FastQCFastQC Report
Thu 26 May 2016
SRR937285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672183
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36630.5449408866335507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30010.44645580147073044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18810.27983450935236387No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10020.14906654884161008No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9630.14326455741963126No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8480.12615612117533467No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.10532845966053886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG2100.033.9562721
TATCACG600.003936916631.6877882
GTATCAA47600.030.9601291
GGTATCA31950.030.2046051
TCGTGTC804.491789E-429.6873599
GTACATG41100.028.916531
TACATGG41850.027.9397682
ACATGGG40350.026.7402523
TCCAACG908.9088874E-426.406492
AACGCGA559.4780866E-525.9089776-77
CATGGGG12650.025.548724
TATCAAC57150.025.5332032
ATCAACG57500.025.295123
TCAACGC58000.024.9932424
CAACGCA58500.024.7777775
AACGCAG59750.024.167256
ATAACGC601.7119439E-423.74811674-75
GAGTACT29700.023.66815612-13
TACCTGG5900.023.363032
TAGGGGT2354.609319E-922.2472114
Produced by FastQC (version 0.11.5)