##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937286_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 564552 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39027228669813 33.0 31.0 34.0 31.0 34.0 2 32.59850111238646 34.0 31.0 34.0 31.0 34.0 3 32.671142782241496 34.0 31.0 34.0 31.0 34.0 4 36.24293599172441 37.0 35.0 37.0 35.0 37.0 5 36.1516618486871 37.0 35.0 37.0 35.0 37.0 6 36.257345647522286 37.0 36.0 37.0 35.0 37.0 7 36.22740686420383 37.0 36.0 37.0 35.0 37.0 8 36.211163896328415 37.0 36.0 37.0 35.0 37.0 9 37.9404678399864 39.0 38.0 39.0 35.0 39.0 10-11 37.87490700590911 39.0 38.0 39.0 35.0 39.0 12-13 37.80594170244726 39.0 38.0 39.0 35.0 39.0 14-15 39.03389678895832 40.0 38.0 41.0 36.0 41.0 16-17 39.02490913148833 40.0 38.0 41.0 36.0 41.0 18-19 39.07339359350423 40.0 38.5 41.0 36.0 41.0 20-21 39.02303419348439 40.0 38.0 41.0 36.0 41.0 22-23 38.895791707406936 40.0 38.0 41.0 35.0 41.0 24-25 38.755716036786694 40.0 38.0 41.0 35.0 41.0 26-27 38.555787597953774 40.0 38.0 41.0 34.5 41.0 28-29 38.548818355085096 40.0 38.0 41.0 34.5 41.0 30-31 38.619186540832374 40.0 38.0 41.0 35.0 41.0 32-33 38.5211016877099 40.0 38.0 41.0 35.0 41.0 34-35 38.387429324490924 40.0 38.0 41.0 35.0 41.0 36-37 38.23330977483031 40.0 38.0 41.0 34.0 41.0 38-39 38.07838161940795 40.0 38.0 41.0 34.0 41.0 40-41 37.906159220054136 40.0 37.0 41.0 34.0 41.0 42-43 37.66046528929133 40.0 37.0 41.0 33.0 41.0 44-45 37.29426784423755 40.0 37.0 41.0 33.0 41.0 46-47 36.82328731454321 40.0 37.0 41.0 31.5 41.0 48-49 36.382271252249566 40.0 36.5 41.0 30.5 41.0 50-51 36.12135640295314 40.0 36.0 41.0 30.0 41.0 52-53 35.89504066941575 39.5 36.0 41.0 29.0 41.0 54-55 35.70616878516062 39.0 35.5 41.0 28.5 41.0 56-57 35.468143767093196 39.0 35.0 40.5 28.0 41.0 58-59 35.2390886933356 39.0 35.0 40.0 27.5 41.0 60-61 34.917521326644845 38.0 35.0 40.0 26.0 41.0 62-63 34.57565644971588 38.0 34.5 40.0 26.0 41.0 64-65 34.25219023225496 37.5 34.0 40.0 26.0 41.0 66-67 33.878045777891145 37.0 34.0 40.0 24.5 41.0 68-69 33.45461445535575 36.5 34.0 39.0 23.0 41.0 70-71 33.06652000170046 36.0 33.0 39.0 22.5 40.0 72-73 32.627566636908554 35.5 33.0 38.5 21.5 40.0 74-75 32.192792692258635 35.0 32.0 37.5 20.0 39.5 76-77 30.909881640663748 34.0 30.5 36.0 19.0 39.0 78-79 31.47335586447307 35.0 32.0 36.0 18.5 39.0 80-81 31.274669118168035 35.0 32.0 36.0 18.0 38.5 82-83 30.930503301733054 35.0 32.0 36.0 14.0 37.0 84-85 30.59902896455951 34.5 31.0 35.0 9.0 37.0 86-87 30.28160204905837 34.0 31.0 35.0 6.5 36.5 88-89 29.945245079284106 34.0 31.0 35.0 2.0 36.0 90-91 29.54060919100455 34.0 30.5 35.0 2.0 36.0 92-93 29.165238100299 34.0 30.0 35.0 2.0 35.0 94-95 28.79766080006802 34.0 29.5 35.0 2.0 35.0 96-97 28.418719621930308 34.0 29.0 35.0 2.0 35.0 98-99 27.8527593560912 34.0 29.0 35.0 2.0 35.0 100-101 26.3008048859981 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05841275562899284 1101 2 -0.022306822672511828 1101 3 0.01572826091292967 1101 4 0.045660548575469306 1101 5 -0.14523865255310398 1101 6 -0.054781349142473346 1101 7 -0.0399028169255331 1101 8 -0.10934580388447301 1101 9 0.03391306609972844 1101 10-11 -0.07743855733259863 1101 12-13 0.0692439018366926 1101 14-15 0.012251062206239283 1101 16-17 0.04278228465690148 1101 18-19 0.02136593084500049 1101 20-21 -0.06144012115675679 1101 22-23 -0.1259628306558369 1101 24-25 -0.02018251065666732 1101 26-27 0.055522434582606195 1101 28-29 0.14364154178027633 1101 30-31 0.27033688613425255 1101 32-33 0.24741955684803685 1101 34-35 0.29504879016251095 1101 36-37 0.29414044441987386 1101 38-39 0.31296929915357197 1101 40-41 0.3692228627533112 1101 42-43 0.3360092457179249 1101 44-45 0.38659342066109303 1101 46-47 0.3713413750446364 1101 48-49 0.5592768234239003 1101 50-51 0.5508105703418593 1101 52-53 0.45787119015353994 1101 54-55 0.4692054809596442 1101 56-57 0.6135249349198588 1101 58-59 0.5653462274462626 1101 60-61 0.5848886596263654 1101 62-63 0.46585933044702443 1101 64-65 0.6948470269787208 1101 66-67 0.6207088149429438 1101 68-69 0.5772084676201032 1101 70-71 0.5993161788927637 1101 72-73 0.5138518095213485 1101 74-75 0.6111152682554248 1101 76-77 0.5895030723592996 1101 78-79 0.7796035504434471 1101 80-81 0.5968006558475523 1101 82-83 0.4912581790417363 1101 84-85 0.5031046329236588 1101 86-87 0.321293948493782 1101 88-89 0.3761021990301181 1101 90-91 0.5598351587764832 1101 92-93 0.6018578496643627 1101 94-95 0.28388761012848107 1101 96-97 0.019709955098424103 1101 98-99 0.11765439305528957 1101 100-101 0.3743943476118936 1102 1 0.10036995361537748 1102 2 0.05755065973605866 1102 3 -0.031158710541248524 1102 4 0.09160899719251603 1102 5 -0.021996032648587516 1102 6 0.03173510164305071 1102 7 0.0651663031662224 1102 8 -0.026710356550644576 1102 9 0.06065391628547445 1102 10-11 0.13449212045637893 1102 12-13 0.07287813175441471 1102 14-15 0.0734869354006733 1102 16-17 0.009857211369073582 1102 18-19 0.07270187658302518 1102 20-21 0.06477396545997038 1102 22-23 0.0374098663203597 1102 24-25 0.045870538602464705 1102 26-27 2.9216745613780404E-4 1102 28-29 0.03638592983675437 1102 30-31 -0.1381281511481376 1102 32-33 0.03157888950973131 1102 34-35 0.1533694453423351 1102 36-37 0.03594952161220277 1102 38-39 0.004011968256115495 1102 40-41 -0.23381801391162327 1102 42-43 -0.22105889668059575 1102 44-45 -0.13950111788806652 1102 46-47 -0.23058236174906455 1102 48-49 -0.06599012286721262 1102 50-51 -0.25166405898861655 1102 52-53 -0.3038818890317714 1102 54-55 -0.23021730399328533 1102 56-57 -0.12313871823719325 1102 58-59 0.03829201404277427 1102 60-61 0.01643446471130261 1102 62-63 -0.09680077857203884 1102 64-65 -0.03774525457186684 1102 66-67 -0.11685654087315811 1102 68-69 0.12089600062116546 1102 70-71 -0.15259886207164186 1102 72-73 -0.144938733803464 1102 74-75 -0.12060586354984792 1102 76-77 -0.1595885660132801 1102 78-79 0.010548324876769044 1102 80-81 0.10638800349427413 1102 82-83 -0.23329085211753764 1102 84-85 -0.15764364710398127 1102 86-87 -0.16556124793011406 1102 88-89 -0.15846489751865178 1102 90-91 -0.3095809045354194 1102 92-93 -0.3083369525799462 1102 94-95 -0.5547742035864864 1102 96-97 -0.14405992975625992 1102 98-99 -0.09124214537792597 1102 100-101 0.1720071466191584 1103 1 0.04301503318152555 1103 2 0.019944769466086143 1103 3 -0.020260919392491417 1103 4 -0.035817572358588734 1103 5 -0.07722523065349662 1103 6 -0.08951222635948142 1103 7 -0.17003874789861584 1103 8 -0.013690801487705073 1103 9 -0.08508474256972676 1103 10-11 -0.0678018388500945 1103 12-13 -0.01955120875702221 1103 14-15 -0.014432667164435031 1103 16-17 -0.1608299867941767 1103 18-19 -0.1560804842751864 1103 20-21 -0.06585702179361164 1103 22-23 -0.014888051500868471 1103 24-25 0.04073733458061213 1103 26-27 -0.1253530704506005 1103 28-29 0.14313222862544706 1103 30-31 0.006174556475961879 1103 32-33 0.017745859662532837 1103 34-35 0.09766126705082456 1103 36-37 0.28182276996941624 1103 38-39 0.17460218280761808 1103 40-41 -0.07037687702618456 1103 42-43 -0.06374850953784517 1103 44-45 9.69747443257063E-4 1103 46-47 0.08011354514667346 1103 48-49 0.10914842514957002 1103 50-51 0.03932834066302604 1103 52-53 0.12103201327208524 1103 54-55 0.10346470851562373 1103 56-57 -0.006049809995566591 1103 58-59 -0.015398311347134097 1103 60-61 -0.04526096716265471 1103 62-63 0.12735012001081003 1103 64-65 0.1680331873567269 1103 66-67 0.18185892108552082 1103 68-69 0.0715709273886489 1103 70-71 0.24292108884277042 1103 72-73 0.3587723737889661 1103 74-75 0.3934528016389791 1103 76-77 -0.04245611410923189 1103 78-79 0.3533289835736255 1103 80-81 0.2883332176499245 1103 82-83 0.25123729292244334 1103 84-85 0.3270962784759419 1103 86-87 0.24206878927992292 1103 88-89 0.3066635562854785 1103 90-91 0.37451279350907285 1103 92-93 0.3984707031876127 1103 94-95 0.4861885072044245 1103 96-97 0.6351143240637072 1103 98-99 0.3000946450583051 1103 100-101 0.49642920346343544 1104 1 0.10289631498103802 1104 2 0.09180052238941272 1104 3 0.08297482103418474 1104 4 -0.0037882052247866227 1104 5 -0.059033674820234694 1104 6 0.04145046220607895 1104 7 -0.002996437803105323 1104 8 0.018010528513187296 1104 9 0.13429622681347553 1104 10-11 0.16421413901297655 1104 12-13 0.09315744071187027 1104 14-15 -0.07150108633787511 1104 16-17 -0.017845964657247748 1104 18-19 0.11909134469920701 1104 20-21 -0.020110346795839007 1104 22-23 -0.01431008131431355 1104 24-25 -0.010508983369938107 1104 26-27 -0.13210795753441573 1104 28-29 -0.029951989887450736 1104 30-31 -0.12927465355706858 1104 32-33 -0.09696225423553528 1104 34-35 -0.246492084729951 1104 36-37 -0.36502405677342153 1104 38-39 -0.35815020494566596 1104 40-41 -0.24542150792862572 1104 42-43 -0.3260744490183427 1104 44-45 -0.4300520770680336 1104 46-47 -0.3200649784630798 1104 48-49 -0.4734879222573767 1104 50-51 -0.566029599174513 1104 52-53 -0.5376877492915213 1104 54-55 -0.5981408881995378 1104 56-57 -0.38870177821836904 1104 58-59 -0.40077611193626694 1104 60-61 -0.3231262710991061 1104 62-63 -0.22771102432552226 1104 64-65 -0.32165149531756043 1104 66-67 -0.5599490767721989 1104 68-69 -0.5676038472252927 1104 70-71 -0.3734818835753302 1104 72-73 -0.38414247044918426 1104 74-75 -0.5084462432597086 1104 76-77 -0.42079227406638964 1104 78-79 -0.4885091401432362 1104 80-81 -0.5218917220983599 1104 82-83 -0.5067270916174103 1104 84-85 -0.4373641339135972 1104 86-87 -0.3068087435237885 1104 88-89 -0.5362345513642559 1104 90-91 -0.37597703064517063 1104 92-93 -0.5098964752467374 1104 94-95 -0.7728224719975749 1104 96-97 -0.5138859437217107 1104 98-99 -0.6919320442213568 1104 100-101 -0.6031936654109771 1105 1 0.048422314061205896 1105 2 0.11229023759889145 1105 3 -0.032863673742866695 1105 4 -0.015434845765227578 1105 5 0.006387452169178687 1105 6 -0.01637514938713025 1105 7 0.07431643519095132 1105 8 -0.06105388465200434 1105 9 0.041852910894562 1105 10-11 0.006125245595299589 1105 12-13 0.021238264927191608 1105 14-15 0.12773798512551338 1105 16-17 0.08761134981305929 1105 18-19 0.08479172667816925 1105 20-21 0.22201529591750813 1105 22-23 0.07284281216946198 1105 24-25 -0.06871740012633865 1105 26-27 0.2686284604557798 1105 28-29 -0.024404069015204755 1105 30-31 -0.017865958096471957 1105 32-33 0.12379101819781368 1105 34-35 0.24200310541957037 1105 36-37 0.3848306700441526 1105 38-39 0.4434369217848797 1105 40-41 0.27490709863304374 1105 42-43 0.08130460245104842 1105 44-45 0.25677336653154725 1105 46-47 0.5643347472391156 1105 48-49 0.7064607983747209 1105 50-51 0.7175684388801713 1105 52-53 0.5661598556976202 1105 54-55 0.8295736548164712 1105 56-57 0.9245416280315482 1105 58-59 0.9260196986880942 1105 60-61 0.6621880560037781 1105 62-63 0.5786418889870006 1105 64-65 0.5961651933351604 1105 66-67 0.4613213957305504 1105 68-69 0.5246962610066674 1105 70-71 0.5189099326561575 1105 72-73 0.6888273540387857 1105 74-75 0.7083054759210299 1105 76-77 0.731484315056985 1105 78-79 0.5977762452369149 1105 80-81 0.42604539871109637 1105 82-83 0.5068927856095371 1105 84-85 0.3777795281823586 1105 86-87 0.38206291760275946 1105 88-89 0.3642980710714312 1105 90-91 0.4917982103981551 1105 92-93 0.34742047365841344 1105 94-95 0.4029388007882808 1105 96-97 0.6067968264657573 1105 98-99 0.43139869117765883 1105 100-101 0.5141856889977561 1106 1 -0.051437530452624 1106 2 0.034193947398129865 1106 3 -0.06942058325803657 1106 4 0.03360454431416571 1106 5 -0.016623598696362762 1106 6 -0.08330409093139934 1106 7 -0.08693376677573639 1106 8 -0.10995800402089628 1106 9 -0.15166311304835034 1106 10-11 -0.2596572766773946 1106 12-13 -0.42602118805309175 1106 14-15 -0.29189430399308236 1106 16-17 -0.15712154689647662 1106 18-19 -0.25293226846712 1106 20-21 -0.2509136531418008 1106 22-23 -0.2970004616823303 1106 24-25 -0.060214132962926215 1106 26-27 0.22637327226039616 1106 28-29 -0.04954375481140971 1106 30-31 -0.11804143426816438 1106 32-33 -0.14657943567299725 1106 34-35 -0.2608638821392901 1106 36-37 -0.17520134402400345 1106 38-39 0.08439088089554758 1106 40-41 -0.08559122363439542 1106 42-43 -0.13295293043663747 1106 44-45 0.21628609137213317 1106 46-47 -0.2200813154062473 1106 48-49 -0.35975609317949875 1106 50-51 -0.12752405246813936 1106 52-53 -0.32883249774971546 1106 54-55 -0.26871742520742714 1106 56-57 -0.4245676519149413 1106 58-59 -0.5202195876837621 1106 60-61 -0.2303450007760972 1106 62-63 -0.20978908349668046 1106 64-65 -0.19914955530556 1106 66-67 -0.18753274862584135 1106 68-69 -0.4688595926454511 1106 70-71 -0.43240012659993 1106 72-73 -0.3713305369405404 1106 74-75 -0.42417903370385446 1106 76-77 -0.6616255210024242 1106 78-79 -0.22588335353513145 1106 80-81 -0.23738228594281807 1106 82-83 -0.5273006439914489 1106 84-85 -0.35939136005773165 1106 86-87 -0.2655155488031369 1106 88-89 -0.4411488295384167 1106 90-91 -0.2617723727313326 1106 92-93 -0.32798618391505485 1106 94-95 -0.48414397361399253 1106 96-97 -0.3133389702975151 1106 98-99 -0.2037386273811066 1106 100-101 -0.10091277680693622 1107 1 -0.13368179939766378 1107 2 -0.115801836868755 1107 3 -0.09333784246940979 1107 4 -0.0526727759785004 1107 5 0.05444897749129751 1107 6 0.14227510395726028 1107 7 0.01152233807422931 1107 8 0.015947207562064136 1107 9 0.07815055154792105 1107 10-11 0.017831645312803346 1107 12-13 0.031240646446597964 1107 14-15 -0.011548051779826096 1107 16-17 -0.1225946926765289 1107 18-19 -0.1685239231892055 1107 20-21 0.06762714110232082 1107 22-23 -0.04961655828819289 1107 24-25 -0.10422872876780076 1107 26-27 -0.0901720749754844 1107 28-29 -0.20726369897636232 1107 30-31 -0.25881413130684194 1107 32-33 -0.023091244409869205 1107 34-35 0.1096522172770733 1107 36-37 -0.09996456034731693 1107 38-39 -0.2585426135724731 1107 40-41 -0.1413044788361404 1107 42-43 -0.05973267243377478 1107 44-45 -0.1411682616065164 1107 46-47 -0.34929821955921625 1107 48-49 -0.41200542100427384 1107 50-51 -0.3284318403324491 1107 52-53 -0.10843631251977115 1107 54-55 -0.36084524623550607 1107 56-57 -0.11102718556117708 1107 58-59 -0.41681233849646304 1107 60-61 -0.36980847847487297 1107 62-63 -0.16224264017018442 1107 64-65 -0.2734260886613811 1107 66-67 -0.44178361285112544 1107 68-69 -0.3660987558692739 1107 70-71 -0.27263320979976413 1107 72-73 -0.20740409679926586 1107 74-75 -0.1351671078632819 1107 76-77 0.11365248317583365 1107 78-79 0.00361178900348591 1107 80-81 -0.2519382755627433 1107 82-83 -0.41765410979248685 1107 84-85 -0.4953019115693067 1107 86-87 -0.27161899352550734 1107 88-89 -0.11036133059235098 1107 90-91 -0.11038539653617363 1107 92-93 0.15836889323285774 1107 94-95 -0.1705988231123179 1107 96-97 0.1000464507605372 1107 98-99 -0.26427187077880276 1107 100-101 0.16373689577112316 1108 1 0.15641372599198178 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0.1725042275578943 2316 62-63 0.3350656161815806 2316 64-65 0.09033846680922863 2316 66-67 0.06648903351830882 2316 68-69 0.20790382619913572 2316 70-71 0.22618856480822558 2316 72-73 0.299439637924543 2316 74-75 0.5661715567310424 2316 76-77 0.6606561557294484 2316 78-79 0.4725016249293361 2316 80-81 0.38788511597157793 2316 82-83 0.4853052414314867 2316 84-85 0.25861819472166303 2316 86-87 0.4614878865587215 2316 88-89 0.3128127334078492 2316 90-91 0.3366257966050519 2316 92-93 0.6197005386120829 2316 94-95 0.5739675523404841 2316 96-97 0.22428655805425635 2316 98-99 -0.3100283792206575 2316 100-101 -0.12503486692926913 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 2.0 5 10.0 6 24.0 7 40.0 8 86.0 9 186.0 10 398.0 11 656.0 12 1070.0 13 1732.0 14 2235.0 15 3528.0 16 5196.0 17 8210.0 18 7460.0 19 3699.0 20 2622.0 21 2420.0 22 2457.0 23 2499.0 24 2723.0 25 3167.0 26 3743.0 27 4496.0 28 5598.0 29 7083.0 30 9101.0 31 11814.0 32 15339.0 33 21225.0 34 31287.0 35 47109.0 36 77530.0 37 134392.0 38 130542.0 39 14871.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90414212089634 20.52894805866882 15.538270134620703 20.028639685814138 2 15.544892233133528 21.75601184656152 40.53798410066744 22.16111181963752 3 19.56471680199521 26.93498561691394 30.795214612648614 22.705082968442234 4 12.554025138516915 18.111352010089416 38.46519009763494 30.86943275375873 5 12.52829988130879 38.86552463285444 34.23568176584173 14.37049371999504 6 29.504633762700337 38.42055293400785 17.652226898496508 14.422586404795307 7 26.936225538125807 34.93761424988309 20.930578582663774 17.195581629327325 8 26.413864444727853 35.04885289574742 19.535135824512178 19.00214683501254 9 26.942602275786818 17.178222732361235 20.912334027689212 34.966840964162735 10-11 24.427067834318187 28.188191698904618 26.969880542447815 20.414859924329377 12-13 26.048643525858477 25.738731477462956 28.045401805089327 20.16722319158924 14-15 22.82748090521334 26.524040300982016 27.668221882129547 22.9802569116751 16-17 20.686225538125804 29.976866612818657 28.175349657781744 21.161558191273787 18-19 20.298218764613356 27.70834218991342 32.12990123141889 19.86353781405433 20-21 21.622277487281952 26.596309994473494 32.21279882101206 19.568613697232497 22-23 21.16061812719646 26.66948900351434 32.1410731493028 20.028819719986394 24-25 20.954192760435074 26.701419979257835 32.55643206409347 19.787955196213623 26-27 20.747654942931263 26.823059897597773 32.57315488669063 19.856130272780334 28-29 20.35513680977357 27.172775147331556 32.63604366717858 19.836044375716288 30-31 20.697158880060794 26.813196740404056 32.477014818036004 20.012629561499146 32-33 20.61085604160467 27.032850826850318 32.247959443948474 20.108333687596534 34-35 20.881335997392622 26.93985673596055 32.323683203673 19.85512406297383 36-37 21.07311828337918 26.68802287127292 32.27449778673672 19.96436105861118 38-39 20.74418431638594 27.191166077112612 32.240399893012324 19.824249713489124 40-41 20.78444501126557 27.19404058439258 32.208990491575626 19.812523912766228 42-43 20.74775279107423 27.32453700587091 31.644457357062635 20.283252845992227 44-45 20.974595785684933 27.295005597358614 31.373372160580427 20.35702645637603 46-47 21.235776332383907 27.750853774320166 30.723210616559683 20.290159276736244 48-49 21.301315025010982 27.996003911065763 30.311468208420127 20.391212855503124 50-51 21.36942212586263 28.22769204608256 30.067823690288936 20.335062137765874 52-53 21.437548213050537 28.610587342341663 29.560722095326998 20.3911423492808 54-55 20.996914367498476 28.514025280222192 29.686016522835807 20.803043829443524 56-57 21.36570236222704 28.50764854256118 29.59638793237824 20.530261162833536 58-59 21.56408975612521 28.23141180971815 29.65687837435701 20.54762005979963 60-61 21.226919752299167 28.356732417917215 30.260100043928638 20.156247785854976 62-63 21.215051934985617 28.244253850841023 30.23609871189899 20.30459550227437 64-65 21.00913644801542 28.356732417917215 30.015304170386433 20.618826963680938 66-67 21.08282319432045 28.447069534781562 29.643859201632445 20.82624806926554 68-69 21.102219104706034 28.438921481103602 29.72808527823832 20.730774135952046 70-71 21.255349374371182 28.492769488018823 29.572032337145203 20.679848800464793 72-73 21.361008374782127 28.318294860349447 29.449014439768167 20.871682325100256 74-75 21.09380535362553 28.820285819552495 29.211303830293755 20.87460499652822 76-77 21.14145375448143 28.40730349020108 29.454682651022402 20.996560104295085 78-79 21.16324094148989 28.34991285125197 29.13026612251839 21.356580084739758 80-81 21.2191259618246 28.689651263302586 29.02575847751846 21.065464297354364 82-83 21.208275285471487 28.712679247221452 29.17880394687082 20.900241520436243 84-85 20.937789499974315 28.625226064607094 29.124472368306847 21.31251206711174 86-87 20.742641953265597 28.73836245376865 29.028061188340487 21.49093440462526 88-89 20.96237370516799 28.69505377715427 28.93542136065411 21.40715115702362 90-91 20.720277056890552 28.31498392396879 29.218696025721652 21.746042993419014 92-93 20.87133272811815 28.127538028917478 29.293625312756017 21.707503930208357 94-95 20.618915529481786 28.248947838285932 29.30730915841233 21.824827473819948 96-97 20.799324065807934 28.176678144794458 29.325996542391135 21.698001247006477 98-99 20.640854638176364 28.484278434060823 28.783989882081613 22.090877045681196 100-101 20.04852292083536 29.215085508582746 28.627283203611142 22.10910836697075 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 285.0 1 197.5 2 137.0 3 174.5 4 157.0 5 158.0 6 293.5 7 606.5 8 1037.5 9 1370.5 10 1387.5 11 1275.5 12 1287.5 13 1376.0 14 1449.5 15 1503.0 16 1544.5 17 1604.5 18 1704.0 19 1779.5 20 1909.5 21 2048.0 22 2191.0 23 2483.5 24 2852.5 25 3375.5 26 4122.5 27 4988.5 28 5824.0 29 6902.5 30 8306.0 31 9467.5 32 10817.5 33 12415.5 34 14125.5 35 15560.5 36 17209.0 37 18823.5 38 19557.5 39 20044.0 40 20099.5 41 20125.5 42 20218.0 43 20344.5 44 20783.5 45 21240.0 46 20899.5 47 21101.5 48 22923.5 49 22032.0 50 19207.5 51 17205.0 52 15618.5 53 14657.5 54 13570.0 55 12420.5 56 10837.0 57 9304.0 58 8322.0 59 7322.0 60 6005.0 61 4739.0 62 3702.0 63 2715.0 64 1910.5 65 1381.5 66 1018.5 67 739.0 68 499.0 69 321.0 70 252.5 71 211.0 72 173.0 73 140.5 74 102.5 75 75.0 76 50.0 77 30.5 78 17.0 79 10.0 80 7.0 81 3.5 82 2.5 83 2.0 84 1.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.053139480508438544 2 0.0 3 0.0 4 0.0 5 0.0109821593050773 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00655386926270742 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014170528135583613 24-25 2.6569740254219274E-4 26-27 0.0023912766228797347 28-29 0.0015941844152531565 30-31 6.199606059317831E-4 32-33 0.0 34-35 0.0 36-37 1.7713160169479517E-4 38-39 1.7713160169479517E-4 40-41 0.0 42-43 0.01310773852541484 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.856580084739758E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.199606059317831E-4 84-85 1.7713160169479517E-4 86-87 0.0 88-89 0.0 90-91 0.008325185279655372 92-93 0.00256840822457453 94-95 0.0 96-97 0.0 98-99 0.0015941844152531565 100-101 0.013550567529651832 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 564552.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.35730875874867 #Duplication Level Percentage of deduplicated Percentage of total 1 84.81733128282852 56.28249840027726 2 9.299230532555043 12.34143823335076 3 2.494881528514842 4.966608717124747 4 1.01538624924324 2.6951319540168566 5 0.5624976521110511 1.8662915188602112 6 0.36249415952064373 1.443248211993267 7 0.24834984507075666 1.1535879144683319 8 0.17366182955954373 0.9218985314953465 9 0.13784502894984113 0.8232322632194934 >10 0.8094488736206851 9.71002415263012 >50 0.053030671915938486 2.3630703067438463 >100 0.023958008372670446 2.7680190680926064 >500 5.383822106215293E-4 0.210959635997442 >1k 0.0013459555265538234 2.45399109172977 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4804 0.850940214541796 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4064 0.7198628292876476 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2429 0.4302526605166575 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1378 0.24408734713542773 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1063 0.18829089260156726 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 676 0.11974096274568152 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.7713160169479517E-4 0.0 0.0 0.0 0.0 7 1.7713160169479517E-4 0.0 0.0 0.0 0.0 8 1.7713160169479517E-4 0.0 0.0 1.7713160169479517E-4 0.0 9 1.7713160169479517E-4 0.0 0.0 1.7713160169479517E-4 0.0 10-11 1.7713160169479517E-4 0.0 0.0 1.7713160169479517E-4 0.0 12-13 1.7713160169479517E-4 0.0 0.0 1.7713160169479517E-4 0.0 14-15 1.7713160169479517E-4 0.0 0.0 1.7713160169479517E-4 0.0 16-17 1.7713160169479517E-4 0.0 0.0 1.7713160169479517E-4 0.0 18-19 1.7713160169479517E-4 0.0 0.0 1.7713160169479517E-4 0.0 20-21 1.7713160169479517E-4 0.0 0.0 3.5426320338959033E-4 0.0 22-23 1.7713160169479517E-4 0.0 0.0 5.313948050843855E-4 0.0 24-25 1.7713160169479517E-4 0.0 0.0 9.742238093213733E-4 0.0 26-27 1.7713160169479517E-4 0.0 0.0 0.0020370134194901443 0.0 28-29 2.6569740254219274E-4 0.0 0.0 0.003985461038132891 0.0 30-31 3.5426320338959033E-4 0.0 0.0 0.008590882682197565 0.0 32-33 3.5426320338959033E-4 0.0 0.0 0.015587580949141974 0.0 34-35 3.5426320338959033E-4 0.0 0.0 0.02161005540676501 0.0 36-37 3.5426320338959033E-4 0.0 0.0 0.029403845881336 0.0 38-39 3.5426320338959033E-4 0.0 0.0 0.03507205713556944 0.0 40-41 3.5426320338959033E-4 0.0 0.0 0.0441943346228514 0.0 42-43 3.5426320338959033E-4 0.0 0.0 0.05615071773725007 0.0 44-45 3.5426320338959033E-4 0.0 0.0 0.0695241536652071 0.0 46-47 3.5426320338959033E-4 0.0 0.0 0.08458033980926469 0.0 48-49 3.5426320338959033E-4 0.0 0.0 0.09768807833467953 1.7713160169479517E-4 50-51 3.5426320338959033E-4 0.0 0.0 0.11602119911009084 1.7713160169479517E-4 52-53 3.5426320338959033E-4 0.0 0.0 0.16986920602530858 1.7713160169479517E-4 54-55 3.5426320338959033E-4 0.0 0.0 0.2447958735422069 1.7713160169479517E-4 56-57 3.5426320338959033E-4 0.0 0.0 0.3072347631396222 1.7713160169479517E-4 58-59 3.5426320338959033E-4 0.0 0.0 0.4114767107370092 1.7713160169479517E-4 60-61 3.5426320338959033E-4 0.0 0.0 0.552916294690303 1.7713160169479517E-4 62-63 3.5426320338959033E-4 0.0 0.0 0.6421906219444798 1.7713160169479517E-4 64-65 3.5426320338959033E-4 0.0 0.0 0.7123347362156187 1.7713160169479517E-4 66-67 5.313948050843855E-4 0.0 0.0 0.9249812240502203 1.7713160169479517E-4 68-69 5.313948050843855E-4 0.0 0.0 1.1153091300712776 1.7713160169479517E-4 70-71 6.199606059317831E-4 0.0 0.0 1.214502827020363 1.7713160169479517E-4 72-73 7.085264067791807E-4 0.0 0.0 1.4393713953719054 1.7713160169479517E-4 74-75 7.085264067791807E-4 0.0 0.0 1.7574111862149102 1.7713160169479517E-4 76-77 7.970922076265783E-4 0.0 0.0 2.0844846887443493 1.7713160169479517E-4 78-79 8.856580084739759E-4 0.0 0.0 2.3813572531848264 1.7713160169479517E-4 80-81 8.856580084739759E-4 0.0 0.0 2.7142760985701937 1.7713160169479517E-4 82-83 8.856580084739759E-4 0.0 0.0 3.005657583358132 1.7713160169479517E-4 84-85 8.856580084739759E-4 0.0 0.0 3.3586808655358587 1.7713160169479517E-4 86-87 8.856580084739759E-4 0.0 0.0 4.043383071887089 1.7713160169479517E-4 88-89 8.856580084739759E-4 0.0 0.0 4.785210219784892 1.7713160169479517E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTGC 75 6.909615E-6 38.00514 9 CTGGTCG 120 3.781679E-9 35.629818 9 ATACGTC 110 6.9936505E-8 34.550125 5 GGTATCA 3470 0.0 32.740864 1 GTATCAA 5165 0.0 30.2794 1 TGGGACG 65 0.005842784 29.229544 6 TTAGACT 245 0.0 27.146526 4 CGTCTCA 150 1.057264E-6 25.336758 8 TATCAAC 6245 0.0 25.103472 2 ATCAACG 6270 0.0 24.927614 3 TCAACGC 6260 0.0 24.891544 4 CAACGCA 6365 0.0 24.48092 5 AACGCAG 6485 0.0 24.096907 6 GAGTACT 3705 0.0 23.272377 12-13 GTGTAGC 295 5.456968E-12 22.559408 1 TACCTGG 555 0.0 22.25526 2 GTACTTT 3835 0.0 22.23179 14-15 GTACATG 3705 0.0 21.554739 1 ACGCAGA 7275 0.0 21.418703 7 CGCAGAG 7300 0.0 21.345352 8 >>END_MODULE