FastQCFastQC Report
Thu 26 May 2016
SRR937287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937287_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577638
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45520.7880367981330868No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39330.6808762581409118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.42881527877321085No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13790.23873083142037055No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10040.1738112797288267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.11044979727787992No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT600.00394955131.6663196
CGTCGAT300.003951172331.66631944-45
GGTATCA38950.030.7446771
GTATTAC1102.7213773E-630.2400321
GTATCAA55450.029.1376321
ACGATGC700.00838508127.144918
CGTCTCA908.93819E-426.3908848
GTACATG36250.025.6935981
GAGTACT38900.024.54555912-13
TACATGG37700.024.3167112
GTACTTT39700.023.9890314-15
ATCAACG67800.023.8898563
CTGGTCG1000.001651925323.7517979
ATTCGAT601.7109506E-423.7497484-85
TCAACGC68600.023.611264
TATCAAC68750.023.5597422
CAACGCA69050.023.3885945
ACATGGG37500.023.306413
TACCTGG3900.023.1407722
AACGCAG70250.023.0566866
Produced by FastQC (version 0.11.5)