FastQCFastQC Report
Thu 26 May 2016
SRR937287_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937287_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577638
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33360.577524331847974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25670.44439597117918145No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16620.28772345309692227No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9000.1558069240597052No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8840.15303702318753268No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6820.11806702467635441No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6550.11339281695456323No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43400.030.0007551
GGACCGT804.4892574E-429.6893426
GGTATCA30150.029.3154771
CGGTATA350.00839081527.14336634-35
ATAGCAC1257.3789743E-626.6016523
GTACATG35100.025.7227751
TACATGG35350.025.128862
ATCAACG53150.024.3994483
TCAACGC53650.024.26274
TATCAAC53600.024.1059762
GAGTACT26000.023.93210212-13
CAACGCA54450.023.8189755
GTCTAGG1601.850276E-623.7597221
GTATAGG1601.850276E-623.7597221
AACGCAG55300.023.3649236
ACATGGG35800.023.2207163
CATGGGA23950.022.017894
GTACTTT28150.021.59804714-15
AGTACTT27250.021.35268612-13
ACGCAGA60100.021.1826827
Produced by FastQC (version 0.11.5)