FastQCFastQC Report
Thu 26 May 2016
SRR937286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937286_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences564552
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48040.850940214541796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40640.7198628292876476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24290.4302526605166575No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13780.24408734713542773No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10630.18829089260156726No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6760.11974096274568152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC756.909615E-638.005149
CTGGTCG1203.781679E-935.6298189
ATACGTC1106.9936505E-834.5501255
GGTATCA34700.032.7408641
GTATCAA51650.030.27941
TGGGACG650.00584278429.2295446
TTAGACT2450.027.1465264
CGTCTCA1501.057264E-625.3367588
TATCAAC62450.025.1034722
ATCAACG62700.024.9276143
TCAACGC62600.024.8915444
CAACGCA63650.024.480925
AACGCAG64850.024.0969076
GAGTACT37050.023.27237712-13
GTGTAGC2955.456968E-1222.5594081
TACCTGG5550.022.255262
GTACTTT38350.022.2317914-15
GTACATG37050.021.5547391
ACGCAGA72750.021.4187037
CGCAGAG73000.021.3453528
Produced by FastQC (version 0.11.5)