##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937287_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 577638 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41799015992715 33.0 31.0 34.0 31.0 34.0 2 32.60708609890624 34.0 31.0 34.0 31.0 34.0 3 32.662510084170364 34.0 31.0 34.0 31.0 34.0 4 36.252663432807395 37.0 35.0 37.0 35.0 37.0 5 36.212719731042625 37.0 35.0 37.0 35.0 37.0 6 36.27652959119725 37.0 37.0 37.0 35.0 37.0 7 36.225061370616196 37.0 37.0 37.0 35.0 37.0 8 36.2164262046472 37.0 36.0 37.0 35.0 37.0 9 37.940895508951975 39.0 38.0 39.0 35.0 39.0 10-11 37.86942946966785 39.0 38.0 39.0 35.0 39.0 12-13 37.77470578459173 39.0 37.0 39.0 35.0 39.0 14-15 39.01333793829353 40.0 38.0 41.0 36.0 41.0 16-17 38.97974509987223 40.0 38.0 41.0 36.0 41.0 18-19 39.00837721895027 40.0 38.0 41.0 36.0 41.0 20-21 38.93439836021868 40.0 38.0 41.0 35.5 41.0 22-23 38.88602723505032 40.0 38.0 41.0 35.0 41.0 24-25 38.91365786184427 40.0 38.0 41.0 35.0 41.0 26-27 38.771568871854 40.0 38.0 41.0 35.0 41.0 28-29 38.62119960944398 40.0 38.0 41.0 35.0 41.0 30-31 38.61915074839259 40.0 38.0 41.0 35.0 41.0 32-33 38.467786918450656 40.0 38.0 41.0 35.0 41.0 34-35 38.32944335379597 40.0 38.0 41.0 34.0 41.0 36-37 38.167546110193584 40.0 38.0 41.0 34.0 41.0 38-39 38.04483950155634 40.0 37.5 41.0 34.0 41.0 40-41 37.83952579297068 40.0 37.0 41.0 33.0 41.0 42-43 37.60511341012884 40.0 37.0 41.0 33.0 41.0 44-45 37.22155831160693 40.0 37.0 41.0 33.0 41.0 46-47 36.74721451843542 40.0 37.0 41.0 31.5 41.0 48-49 36.31120528774076 40.0 36.0 41.0 30.5 41.0 50-51 36.021572334230086 39.0 36.0 41.0 29.5 41.0 52-53 35.780455925683555 39.0 35.5 41.0 29.0 41.0 54-55 35.570269788344945 39.0 35.0 40.0 28.0 41.0 56-57 35.282419092926716 39.0 35.0 40.0 27.5 41.0 58-59 35.01923436477517 38.0 35.0 40.0 27.0 41.0 60-61 34.67651712664333 38.0 34.0 40.0 26.0 41.0 62-63 34.32876299689425 38.0 34.0 40.0 26.0 41.0 64-65 33.92522046679754 37.0 34.0 40.0 24.5 41.0 66-67 33.49498647942137 36.5 33.0 39.0 22.5 41.0 68-69 33.03872494538102 36.0 33.0 39.0 22.0 40.0 70-71 32.59987570069836 35.5 32.0 38.5 20.5 40.0 72-73 32.12381716576818 35.0 32.0 38.0 19.5 40.0 74-75 31.627383413141104 35.0 31.0 37.0 17.5 39.0 76-77 30.342905937628757 34.0 29.5 36.0 16.0 38.5 78-79 30.945983470616547 34.0 31.0 36.0 15.0 39.0 80-81 30.74279133298017 34.0 31.0 36.0 11.0 37.5 82-83 30.33944356153854 34.0 31.0 35.5 7.0 37.0 84-85 29.90582250475211 34.0 30.0 35.0 4.0 37.0 86-87 29.53955678123669 34.0 30.0 35.0 2.0 36.0 88-89 29.10201631471614 34.0 29.5 35.0 2.0 36.0 90-91 28.56504419723079 34.0 29.0 35.0 2.0 35.5 92-93 28.09407881752932 34.0 28.5 35.0 2.0 35.0 94-95 27.607766455807962 33.0 27.0 35.0 2.0 35.0 96-97 26.940472233474942 33.0 26.0 35.0 2.0 35.0 98-99 26.000621496508195 32.5 24.5 34.0 2.0 35.0 100-101 23.048959729103693 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18330093871298203 1101 2 0.2534157731815725 1101 3 0.257632595067939 1101 4 0.09949538569645 1101 5 0.14376219060758189 1101 6 0.21524265788004016 1101 7 0.16752893589036688 1101 8 0.22718240055135652 1101 9 0.1761079784478241 1101 10-11 0.2273427593345616 1101 12-13 0.20818462131921933 1101 14-15 0.2787426417189991 1101 16-17 0.3709816725244792 1101 18-19 0.29424778945189445 1101 20-21 0.16630997734323927 1101 22-23 0.2616837567394086 1101 24-25 0.22200334827498835 1101 26-27 0.2909411453029378 1101 28-29 -0.03613630244169741 1101 30-31 0.17940616836055057 1101 32-33 0.3378381679996423 1101 34-35 0.3309087404619575 1101 36-37 0.39074519766489857 1101 38-39 0.4730808047637325 1101 40-41 0.5230416453855682 1101 42-43 0.4270679174147247 1101 44-45 0.47803637279019284 1101 46-47 0.4551619904521189 1101 48-49 0.4970366705478 1101 50-51 0.5081870155967678 1101 52-53 0.554740679201096 1101 54-55 0.7808625757725594 1101 56-57 0.8037218436322533 1101 58-59 0.7100992204719105 1101 60-61 0.7379854463228881 1101 62-63 0.8437326578733817 1101 64-65 0.9206315389236934 1101 66-67 0.8453906393510877 1101 68-69 0.9162646773362155 1101 70-71 0.9213658805175058 1101 72-73 0.9012028459293688 1101 74-75 0.8906103553568521 1101 76-77 0.9459135812625661 1101 78-79 0.992107404607907 1101 80-81 0.9537268493489712 1101 82-83 0.9288324741006768 1101 84-85 0.9751912167026475 1101 86-87 1.1090839470831462 1101 88-89 1.3346582138085203 1101 90-91 1.2507156581813241 1101 92-93 1.503640799284291 1101 94-95 1.4614238703671383 1101 96-97 1.6424487776738736 1101 98-99 2.145689014567825 1101 100-101 2.709266367889118 1102 1 0.25219926536565396 1102 2 0.27671061316375756 1102 3 0.24923404572646035 1102 4 0.12388776337667196 1102 5 0.20809539669016885 1102 6 0.16251312934405604 1102 7 0.15509812848437576 1102 8 0.05381121012061385 1102 9 0.1822160143325391 1102 10-11 0.1591999839957552 1102 12-13 0.1524209577701967 1102 14-15 0.2739071133102655 1102 16-17 0.1399897529469527 1102 18-19 0.3251259786737535 1102 20-21 0.2547731064391243 1102 22-23 0.1436149458976388 1102 24-25 0.018604957996942062 1102 26-27 0.16557132281772624 1102 28-29 0.012393951423540273 1102 30-31 0.21915612064152157 1102 32-33 0.3103480299325838 1102 34-35 0.2949285915785893 1102 36-37 0.43371220790858445 1102 38-39 0.5053348926939947 1102 40-41 0.5172875256426224 1102 42-43 0.25264293173042773 1102 44-45 0.3506265617575579 1102 46-47 0.6397948720452931 1102 48-49 0.6564261850861968 1102 50-51 0.6389132992386877 1102 52-53 0.7150805977606112 1102 54-55 0.8687331935749398 1102 56-57 0.6297541653221472 1102 58-59 0.5690934623755766 1102 60-61 0.5822981332224231 1102 62-63 0.7820399048034616 1102 64-65 1.018590755059087 1102 66-67 0.9243218921346923 1102 68-69 0.661075232785727 1102 70-71 0.5732794136345163 1102 72-73 0.7377611903971442 1102 74-75 0.7931243529390919 1102 76-77 0.7626367154484797 1102 78-79 0.8021299916156046 1102 80-81 0.7533427111546622 1102 82-83 0.5143887189311194 1102 84-85 0.8493442039139474 1102 86-87 0.8826412417322516 1102 88-89 0.8930822132995182 1102 90-91 1.2008254119511292 1102 92-93 1.2523762449800344 1102 94-95 1.3135664548298216 1102 96-97 1.6063692141439354 1102 98-99 1.7969145983306305 1102 100-101 2.1556938031502995 1103 1 0.1225341022670321 1103 2 0.13421656548491256 1103 3 0.04625738284382663 1103 4 0.15474439531728024 1103 5 0.05130322060192327 1103 6 0.022967615435220523 1103 7 0.1617988849565819 1103 8 0.13415749958927137 1103 9 0.08994499033237702 1103 10-11 0.09109488157564982 1103 12-13 0.13153634740863396 1103 14-15 0.23870302655204512 1103 16-17 0.22426407207174037 1103 18-19 0.27900302431324064 1103 20-21 0.292088132408324 1103 22-23 0.18942144774110403 1103 24-25 0.15242921131969922 1103 26-27 0.1274224695813757 1103 28-29 0.14446641402689409 1103 30-31 0.08072195783423552 1103 32-33 -0.0229781874049948 1103 34-35 0.3181752939424385 1103 36-37 0.09890167757434654 1103 38-39 0.17571945784805365 1103 40-41 0.1002558502525801 1103 42-43 0.0044236050208326105 1103 44-45 -0.10685327067329808 1103 46-47 -0.39599816800855336 1103 48-49 -0.3910504828194874 1103 50-51 -0.4472459215848943 1103 52-53 -0.257617838059808 1103 54-55 -0.2800811140972783 1103 56-57 -0.21629796395122014 1103 58-59 -0.24805725943754453 1103 60-61 -0.1792825219849803 1103 62-63 -0.2525463460881312 1103 64-65 -0.23330168122345185 1103 66-67 -0.11276584055836025 1103 68-69 0.08296671468766448 1103 70-71 -0.12256450997485757 1103 72-73 -0.05405323782840554 1103 74-75 -0.08261403626736907 1103 76-77 -0.27002444929770775 1103 78-79 -0.31611274253414123 1103 80-81 -0.11811101143200986 1103 82-83 -0.3725611308795145 1103 84-85 -0.28701166954523316 1103 86-87 -0.4517536307323624 1103 88-89 -0.35323086372401846 1103 90-91 -0.2359142229731752 1103 92-93 -0.14364273212487433 1103 94-95 0.003444243881570941 1103 96-97 0.31516383143731375 1103 98-99 0.3908432307534504 1103 100-101 0.8178543701528369 1104 1 0.21498690507637264 1104 2 0.23634243433404123 1104 3 0.18592043625900345 1104 4 0.07885160295589344 1104 5 0.2085884933371105 1104 6 0.11379518062611282 1104 7 0.14904947628187415 1104 8 0.11097754805618365 1104 9 0.1789615764627186 1104 10-11 0.12570228511344084 1104 12-13 0.05980097091943293 1104 14-15 -0.05038611482911648 1104 16-17 -0.09068085849289531 1104 18-19 0.05088206019906494 1104 20-21 0.04134444108738222 1104 22-23 0.032586017561023084 1104 24-25 -0.15568827014243425 1104 26-27 -0.15788364102184715 1104 28-29 -0.1801602892076204 1104 30-31 -0.13178076298766683 1104 32-33 -0.29624302858155005 1104 34-35 -0.22985773320773717 1104 36-37 -0.04069607611508275 1104 38-39 0.0751245047913045 1104 40-41 -0.15184462425876433 1104 42-43 -0.20363583224064286 1104 44-45 -0.2705437209511885 1104 46-47 -0.3583477972512199 1104 48-49 -0.17537854864161062 1104 50-51 -0.23740162515316143 1104 52-53 -0.262680743462262 1104 54-55 -0.18407745731263248 1104 56-57 -0.3593812718902214 1104 58-59 -0.33156399982034657 1104 60-61 -0.10041061102477755 1104 62-63 -0.18610605180403894 1104 64-65 -0.3866196328436118 1104 66-67 -0.30746640503821965 1104 68-69 -0.27672884347988713 1104 70-71 -0.29700329510973233 1104 72-73 -0.09793900684151069 1104 74-75 0.01003756792922772 1104 76-77 0.34670969375222427 1104 78-79 -0.15099295381503097 1104 80-81 -0.06057905084402293 1104 82-83 0.02144000098239829 1104 84-85 0.0252153413235483 1104 86-87 0.02077404909582725 1104 88-89 -0.065727780125858 1104 90-91 0.16420476763817504 1104 92-93 0.433293404743349 1104 94-95 0.6003679012466527 1104 96-97 0.859442849909879 1104 98-99 1.087414269599531 1104 100-101 2.2829291746164415 1105 1 0.11246410775803639 1105 2 0.19552014221063274 1105 3 0.14005254949892532 1105 4 0.14997605883871756 1105 5 0.010227993861370521 1105 6 0.15021687541212003 1105 7 0.04963410437579796 1105 8 0.025154779333782074 1105 9 0.0731280048298899 1105 10-11 0.16405579447066998 1105 12-13 0.11121805858187628 1105 14-15 0.23639144053580452 1105 16-17 0.23384492551245017 1105 18-19 0.3484784126281326 1105 20-21 0.21846451225949437 1105 22-23 0.3282759740316479 1105 24-25 0.22436923946897736 1105 26-27 0.09461214902040638 1105 28-29 0.15643774720454218 1105 30-31 0.12485826093760721 1105 32-33 0.009384016822444607 1105 34-35 0.008897707964059975 1105 36-37 0.10568653755377255 1105 38-39 0.020166286636062125 1105 40-41 0.007124968725705116 1105 42-43 -0.030638342328657586 1105 44-45 -0.02214350489298056 1105 46-47 -0.30756935223485726 1105 48-49 -0.27693702733763814 1105 50-51 -0.005933382532496978 1105 52-53 -0.1412385549197026 1105 54-55 -0.3387385954310176 1105 56-57 -0.2443604604010048 1105 58-59 -0.4352894152558022 1105 60-61 -0.40435526199545535 1105 62-63 -0.256614106326964 1105 64-65 -0.36724270658935865 1105 66-67 -0.2753547537815848 1105 68-69 -0.2562605055394229 1105 70-71 -0.2795724813963929 1105 72-73 -0.4103877724501288 1105 74-75 -0.20772527641354444 1105 76-77 -0.05382409711613789 1105 78-79 -0.1019636270905373 1105 80-81 -0.20455801694194875 1105 82-83 -0.13649060620471332 1105 84-85 -0.34008189757964047 1105 86-87 0.0614263542462723 1105 88-89 0.2876401860673852 1105 90-91 0.473449199373313 1105 92-93 0.44784873788604074 1105 94-95 0.47194671483290307 1105 96-97 0.7482793129099115 1105 98-99 0.8491131861311629 1105 100-101 1.560422581663751 1106 1 0.013211004902302648 1106 2 0.1462487497323437 1106 3 0.16229783460436664 1106 4 0.005781525309160429 1106 5 0.10725656820655871 1106 6 0.0941272817820078 1106 7 0.032692809810789925 1106 8 -0.11135811272341556 1106 9 -0.01339892344927307 1106 10-11 -0.0752150025315288 1106 12-13 -0.03477353669854466 1106 14-15 0.01112496498860338 1106 16-17 -0.12064624017451564 1106 18-19 -0.009621763462654087 1106 20-21 0.16272369747807858 1106 22-23 0.36573354636072253 1106 24-25 0.2040273511292483 1106 26-27 0.23301780434184138 1106 28-29 -0.014461761953029395 1106 30-31 -0.016384412579874663 1106 32-33 0.12993389645121312 1106 34-35 -0.167578009371951 1106 36-37 -0.09047604443052393 1106 38-39 0.07794873370248467 1106 40-41 -0.06609094239524183 1106 42-43 -0.2636800341612755 1106 44-45 -0.4016786932760894 1106 46-47 -0.667239033144746 1106 48-49 -0.8981738311622962 1106 50-51 -0.7988735882269538 1106 52-53 -0.9693990442309115 1106 54-55 -0.8632360561508676 1106 56-57 -0.9250895783307911 1106 58-59 -0.8930340070569471 1106 60-61 -0.8936473260006252 1106 62-63 -0.9402631837643725 1106 64-65 -0.7018556046013273 1106 66-67 -0.848906683549437 1106 68-69 -0.6516608781373208 1106 70-71 -0.6392102618018249 1106 72-73 -0.828837322865688 1106 74-75 -0.8053939875480971 1106 76-77 -1.020596531677569 1106 78-79 -0.6419413392714262 1106 80-81 -0.5267254648165256 1106 82-83 -0.9076980615652737 1106 84-85 -0.5185758534973992 1106 86-87 -0.628434814758343 1106 88-89 -0.44546530677414253 1106 90-91 -0.5342090483957804 1106 92-93 -0.2001866163796926 1106 94-95 -0.7674644443888958 1106 96-97 -0.3889034897408088 1106 98-99 -0.5238611318360533 1106 100-101 0.17966879401052083 1107 1 0.1080684481579155 1107 2 0.11497491296651674 1107 3 0.1903556406942073 1107 4 0.06262383672812888 1107 5 0.03031406506268297 1107 6 0.08177854860947775 1107 7 0.10072422795662561 1107 8 0.19764883472746675 1107 9 0.24919719669833285 1107 10-11 0.0016089610201177607 1107 12-13 0.05751984363831042 1107 14-15 0.09118815174515049 1107 16-17 0.2782034103913773 1107 18-19 0.13831564763265192 1107 20-21 0.1480376727806103 1107 22-23 0.2729995079745109 1107 24-25 0.17407033461616805 1107 26-27 -0.017054350192559298 1107 28-29 0.2348605008131699 1107 30-31 0.162623256416353 1107 32-33 0.238176005837488 1107 34-35 0.15381632546631607 1107 36-37 0.37993801951901673 1107 38-39 0.42714575681254985 1107 40-41 0.4665718691577325 1107 42-43 0.40946765586284783 1107 44-45 0.3779201275126596 1107 46-47 0.40056303665961224 1107 48-49 0.27976698150391854 1107 50-51 0.41257527482374456 1107 52-53 0.7373365815550557 1107 54-55 0.5735282252930531 1107 56-57 0.670221407712468 1107 58-59 0.9355242422678955 1107 60-61 0.9467365361223656 1107 62-63 0.6262813355833501 1107 64-65 0.8730073017833249 1107 66-67 0.8058854083135998 1107 68-69 0.5015051597541245 1107 70-71 0.6672609691545333 1107 72-73 0.6863125524100511 1107 74-75 0.7274492103408683 1107 76-77 0.7690936161361464 1107 78-79 0.9044875266654486 1107 80-81 0.7279748877099159 1107 82-83 0.9495931541355525 1107 84-85 1.1759141170223195 1107 86-87 0.9996317529535332 1107 88-89 0.8877314483333691 1107 90-91 1.1239356523690596 1107 92-93 1.2362040076539529 1107 94-95 1.281062056940808 1107 96-97 1.271789812256845 1107 98-99 1.6828693400546015 1107 100-101 2.4594480011352644 1108 1 0.2295537663291114 1108 2 0.28116127223645293 1108 3 0.3186626220271265 1108 4 0.21804222341452828 1108 5 0.18449923005476165 1108 6 0.20917684027290306 1108 7 0.17697627250838366 1108 8 0.22667497902738631 1108 9 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0.6372014979185892 2316 72-73 0.5524213082246874 2316 74-75 0.8079929894140641 2316 76-77 0.5407770642901824 2316 78-79 0.42482403964902105 2316 80-81 0.4942558745791352 2316 82-83 0.6981284541414396 2316 84-85 0.6638947447088306 2316 86-87 0.9092104492969355 2316 88-89 0.8589747504590228 2316 90-91 1.1491888467399036 2316 92-93 1.1668110853198534 2316 94-95 1.165777654891702 2316 96-97 1.5207299288440161 2316 98-99 2.05327651193236 2316 100-101 2.5629191193122693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 4.0 5 6.0 6 8.0 7 45.0 8 118.0 9 189.0 10 340.0 11 643.0 12 1124.0 13 1729.0 14 2221.0 15 3483.0 16 5200.0 17 8384.0 18 7525.0 19 3909.0 20 2871.0 21 2745.0 22 2904.0 23 3049.0 24 3383.0 25 3799.0 26 4599.0 27 5600.0 28 7003.0 29 8654.0 30 10923.0 31 14515.0 32 18964.0 33 26073.0 34 37751.0 35 55751.0 36 88145.0 37 134656.0 38 103174.0 39 8150.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79401606662209 20.496547997214844 15.605200347798373 20.104235588364688 2 15.58051928716601 21.754628331238596 40.564332678944254 22.10051970265114 3 19.57263891918468 26.778016681728 30.678037109746935 22.97130728934038 4 12.501601348941724 18.233045609880236 38.47825108458931 30.78710195658873 5 12.518826800361474 39.081321390361374 34.13147425532431 14.268377553952849 6 29.528008891381802 38.30876777497325 17.690145038934418 14.473078294710529 7 27.107634885516536 34.89867356371984 20.89613217966962 17.097559371094007 8 26.434548973578607 35.13099899937331 19.53697644545546 18.897475581592623 9 26.8438018274421 17.238304959161272 20.82775025188786 35.09014296150876 10-11 24.40957831721597 28.21126726427278 26.98697107877252 20.39218333973873 12-13 26.03027994667682 25.7324146050103 28.05465625595125 20.18264919236163 14-15 22.79567826216419 26.585162333502986 27.543721154079197 23.07543825025362 16-17 20.680599268053694 30.048490577143472 28.150416004487237 21.120494150315597 18-19 20.45675665382125 27.792146638552172 31.95348990198013 19.797606805646442 20-21 21.676137716128192 26.611153188634738 32.128454264136245 19.584254831100818 22-23 21.265145862511318 26.79002065325215 31.941994311266843 20.00283917296969 24-25 20.970418298673476 26.747397805717256 32.38328536495639 19.898898530652875 26-27 20.858197909038502 26.88074871587126 32.44366193525602 19.81739143983422 28-29 20.356911613477326 27.31656688976528 32.4718414464823 19.854680050275096 30-31 20.643241343611994 27.00761897177639 32.358211125672355 19.990928558939263 32-33 20.730579931687203 27.117982400711867 32.13532027899875 20.016117388602183 34-35 21.02796214930458 27.053362140302404 32.04030898244229 19.878366727950723 36-37 21.134516773480968 26.78199841423175 32.091984945588756 19.99149986669852 38-39 20.701892881008522 27.230982033730466 32.185815337633606 19.881309747627405 40-41 20.80316738164733 27.314252178700155 32.068873585186566 19.813706854465945 42-43 20.765186738910064 27.44983634429248 31.49781547115617 20.287161445641285 44-45 20.976978661376155 27.2804074524183 31.29269542516247 20.449918461043076 46-47 21.250419813100937 27.950463785277286 30.571742163777316 20.227374237844465 48-49 21.341913101284888 28.08151472029195 30.21555022349638 20.361021954926787 50-51 21.241590754070888 28.329680526558153 30.004431841395473 20.42429687797548 52-53 21.392810029811056 28.6827563283579 29.445864018641434 20.47856962318961 54-55 21.02086427831964 28.559668858350733 29.538828816663727 20.880638046665904 56-57 21.336632977747307 28.687430536079688 29.405440777788165 20.570495708384836 58-59 21.486813540660414 28.25316201496439 29.589552626385384 20.670471817989814 60-61 21.188616399890588 28.42169317115564 30.156603270560456 20.23308715839332 62-63 21.08076338468037 28.31124337387776 30.190967353255843 20.41702588818603 64-65 21.02121051592866 28.441774952478887 29.82551355693358 20.71150097465887 66-67 21.03497346088727 28.416499607020313 29.618809704347704 20.929717227744714 68-69 21.069250984180403 28.586848510658925 29.581502601975636 20.76239790318504 70-71 21.21250679491308 28.650296552512128 29.43244731129185 20.704749341282948 72-73 21.280406206666125 28.35855390149869 29.39943251557639 20.961607376258794 74-75 21.124302764014832 28.92096780336474 29.14861903129642 20.806110401324013 76-77 21.13997001582306 28.425501784854873 29.413144564588894 21.02138363473317 78-79 21.18264380113497 28.4291372797496 29.059462846973368 21.32875607214207 80-81 21.15459855480422 28.653932047406855 29.011422378721623 21.180047019067306 82-83 21.19590573672935 28.77903529462682 29.0737703144273 20.95128865421653 84-85 20.882632732925867 28.6576915719342 29.10771585195162 21.351959843188308 86-87 20.66562449146351 28.681717615530832 29.073745148345502 21.57891274466015 88-89 20.989789452909953 28.648132567455743 28.909368843462513 21.452709136171787 90-91 20.734763627486373 28.351023722521894 29.126249732718 21.78796291727373 92-93 20.79953880751273 28.109457042793608 29.29550423885062 21.795499910843045 94-95 20.6260668446328 28.189973651317953 29.37133637329954 21.81262313074971 96-97 20.74439354751592 28.26701151932525 29.295943133935094 21.692651799223736 98-99 20.5682985753151 28.58709586392134 28.68819750829458 22.156408052468983 100-101 20.1996512898294 28.971599339978564 28.791703676000814 22.037045694191224 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 217.0 1 165.5 2 135.5 3 170.0 4 162.0 5 165.0 6 296.0 7 616.5 8 1032.0 9 1298.0 10 1280.0 11 1164.0 12 1214.0 13 1348.0 14 1447.5 15 1507.0 16 1545.5 17 1601.0 18 1627.5 19 1686.0 20 1759.5 21 1912.0 22 2248.0 23 2632.5 24 2984.5 25 3423.5 26 4192.0 27 5240.5 28 6149.0 29 7112.5 30 8547.0 31 9935.5 32 11342.0 33 12906.0 34 14635.0 35 15869.0 36 17567.5 37 19443.5 38 20122.0 39 20555.0 40 20510.0 41 20400.0 42 20539.0 43 20903.5 44 21532.5 45 21838.5 46 21524.0 47 21594.0 48 23283.5 49 22610.0 50 19784.5 51 17720.0 52 16003.0 53 14998.0 54 13816.0 55 12579.5 56 11117.5 57 9628.0 58 8551.0 59 7327.5 60 5984.5 61 4762.0 62 3709.0 63 2804.0 64 1934.0 65 1357.0 66 1000.5 67 741.5 68 530.5 69 345.5 70 262.5 71 196.5 72 154.5 73 132.5 74 95.5 75 77.0 76 57.0 77 30.0 78 21.0 79 14.5 80 7.5 81 5.0 82 4.5 83 1.5 84 1.0 85 1.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050550690917148804 2 0.0 3 0.0 4 0.0 5 6.924752180431343E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004847326526301939 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.655940225539179E-5 22-23 8.655940225539178E-4 24-25 8.655940225539179E-5 26-27 0.001558069240597052 28-29 0.0027699008721725374 30-31 1.7311880451078359E-4 32-33 1.7311880451078359E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.009261856041326922 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.7311880451078359E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.655940225539179E-5 84-85 4.327970112769589E-4 86-87 0.0 88-89 0.0 90-91 0.01116616289094554 92-93 8.655940225539178E-4 94-95 0.0 96-97 0.0 98-99 2.5967820676617536E-4 100-101 0.014022623165373468 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 577638.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.33301045660906 #Duplication Level Percentage of deduplicated Percentage of total 1 84.78838346183485 56.2426872677287 2 9.229023963870054 12.243778861993757 3 2.4983367842388713 4.971666000991442 4 1.089120163524535 2.8897847678230693 5 0.5813343008867541 1.9280827129753284 6 0.3561317503445015 1.4173974671719383 7 0.2690000565994421 1.2490508497067454 8 0.17435396636821884 0.925233877940346 9 0.14432055822933929 0.8615895388317376 >10 0.7910716239365432 9.574077854817624 >50 0.05393098038016899 2.435601219087425 >100 0.02315084527615875 2.7280744360729665 >500 5.261555744577258E-4 0.20417367685049867 >1k 0.0013153889361443144 2.3288014680084657 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4552 0.7880367981330868 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3933 0.6808762581409118 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2477 0.42881527877321085 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1379 0.23873083142037055 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1004 0.1738112797288267 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 638 0.11044979727787992 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 1.7311880451078356E-4 9 0.0 0.0 0.0 0.0 1.7311880451078356E-4 10-11 0.0 0.0 0.0 0.0 1.7311880451078356E-4 12-13 0.0 0.0 0.0 0.0 1.7311880451078356E-4 14-15 0.0 0.0 0.0 0.0 1.7311880451078356E-4 16-17 0.0 0.0 0.0 0.0 1.7311880451078356E-4 18-19 0.0 0.0 0.0 0.0 1.7311880451078356E-4 20-21 0.0 0.0 0.0 1.7311880451078356E-4 1.7311880451078356E-4 22-23 0.0 0.0 0.0 7.79034620298526E-4 1.7311880451078356E-4 24-25 0.0 0.0 0.0 0.0011252722293200934 1.7311880451078356E-4 26-27 0.0 0.0 0.0 0.0017311880451078357 1.7311880451078356E-4 28-29 0.0 0.0 0.0 0.003289257285704888 1.7311880451078356E-4 30-31 0.0 0.0 0.0 0.00969465305260388 1.7311880451078356E-4 32-33 0.0 0.0 0.0 0.01835059327814306 1.7311880451078356E-4 34-35 0.0 0.0 0.0 0.025448464263085183 1.7311880451078356E-4 36-37 0.0 0.0 0.0 0.03220009763900574 1.7311880451078356E-4 38-39 0.0 0.0 0.0 0.03886517161267092 1.7311880451078356E-4 40-41 0.0 0.0 0.0 0.050117893905871846 1.7311880451078356E-4 42-43 0.0 0.0 0.0 0.06448675468026688 1.7311880451078356E-4 44-45 0.0 0.0 0.0 0.07773034322534182 1.7311880451078356E-4 46-47 0.0 0.0 0.0 0.0937438326425893 1.7311880451078356E-4 48-49 0.0 0.0 0.0 0.109584203255326 1.7311880451078356E-4 50-51 0.0 0.0 0.0 0.12940630637181072 1.7311880451078356E-4 52-53 0.0 0.0 0.0 0.18679519006713546 1.7311880451078356E-4 54-55 0.0 0.0 0.0 0.2743067457473366 1.7311880451078356E-4 56-57 0.0 0.0 0.0 0.34926718810050583 1.7311880451078356E-4 58-59 0.0 0.0 0.0 0.4680266879949034 1.7311880451078356E-4 60-61 0.0 0.0 0.0 0.6265169535245257 1.7311880451078356E-4 62-63 0.0 0.0 0.0 0.7342834093324886 1.7311880451078356E-4 64-65 0.0 0.0 0.0 0.8091572922834024 2.596782067661753E-4 66-67 0.0 0.0 0.0 1.0161208230760441 3.462376090215671E-4 68-69 0.0 0.0 0.0 1.2326058881167792 3.462376090215671E-4 70-71 0.0 0.0 0.0 1.3493745217593025 3.462376090215671E-4 72-73 0.0 0.0 0.0 1.594770427153338 3.462376090215671E-4 74-75 0.0 0.0 0.0 1.9406617985658838 3.462376090215671E-4 76-77 0.0 0.0 0.0 2.2958150260197563 3.462376090215671E-4 78-79 0.0 0.0 0.0 2.6261257050263316 3.462376090215671E-4 80-81 0.0 0.0 0.0 2.9838757145478656 3.462376090215671E-4 82-83 0.0 0.0 0.0 3.331065476994242 3.462376090215671E-4 84-85 0.0 0.0 0.0 3.7230930098089114 3.462376090215671E-4 86-87 0.0 0.0 0.0 4.421713945412179 4.327970112769589E-4 88-89 0.0 0.0 0.0 5.20403782299641 5.193564135323507E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACCGT 60 0.003949551 31.666319 6 CGTCGAT 30 0.0039511723 31.666319 44-45 GGTATCA 3895 0.0 30.744677 1 GTATTAC 110 2.7213773E-6 30.240032 1 GTATCAA 5545 0.0 29.137632 1 ACGATGC 70 0.008385081 27.14491 8 CGTCTCA 90 8.93819E-4 26.390884 8 GTACATG 3625 0.0 25.693598 1 GAGTACT 3890 0.0 24.545559 12-13 TACATGG 3770 0.0 24.316711 2 GTACTTT 3970 0.0 23.98903 14-15 ATCAACG 6780 0.0 23.889856 3 CTGGTCG 100 0.0016519253 23.751797 9 ATTCGAT 60 1.7109506E-4 23.74974 84-85 TCAACGC 6860 0.0 23.61126 4 TATCAAC 6875 0.0 23.559742 2 CAACGCA 6905 0.0 23.388594 5 ACATGGG 3750 0.0 23.30641 3 TACCTGG 390 0.0 23.140772 2 AACGCAG 7025 0.0 23.056686 6 >>END_MODULE