FastQCFastQC Report
Thu 26 May 2016
SRR937286_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937286_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564552
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.5850656803979084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25270.4476115574827474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.3050206181184373No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8780.15552154628803017No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7760.13745412291516104No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6870.12168941036432426No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6510.11531267270331166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.032.5036621
ACCGTCA753.0779897E-431.6640058
GTATCAA36350.029.0432661
TACCTGG4200.028.2915152
GTGTAGC2400.027.740461
GTACATG33550.027.3565731
TACATGG33100.026.4213682
GAATAGC1109.393906E-525.9391351
ACATGGG33700.024.5405793
ATCAACG44750.023.7914243
TATCAAC45200.023.449412
TCAACGC45650.023.322374
CAACGCA46150.023.0717415
AACGCAG47050.022.7132876
GGTTAAC1050.002180107222.6452751
TACACCC1954.1846397E-721.9387535
CATGGGG10650.021.4218564
CCTATAC2005.3437543E-721.3883843
CACCGTC1353.7947862E-421.1093377
GAGTACT24050.021.03253612-13
Produced by FastQC (version 0.11.5)