##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937286_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 564552 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89421523615185 33.0 31.0 34.0 30.0 34.0 2 32.01283141322677 33.0 31.0 34.0 30.0 34.0 3 32.04461236520285 34.0 31.0 34.0 30.0 34.0 4 35.65526470546557 37.0 35.0 37.0 35.0 37.0 5 35.66137928835608 37.0 35.0 37.0 35.0 37.0 6 35.727978999277305 37.0 35.0 37.0 35.0 37.0 7 35.67413630631014 37.0 35.0 37.0 35.0 37.0 8 35.677347702248866 37.0 35.0 37.0 35.0 37.0 9 37.31898921622809 39.0 37.0 39.0 35.0 39.0 10-11 37.20719526279244 39.0 37.0 39.0 34.0 39.0 12-13 37.090257407643584 39.0 37.0 39.0 33.5 39.0 14-15 38.279987494508916 40.0 38.0 41.0 34.0 41.0 16-17 38.25105127605606 40.0 38.0 41.0 34.0 41.0 18-19 38.26559909450326 40.0 38.0 41.0 33.5 41.0 20-21 38.225509784749676 40.0 38.0 41.0 34.0 41.0 22-23 38.07756238575011 40.0 38.0 41.0 33.5 41.0 24-25 37.92871072992391 40.0 38.0 41.0 33.0 41.0 26-27 37.76487019796228 40.0 37.5 41.0 33.0 41.0 28-29 37.81980225027987 40.0 38.0 41.0 33.0 41.0 30-31 37.66640451189616 40.0 37.0 41.0 33.0 41.0 32-33 37.45603859343338 40.0 37.0 41.0 33.0 41.0 34-35 37.32722140741686 40.0 36.5 41.0 32.5 41.0 36-37 37.3336743116666 40.0 37.0 41.0 33.0 41.0 38-39 37.241475541668436 40.0 37.0 41.0 32.0 41.0 40-41 37.07449623772478 40.0 36.5 41.0 31.5 41.0 42-43 36.822868398305204 40.0 36.0 41.0 31.0 41.0 44-45 36.56118125522538 40.0 36.0 41.0 30.5 41.0 46-47 36.17376167297255 39.0 36.0 41.0 29.5 41.0 48-49 35.81799019399453 39.0 35.5 41.0 28.0 41.0 50-51 34.95684719919511 38.5 34.5 40.0 26.5 40.5 52-53 35.058423316186996 38.5 35.0 40.0 27.0 40.5 54-55 35.3777481968003 39.0 35.0 40.0 27.0 41.0 56-57 35.208707966670914 39.0 35.0 40.0 26.0 41.0 58-59 34.989212685456785 39.0 35.0 40.0 26.0 41.0 60-61 34.71508470433193 38.0 34.5 40.0 25.5 41.0 62-63 34.402522708271334 38.0 34.0 40.0 24.5 41.0 64-65 34.051328309881114 38.0 34.0 40.0 23.0 41.0 66-67 33.68755313948051 37.0 34.0 40.0 22.0 41.0 68-69 33.22470295030396 36.5 33.0 39.0 21.0 41.0 70-71 32.78832685031671 36.0 33.0 39.0 19.5 40.5 72-73 32.38669334268588 35.5 32.0 39.0 18.0 40.0 74-75 31.936520462242626 35.0 32.0 38.0 15.5 40.0 76-77 31.478279237342175 35.0 31.5 37.0 10.0 39.0 78-79 31.000901599852625 35.0 31.0 37.0 7.5 39.0 80-81 30.503530232821777 34.5 31.0 36.0 4.5 38.5 82-83 30.020188574303162 34.0 30.0 36.0 2.0 37.0 84-85 29.579136200031172 34.0 30.0 35.0 2.0 37.0 86-87 29.150709766327992 34.0 29.0 35.0 2.0 36.5 88-89 28.746955107766865 34.0 29.0 35.0 2.0 36.0 90-91 28.319919157136987 34.0 29.0 35.0 2.0 36.0 92-93 27.873122405022038 34.0 27.5 35.0 2.0 35.0 94-95 27.369204253992546 33.5 26.5 35.0 2.0 35.0 96-97 26.776820381470618 33.0 25.5 35.0 2.0 35.0 98-99 26.043554889540733 33.0 24.5 35.0 2.0 35.0 100-101 24.138450488174694 30.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08356270244953379 1101 2 -0.09685446412021292 1101 3 -0.13273744776950025 1101 4 -0.16270596361666634 1101 5 -0.1684227029021983 1101 6 -0.20149547674697033 1101 7 -0.3731865505064178 1101 8 -0.11577238320036543 1101 9 -0.07055554964987465 1101 10-11 -0.3652126692513349 1101 12-13 -0.39992578333377793 1101 14-15 -0.28868554562745175 1101 16-17 -0.117622060401672 1101 18-19 -0.13622824366722597 1101 20-21 -0.24361058870532304 1101 22-23 -0.20437385567336008 1101 24-25 -0.23579394869915404 1101 26-27 -0.4206949132516229 1101 28-29 -0.46101222800923125 1101 30-31 -0.2937518900569813 1101 32-33 -0.45159889521841734 1101 34-35 -0.3825261152404451 1101 36-37 -0.24516143957714576 1101 38-39 -0.33222212308834287 1101 40-41 -0.578748554134485 1101 42-43 -0.3810889949212566 1101 44-45 -0.2586545704605996 1101 46-47 -0.18309725900649454 1101 48-49 -0.12046409027105653 1101 50-51 -0.247694868112319 1101 52-53 -0.2895340714079069 1101 54-55 -0.17158111895131611 1101 56-57 -0.39511731932464045 1101 58-59 -0.18454900134423013 1101 60-61 -0.5530759775390592 1101 62-63 -0.4204143025044189 1101 64-65 -0.33496138985971413 1101 66-67 -0.2895017591786555 1101 68-69 -0.19613547156094313 1101 70-71 -0.0801912658681232 1101 72-73 -0.1692059359098863 1101 74-75 -0.22748821903173067 1101 76-77 -0.07942154685439462 1101 78-79 0.038202429084353895 1101 80-81 -0.21942655459843152 1101 82-83 0.022252686946199418 1101 84-85 -0.06723571034386211 1101 86-87 0.184041205782993 1101 88-89 0.23405664672874593 1101 90-91 0.5776459188690275 1101 92-93 0.720514353856224 1101 94-95 0.7389668830255225 1101 96-97 0.8264792038769464 1101 98-99 0.7219685966934932 1101 100-101 1.0486979775906704 1102 1 0.1420995515407526 1102 2 0.10460674856410179 1102 3 0.15245911714291083 1102 4 0.012680295719597723 1102 5 0.14137130778662055 1102 6 0.10476693047793617 1102 7 0.12745349386580784 1102 8 0.15512855171879636 1102 9 0.25534376361463273 1102 10-11 0.36142492775416457 1102 12-13 0.2512132136219023 1102 14-15 0.3364678064972324 1102 16-17 0.36748770289941746 1102 18-19 0.3691631704939766 1102 20-21 0.22409263125842216 1102 22-23 0.3095731577401537 1102 24-25 0.192365966818123 1102 26-27 0.27329963736202245 1102 28-29 0.4049991439419429 1102 30-31 0.4578756401551516 1102 32-33 0.3621246902208455 1102 34-35 0.24296331893526002 1102 36-37 0.49633349098940016 1102 38-39 0.486555227645006 1102 40-41 0.3725900551908907 1102 42-43 0.6641966904902858 1102 44-45 0.6853787588512432 1102 46-47 0.9057631486477149 1102 48-49 0.484307334763848 1102 50-51 0.7149914067354288 1102 52-53 1.031696129912298 1102 54-55 1.0073007070788194 1102 56-57 1.0288333447703621 1102 58-59 0.9501274589675219 1102 60-61 1.0220708722988547 1102 62-63 0.8997214777778311 1102 64-65 0.9609788329728701 1102 66-67 1.1067428960199663 1102 68-69 1.2015146176692326 1102 70-71 0.9770711617253909 1102 72-73 0.9013313409950925 1102 74-75 1.2723355141582928 1102 76-77 1.3643101703603726 1102 78-79 1.1762397099063655 1102 80-81 1.1676859130272277 1102 82-83 1.406775207577251 1102 84-85 1.4589175838086241 1102 86-87 1.1585240389625149 1102 88-89 1.1177119346547038 1102 90-91 1.1057953367185114 1102 92-93 1.0220261747002084 1102 94-95 1.0520418066040698 1102 96-97 1.3905854841458734 1102 98-99 1.4644091007695685 1102 100-101 1.9091393183465328 1103 1 0.11550427009035502 1103 2 0.005956602099058017 1103 3 0.08312573102937648 1103 4 -0.03491886507527653 1103 5 -0.14094204824219503 1103 6 0.20129333377141734 1103 7 -0.04087640875472687 1103 8 -0.061081566244943986 1103 9 -0.06778385890140015 1103 10-11 -0.07695683140979526 1103 12-13 -0.1417040138343566 1103 14-15 -0.15180014536722553 1103 16-17 -0.1459544236147039 1103 18-19 0.0019822267840581276 1103 20-21 -0.11671371819926435 1103 22-23 -0.5865821347940781 1103 24-25 -0.38256772805956274 1103 26-27 -0.012680292968120455 1103 28-29 -0.05798973263156171 1103 30-31 -0.049460477333568065 1103 32-33 -0.042010968080163025 1103 34-35 0.00763258502662012 1103 36-37 0.05079572675853683 1103 38-39 0.09451382158056276 1103 40-41 0.04860541422818443 1103 42-43 -0.07487879838629397 1103 44-45 0.003244525615549776 1103 46-47 0.21974462279860774 1103 48-49 0.15843151337203665 1103 50-51 -0.017695039310012817 1103 52-53 0.021414877438225233 1103 54-55 0.27468848318522276 1103 56-57 0.16607901019669669 1103 58-59 0.27333722066493493 1103 60-61 0.2656462028348372 1103 62-63 0.22804663018064986 1103 64-65 0.22362331876180264 1103 66-67 0.12889771353243873 1103 68-69 0.4342812236386706 1103 70-71 0.2798332154028458 1103 72-73 0.37338719563842915 1103 74-75 0.48373207816747765 1103 76-77 0.7065950250828799 1103 78-79 0.5310275335149477 1103 80-81 0.3825422189412535 1103 82-83 0.1687516939995426 1103 84-85 0.1972459030232514 1103 86-87 0.4603380283536964 1103 88-89 0.47175951600218013 1103 90-91 0.5628612855745025 1103 92-93 0.33365035330839277 1103 94-95 0.3610513862098017 1103 96-97 0.2873177302071497 1103 98-99 0.23015013473362345 1103 100-101 0.5995171585808805 1104 1 0.24814954367778697 1104 2 0.16918520158133532 1104 3 0.1455866789897442 1104 4 0.05897785901648689 1104 5 0.12878034754464096 1104 6 0.20013962494342508 1104 7 0.1325838251112046 1104 8 0.3331298057089995 1104 9 0.26601289196890576 1104 10-11 0.26157748975418116 1104 12-13 0.30167610756169694 1104 14-15 0.49879009831749954 1104 16-17 0.4805061226675349 1104 18-19 0.46919748162651587 1104 20-21 0.3085674564860952 1104 22-23 0.3224500440865725 1104 24-25 0.1828466698695692 1104 26-27 0.1001254287135751 1104 28-29 0.15875243464505928 1104 30-31 0.06234997522378194 1104 32-33 -0.17087935154886935 1104 34-35 0.057531049723571925 1104 36-37 0.01992341577041401 1104 38-39 0.202866494971623 1104 40-41 0.36184740298119067 1104 42-43 0.3436173633560955 1104 44-45 0.5116786504035602 1104 46-47 0.4464963857192501 1104 48-49 0.3943314955196726 1104 50-51 0.5116999888074503 1104 52-53 0.39994852913866197 1104 54-55 0.6500901613071548 1104 56-57 0.5325299608349141 1104 58-59 0.65555515336154 1104 60-61 0.7454016254894782 1104 62-63 0.6440535033396273 1104 64-65 0.6194983076040756 1104 66-67 0.8150186186471302 1104 68-69 0.8988465186489307 1104 70-71 1.0213366073514365 1104 72-73 1.091008591693054 1104 74-75 0.9076408526028956 1104 76-77 0.759866470231497 1104 78-79 1.1476726285787713 1104 80-81 1.1941953475675149 1104 82-83 1.0014014306772232 1104 84-85 1.1087673704873282 1104 86-87 1.2042671098497237 1104 88-89 1.1326382984711643 1104 90-91 1.3074743356503724 1104 92-93 1.3030670274135439 1104 94-95 1.2675228670631427 1104 96-97 1.3317143204062063 1104 98-99 1.7440325769366076 1104 100-101 2.065096690849021 1105 1 0.1488900926589487 1105 2 0.16992051797393515 1105 3 0.050964953832838944 1105 4 0.12135582265825917 1105 5 0.2021026832620194 1105 6 0.2052620537457983 1105 7 0.19462855548142244 1105 8 0.2193871003505521 1105 9 0.20724816842859184 1105 10-11 0.14887854811406243 1105 12-13 0.20845478834350217 1105 14-15 0.24702685367194732 1105 16-17 0.174894033735562 1105 18-19 0.34058183178031953 1105 20-21 0.2622576707192792 1105 22-23 0.14799003345616057 1105 24-25 0.3663686976904117 1105 26-27 0.4685423503541841 1105 28-29 0.6528795424831983 1105 30-31 0.41848683770859907 1105 32-33 0.3837750359766332 1105 34-35 0.24926411059510656 1105 36-37 0.1268059221252571 1105 38-39 0.35258737768176474 1105 40-41 0.10158395591708569 1105 42-43 0.005345659818047466 1105 44-45 -0.010886199269684482 1105 46-47 0.18603052009763843 1105 48-49 -0.011597578119371121 1105 50-51 -0.11622578554680274 1105 52-53 -0.21231390445566234 1105 54-55 -0.26426475691298634 1105 56-57 0.020530809065057554 1105 58-59 0.009766974054493005 1105 60-61 0.03614903191565588 1105 62-63 0.04380674547903851 1105 64-65 0.0380929995560777 1105 66-67 0.3612306376276635 1105 68-69 0.2582083080840576 1105 70-71 0.03352303071191898 1105 72-73 0.13375470925004151 1105 74-75 0.16089125399751936 1105 76-77 0.08928959111255708 1105 78-79 0.16244367528926418 1105 80-81 0.44782993112231395 1105 82-83 0.481506578167366 1105 84-85 0.6037234305133801 1105 86-87 0.6233451277451785 1105 88-89 0.8252264744734106 1105 90-91 0.8202115578068039 1105 92-93 0.6894606447570979 1105 94-95 0.589384897472744 1105 96-97 1.1123284059838134 1105 98-99 1.445184297218944 1105 100-101 1.8430036003445167 1106 1 -0.19609906689661472 1106 2 -0.3523371501844643 1106 3 -0.19993966904369387 1106 4 -0.14711896250555156 1106 5 -0.06471503569805037 1106 6 -0.11860984489135262 1106 7 -0.04115956549568267 1106 8 0.015395111382630944 1106 9 -0.031256639317909674 1106 10-11 -0.39510322040018053 1106 12-13 -0.4922474920952311 1106 14-15 -0.43233083590431676 1106 16-17 -0.44463454783831224 1106 18-19 -0.18038137082890415 1106 20-21 -0.17362213373938573 1106 22-23 -0.017639557763267533 1106 24-25 0.11228521311691253 1106 26-27 0.023973281505149657 1106 28-29 0.014140641257121445 1106 30-31 0.09295975162758907 1106 32-33 0.2887511193635106 1106 34-35 0.056678072786944256 1106 36-37 -0.06942866734692643 1106 38-39 0.2854010460355667 1106 40-41 0.032438077728365045 1106 42-43 0.09355531438924913 1106 44-45 0.18681382289968695 1106 46-47 0.24070431102172307 1106 48-49 0.40473372991081646 1106 50-51 0.5081011487304181 1106 52-53 0.19132262314520432 1106 54-55 -0.01716086524979943 1106 56-57 0.18927350538607612 1106 58-59 0.09160387515282054 1106 60-61 -0.2460576091247333 1106 62-63 -0.05542371447895533 1106 64-65 0.5273972196264936 1106 66-67 0.22406425841126065 1106 68-69 0.25536574440349824 1106 70-71 0.19398039601422568 1106 72-73 -0.194909905810853 1106 74-75 -0.17400145002640954 1106 76-77 0.28850953001528623 1106 78-79 0.5517527801353346 1106 80-81 0.39242530260950304 1106 82-83 0.2754348020253623 1106 84-85 0.30711985260308694 1106 86-87 0.4940169346988412 1106 88-89 0.23792989719813562 1106 90-91 0.34112215513971833 1106 92-93 0.6018728597022545 1106 94-95 0.4948231557871985 1106 96-97 0.3154354990538195 1106 98-99 0.34419531802432957 1106 100-101 0.8339085238226289 1107 1 -0.0036133769684703054 1107 2 -0.048115796090989704 1107 3 0.11140627401580616 1107 4 0.07809018469848183 1107 5 -0.02148503466753482 1107 6 0.06113496273069785 1107 7 0.13072992608690015 1107 8 0.08801345637949254 1107 9 -0.06699200369777714 1107 10-11 0.14453638125988988 1107 12-13 0.4660448549439238 1107 14-15 0.22008899490426614 1107 16-17 0.5176767981047519 1107 18-19 0.21685779239491154 1107 20-21 -0.06026575439835824 1107 22-23 0.2605332498213002 1107 24-25 0.06138023574134621 1107 26-27 0.15739889255224426 1107 28-29 0.14738357053585815 1107 30-31 0.2991254955171101 1107 32-33 0.16619038938590336 1107 34-35 0.06572082032074178 1107 36-37 -0.07493956735910956 1107 38-39 0.23421970393350477 1107 40-41 0.27711220155850214 1107 42-43 0.08863930116122276 1107 44-45 0.1804504079684932 1107 46-47 0.2586586499479253 1107 48-49 0.17545640024985687 1107 50-51 0.1992167253958712 1107 52-53 0.13221804485903732 1107 54-55 0.33181518001780574 1107 56-57 0.5244500509206702 1107 58-59 0.43357477722602056 1107 60-61 0.7188136236493108 1107 62-63 0.7005579423978432 1107 64-65 0.7809196988523013 1107 66-67 0.6958094782383171 1107 68-69 0.7959328073490823 1107 70-71 1.0216318579367893 1107 72-73 0.73238040830811 1107 74-75 0.6708361505656626 1107 76-77 0.8133823553996216 1107 78-79 0.7060275335149484 1107 80-81 0.7361621284435138 1107 82-83 0.9978240921895889 1107 84-85 0.8145535953309455 1107 86-87 0.6793991143265465 1107 88-89 0.7196101947352105 1107 90-91 0.9544902448505184 1107 92-93 0.9631752401862208 1107 94-95 1.052906589829707 1107 96-97 1.1238561917456131 1107 98-99 1.1302632569055682 1107 100-101 1.6921076563184343 1108 1 -0.13977895431051834 1108 2 -0.3133244436525757 1108 3 -0.25075226461332534 1108 4 -0.04684696965043855 1108 5 -0.055756863057013106 1108 6 -0.23775895381027823 1108 7 -0.36968904492030674 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58-59 -0.8696866509335592 2316 60-61 -0.8968436130361113 2316 62-63 -1.1615614845333795 2316 64-65 -1.1562426580998562 2316 66-67 -1.0896720273833225 2316 68-69 -0.9636371403828363 2316 70-71 -1.1201688214229577 2316 72-73 -0.9096114193200222 2316 74-75 -0.7998022219791743 2316 76-77 -1.0679365049806684 2316 78-79 -1.082183318692973 2316 80-81 -1.1406694479969914 2316 82-83 -1.043758490129509 2316 84-85 -1.1066118450621296 2316 86-87 -1.543815059474234 2316 88-89 -1.4645815301115555 2316 90-91 -1.6980805426803478 2316 92-93 -1.703318035155025 2316 94-95 -1.7964685746831428 2316 96-97 -1.5970516685218712 2316 98-99 -2.6400673982629534 2316 100-101 -3.4893108518476694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2112.0 3 448.0 4 377.0 5 368.0 6 429.0 7 465.0 8 555.0 9 764.0 10 897.0 11 1240.0 12 1780.0 13 2274.0 14 2614.0 15 3463.0 16 4678.0 17 6674.0 18 5464.0 19 3319.0 20 3200.0 21 3217.0 22 3489.0 23 3783.0 24 4207.0 25 4712.0 26 5453.0 27 6380.0 28 7714.0 29 9378.0 30 11349.0 31 14316.0 32 18568.0 33 24993.0 34 34884.0 35 51065.0 36 78482.0 37 118840.0 38 107380.0 39 15219.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3218539092237 19.959093445355453 15.175763132923201 21.543289512497648 2 14.565308902699602 20.77036068695172 41.61674147304928 23.047588937299405 3 18.801961505194154 26.418157243662492 30.49320026925406 24.28668098188929 4 11.768049321579612 16.971966202426646 39.11303572475262 32.14694875124112 5 12.3662063894109 38.68318312450932 34.6918439628131 14.258766523266678 6 29.249755503609315 37.523406868618025 18.639055880071638 14.587781747701023 7 26.850114913969815 34.11338769932472 21.623525862298457 17.412971524407016 8 25.843763533228735 34.808174247641226 20.38379349269117 18.96426872643887 9 26.392401356596366 16.864991685434337 21.279431521521147 35.46317543644815 10-11 23.85412460116356 27.663251132717683 27.92152407662985 20.56110018948891 12-13 25.04710237211362 25.395966102266502 28.637443523745542 20.919488001874335 14-15 22.149514547598827 26.267939908792986 27.682101222216605 23.900444321391586 16-17 20.44544939841791 29.851126402345628 28.224929050762203 21.47849514847426 18-19 20.329035521283487 28.03279706814088 31.347199914812812 20.290967495762825 20-21 21.455685756725533 26.933984280027758 31.216957977735067 20.393371985511642 22-23 20.882477168747183 26.977347989820437 31.23711138953862 20.90306345189376 24-25 20.670070848990058 27.09013393431496 31.4645821595411 20.775213057153884 26-27 20.492829466133944 27.2643545987154 31.444940432618807 20.797875502531845 28-29 20.150142422333243 27.539332522871874 31.59158066605734 20.718944388737544 30-31 20.51728728124667 27.123549039792692 31.477050867913814 20.88211281104682 32-33 20.27379220933648 27.34487932963769 31.398075982795476 20.98325247823035 34-35 20.712043613270346 27.383425643829103 31.177134094122096 20.727396648778456 36-37 20.645788135706013 27.065337468223017 31.325782279424487 20.963092116646482 38-39 20.34782917328459 27.47318077356498 31.320242415638116 20.858747637512312 40-41 20.421539807508534 27.499997781743033 31.260032067122683 20.81843034362575 42-43 20.441221229090928 27.42095348294487 30.93908140728666 21.198743880677537 44-45 20.506502091938824 27.57996175501023 30.654734703113284 21.258801449937664 46-47 20.69864229301624 27.81551158044192 30.338983050847457 21.146863075694384 48-49 20.748034605496805 28.040959362767587 29.990691053916457 21.22031497781915 50-51 20.675260504022706 28.199216579965107 29.88097836986868 21.244544546143512 52-53 20.8691592352052 28.484443414824224 29.44802592512708 21.198371424843494 54-55 20.608969125591894 28.32402155316234 29.68884219194809 21.378167129297676 56-57 20.786537789268774 28.478279000519933 29.42782410258139 21.3073591076299 58-59 20.994365822279402 28.12111263919081 29.570649039527968 21.313872499001818 60-61 20.794663676015997 28.165026422540613 30.04228671207061 20.99802318937278 62-63 20.671510877290743 28.23792060380787 30.00795931785895 21.08260920104243 64-65 20.472612803168495 28.1920084890728 29.95814021165899 21.37723849609971 66-67 20.477198561235337 28.299017459306274 29.523511238796253 21.70027274066214 68-69 20.50049506531668 28.284376874239214 29.700636309758288 21.514491750685817 70-71 20.53355859233238 28.372518520424595 29.608189536991603 21.485733350251422 72-73 20.653367902312468 28.175670040158934 29.557364452512907 21.6135976050157 74-75 20.432805783994635 28.5336281646749 29.416081322339867 21.617484728990604 76-77 20.494052821416055 28.212829579283145 29.517260349332968 21.775857249967835 78-79 20.53164936589469 28.054313096206723 29.451004018558663 21.963033519339923 80-81 20.58816744040774 28.24412240725755 29.27562928574471 21.892080866590003 82-83 20.57116542697659 28.3340535159255 29.547420696361847 21.54736036073606 84-85 20.411100129356004 28.199964156497696 29.42538607178282 21.96354964236347 86-87 20.231690308293437 28.30314397555514 29.401619010778024 22.063546705373398 88-89 20.550134104392928 27.948189479792067 29.267407619684732 22.23426879613027 90-91 20.301317599041745 27.74765981988377 29.62246572911583 22.328556851958652 92-93 20.409695124223436 27.611389410885813 29.748944619823583 22.229970845067175 94-95 20.043722789705626 27.552632816402433 29.870881136650574 22.532763257241363 96-97 19.938250520124033 27.70085216056639 30.029943174508823 22.330954144800756 98-99 19.92385539089991 27.87822541004697 29.46535517884489 22.73256402020822 100-101 19.890717363589665 27.84196513566289 29.78071611266811 22.48660138807934 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 755.0 1 439.5 2 137.0 3 150.5 4 130.0 5 147.5 6 260.5 7 506.5 8 851.0 9 1033.0 10 970.0 11 915.0 12 954.5 13 1026.5 14 1111.0 15 1153.5 16 1168.5 17 1236.5 18 1325.0 19 1408.0 20 1556.0 21 1742.0 22 2001.5 23 2394.5 24 2816.5 25 3318.0 26 4170.5 27 5190.0 28 5959.5 29 6971.5 30 8314.0 31 9624.5 32 11066.5 33 12665.5 34 14599.5 35 15997.0 36 17583.5 37 19257.5 38 19802.0 39 20175.5 40 20145.0 41 20156.0 42 20522.5 43 20638.0 44 21045.5 45 21653.5 46 21263.0 47 21180.0 48 22765.5 49 22153.5 50 19456.5 51 17352.0 52 15653.5 53 14759.0 54 13887.5 55 12499.0 56 10797.5 57 9194.5 58 8069.0 59 7015.5 60 5792.0 61 4660.5 62 3675.5 63 2710.0 64 1925.5 65 1414.0 66 983.0 67 699.5 68 534.5 69 384.5 70 270.5 71 192.0 72 141.5 73 121.0 74 92.0 75 67.5 76 46.0 77 30.0 78 23.0 79 15.5 80 7.5 81 7.5 82 7.5 83 4.5 84 3.0 85 3.5 86 4.0 87 3.0 88 1.5 89 2.0 90 2.0 91 0.5 92 1.0 93 2.0 94 2.0 95 2.0 96 2.0 97 1.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31989967266080005 2 0.2733140614150689 3 0.2683543765676147 4 0.2750853774320169 5 0.27597103544049084 6 0.20352421034731966 7 0.19254205104224234 8 0.19909592030494977 9 0.19183352463546316 10-11 0.21158369822443282 12-13 0.20396703935155663 14-15 0.19635038047868045 16-17 0.1985645254998654 18-19 0.19254205104224234 20-21 0.18864515580495686 22-23 0.18953081381343084 24-25 0.18235698394479163 26-27 0.1825341155464864 28-29 0.19254205104224234 30-31 0.20051297311850813 32-33 0.1940476696566481 34-35 0.20290424974138788 36-37 0.1867852739871615 38-39 0.1872281029913985 40-41 0.18536822117360313 42-43 0.18961937961427822 44-45 0.19121356402953138 46-47 0.19519902506766426 48-49 0.19759030169054398 50-51 0.19927305190664454 52-53 0.219200357097309 54-55 0.19626181467783305 56-57 0.1804971021269963 58-59 0.18191415494055463 60-61 0.18031997052530147 62-63 0.18802519519902505 64-65 0.17784012810157435 66-67 0.17943431251682748 68-69 0.1753602856778472 70-71 0.18643101078377192 72-73 0.18439399736428175 74-75 0.18882228740665163 76-77 0.18589961597868754 78-79 0.18448256316512918 80-81 0.1870509713897037 82-83 0.18377403675835 84-85 0.175537417279542 86-87 0.1767773384914056 88-89 0.176865904292253 90-91 0.18191415494055463 92-93 0.1790800493134379 94-95 0.17739729909733737 96-97 0.17438606186852584 98-99 0.1804971021269963 100-101 0.17137482463971432 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 564552.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.52445593994855 #Duplication Level Percentage of deduplicated Percentage of total 1 86.039639974409 59.81859158489834 2 8.599113966242845 11.956974401372937 3 2.2377008282186415 4.667247979148199 4 0.9621506450320646 2.675720005124994 5 0.5076023673692958 1.7645389212590088 6 0.33515563899649 1.3980908073862062 7 0.21516435058278474 1.0471429088358322 8 0.16514474856753567 0.9185279036398011 9 0.1284024433861064 0.8034399016001182 >10 0.7530066481534284 9.418492145988463 >50 0.03981735799620333 1.8909479650503977 >100 0.01531435616024151 1.7692135221170822 >500 0.0010209570773473645 0.5309415103478038 >1k 7.657178080105234E-4 1.3401304432308203 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3303 0.5850656803979084 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2527 0.4476115574827474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1722 0.3050206181184373 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 878 0.15552154628803017 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 776 0.13745412291516104 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 687 0.12168941036432426 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 651 0.11531267270331166 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.7713160169479517E-4 0.0 22-23 0.0 0.0 0.0 3.5426320338959033E-4 0.0 24-25 0.0 0.0 0.0 7.970922076265783E-4 1.7713160169479517E-4 26-27 0.0 0.0 0.0 0.0018598818177953493 1.7713160169479517E-4 28-29 0.0 0.0 0.0 0.003808329436438096 1.7713160169479517E-4 30-31 0.0 0.0 0.0 0.008148053677960577 1.7713160169479517E-4 32-33 0.0 0.0 0.0 0.015056186144057588 1.7713160169479517E-4 34-35 0.0 0.0 0.0 0.02054726579659624 1.7713160169479517E-4 36-37 0.0 0.0 0.0 0.027809661466082842 1.7713160169479517E-4 38-39 0.0 0.0 0.0 0.033389306919468884 1.7713160169479517E-4 40-41 0.0 0.0 0.0 0.04251158440675084 1.7713160169479517E-4 42-43 0.0 0.0 0.0 0.05402513851691253 1.7713160169479517E-4 44-45 0.0 0.0 0.0 0.06686717963978517 1.7713160169479517E-4 46-47 0.0 0.0 0.0 0.08210049738553757 1.7713160169479517E-4 48-49 0.0 0.0 0.0 0.09467684110586802 1.7713160169479517E-4 50-51 0.0 0.0 0.0 0.11230143547450014 1.7713160169479517E-4 52-53 0.0 0.0 0.0 0.16570661338548087 1.7713160169479517E-4 54-55 1.7713160169479517E-4 0.0 0.0 0.24081041250407403 1.7713160169479517E-4 56-57 1.7713160169479517E-4 0.0 0.0 0.30254077569471016 1.7713160169479517E-4 58-59 1.7713160169479517E-4 0.0 0.0 0.4052771046776913 1.7713160169479517E-4 60-61 2.6569740254219274E-4 0.0 0.0 0.5465395570292905 1.7713160169479517E-4 62-63 3.5426320338959033E-4 0.0 0.0 0.6349282262749933 1.7713160169479517E-4 64-65 3.5426320338959033E-4 0.0 0.0 0.7044523799402004 1.7713160169479517E-4 66-67 3.5426320338959033E-4 0.0 0.0 0.9155932491603962 1.7713160169479517E-4 68-69 3.5426320338959033E-4 0.0 0.0 1.1042384049653529 1.7713160169479517E-4 70-71 3.5426320338959033E-4 0.0 0.0 1.203343536113591 1.7713160169479517E-4 72-73 3.5426320338959033E-4 0.0 0.0 1.4262636568464906 1.7713160169479517E-4 74-75 3.5426320338959033E-4 0.0 0.0 1.7429749606767846 1.7713160169479517E-4 76-77 3.5426320338959033E-4 0.0 0.0 2.0667715285748702 1.7713160169479517E-4 78-79 3.5426320338959033E-4 0.0 0.0 2.3600128951806036 1.7713160169479517E-4 80-81 3.5426320338959033E-4 0.0 0.0 2.6877063583159746 1.7713160169479517E-4 82-83 3.5426320338959033E-4 0.0 0.0 2.973596763451374 1.7713160169479517E-4 84-85 4.428290042369879E-4 0.0 0.0 3.322191755586731 1.7713160169479517E-4 86-87 7.085264067791807E-4 0.0 0.0 3.9950261446244104 1.7713160169479517E-4 88-89 7.085264067791807E-4 0.0 0.0 4.725605435814593 1.7713160169479517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2575 0.0 32.503662 1 ACCGTCA 75 3.0779897E-4 31.664005 8 GTATCAA 3635 0.0 29.043266 1 TACCTGG 420 0.0 28.291515 2 GTGTAGC 240 0.0 27.74046 1 GTACATG 3355 0.0 27.356573 1 TACATGG 3310 0.0 26.421368 2 GAATAGC 110 9.393906E-5 25.939135 1 ACATGGG 3370 0.0 24.540579 3 ATCAACG 4475 0.0 23.791424 3 TATCAAC 4520 0.0 23.44941 2 TCAACGC 4565 0.0 23.32237 4 CAACGCA 4615 0.0 23.071741 5 AACGCAG 4705 0.0 22.713287 6 GGTTAAC 105 0.0021801072 22.645275 1 TACACCC 195 4.1846397E-7 21.938753 5 CATGGGG 1065 0.0 21.421856 4 CCTATAC 200 5.3437543E-7 21.388384 3 CACCGTC 135 3.7947862E-4 21.109337 7 GAGTACT 2405 0.0 21.032536 12-13 >>END_MODULE