##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937287_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 577638 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86691665022038 33.0 31.0 34.0 30.0 34.0 2 31.993160076033778 33.0 31.0 34.0 30.0 34.0 3 32.019654177876106 34.0 31.0 34.0 30.0 34.0 4 35.63434884824059 37.0 35.0 37.0 35.0 37.0 5 35.64411621119109 37.0 35.0 37.0 35.0 37.0 6 35.69982930485875 37.0 35.0 37.0 35.0 37.0 7 35.6588693957115 37.0 35.0 37.0 33.0 37.0 8 35.64838878328642 37.0 35.0 37.0 33.0 37.0 9 37.26390923034842 39.0 37.0 39.0 34.0 39.0 10-11 37.158086033121094 39.0 37.0 39.0 34.0 39.0 12-13 37.03015383336969 39.0 37.0 39.0 33.0 39.0 14-15 38.13930177723765 40.0 38.0 41.0 33.0 41.0 16-17 38.06727569862093 40.0 38.0 41.0 33.0 41.0 18-19 38.01341584175556 40.0 38.0 41.0 33.0 41.0 20-21 37.90790772075245 40.0 38.0 41.0 33.0 41.0 22-23 37.9817048047393 40.0 38.0 41.0 33.0 41.0 24-25 37.959600130185336 40.0 38.0 41.0 33.0 41.0 26-27 37.867975271709966 40.0 38.0 41.0 33.0 41.0 28-29 37.733046475474254 40.0 37.5 41.0 33.0 41.0 30-31 37.477124081171944 40.0 37.0 41.0 32.5 41.0 32-33 37.20746297854366 39.0 36.5 41.0 32.0 41.0 34-35 37.183096506808766 39.5 36.0 41.0 32.0 41.0 36-37 37.03812768550546 39.5 36.0 41.0 31.0 41.0 38-39 36.89298747658569 39.0 36.0 41.0 31.0 41.0 40-41 36.65862876057323 39.0 36.0 41.0 30.5 41.0 42-43 36.36663186978696 39.0 35.0 41.0 30.0 41.0 44-45 36.07509374383264 39.0 35.0 40.0 29.5 41.0 46-47 35.61067052375363 39.0 35.0 40.0 27.5 41.0 48-49 35.212563058524545 39.0 35.0 40.0 26.0 41.0 50-51 34.276827355541016 37.5 34.0 39.5 24.5 40.5 52-53 34.39005138166118 38.0 34.0 39.5 24.0 40.5 54-55 34.68327395358338 38.0 34.0 40.0 24.0 41.0 56-57 34.40235320390971 38.0 34.0 40.0 23.0 41.0 58-59 34.13331706016571 38.0 34.0 40.0 22.5 41.0 60-61 33.79712120739979 38.0 33.5 40.0 21.5 41.0 62-63 33.402753973942154 37.5 33.0 40.0 19.5 41.0 64-65 32.97644805224033 37.0 32.0 39.5 18.0 41.0 66-67 32.50233017910872 36.0 31.5 39.0 14.0 40.5 68-69 31.986091635245604 36.0 31.0 39.0 9.0 40.0 70-71 31.496928872407977 35.0 31.0 38.5 7.5 40.0 72-73 30.978472676659084 35.0 30.0 38.0 4.0 40.0 74-75 30.401158684158588 35.0 29.5 37.0 2.0 39.0 76-77 29.84078869464959 34.0 29.0 36.5 2.0 39.0 78-79 29.2472136528414 34.0 29.0 36.0 2.0 38.5 80-81 28.610084516600363 34.0 27.5 35.5 2.0 37.5 82-83 28.01487523327759 33.5 26.5 35.0 2.0 37.0 84-85 27.366491643555307 33.0 26.0 35.0 2.0 36.5 86-87 26.726467960902852 32.5 25.0 35.0 2.0 36.0 88-89 26.092386581215223 32.0 23.5 35.0 2.0 35.5 90-91 25.45939238761993 32.0 20.5 34.5 2.0 35.0 92-93 24.73541300953192 31.0 18.0 34.0 2.0 35.0 94-95 23.895044993577294 31.0 4.5 34.0 2.0 35.0 96-97 22.98141915871186 31.0 2.0 34.0 2.0 35.0 98-99 21.72788753509984 30.0 2.0 34.0 2.0 35.0 100-101 18.643575214926997 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09756723879077 1101 2 0.07175786775066584 1101 3 -0.03657325536894618 1101 4 -0.13376006342728175 1101 5 -0.03878398898380908 1101 6 2.292585376721945E-4 1101 7 0.05538016242577726 1101 8 -0.05192382974219356 1101 9 -0.012450543124508329 1101 10-11 -0.009250411954710103 1101 12-13 0.12213802539506702 1101 14-15 0.30875381768684207 1101 16-17 0.35916913910474335 1101 18-19 0.5342748111786761 1101 20-21 0.42251377089959163 1101 22-23 0.4727081252001355 1101 24-25 0.47361489612006835 1101 26-27 0.19080984791760613 1101 28-29 0.3933947198738039 1101 30-31 0.31241186839097423 1101 32-33 0.12041680806332522 1101 34-35 0.3770175285728712 1101 36-37 0.34153127137921757 1101 38-39 0.4473008035150343 1101 40-41 0.3988789918479725 1101 42-43 0.1481893885579595 1101 44-45 0.167255312442542 1101 46-47 0.36109445515401717 1101 48-49 0.43283684198421213 1101 50-51 0.614517749102319 1101 52-53 0.6046828494227796 1101 54-55 0.6715526865218919 1101 56-57 0.703383005790279 1101 58-59 0.9005731706253499 1101 60-61 0.6910475228531681 1101 62-63 0.6414317709291311 1101 64-65 0.6815286327260992 1101 66-67 0.9735169916765045 1101 68-69 0.9950157181940682 1101 70-71 0.7839494571930281 1101 72-73 0.8524836872510448 1101 74-75 1.0986876349362653 1101 76-77 0.8393258342280632 1101 78-79 1.1330957004910758 1101 80-81 1.4506067213517575 1101 82-83 1.501589947092942 1101 84-85 1.7994116354230911 1101 86-87 2.0736136118705026 1101 88-89 2.107735383962961 1101 90-91 1.870218326878998 1101 92-93 2.1651697238799805 1101 94-95 2.496102194187518 1101 96-97 2.3054876157652657 1101 98-99 2.744756659780858 1101 100-101 2.6482527845797357 1102 1 0.30718103277181186 1102 2 0.32310990694389474 1102 3 0.18366597603653645 1102 4 0.21076338252296267 1102 5 0.20655545238540896 1102 6 0.22166941887361702 1102 7 0.03732646261028805 1102 8 0.20631565616478298 1102 9 0.3585649178413206 1102 10-11 0.39782472961174875 1102 12-13 0.35990207069633584 1102 14-15 0.4107055260281314 1102 16-17 0.46850536739258075 1102 18-19 0.5266715790096868 1102 20-21 0.503091330230248 1102 22-23 0.556848705463544 1102 24-25 0.5652672470776352 1102 26-27 0.6056274021582411 1102 28-29 0.5095666992591816 1102 30-31 0.6803534284851409 1102 32-33 0.5374612503869258 1102 34-35 0.6803316152306351 1102 36-37 0.9090576759093452 1102 38-39 0.6621955035214029 1102 40-41 0.7890496669131863 1102 42-43 0.7104150041334591 1102 44-45 0.7583748008945363 1102 46-47 0.686065028418632 1102 48-49 0.8384446233674296 1102 50-51 0.6256999085475101 1102 52-53 0.5675733501544684 1102 54-55 0.6093628602191643 1102 56-57 0.786485697143597 1102 58-59 0.8299370759507951 1102 60-61 1.0234264755795124 1102 62-63 1.0155251093524953 1102 64-65 0.6999163657540208 1102 66-67 0.8378875776661943 1102 68-69 0.9648891037002016 1102 70-71 1.0357468740028501 1102 72-73 1.004309735479083 1102 74-75 1.0161217826743858 1102 76-77 1.271783523354486 1102 78-79 1.5735184910999713 1102 80-81 1.637223919290296 1102 82-83 1.226680613250604 1102 84-85 1.5872448733183653 1102 86-87 1.7735444192764938 1102 88-89 2.027738406168215 1102 90-91 1.9109823085548925 1102 92-93 2.2351576350589575 1102 94-95 2.29813184361171 1102 96-97 2.344617220651692 1102 98-99 2.311583185199197 1102 100-101 1.8160726611465101 1103 1 0.2587160786323075 1103 2 0.20772278003136435 1103 3 0.2695953920560683 1103 4 0.2036055279705664 1103 5 0.2206953545362822 1103 6 0.22073010743410748 1103 7 0.29250523316714805 1103 8 0.2705153326799916 1103 9 0.33856813259705376 1103 10-11 0.4343687165116208 1103 12-13 0.12790344701362955 1103 14-15 0.28583361395169504 1103 16-17 0.3091903615156042 1103 18-19 0.41380225883006005 1103 20-21 0.28587681560813394 1103 22-23 0.21156635949554214 1103 24-25 0.3083630568444633 1103 26-27 0.2973109797795246 1103 28-29 0.1705594057821216 1103 30-31 0.5318869674288962 1103 32-33 0.445155064995987 1103 34-35 0.5549674436832319 1103 36-37 0.6006922787363251 1103 38-39 0.6477818448279962 1103 40-41 0.9576296992616662 1103 42-43 0.965252206894128 1103 44-45 1.0694199983508597 1103 46-47 1.0875729497776732 1103 48-49 1.2161206563588607 1103 50-51 1.1163570247106875 1103 52-53 1.235766607204333 1103 54-55 1.3539593079140815 1103 56-57 1.1755816475559868 1103 58-59 1.194821897280704 1103 60-61 0.9658070021966836 1103 62-63 1.0593010408781964 1103 64-65 1.116092865889648 1103 66-67 1.4744720001654699 1103 68-69 1.5973784799371344 1103 70-71 1.548875886168691 1103 72-73 1.7649058717445385 1103 74-75 1.7713319473301574 1103 76-77 1.5428841251165757 1103 78-79 1.7317799110173908 1103 80-81 1.490186517616614 1103 82-83 1.6794337501489665 1103 84-85 1.5238740010144873 1103 86-87 1.9192205728212635 1103 88-89 1.957516085717348 1103 90-91 1.8805111961489445 1103 92-93 2.4538652530254232 1103 94-95 2.292508532614228 1103 96-97 2.0909290419112736 1103 98-99 1.8376754486886142 1103 100-101 1.8296336561134083 1104 1 0.10372935624321045 1104 2 0.13011187933048163 1104 3 0.03185795499774713 1104 4 0.12706844498187309 1104 5 0.08117741162275394 1104 6 0.08314213419248517 1104 7 0.19968675189365825 1104 8 0.08685477516544182 1104 9 0.12365367523777593 1104 10-11 0.21434346728433695 1104 12-13 0.20659832316182047 1104 14-15 0.2542459599531739 1104 16-17 0.27299254687976315 1104 18-19 0.23342700307279785 1104 20-21 0.27413012049981234 1104 22-23 0.22374548389878157 1104 24-25 0.08334745746552841 1104 26-27 0.13920728381504688 1104 28-29 0.09870213647153747 1104 30-31 0.05563284991841044 1104 32-33 0.16898656268146794 1104 34-35 0.416771457439701 1104 36-37 0.54370593363452 1104 38-39 0.7268569104904969 1104 40-41 0.7158248148421862 1104 42-43 0.6660691790184003 1104 44-45 0.774844228902424 1104 46-47 0.9463543117848445 1104 48-49 0.9404498830649928 1104 50-51 0.8431226567394816 1104 52-53 0.8410283139940518 1104 54-55 1.2245338003900983 1104 56-57 0.9188985834026298 1104 58-59 1.0870047945569752 1104 60-61 1.0490017550009512 1104 62-63 0.7694370094182403 1104 64-65 0.861339771408197 1104 66-67 0.8752643035044585 1104 68-69 0.635648472536495 1104 70-71 0.6137054538845099 1104 72-73 0.9172617407387804 1104 74-75 0.7435215196895051 1104 76-77 1.0207808935057052 1104 78-79 0.9973843688120034 1104 80-81 0.8138511382252069 1104 82-83 0.7973577993124046 1104 84-85 0.6165676872565626 1104 86-87 0.7364306783166015 1104 88-89 0.6695530018289695 1104 90-91 0.709848186266921 1104 92-93 0.9181129276296325 1104 94-95 1.0866591282928404 1104 96-97 1.3492358925780579 1104 98-99 1.0749296677764448 1104 100-101 0.705076605919686 1105 1 0.19039411651391802 1105 2 0.08805678448728216 1105 3 0.20226433506955743 1105 4 0.16481190763239795 1105 5 0.2134844684651256 1105 6 0.021409855731583605 1105 7 0.0344943647681859 1105 8 0.04072317029777395 1105 9 0.32185213105704946 1105 10-11 0.37607256841863546 1105 12-13 0.43336888840821786 1105 14-15 0.5374982714582686 1105 16-17 0.4790501200377122 1105 18-19 0.439594193998353 1105 20-21 0.32771573356497896 1105 22-23 0.38157759396137436 1105 24-25 0.4431955812541801 1105 26-27 0.2610545814987759 1105 28-29 0.31211998669235896 1105 30-31 0.2737356682071024 1105 32-33 0.3766200898317251 1105 34-35 0.11789623105518388 1105 36-37 0.42983372571027445 1105 38-39 0.3474586961480526 1105 40-41 0.19194720546271782 1105 42-43 0.0692830448716748 1105 44-45 0.27055007200603853 1105 46-47 0.4926574922887639 1105 48-49 0.42191177103246247 1105 50-51 0.0350617791618788 1105 52-53 0.025721543111068 1105 54-55 0.37491190225519233 1105 56-57 0.535182382214316 1105 58-59 0.27812893270713346 1105 60-61 0.37292384695278 1105 62-63 0.49466657126490077 1105 64-65 0.6014281010876061 1105 66-67 0.25822588962589776 1105 68-69 0.3465816533581574 1105 70-71 0.5155069980652307 1105 72-73 0.5437336372850226 1105 74-75 0.42085606040694756 1105 76-77 0.36748918315079493 1105 78-79 0.8490238693362606 1105 80-81 0.878678132791979 1105 82-83 1.1723538276541596 1105 84-85 1.2062699717543843 1105 86-87 1.1894173653181568 1105 88-89 1.4262063237239246 1105 90-91 1.5579131943691031 1105 92-93 1.7337580837952373 1105 94-95 1.8752143978890032 1105 96-97 2.055652503641511 1105 98-99 1.733576762111273 1105 100-101 1.6316041056813866 1106 1 0.2601924202577628 1106 2 -0.2826440908625578 1106 3 0.20345528723581197 1106 4 0.3147740523671132 1106 5 0.28366132486181783 1106 6 0.2766096379574705 1106 7 0.369496448934477 1106 8 0.4014668348575938 1106 9 0.5455512284854436 1106 10-11 -0.22961592953547694 1106 12-13 0.10361762727495005 1106 14-15 0.2064433430430057 1106 16-17 0.21267452775167328 1106 18-19 0.46931270394706104 1106 20-21 0.40915134133339137 1106 22-23 0.35971724160966545 1106 24-25 0.07473199461666269 1106 26-27 0.3576195351792393 1106 28-29 0.5541129600745833 1106 30-31 0.6482965045957982 1106 32-33 0.6597413202554492 1106 34-35 0.7835789443844945 1106 36-37 0.6457576068532447 1106 38-39 0.8327674504971441 1106 40-41 0.7497977069386437 1106 42-43 0.47935126941719375 1106 44-45 0.5215496211455743 1106 46-47 0.8695172916983935 1106 48-49 0.9525936762156562 1106 50-51 0.6724210979372387 1106 52-53 0.5995371837157322 1106 54-55 0.8605290281473401 1106 56-57 0.3790953406245663 1106 58-59 0.5694804932798121 1106 60-61 0.47248744805185794 1106 62-63 0.5943641544826903 1106 64-65 0.436089913206402 1106 66-67 0.6600850510254404 1106 68-69 0.8221884009528537 1106 70-71 0.8352861615063993 1106 72-73 1.0495076285910727 1106 74-75 0.8543392552212019 1106 76-77 0.8201041738358512 1106 78-79 1.0942217800658902 1106 80-81 1.3942243119622226 1106 82-83 1.4367600954652744 1106 84-85 0.9416786810174429 1106 86-87 0.8633410422979004 1106 88-89 1.3329866696104595 1106 90-91 1.243580510388263 1106 92-93 1.8525513089787715 1106 94-95 1.7661189260592707 1106 96-97 1.9494216971116884 1106 98-99 2.1126440764290706 1106 100-101 1.5804271897370903 1107 1 0.16426296677682117 1107 2 0.18219088140948614 1107 3 0.15806493120471998 1107 4 0.11562238353580767 1107 5 0.048617929063269116 1107 6 0.1154937165440657 1107 7 0.2208117230186275 1107 8 -0.08984885153818567 1107 9 -0.009471757887659749 1107 10-11 0.18625961420048043 1107 12-13 0.2741832157467101 1107 14-15 0.3413388220460334 1107 16-17 0.4071748060620237 1107 18-19 0.15967440432020652 1107 20-21 0.23919133556102423 1107 22-23 0.46865770381099736 1107 24-25 0.29121879033686326 1107 26-27 0.23529182989959452 1107 28-29 0.33621657398597904 1107 30-31 0.39013273441829455 1107 32-33 0.17939312308803324 1107 34-35 0.41815745882571065 1107 36-37 0.48787317780176664 1107 38-39 0.6832787169123051 1107 40-41 0.7529003519177166 1107 42-43 0.8153646283138514 1107 44-45 0.7840091630673598 1107 46-47 0.8275104679410035 1107 48-49 0.48454782716293465 1107 50-51 0.591522980139807 1107 52-53 0.579980727946463 1107 54-55 0.8590163598726548 1107 56-57 0.9846239241279804 1107 58-59 1.1286137111658903 1107 60-61 1.0575834135826057 1107 62-63 0.7503390653203041 1107 64-65 0.7363455464139719 1107 66-67 1.15927331251347 1107 68-69 1.3303470172350416 1107 70-71 1.0675516077306604 1107 72-73 1.1878149862920253 1107 74-75 1.341985368153356 1107 76-77 1.4736799464047579 1107 78-79 1.43442525585289 1107 80-81 1.4323889067629771 1107 82-83 1.381239758194365 1107 84-85 1.2590890547779274 1107 86-87 1.577918319804244 1107 88-89 1.629549536825504 1107 90-91 1.4920034184221507 1107 92-93 1.4630482475649522 1107 94-95 1.5353713020050144 1107 96-97 1.3968854652276335 1107 98-99 1.9860927539395306 1107 100-101 2.22515630099938 1108 1 0.5127000205452852 1108 2 0.43295677698806045 1108 3 0.4162171756682085 1108 4 0.40579944820855474 1108 5 0.4789409033343688 1108 6 0.5130000124653478 1108 7 0.50553664967439 1108 8 0.41833584612778196 1108 9 0.5421418153397468 1108 10-11 0.5330902344460853 1108 12-13 0.6364291071712742 1108 14-15 0.793136080399492 1108 16-17 0.6092446713163255 1108 18-19 0.703625776441605 1108 20-21 0.8507329924917286 1108 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0.3977029448786098 2316 62-63 0.21607686194045073 2316 64-65 0.1280902322763069 2316 66-67 0.24040591570489056 2316 68-69 0.5513179882060086 2316 70-71 0.6786627188417746 2316 72-73 0.795223865759727 2316 74-75 0.7560906499056976 2316 76-77 0.7340874126945778 2316 78-79 0.3958425555054852 2316 80-81 0.7884950128690811 2316 82-83 1.2356014728501954 2316 84-85 1.287139163710389 2316 86-87 1.7333973282244273 2316 88-89 1.7082826817351169 2316 90-91 1.6910112497829246 2316 92-93 2.037218005558241 2316 94-95 2.5071887076459483 2316 96-97 2.2277678181688074 2316 98-99 2.4979060378116387 2316 100-101 3.334773270910471 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2323.0 3 375.0 4 369.0 5 418.0 6 491.0 7 577.0 8 711.0 9 864.0 10 1081.0 11 1494.0 12 2074.0 13 2697.0 14 3081.0 15 4036.0 16 5470.0 17 7550.0 18 6398.0 19 4437.0 20 4340.0 21 4514.0 22 4915.0 23 5725.0 24 6452.0 25 7245.0 26 8427.0 27 9878.0 28 11713.0 29 13916.0 30 16680.0 31 20664.0 32 25865.0 33 33461.0 34 44384.0 35 60047.0 36 81234.0 37 100336.0 38 67158.0 39 6238.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.207951734016234 19.909371066452536 15.20256955597031 21.68010764356092 2 14.673657428378537 20.774086280716915 41.616726456076044 22.935529834828504 3 18.71460628916846 26.410064613318013 30.555695361135164 24.319633736378357 4 11.774149041223318 17.183527333248872 39.09837331634858 31.943950309179233 5 12.41120658385934 38.518596938199956 34.866200275253085 14.203996202687621 6 29.28793633223276 37.441074870698706 18.633440151847694 14.637548645220841 7 26.806518717978523 33.99667922223706 21.69036957098813 17.50643248879628 8 25.671410799828582 34.73656267512896 20.530535549768285 19.06149097527418 9 26.273368661787316 16.94672621836316 21.310206730452226 35.469698389397294 10-11 23.85873215020121 27.614456493257467 27.96160460342168 20.565206753119647 12-13 25.081249815637097 25.363650402822095 28.67871228802604 20.876387493514763 14-15 22.115344576306477 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31.32397825764534 20.81291843494597 40-41 20.509655898571868 27.467661890033945 31.34502599364773 20.677656217746453 42-43 20.4563217204316 27.464832104036425 30.879307996776618 21.199538178755358 44-45 20.500888216558298 27.598467132517747 30.64086906438876 21.25977558653519 46-47 20.697522307215863 27.8607280228598 30.31430489795848 21.127444771965862 48-49 20.702745797501013 28.050580069349802 29.984289155873956 21.262384977275232 50-51 20.602099487491834 28.206950225019455 30.02220261381768 21.16874767367103 52-53 20.857050632384176 28.422059170611835 29.46547571117189 21.255414485832095 54-55 20.574561574730744 28.228037529116296 29.68166183021675 21.51573906593621 56-57 20.76042006369648 28.434006841473487 29.45875340859417 21.34681968623587 58-59 20.94082508219341 28.129355209947676 29.624490383522982 21.305329324335926 60-61 20.71121433564432 28.14372763524659 30.108009942395853 21.037048086713234 62-63 20.65835647662282 28.17612375776478 29.98832572105585 21.17719404455654 64-65 20.494679421023875 28.179642172634793 29.881707094972555 21.44397131136878 66-67 20.445090089113418 28.280826521541165 29.572992779861668 21.70109060948375 68-69 20.5103920568675 28.210852422731737 29.77642593729727 21.502329583103496 70-71 20.58628639029442 28.281609663950817 29.6213483945165 21.510755551238265 72-73 20.639655149982868 28.222972943671316 29.462819773366927 21.674552132978885 74-75 20.342914040775685 28.515484733837436 29.41683102879416 21.724770196592715 76-77 20.40299579464546 28.179605418384202 29.590932117527863 21.82646666944248 78-79 20.623047717027074 27.983539451621546 29.380031696270958 22.013381135080415 80-81 20.506264116379686 28.18357972306742 29.301007185258943 22.00914897529395 82-83 20.636733745136507 28.21614983113294 29.468732274163077 21.678384149567467 84-85 20.476442378569633 28.063618152333213 29.473519898016704 21.98641957108045 86-87 20.36228408782445 28.047747193802998 29.389986583676837 22.199982134695723 88-89 20.550567411072063 27.828468736014212 29.282990851342117 22.337973001571612 90-91 20.22284610574392 27.628701995588873 29.66071780140903 22.48773409725818 92-93 20.39320926028885 27.562118198700176 29.75957631168777 22.285096229323205 94-95 20.131328226028174 27.370165717105717 29.909218561495383 22.589287495370726 96-97 20.078047951176227 27.5138544220956 29.913321496320954 22.494776130407214 98-99 19.993234217511233 27.77518519161043 29.42620960047187 22.805370990406466 100-101 20.394573658201523 27.0656229348107 29.93707080921597 22.60273259777181 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 839.0 1 478.0 2 135.0 3 161.0 4 143.5 5 139.0 6 221.5 7 438.0 8 765.0 9 987.0 10 949.5 11 890.0 12 948.0 13 1008.0 14 1066.5 15 1132.0 16 1232.5 17 1319.5 18 1362.0 19 1437.5 20 1536.5 21 1753.0 22 2062.0 23 2388.0 24 2807.5 25 3362.5 26 4183.5 27 5225.5 28 6185.5 29 7251.0 30 8626.0 31 9941.0 32 11353.0 33 13015.5 34 14883.5 35 16449.5 36 18241.5 37 19982.5 38 20530.0 39 20592.5 40 20407.5 41 20522.5 42 20865.5 43 21180.5 44 21785.0 45 22136.5 46 21992.5 47 21956.0 48 22942.5 49 22470.0 50 19827.0 51 17620.5 52 16172.0 53 15208.5 54 14096.0 55 12677.5 56 11070.5 57 9488.5 58 8178.5 59 7021.5 60 5919.5 61 4719.5 62 3602.5 63 2691.5 64 1997.0 65 1507.5 66 1073.0 67 755.0 68 569.0 69 422.5 70 314.0 71 231.5 72 171.0 73 124.0 74 99.5 75 77.5 76 46.0 77 27.5 78 18.5 79 13.5 80 8.5 81 5.5 82 3.0 83 3.5 84 3.0 85 3.0 86 2.0 87 1.0 88 1.5 89 2.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2878965719014331 2 0.25084914773612543 3 0.24946419730003913 4 0.2484254844729744 5 0.24946419730003913 6 0.22003400053320593 7 0.2193415253151628 8 0.22107271336027062 9 0.2305942476083637 10-11 0.23734588098428427 12-13 0.23007489119483138 14-15 0.2212458321647814 16-17 0.21726409966103338 18-19 0.20497266454076774 20-21 0.2128495701460084 22-23 0.20705009019489715 24-25 0.21111838210090056 26-27 0.20618449617234325 28-29 0.221159272762526 30-31 0.2318926386421946 32-33 0.2245350894504863 34-35 0.22289046080763383 36-37 0.21233021373247607 38-39 0.20073125383025356 40-41 0.1992597439919119 42-43 0.21198397612345446 44-45 0.20808880302196184 46-47 0.21726409966103338 48-49 0.22237110439410152 50-51 0.23448942070985632 52-53 0.2434050391421617 54-55 0.22254422319861228 56-57 0.19977910040544425 58-59 0.1906903631686281 60-61 0.19337370463854525 62-63 0.2002984568189766 64-65 0.19095004137539429 66-67 0.1948452144768869 68-69 0.19735543714229326 70-71 0.1982210311648472 72-73 0.20003877861221042 74-75 0.2020296448640844 76-77 0.2129361295482638 78-79 0.2128495701460084 80-81 0.2042801893227246 82-83 0.1989135063828903 84-85 0.18575647724007077 86-87 0.1906903631686281 88-89 0.20081781323250894 90-91 0.19761511534905946 92-93 0.2036742735069369 94-95 0.197961352958081 96-97 0.20730976840166332 98-99 0.20912751584902656 100-101 0.19337370463854525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 577638.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.3099097371455 #Duplication Level Percentage of deduplicated Percentage of total 1 86.02445469538542 59.62347190124326 2 8.57895943446027 11.892138080821484 3 2.227957376928618 4.6325857407939 4 0.9752990462526508 2.703915554499813 5 0.515756763650588 1.787352736747227 6 0.3290083062066097 1.3682121603570747 7 0.22158684087366431 1.075071475792503 8 0.1734786147769901 0.9619029705214576 9 0.14286137828994327 0.8911538310780152 >10 0.754718201405569 9.582337035639783 >50 0.03861695470472513 1.8273449290661505 >100 0.015547072435153545 1.7972761195782498 >500 0.001003036931297992 0.5424329145339688 >1k 7.522776984734941E-4 1.3148045493269702 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3336 0.577524331847974 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2567 0.44439597117918145 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1662 0.28772345309692227 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 900 0.1558069240597052 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 884 0.15303702318753268 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 682 0.11806702467635441 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 655 0.11339281695456323 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.7311880451078356E-4 0.0 22-23 0.0 0.0 0.0 6.059158157877425E-4 0.0 24-25 0.0 0.0 0.0 9.521534248093097E-4 0.0 26-27 0.0 0.0 0.0 0.001644628642852444 0.0 28-29 8.655940225539178E-5 0.0 0.0 0.0032026978834494963 0.0 30-31 1.7311880451078356E-4 0.0 0.0 0.009521534248093096 0.0 32-33 1.7311880451078356E-4 0.0 0.0 0.018177474473632275 1.7311880451078356E-4 34-35 1.7311880451078356E-4 0.0 0.0 0.025448464263085183 1.7311880451078356E-4 36-37 1.7311880451078356E-4 0.0 0.0 0.031680741225473395 1.7311880451078356E-4 38-39 1.7311880451078356E-4 0.0 0.0 0.03817269639462778 1.7311880451078356E-4 40-41 1.7311880451078356E-4 0.0 0.0 0.0484732652630194 1.7311880451078356E-4 42-43 1.7311880451078356E-4 0.0 0.0 0.062322769623882085 1.7311880451078356E-4 44-45 1.7311880451078356E-4 0.0 0.0 0.07496044235316929 1.7311880451078356E-4 46-47 1.7311880451078356E-4 0.0 0.0 0.09045457535688442 1.7311880451078356E-4 48-49 1.7311880451078356E-4 0.0 0.0 0.10603526776285493 1.7311880451078356E-4 50-51 1.7311880451078356E-4 0.0 0.0 0.1254245738680627 1.7311880451078356E-4 52-53 1.7311880451078356E-4 0.0 0.0 0.18264033875887667 1.7311880451078356E-4 54-55 1.7311880451078356E-4 0.0 0.0 0.26963253802554543 1.7311880451078356E-4 56-57 1.7311880451078356E-4 0.0 0.0 0.3443333021719485 1.7311880451078356E-4 58-59 1.7311880451078356E-4 0.0 0.0 0.46326592087085683 1.7311880451078356E-4 60-61 1.7311880451078356E-4 0.0 0.0 0.6208905923779253 1.7311880451078356E-4 62-63 1.7311880451078356E-4 0.0 0.0 0.728397369979122 1.7311880451078356E-4 64-65 1.7311880451078356E-4 0.0 0.0 0.8027518965165035 1.7311880451078356E-4 66-67 1.7311880451078356E-4 0.0 0.0 1.0081573580685481 1.7311880451078356E-4 68-69 1.7311880451078356E-4 0.0 0.0 1.2218725222371105 1.7311880451078356E-4 70-71 1.7311880451078356E-4 0.0 0.0 1.3365637302255045 1.7311880451078356E-4 72-73 3.462376090215671E-4 0.0 0.0 1.5791031753451124 1.7311880451078356E-4 74-75 3.462376090215671E-4 0.0 0.0 1.9204068984381222 1.7311880451078356E-4 76-77 3.462376090215671E-4 0.0 0.0 2.267683220286754 1.7311880451078356E-4 78-79 3.462376090215671E-4 0.0 0.0 2.591328825319664 1.7311880451078356E-4 80-81 3.462376090215671E-4 0.0 0.0 2.9433659142923423 1.7311880451078356E-4 82-83 3.462376090215671E-4 0.0 0.0 3.282851889937989 1.7311880451078356E-4 84-85 4.327970112769589E-4 0.0 0.0 3.6683874675835035 1.7311880451078356E-4 86-87 5.193564135323507E-4 0.0 0.0 4.348311572299606 1.7311880451078356E-4 88-89 5.193564135323507E-4 0.0 0.0 5.111073024974119 1.7311880451078356E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4340 0.0 30.000755 1 GGACCGT 80 4.4892574E-4 29.689342 6 GGTATCA 3015 0.0 29.315477 1 CGGTATA 35 0.008390815 27.143366 34-35 ATAGCAC 125 7.3789743E-6 26.601652 3 GTACATG 3510 0.0 25.722775 1 TACATGG 3535 0.0 25.12886 2 ATCAACG 5315 0.0 24.399448 3 TCAACGC 5365 0.0 24.2627 4 TATCAAC 5360 0.0 24.105976 2 GAGTACT 2600 0.0 23.932102 12-13 CAACGCA 5445 0.0 23.818975 5 GTCTAGG 160 1.850276E-6 23.759722 1 GTATAGG 160 1.850276E-6 23.759722 1 AACGCAG 5530 0.0 23.364923 6 ACATGGG 3580 0.0 23.220716 3 CATGGGA 2395 0.0 22.01789 4 GTACTTT 2815 0.0 21.598047 14-15 AGTACTT 2725 0.0 21.352686 12-13 ACGCAGA 6010 0.0 21.182682 7 >>END_MODULE