 40.0 38.0 41.0 34.5 41.0 28-29 38.49923636751103 40.0 38.0 41.0 34.0 41.0 30-31 38.64916457083516 40.0 38.0 41.0 35.0 41.0 32-33 38.63172449019286 40.0 38.0 41.0 35.0 41.0 34-35 38.507825753104 40.0 38.0 41.0 35.0 41.0 36-37 38.36701039994723 40.0 38.0 41.0 34.0 41.0 38-39 38.25650440674296 40.0 38.0 41.0 34.0 41.0 40-41 38.09975086807171 40.0 38.0 41.0 34.0 41.0 42-43 37.920985601764386 40.0 38.0 41.0 33.0 41.0 44-45 37.66260551743501 40.0 37.0 41.0 33.0 41.0 46-47 37.36387553213261 40.0 37.0 41.0 32.5 41.0 48-49 37.06287240825839 40.0 37.0 41.0 32.0 41.0 50-51 36.84353567762024 40.0 36.5 41.0 31.0 41.0 52-53 36.60861340238443 39.0 36.0 41.0 31.0 41.0 54-55 36.38560480877052 39.0 36.0 41.0 30.5 41.0 56-57 36.103032035355426 39.0 35.0 40.0 30.0 41.0 58-59 35.81739712102941 38.5 35.0 40.0 29.0 41.0 60-61 35.47387243701089 38.0 35.0 40.0 28.5 41.0 62-63 35.09785235762017 38.0 34.0 40.0 28.0 41.0 64-65 34.722872442084864 37.0 34.0 40.0 28.0 41.0 66-67 34.29766783424355 36.5 34.0 39.0 26.5 41.0 68-69 33.850830355194816 36.0 33.0 39.0 26.0 40.0 70-71 33.43524768584683 35.5 33.0 39.0 26.0 40.0 72-73 32.95745307000556 35.0 32.5 38.0 25.5 40.0 74-75 32.47509780077226 35.0 32.0 37.0 24.5 39.0 76-77 31.22305425934414 34.0 30.5 36.0 23.0 38.5 78-79 31.82582329391986 34.5 32.0 36.0 24.0 39.0 80-81 31.626210776097544 35.0 32.0 36.0 23.5 37.5 82-83 31.29118245488818 34.0 31.0 35.5 22.5 37.0 84-85 30.933763549614124 34.0 31.0 35.0 20.5 36.5 86-87 30.561270725053404 34.0 31.0 35.0 19.5 36.0 88-89 30.131487704924965 34.0 30.5 35.0 17.5 36.0 90-91 29.660241893064388 34.0 30.0 35.0 12.0 35.0 92-93 29.310598001194073 34.0 29.5 35.0 6.0 35.0 94-95 28.887771393971786 34.0 29.0 35.0 2.0 35.0 96-97 28.430783437885303 34.0 29.0 35.0 2.0 35.0 98-99 27.683430781746562 33.5 28.0 35.0 2.0 35.0 100-101 25.197483141734587 31.0 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.14061524766470512 1101 2 -0.10797204750608103 1101 3 -0.0967494912330622 1101 4 -0.06240486580523452 1101 5 -0.0659026211717233 1101 6 -0.09730687281002304 1101 7 -0.11645341067963955 1101 8 -0.03216818306157876 1101 9 -0.24914773019604297 1101 10-11 -0.11447126821460785 1101 12-13 -0.2208698284493451 1101 14-15 -0.28096894390426286 1101 16-17 -0.22988436375494103 1101 18-19 -0.18634622369613396 1101 20-21 -0.1794211849058911 1101 22-23 -0.27059101634014127 1101 24-25 -0.26497011568044115 1101 26-27 -0.23323392813859556 1101 28-29 -0.3373525817144909 1101 30-31 -0.36504202044510237 1101 32-33 -0.14988821204671154 1101 34-35 -0.3019616659738844 1101 36-37 -0.28246973389396146 1101 38-39 -0.1505902261244998 1101 40-41 -0.1691469648170525 1101 42-43 -0.19662325515488988 1101 44-45 -0.11620209749382582 1101 46-47 -0.1502774944296661 1101 48-49 -0.07955038459338937 1101 50-51 0.05293935358536572 1101 52-53 0.04295136321488968 1101 54-55 0.005508942175758591 1101 56-57 -0.09416959494264887 1101 58-59 -0.0498916947636161 1101 60-61 0.07645916408221609 1101 62-63 -0.09117642117534785 1101 64-65 -0.08281361647858887 1101 66-67 -0.06132937544577288 1101 68-69 0.15050220663022174 1101 70-71 0.025402062755695454 1101 72-73 0.21430748160318558 1101 74-75 0.004686100915876068 1101 76-77 -0.07416925342690206 1101 78-79 -0.21301414682693576 1101 80-81 -0.03997104663555362 1101 82-83 0.05786687042983729 1101 84-85 -0.050563785738219735 1101 86-87 -0.16005484305010853 1101 88-89 -0.12800226078303467 1101 90-91 -0.13526250884898872 1101 92-93 -0.07800187757889177 1101 94-95 -0.3229607500734204 1101 96-97 -0.2982632216240937 1101 98-99 -0.32280093434959767 1101 100-101 0.3462964389864247 1102 1 -0.09193397715142737 1102 2 -0.12151214393851006 1102 3 -0.009309939683056712 1102 4 -0.023316946942465222 1102 5 -0.024298763874611495 1102 6 -0.05448068281746998 1102 7 -0.024333002000005877 1102 8 -0.12671365704513704 1102 9 -0.16303385725449715 1102 10-11 -0.14676419329595092 1102 12-13 -0.3159115409980302 1102 14-15 -0.21118639795988514 1102 16-17 -0.21402967523595606 1102 18-19 -0.353330946319268 1102 20-21 -0.07998103466231754 1102 22-23 -0.1610514758374606 1102 24-25 -0.23618204251153685 1102 26-27 -0.28872355612895007 1102 28-29 -0.31299947367524794 1102 30-31 -0.2807162099815059 1102 32-33 -0.20339199486349457 1102 34-35 -0.22795351946501086 1102 36-37 -0.6304986778769148 1102 38-39 -0.49144642919565484 1102 40-41 -0.3252108753551326 1102 42-43 -0.31271189743905126 1102 44-45 -0.27164058707549543 1102 46-47 -0.24898353232553916 1102 48-49 -0.06825806176927074 1102 50-51 0.10731088570634739 1102 52-53 0.013973525567742229 1102 54-55 0.1816166143625182 1102 56-57 0.07646608933202259 1102 58-59 0.10980535916513645 1102 60-61 0.0614472793022145 1102 62-63 -0.029311419446983678 1102 64-65 0.03703111519622837 1102 66-67 0.014015140628082179 1102 68-69 0.18019455653810468 1102 70-71 0.0456338070565252 1102 72-73 0.10094146957942485 1102 74-75 0.02963790252023557 1102 76-77 0.013145570297044884 1102 78-79 0.1996977568404219 1102 80-81 0.2060278628868808 1102 82-83 0.17209172262736772 1102 84-85 0.20806025252115035 1102 86-87 0.3679251719884995 1102 88-89 0.2141083478531165 1102 90-91 0.0902480734161415 1102 92-93 0.09517469069533746 1102 94-95 0.2592161781296838 1102 96-97 0.1974817776809914 1102 98-99 0.1857892492141282 1102 100-101 0.7258931048334496 1103 1 -0.08174183011718128 1103 2 -0.12284881895939748 1103 3 -0.10298858073011985 1103 4 -0.17023980965480234 1103 5 -0.1114332667985849 1103 6 -0.19502649178433273 1103 7 -0.06123080205569664 1103 8 -0.15444966372851354 1103 9 -0.14334574809270606 1103 10-11 -0.09209975442098539 1103 12-13 -0.14133621379112782 1103 14-15 -0.2014119618696597 1103 16-17 -0.19393777882827834 1103 18-19 -0.20015634314466269 1103 20-21 -0.24375157211706266 1103 22-23 -0.16476910323929417 1103 24-25 -0.20644102329683278 1103 26-27 -0.2488460846725289 1103 28-29 -0.3111336981252677 1103 30-31 -0.2088977750051697 1103 32-33 -0.2690700789626348 1103 34-35 -0.2964998853797951 1103 36-37 -0.133394807088834 1103 38-39 -0.19995380875567292 1103 40-41 -0.41940469323316876 1103 42-43 -0.35324378270777146 1103 44-45 -0.6105712470587861 1103 46-47 -0.3813004356950742 1103 48-49 -0.2683416039580706 1103 50-51 -0.10534575589766604 1103 52-53 -0.1565778528783639 1103 54-55 -0.30512802501369407 1103 56-57 -0.47591486304893493 1103 58-59 -0.43667194520690344 1103 60-61 -0.44593228070891655 1103 62-63 -0.46888256954444785 1103 64-65 -0.5012869020691681 1103 66-67 -0.037906329714438414 1103 68-69 -0.0028324554362768595 1103 70-71 -0.02029490360903452 1103 72-73 0.14013667982726474 1103 74-75 -0.02317468450287663 1103 76-77 0.04011577358303953 1103 78-79 0.2270299762779011 1103 80-81 0.07996269997961392 1103 82-83 0.20149857308684815 1103 84-85 0.04768709741114918 1103 86-87 -0.10610713099116609 1103 88-89 -0.033677784143542056 1103 90-91 -0.17502622343710428 1103 92-93 -4.950391849192215E-4 1103 94-95 0.11474388628897003 1103 96-97 0.20704735829920295 1103 98-99 0.4269337772007624 1103 100-101 0.8003431187571515 1104 1 0.04905080031386433 1104 2 0.1005225866538737 1104 3 0.034301208628775726 1104 4 0.09104699514345072 1104 5 0.09507537120864384 1104 6 0.04257897225745211 1104 7 -0.017254448959221236 1104 8 -0.15056973489880932 1104 9 0.04644935034040287 1104 10-11 0.0421365998234009 1104 12-13 0.042587335540218874 1104 14-15 0.10018363016386189 1104 16-17 -0.031227568187169652 1104 18-19 -0.037463351122625 1104 20-21 -0.10633551288501764 1104 22-23 -0.09521736128899505 1104 24-25 -0.019504215628806776 1104 26-27 0.029332185430213542 1104 28-29 -0.1491064927071406 1104 30-31 -0.050515177289511826 1104 32-33 -0.03954588680284843 1104 34-35 0.097685960915328 1104 36-37 -0.14907522951895658 1104 38-39 -0.19664783389485052 1104 40-41 0.07739838316010861 1104 42-43 0.14490234597270302 1104 44-45 0.27164765898494636 1104 46-47 0.20056261888795035 1104 48-49 0.3067028774745211 1104 50-51 0.5653596335301003 1104 52-53 0.5836333621821908 1104 54-55 0.6520033530497855 1104 56-57 0.5059022752165774 1104 58-59 0.5505862766836955 1104 60-61 0.5400747215294999 1104 62-63 0.6544199615925521 1104 64-65 0.6002857318055987 1104 66-67 0.5885185110133406 1104 68-69 0.7512397479730097 1104 70-71 0.6547725359823175 1104 72-73 0.8291225031642284 1104 74-75 1.1145455703326803 1104 76-77 0.927712628315291 1104 78-79 0.76510034303449 1104 80-81 0.8489232693419559 1104 82-83 0.9224823301895277 1104 84-85 1.103710744314668 1104 86-87 0.9575156664604769 1104 88-89 1.0530351814766163 1104 90-91 0.81118166269043 1104 92-93 0.7961932464145747 1104 94-95 1.188560106228131 1104 96-97 1.1581464801496288 1104 98-99 1.1391016013412667 1104 100-101 1.5363874280035468 1105 1 0.04280197721899981 1105 2 -0.016104729133310514 1105 3 -0.025640561726248734 1105 4 -0.20103406283994474 1105 5 -0.029020403520846116 1105 6 -0.017197669253619097 1105 7 -0.10316200577657497 1105 8 -0.15165900586944758 1105 9 0.013690105297889943 1105 10-11 -0.09236084357650753 1105 12-13 -0.11631730979489419 1105 14-15 -0.10687200666856 1105 16-17 -0.0976615094070965 1105 18-19 -0.010057757020909719 1105 20-21 -0.176002503049709 1105 22-23 -0.17999074367815382 1105 24-25 -0.24700685913881415 1105 26-27 -0.15405788076618165 1105 28-29 -0.03378282568800017 1105 30-31 -0.22157272614012413 1105 32-33 -0.02169024417641907 1105 34-35 -0.015388187729605818 1105 36-37 -0.22606148326694608 1105 38-39 -0.2908784638469015 1105 40-41 -0.34596445126201303 1105 42-43 -0.285420524372185 1105 44-45 -0.5358526669272408 1105 46-47 -0.4090800364997449 1105 48-49 -0.18422952109436608 1105 50-51 -0.10874990193638467 1105 52-53 -0.15454906173542327 1105 54-55 -0.10207199784901633 1105 56-57 -0.01814425055945179 1105 58-59 -0.004742025531051297 1105 60-61 -0.0665825781793501 1105 62-63 0.013340540412606572 1105 64-65 -0.2589433772228915 1105 66-67 -0.12509013362649313 1105 68-69 -0.14143084895327718 1105 70-71 0.019239416061118675 1105 72-73 0.02655576358979772 1105 74-75 0.2541562139006217 1105 76-77 0.39208535598306327 1105 78-79 0.10827863873100085 1105 80-81 0.0282256724011809 1105 82-83 0.1553357276430667 1105 84-85 -0.2826777140482406 1105 86-87 -0.0736849940549007 1105 88-89 0.3097728728594973 1105 90-91 0.291639967657332 1105 92-93 0.5028548916193216 1105 94-95 0.5996107022853181 1105 96-97 0.6146089567925905 1105 98-99 0.51438152370174 1105 100-101 0.8688526278239372 1106 1 -0.10527165235258451 1106 2 0.074862396434078 1106 3 0.037708870554709506 1106 4 -0.03394547753680399 1106 5 -0.20526645766437923 1106 6 -0.10969250717082701 1106 7 -0.12335246351793927 1106 8 -0.09953627817646549 1106 9 -0.3090314239994072 1106 10-11 -0.26857167668604376 1106 12-13 -0.26640294093380845 1106 14-15 -0.49687451616723166 1106 16-17 -0.16178140180683442 1106 18-19 -0.01594407232840922 1106 20-21 -0.23684683928730976 1106 22-23 -0.27168430307182945 1106 24-25 -0.22992025501883262 1106 26-27 -0.30652973695870145 1106 28-29 -0.3304121727340572 1106 30-31 -0.2666006167964241 1106 32-33 -0.17622253085803408 1106 34-35 -0.10859625158533248 1106 36-37 -0.04669836761427604 1106 38-39 -0.389581009006875 1106 40-41 -0.19480898121530288 1106 42-43 -0.3252026822768883 1106 44-45 -0.5078585488399199 1106 46-47 -0.5520312069387288 1106 48-49 -0.5729662746836084 1106 50-51 -0.5055742854582235 1106 52-53 -0.35004120964615737 1106 54-55 -0.45837017912287337 1106 56-57 -0.5510627094631104 1106 58-59 -0.39350079226655765 1106 60-61 -0.28530740064332605 1106 62-63 -0.09728652607149968 1106 64-65 -0.03816913084295237 1106 66-67 -0.24529952267363342 1106 68-69 0.14650550663828454 1106 70-71 0.25339105141774354 1106 72-73 0.0015884531119851886 1106 74-75 -0.09454743191666637 1106 76-77 -0.5127914364373751 1106 78-79 -0.38108020637129414 1106 80-81 -0.14820749348548645 1106 82-83 -0.0488075867442852 1106 84-85 -0.10156015063936863 1106 86-87 0.10072956632055607 1106 88-89 0.09427958026515881 1106 90-91 0.16234795446705164 1106 92-93 0.5211786345586056 1106 94-95 0.16296013456627634 1106 96-97 0.3112149699036202 1106 98-99 -0.0526573118908793 1106 100-101 0.5935011879440388 1107 1 -0.036445382892246414 1107 2 -0.18293797314256466 1107 3 -0.0796937023127029 1107 4 -0.08859677075985672 1107 5 -0.17159129545802188 1107 6 0.12603953205389473 1107 7 -0.10682187898466111 1107 8 -0.12716006568761173 1107 9 0.06578777273225711 1107 10-11 -0.0029015681155257766 1107 12-13 -0.0851480334165231 1107 14-15 -0.161902883831047 1107 16-17 -0.04293240279276489 1107 18-19 0.0783127303023079 1107 20-21 0.1381937492014984 1107 22-23 -0.022698277319534554 1107 24-25 0.07871461388773326 1107 26-27 -0.17244898505325068 1107 28-29 0.08855254037174376 1107 30-31 0.12200390667995009 1107 32-33 -0.2092659885840149 1107 34-35 -0.2374285429014691 1107 36-37 0.028787365900889483 1107 38-39 0.11607481241558304 1107 40-41 0.10335258163339489 1107 42-43 -0.1306701731112625 1107 44-45 -0.10519712473006138 1107 46-47 0.038730557819242506 1107 48-49 0.22400567645670577 1107 50-51 -0.002070391915189873 1107 52-53 0.024854736227986507 1107 54-55 0.06701607569609536 1107 56-57 0.17791245333362582 1107 58-59 0.09307991536054061 1107 60-61 0.2125938054989689 1107 62-63 -0.030821768687346207 1107 64-65 0.05982771653842889 1107 66-67 0.5132004448555776 1107 68-69 0.39831353932161306 1107 70-71 0.39090485150394017 1107 72-73 0.22174336830418184 1107 74-75 0.5081842471774607 1107 76-77 0.32364138149595334 1107 78-79 0.5182810046120991 1107 80-81 0.5359461701053085 1107 82-83 0.41612100703430954 1107 84-85 0.5365351717955846 1107 86-87 0.5007726639159493 1107 88-89 0.7441293290593123 1107 90-91 0.8419704667616728 1107 92-93 1.21400495124918 1107 94-95 0.9259646863808015 1107 96-97 0.7311744699715135 1107 98-99 0.805259362155514 1107 100-101 1.6422398453063494 1108 1 0.04569115913561461 1108 2 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1.2337720280475963 2316 86-87 1.0188531570528845 2316 88-89 1.3091581790037665 2316 90-91 1.6093431507024718 2316 92-93 1.7847321132030913 2316 94-95 2.2185138746401307 2316 96-97 1.7678013559694357 2316 98-99 1.6764201692371898 2316 100-101 1.6352155110053772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 4.0 5 6.0 6 21.0 7 42.0 8 105.0 9 214.0 10 341.0 11 556.0 12 885.0 13 1232.0 14 1582.0 15 2337.0 16 3140.0 17 4621.0 18 4378.0 19 2763.0 20 2421.0 21 2530.0 22 2574.0 23 2848.0 24 3306.0 25 3709.0 26 4458.0 27 5650.0 28 7067.0 29 8851.0 30 11293.0 31 14427.0 32 19099.0 33 26037.0 34 37641.0 35 55790.0 36 90671.0 37 141333.0 38 117404.0 39 11912.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98873567420104 19.694800403457823 15.063666456814442 22.252797465526694 2 15.93852377916053 20.783319492670653 39.37789744830047 23.900259279868347 3 20.157529856085297 23.989392019998206 29.81583180127627 26.03724632264022 4 13.478324845709027 17.01082277806624 36.70239305339677 32.80845932282796 5 14.010266471598069 37.39076017556174 34.17365051712911 14.425322835711086 6 32.786302987046156 36.211571019512796 16.821563696082727 14.180562297358323 7 28.888648345124672 32.8324761142861 20.321419087936974 17.95745645265225 8 26.591492981853794 35.1698849730995 19.28210089420265 18.956521150844054 9 26.336441421861707 17.17082196623104 19.986706198530914 36.50603041337634 10-11 25.191161820743403 26.82861651441938 27.118932166094716 20.8612894987425 12-13 26.032945323414893 23.86455510789517 27.600630558402766 22.501869010287166 14-15 22.968847515361443 25.661011445185057 25.744055421283274 25.626085618170226 16-17 22.41189473880048 28.87494862605348 26.534072554388732 22.17908408075731 18-19 22.318787388816634 27.65059965869095 28.767042196826065 21.263570755666354 20-21 22.98770577062611 26.466081356035403 28.939557177722563 21.60665569561592 22-23 22.731370370182443 26.160467079014087 29.00892855632737 22.0992339944761 24-25 22.820784083903522 26.428392026729785 28.967417363706243 21.783406525660443 26-27 22.472858396856836 26.637417949398817 28.936695244490075 21.953028409254276 28-29 22.17183778956484 27.287849778090496 28.80299104243343 21.737321389911234 30-31 22.508545440092277 26.82532460833047 28.634623873786257 22.03150607779099 32-33 22.53679896761623 26.671915406771717 28.6861123749055 22.10517325070655 34-35 22.798192990141274 26.770773256118787 28.648395864376162 21.782637889363777 36-37 22.984576822443184 26.400288878026835 28.7358372811639 21.87929701836608 38-39 22.498942917547566 26.887019027484143 28.723044397463006 21.890993657505287 40-41 22.6700098519668 26.991429211715808 28.514044338078907 21.824516598238485 42-43 22.587032108062665 27.01811898497147 28.173867151125176 22.220981755840693 44-45 22.86263240947615 26.89119547807791 28.135248362376174 22.110923750069766 46-47 23.166140383220043 27.189206650959914 27.732882539285214 21.911770426534833 48-49 22.959883501648196 27.397070289706775 27.63656167495133 22.006484533693698 50-51 22.682252775038776 27.465061488060105 27.874108038352446 21.978577698548676 52-53 22.9392493568743 27.585399143852126 27.372799799747316 22.10255169952626 54-55 22.83743169167852 27.61880277985905 27.31800092346254 22.22576460499989 56-57 22.941617209553268 27.56493413141244 27.445019306455947 22.04842935257834 58-59 23.07683851075597 27.186331401278302 27.48214385381554 22.254686234150185 60-61 22.65561443240034 27.538718619609543 27.776603247679084 22.029063700311035 62-63 22.439547875444184 27.377873769773686 28.1342335683709 22.04834478641123 64-65 22.41468542231498 27.721719805227202 27.72087414355614 22.14272062890167 66-67 22.489357347869692 27.44476560795463 27.502693432422333 22.56318361175334 68-69 22.482422922166993 27.297705043357073 27.701085660453305 22.518786374022625 70-71 22.65705205724115 27.346330589443095 27.61212205265766 22.384495300658095 72-73 22.607749981818273 27.136183664184365 27.71191012984289 22.544156224154467 74-75 22.412233003468902 27.773051468660626 27.1546190886135 22.660096439256968 76-77 22.552020877695337 27.33905789907197 27.378550299110533 22.730370924122163 78-79 22.68081515019797 27.340833788581197 27.229037315666897 22.749313745553934 80-81 22.601849463638633 27.311005027462887 27.102802947979075 22.984342560919405 82-83 22.605354912418825 27.375998200427397 27.345300603888532 22.673346283265243 84-85 22.395809548937926 27.303048292682103 27.40444381301279 22.89669834536718 86-87 22.32563724835223 27.18979861412965 27.28028441293321 23.204279724584907 88-89 21.98635778592244 27.272081494723917 27.206289016715345 23.535271702638294 90-91 22.06759073428954 27.070884934720702 27.387449561520356 23.474074769469404 92-93 21.974622483221477 27.11735832972505 27.475170491448363 23.43284869560511 94-95 21.749910782693703 27.476562486786538 27.26827601720414 23.505250713315622 96-97 21.583399999661733 27.57635056397177 27.40282078906999 23.437428647296503 98-99 21.604982642779525 27.725464162942227 26.75506657477135 23.914486619506896 100-101 21.141078101185958 27.856078380293958 26.71706398677874 24.285779531741348 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 53.0 1 35.5 2 29.5 3 51.5 4 52.0 5 46.0 6 78.5 7 151.0 8 255.0 9 346.5 10 399.5 11 418.0 12 443.5 13 508.5 14 528.0 15 568.5 16 602.5 17 609.5 18 685.5 19 772.0 20 846.0 21 971.5 22 1138.0 23 1338.0 24 1635.0 25 2020.0 26 2601.0 27 3452.5 28 4240.5 29 5125.0 30 6212.5 31 7371.5 32 8742.0 33 9987.0 34 11227.5 35 12460.5 36 14049.0 37 15542.5 38 16549.5 39 17588.0 40 18361.5 41 19295.5 42 19794.0 43 20371.5 44 21287.5 45 21731.0 46 22389.5 47 24024.5 48 25878.0 49 25191.0 50 23317.0 51 22185.5 52 21371.5 53 20979.0 54 20784.0 55 19489.0 56 16772.0 57 14521.5 58 13228.5 59 11764.5 60 9525.0 61 7623.0 62 6126.0 63 4481.0 64 3010.5 65 2032.0 66 1522.5 67 1243.5 68 993.5 69 727.0 70 513.5 71 316.5 72 208.5 73 150.5 74 103.0 75 72.5 76 53.0 77 28.5 78 20.5 79 16.5 80 13.5 81 9.5 82 5.0 83 5.0 84 3.5 85 2.0 86 2.0 87 1.5 88 0.5 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06105677265062502 2 0.0 3 0.0 4 0.0 5 0.0013530586736980614 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005243102360579988 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.765293368490307E-4 24-25 4.2283083553064425E-4 26-27 3.3826466842451535E-4 28-29 0.0035517790184574115 30-31 3.3826466842451535E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.073970026367731E-4 40-41 8.456616710612884E-5 42-43 0.01310775590144997 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.456616710612884E-5 82-83 2.5369850131838655E-4 84-85 6.765293368490307E-4 86-87 0.0 88-89 0.0 90-91 0.010570770888266107 92-93 0.0035517790184574115 94-95 0.0 96-97 0.0 98-99 8.456616710612884E-5 100-101 0.014207116073829646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 591253.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.55614987251568 #Duplication Level Percentage of deduplicated Percentage of total 1 82.90166216565181 55.176154517777846 2 10.153765590720564 13.51591088852781 3 2.91018700746685 5.810725278780347 4 1.310404736975196 3.4886197627110254 5 0.7325321999108623 2.437726144185548 6 0.4430127978668671 1.7691135702161818 7 0.2950665398298845 1.3746944993097687 8 0.22194640522476794 1.18175185678446 9 0.1602240546169255 0.9597506573039607 >10 0.810322216190428 9.248520199096589 >50 0.0457851194359942 2.0835567631215426 >100 0.013556471251275713 1.730138635506555 >500 7.673474293154989E-4 0.3535863372962648 >1k 7.673474293154989E-4 0.8697508893820979 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1954 0.33048458105075157 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1593 0.26942780840012653 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1562 0.2641847060395465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 0.16101398217006932 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 608 0.10283245920105268 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 1.691323342122577E-4 0.0 0.0 0.0 0.0 10-11 1.691323342122577E-4 0.0 0.0 0.0 0.0 12-13 1.691323342122577E-4 0.0 0.0 0.0 0.0 14-15 1.691323342122577E-4 0.0 0.0 0.0 0.0 16-17 1.691323342122577E-4 0.0 0.0 0.0 1.691323342122577E-4 18-19 1.691323342122577E-4 0.0 0.0 0.0 1.691323342122577E-4 20-21 1.691323342122577E-4 0.0 0.0 0.0 1.691323342122577E-4 22-23 1.691323342122577E-4 0.0 0.0 1.691323342122577E-4 1.691323342122577E-4 24-25 1.691323342122577E-4 0.0 0.0 5.073970026367731E-4 1.691323342122577E-4 26-27 1.691323342122577E-4 0.0 0.0 0.0013530586736980616 1.691323342122577E-4 28-29 1.691323342122577E-4 0.0 0.0 0.0034672128513512828 1.691323342122577E-4 30-31 1.691323342122577E-4 0.0 0.0 0.008879447546143528 1.691323342122577E-4 32-33 1.691323342122577E-4 0.0 0.0 0.015813873248846096 3.382646684245154E-4 34-35 1.691323342122577E-4 0.0 0.0 0.022917431285760917 3.382646684245154E-4 36-37 1.691323342122577E-4 0.0 0.0 0.03221970966743509 3.382646684245154E-4 38-39 1.691323342122577E-4 0.0 0.0 0.04659595807547699 3.382646684245154E-4 40-41 1.691323342122577E-4 0.0 0.0 0.06832946302175211 3.382646684245154E-4 42-43 1.691323342122577E-4 0.0 0.0 0.0947141071588643 3.382646684245154E-4 44-45 1.691323342122577E-4 0.0 0.0 0.11678587677356395 3.382646684245154E-4 46-47 1.691323342122577E-4 0.0 0.0 0.16304357018061644 3.382646684245154E-4 48-49 1.691323342122577E-4 0.0 0.0 0.22088682848120855 3.382646684245154E-4 50-51 1.691323342122577E-4 0.0 0.0 0.25259914114600684 3.382646684245154E-4 52-53 1.691323342122577E-4 0.0 0.0 0.3002098932267574 3.382646684245154E-4 54-55 1.691323342122577E-4 0.0 0.0 0.38883523635398043 5.073970026367731E-4 56-57 1.691323342122577E-4 0.0 0.0 0.5382636536305101 5.073970026367731E-4 58-59 1.691323342122577E-4 0.0 0.0 0.7121316932007111 5.073970026367731E-4 60-61 1.691323342122577E-4 0.0 0.0 0.9235471109660331 5.073970026367731E-4 62-63 1.691323342122577E-4 0.0 0.0 1.1630384962105902 5.073970026367731E-4 64-65 1.691323342122577E-4 0.0 0.0 1.4252781804066956 5.073970026367731E-4 66-67 1.691323342122577E-4 0.0 0.0 1.734621219680915 5.073970026367731E-4 68-69 1.691323342122577E-4 0.0 0.0 2.088361496685852 5.073970026367731E-4 70-71 1.691323342122577E-4 0.0 0.0 2.345696343189802 6.765293368490308E-4 72-73 1.691323342122577E-4 0.0 0.0 2.6735593730602636 6.765293368490308E-4 74-75 1.691323342122577E-4 0.0 0.0 3.0544453897062676 6.765293368490308E-4 76-77 1.691323342122577E-4 0.0 0.0 3.474400975555304 6.765293368490308E-4 78-79 1.691323342122577E-4 0.0 0.0 3.9193035807006478 6.765293368490308E-4 80-81 1.691323342122577E-4 0.0 0.0 4.597524240891801 6.765293368490308E-4 82-83 1.691323342122577E-4 0.0 0.0 5.299846258708201 6.765293368490308E-4 84-85 1.691323342122577E-4 0.0 0.0 6.257896365853535 7.610955039551597E-4 86-87 1.691323342122577E-4 0.0 0.0 7.296707162585221 8.456616710612885E-4 88-89 1.691323342122577E-4 0.0 0.0 8.405285047179465 8.456616710612885E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCGTA 25 0.005017315 57.00791 7 TAAGGTG 280 0.0 32.231167 5 GTAAGGT 270 0.0 29.906504 4 GGTATCA 2740 0.0 27.921547 1 ACGACCC 90 8.9351356E-4 26.392555 8 GTGTAGG 235 1.7462298E-10 24.264845 1 GGTAAGG 345 0.0 23.40509 3 GGACCAT 285 3.6379788E-12 23.332626 6 AACGACC 105 0.002193198 22.622189 7 GAGTACT 2040 0.0 22.588923 12-13 GTACTTT 2020 0.0 22.573574 14-15 GTATCAA 3600 0.0 22.439365 1 AGGTAAG 340 0.0 22.352264 2 GGTGTGC 505 0.0 21.636667 8 TCAACGC 4040 0.0 19.751877 4 ATCAACG 4050 0.0 19.703106 3 GTACATG 1885 0.0 19.662891 1 GTCCTAT 145 6.124977E-4 19.662891 1 TAAGGGT 170 7.9473706E-5 19.558231 4 TATCAAC 4145 0.0 19.480713 2 >>END_MODULE