##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937285_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 672183 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.775532853404503 33.0 31.0 34.0 30.0 34.0 2 31.88465641053106 33.0 31.0 34.0 30.0 34.0 3 31.925444410227573 33.0 31.0 34.0 30.0 34.0 4 35.55593640422325 37.0 35.0 37.0 33.0 37.0 5 35.535343797745554 37.0 35.0 37.0 33.0 37.0 6 35.61717121676686 37.0 35.0 37.0 33.0 37.0 7 35.563916373963636 37.0 35.0 37.0 33.0 37.0 8 35.558868641426514 37.0 35.0 37.0 33.0 37.0 9 37.18328639671042 39.0 37.0 39.0 34.0 39.0 10-11 37.101789244893126 39.0 37.0 39.0 34.0 39.0 12-13 37.005107983986505 39.0 37.0 39.0 33.0 39.0 14-15 38.157759122143815 40.0 38.0 41.0 33.0 41.0 16-17 38.10357372917792 40.0 38.0 41.0 33.0 41.0 18-19 38.09224050593365 40.0 38.0 41.0 33.5 41.0 20-21 38.03814363052919 40.0 38.0 41.0 33.0 41.0 22-23 37.904743946217025 40.0 38.0 41.0 33.0 41.0 24-25 37.75885956651686 40.0 37.5 41.0 33.0 41.0 26-27 37.599576305857184 40.0 37.0 41.0 32.5 41.0 28-29 37.422199758101584 39.5 37.0 41.0 32.0 41.0 30-31 37.411143245217445 39.5 37.0 41.0 32.0 41.0 32-33 37.25742692094266 39.0 36.5 41.0 32.0 41.0 34-35 37.05596095111004 39.0 36.0 41.0 31.0 41.0 36-37 37.063213440387514 39.0 36.0 41.0 31.0 41.0 38-39 37.039698266692255 40.0 36.0 41.0 31.0 41.0 40-41 36.8797552154696 39.5 36.0 41.0 31.0 41.0 42-43 36.642312435750384 39.0 36.0 41.0 30.5 41.0 44-45 36.3860057454592 39.0 36.0 41.0 30.0 41.0 46-47 36.02241205148003 39.0 35.5 41.0 29.5 41.0 48-49 35.685997711932615 39.0 35.0 41.0 27.5 41.0 50-51 34.77054462847171 38.0 34.0 40.0 26.0 40.5 52-53 34.92065479192422 38.5 34.5 40.0 26.5 40.5 54-55 35.288209460816475 39.0 35.0 40.0 27.0 41.0 56-57 35.08604353278795 39.0 35.0 40.0 26.0 41.0 58-59 34.86122901055218 38.5 35.0 40.0 26.0 41.0 60-61 34.59024774503372 38.0 34.0 40.0 25.0 41.0 62-63 34.296666235236536 38.0 34.0 40.0 23.5 41.0 64-65 33.958062759694904 37.5 34.0 40.0 23.0 41.0 66-67 33.56712978459735 37.0 33.5 40.0 22.0 41.0 68-69 33.09425781372037 36.0 33.0 39.0 20.5 41.0 70-71 32.66749977312726 36.0 32.0 39.0 19.0 40.0 72-73 32.25094579898629 35.5 32.0 38.5 18.0 40.0 74-75 31.791813390103584 35.0 31.5 37.5 14.5 39.5 76-77 31.352056657190083 35.0 31.0 37.0 10.0 39.0 78-79 30.879635456415887 35.0 31.0 36.5 7.5 39.0 80-81 30.40778329710808 34.5 30.5 36.0 4.5 38.5 82-83 29.951211946746646 34.0 30.0 36.0 2.0 37.0 84-85 29.477265119766493 34.0 29.5 35.0 2.0 37.0 86-87 29.045695889363465 34.0 29.0 35.0 2.0 36.0 88-89 28.658411474256265 34.0 29.0 35.0 2.0 36.0 90-91 28.22520652857927 34.0 28.5 35.0 2.0 36.0 92-93 27.790907386827694 34.0 27.5 35.0 2.0 35.0 94-95 27.258665422957733 33.5 26.5 35.0 2.0 35.0 96-97 26.673361272153564 33.0 25.0 35.0 2.0 35.0 98-99 25.965198465298883 33.0 24.5 35.0 2.0 35.0 100-101 24.12659796513747 30.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04103119550779866 1101 2 -0.06818022291607306 1101 3 0.09505205481743673 1101 4 0.06513923681256983 1101 5 -0.005399659678694491 1101 6 -0.043007498973445024 1101 7 0.11474673958268511 1101 8 0.009527560252891476 1101 9 0.017267871409025304 1101 10-11 -0.02496912742437729 1101 12-13 -0.024988585749177616 1101 14-15 0.20492322037311084 1101 16-17 0.006353581172156453 1101 18-19 0.08557774859946932 1101 20-21 0.054722008827539526 1101 22-23 -0.10433355974359415 1101 24-25 0.1942010230346014 1101 26-27 -0.05151348254515398 1101 28-29 -0.2212494306092765 1101 30-31 -0.3356859187301069 1101 32-33 -0.11024848969525891 1101 34-35 -0.2254674374823935 1101 36-37 -0.30316988462310235 1101 38-39 -0.08219914315620969 1101 40-41 0.0031548592016790167 1101 42-43 0.023340550259412396 1101 44-45 0.08686118687868571 1101 46-47 0.27327420825407245 1101 48-49 0.34275441659490014 1101 50-51 0.24832082164659397 1101 52-53 0.2723575867088712 1101 54-55 0.2286499546692582 1101 56-57 0.3235753641765058 1101 58-59 0.5922112234939334 1101 60-61 0.5129993620476796 1101 62-63 0.4563662917927829 1101 64-65 0.20153485465434784 1101 66-67 0.4168117869163126 1101 68-69 0.2080275143081849 1101 70-71 0.26462750538432545 1101 72-73 0.410320175368291 1101 74-75 0.7521690192527721 1101 76-77 0.6145026912110119 1101 78-79 0.7784557657105999 1101 80-81 0.8401713761859604 1101 82-83 1.06136114366981 1101 84-85 0.9251248159120031 1101 86-87 1.1250654900053512 1101 88-89 1.108052406762532 1101 90-91 0.9466746425059434 1101 92-93 1.2471377799744445 1101 94-95 1.2681728459182011 1101 96-97 1.2081919795848854 1101 98-99 1.1109166564362312 1101 100-101 1.2196663068371159 1102 1 0.07139169087525943 1102 2 -0.015297198531129652 1102 3 -0.022006292222240376 1102 4 -0.14998984977694363 1102 5 -0.019175152242695503 1102 6 0.1379125300691868 1102 7 0.13374792993539586 1102 8 0.07786541297515015 1102 9 0.29293824743206187 1102 10-11 0.13546856180959566 1102 12-13 0.23258262549644115 1102 14-15 0.23017746686996787 1102 16-17 0.1519723670123838 1102 18-19 0.13929241463390696 1102 20-21 0.2767153803904563 1102 22-23 0.29644980100243146 1102 24-25 0.38846498004360797 1102 26-27 0.2777338786750292 1102 28-29 0.23081108968845854 1102 30-31 0.31117014243722707 1102 32-33 0.4307557037049534 1102 34-35 0.33093787020649756 1102 36-37 0.29219848261799797 1102 38-39 0.6980171701090612 1102 40-41 0.5574116049453082 1102 42-43 0.3260945374470694 1102 44-45 0.644713069463478 1102 46-47 0.6627566270902889 1102 48-49 0.5363429348774318 1102 50-51 0.5727005787356134 1102 52-53 0.4837884301799633 1102 54-55 0.501776630918016 1102 56-57 0.4804327342379011 1102 58-59 0.534043848048789 1102 60-61 0.5070994603382104 1102 62-63 0.5663152449245032 1102 64-65 0.47730957176035815 1102 66-67 0.570349381811134 1102 68-69 0.5646733621504865 1102 70-71 0.44337180675595533 1102 72-73 0.4380779904536638 1102 74-75 0.35565499926868327 1102 76-77 0.4923526228027768 1102 78-79 0.7681290855104663 1102 80-81 0.45915293312779326 1102 82-83 0.6471863149373611 1102 84-85 0.7097067847850163 1102 86-87 0.5802593298065943 1102 88-89 0.6085450942430803 1102 90-91 0.7757991405574209 1102 92-93 0.04952194285025868 1102 94-95 0.3140082166809641 1102 96-97 0.622653900646224 1102 98-99 0.6985619313286762 1102 100-101 0.8605582563977734 1103 1 0.11928710161333456 1103 2 0.0995280726892318 1103 3 -0.03652184449668283 1103 4 0.018848790689531825 1103 5 -0.06392002342384018 1103 6 -0.10381099530527393 1103 7 -0.08452029705340891 1103 8 0.005763476799167222 1103 9 0.06162664248809335 1103 10-11 0.019891342589708927 1103 12-13 0.1684478775684468 1103 14-15 0.12075785825781082 1103 16-17 0.2613206018450924 1103 18-19 0.1987222606972452 1103 20-21 0.1557231262478922 1103 22-23 0.2122143223222821 1103 24-25 0.31376835465357544 1103 26-27 0.22163408803812246 1103 28-29 0.10925145004674164 1103 30-31 0.11385913155380933 1103 32-33 0.2398676120235237 1103 34-35 0.10012443982405017 1103 36-37 0.2894841161576167 1103 38-39 0.3197556046729986 1103 40-41 0.3681247371439653 1103 42-43 0.28878623732537534 1103 44-45 0.10834704741979095 1103 46-47 -0.0895547298478192 1103 48-49 -0.12086033764639836 1103 50-51 -0.1077828019879874 1103 52-53 0.029678165621156438 1103 54-55 0.07529306563191795 1103 56-57 0.14179803533431823 1103 58-59 0.3776437107804256 1103 60-61 0.17440825070404742 1103 62-63 0.19621191876218802 1103 64-65 0.13110853128926436 1103 66-67 0.249607873128042 1103 68-69 0.09687302993798852 1103 70-71 0.12050710837144862 1103 72-73 0.29098960808629926 1103 74-75 0.3818823398960767 1103 76-77 0.48068120749677945 1103 78-79 0.7490513789302362 1103 80-81 0.6989753205590823 1103 82-83 0.6410279833823616 1103 84-85 0.5525301873100652 1103 86-87 0.8128772911984257 1103 88-89 0.5685858710205025 1103 90-91 0.8854948719728419 1103 92-93 0.880745303848741 1103 94-95 0.8905893982270214 1103 96-97 0.8910820337388579 1103 98-99 1.2046337492116308 1103 100-101 1.5593875822255647 1104 1 0.022696292679633245 1104 2 -0.030059968700534512 1104 3 -0.03906022874459936 1104 4 -0.009297307426066936 1104 5 0.17378908479241773 1104 6 0.025304624571688805 1104 7 -0.07106157223893916 1104 8 0.07073553694477397 1104 9 0.17078597898265713 1104 10-11 -0.0016655330823382997 1104 12-13 0.0892370682821948 1104 14-15 0.14102967690436685 1104 16-17 0.13193439725838374 1104 18-19 0.1925490512276653 1104 20-21 0.21360445678430295 1104 22-23 0.09252950503307034 1104 24-25 0.04759232475705488 1104 26-27 0.19575490807725515 1104 28-29 0.20088800277991226 1104 30-31 0.2370236505828771 1104 32-33 0.08368294456949599 1104 34-35 0.24069804415340457 1104 36-37 0.07096625051570271 1104 38-39 0.14192416043812983 1104 40-41 0.12108882958346356 1104 42-43 0.05072457574134859 1104 44-45 0.18869307855025852 1104 46-47 0.3506257774764805 1104 48-49 0.4372889334495156 1104 50-51 0.37386238830269747 1104 52-53 0.7546847759968003 1104 54-55 0.770144905079114 1104 56-57 0.7554700549362892 1104 58-59 0.3855999589074841 1104 60-61 0.8096080603252318 1104 62-63 0.9411043018466856 1104 64-65 0.8423920018246136 1104 66-67 0.729982989911349 1104 68-69 0.705997111095769 1104 70-71 0.6424923363853381 1104 72-73 0.6927885116453254 1104 74-75 0.7611865758247909 1104 76-77 0.8501721204337223 1104 78-79 0.9927380294969659 1104 80-81 0.8116566651976278 1104 82-83 0.8812455088102773 1104 84-85 1.0284904545455156 1104 86-87 0.9056860838794201 1104 88-89 0.7574319638478073 1104 90-91 0.9929293411156088 1104 92-93 1.1705221375669446 1104 94-95 1.0217498276370236 1104 96-97 1.013997121260232 1104 98-99 1.1226386497034397 1104 100-101 1.2137856854884461 1105 1 0.1677500471591742 1105 2 0.3088627193012421 1105 3 0.1229254922877061 1105 4 0.216835425857262 1105 5 0.35291512307338735 1105 6 0.048312097416648214 1105 7 0.10518922643872486 1105 8 0.34310823466957885 1105 9 0.4089863845720103 1105 10-11 0.2175805151994581 1105 12-13 0.3372202335459633 1105 14-15 0.3848416612363863 1105 16-17 0.5195736449734696 1105 18-19 0.5720278966870112 1105 20-21 0.6622487882563703 1105 22-23 0.5194067448030495 1105 24-25 0.42961582671554055 1105 26-27 0.5377639643875014 1105 28-29 0.33391714409724216 1105 30-31 0.46613996583136696 1105 32-33 0.2873554728357348 1105 34-35 0.6893520986579489 1105 36-37 0.6522896941360088 1105 38-39 0.721002972561358 1105 40-41 0.618263594147173 1105 42-43 0.6433295150418488 1105 44-45 0.7553416881566122 1105 46-47 0.2635074065011196 1105 48-49 -0.29979332340523257 1105 50-51 -0.4045010864313099 1105 52-53 -0.0942526354541613 1105 54-55 -0.10010122654296794 1105 56-57 0.05042519008643609 1105 58-59 0.01808183239694472 1105 60-61 0.13162795087166046 1105 62-63 0.4770795220039119 1105 64-65 0.34176139371837877 1105 66-67 0.4047586656621007 1105 68-69 0.3576625276582561 1105 70-71 0.22780222332036004 1105 72-73 0.20374914725385196 1105 74-75 0.2232903622514435 1105 76-77 0.5494234469331545 1105 78-79 0.5498872363753158 1105 80-81 0.4020003049453855 1105 82-83 0.5819316771020802 1105 84-85 0.4911331747722052 1105 86-87 0.40564858075416055 1105 88-89 0.4987117927224318 1105 90-91 0.6772377557057148 1105 92-93 0.2769962881906096 1105 94-95 0.5844967232116538 1105 96-97 0.680829079478972 1105 98-99 0.6888386108113131 1105 100-101 1.0774673811853077 1106 1 0.03747320354530714 1106 2 0.20286467749800252 1106 3 0.023256115363214036 1106 4 0.09311428442720882 1106 5 0.26603078439048744 1106 6 -0.004715303148110195 1106 7 0.1654534411024997 1106 8 0.08909605979409463 1106 9 0.2411916262957945 1106 10-11 0.07396522991289345 1106 12-13 -0.05097808354000932 1106 14-15 0.09450351755015873 1106 16-17 -0.003832482195804232 1106 18-19 0.07800181577658805 1106 20-21 -0.13064352511187138 1106 22-23 0.11839500981233897 1106 24-25 -0.025998380143811062 1106 26-27 0.1310337272754154 1106 28-29 0.19677242200509681 1106 30-31 0.38859253631935786 1106 32-33 0.3326139406177262 1106 34-35 0.3955161614905194 1106 36-37 0.005623595062608899 1106 38-39 0.18666358016994877 1106 40-41 0.1646359637071484 1106 42-43 -0.019073107239023557 1106 44-45 -0.18535191081036828 1106 46-47 0.008544512910667379 1106 48-49 -0.331642400356678 1106 50-51 -0.18898592459628105 1106 52-53 0.023246264833893804 1106 54-55 -0.12201742139773586 1106 56-57 -0.37963313012129873 1106 58-59 -0.2805786475031553 1106 60-61 -0.20325207410243706 1106 62-63 -0.2277577915771758 1106 64-65 -0.3704191576984712 1106 66-67 -0.16639624472910697 1106 68-69 5.896061138415121E-4 1106 70-71 0.008399047636054036 1106 72-73 -0.06621949321838372 1106 74-75 -0.17800516937059996 1106 76-77 -0.2903875445103772 1106 78-79 -0.050420682969683384 1106 80-81 -0.3766737971930212 1106 82-83 -0.2592415381718567 1106 84-85 -0.13153082420612705 1106 86-87 -0.22860141569838532 1106 88-89 0.06683007915837536 1106 90-91 -0.039696389285985845 1106 92-93 0.02637548305956372 1106 94-95 -0.09504012068600787 1106 96-97 0.12761721110384983 1106 98-99 0.07705821264463708 1106 100-101 0.5542102472474895 1107 1 0.21725980904351871 1107 2 0.10916572361762533 1107 3 0.2161279048881113 1107 4 -0.0060585512173148 1107 5 0.09074492612057838 1107 6 0.12299122727971934 1107 7 -0.0030204754164628866 1107 8 0.1277607801916929 1107 9 0.21542093849820532 1107 10-11 0.21898834185812177 1107 12-13 0.5283845133390059 1107 14-15 0.5201716786734281 1107 16-17 0.49323942549543887 1107 18-19 0.41971105707919776 1107 20-21 0.45554839092051935 1107 22-23 0.5786355130619114 1107 24-25 0.5420775938595881 1107 26-27 0.6278362852290584 1107 28-29 0.497132134235315 1107 30-31 0.34476672740127157 1107 32-33 0.27161925810286647 1107 34-35 0.49885957371726164 1107 36-37 0.3888474507606574 1107 38-39 0.1772102732502674 1107 40-41 0.14784939296374233 1107 42-43 -0.006378914766912658 1107 44-45 -0.09722241440749002 1107 46-47 -0.38415002710949153 1107 48-49 -0.3341099915011583 1107 50-51 -0.5571609497935199 1107 52-53 -0.7468767671752801 1107 54-55 -0.41232430632457806 1107 56-57 -0.3615606315983868 1107 58-59 -0.43628886194030514 1107 60-61 -0.7056501556372083 1107 62-63 -0.6624882680655446 1107 64-65 -0.3434928726395654 1107 66-67 -0.1696631963851729 1107 68-69 -0.17654694551442418 1107 70-71 -0.6452432559124084 1107 72-73 -0.5482947026160829 1107 74-75 -0.3610934813297284 1107 76-77 -0.3640416646924898 1107 78-79 -0.16192278563537243 1107 80-81 -0.17540872229641735 1107 82-83 -0.7344005414040531 1107 84-85 -0.44124826764268477 1107 86-87 -0.24382402022345673 1107 88-89 -0.3538594561698858 1107 90-91 -0.38333452225786857 1107 92-93 0.08361664665043023 1107 94-95 0.22552951056551152 1107 96-97 0.19911653338515478 1107 98-99 -0.11208612405991403 1107 100-101 -0.2268518595887805 1108 1 -0.19155661607725705 1108 2 -0.2851579478799593 1108 3 -0.350297215884833 1108 4 -0.27357062851200453 1108 5 -0.20756425262337785 1108 6 -0.14270925581206484 1108 7 -0.2336968039188747 1108 8 -0.1723599927551689 1108 9 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-1.0832542418927105 2316 100-101 -1.6099691724261618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2549.0 3 486.0 4 420.0 5 449.0 6 478.0 7 585.0 8 748.0 9 942.0 10 1210.0 11 1510.0 12 2142.0 13 2742.0 14 3287.0 15 4325.0 16 5618.0 17 7674.0 18 6417.0 19 4058.0 20 3787.0 21 4033.0 22 4211.0 23 4717.0 24 5317.0 25 6024.0 26 6870.0 27 8282.0 28 9850.0 29 11771.0 30 14408.0 31 18269.0 32 23558.0 33 31204.0 34 43369.0 35 62550.0 36 93363.0 37 136892.0 38 120818.0 39 17247.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.176273447443855 19.847829428099477 15.28345835155013 21.692438772906534 2 14.607423716273463 20.846907480996194 41.36863208391508 23.177036718815263 3 18.84954286916168 26.325038001005403 30.4269090377504 24.398510092082514 4 11.80602585407039 17.06365662357655 38.98546790019959 32.14484962215347 5 12.511298516501054 38.687612052944175 34.5003743798867 14.300715050668067 6 29.537594657444398 37.309492576471285 18.489952894878066 14.66295987120625 7 26.90302056344495 34.05013700018784 21.48219593859291 17.564646497774305 8 25.860686163726402 34.62526945357945 20.521405720316874 18.992638662377274 9 26.31968098982596 16.78619610181493 21.312115678455633 35.58200722990348 10-11 23.983938006130213 27.643505432513983 27.790797524061684 20.58175903729412 12-13 25.103258272774774 25.337988683146744 28.603715583371038 20.95503746070744 14-15 22.240283212222845 26.188038010061486 27.576299608719957 23.995379168995715 16-17 20.57045361542458 29.749724023348257 28.104448497650935 21.575373863576225 18-19 20.39510703455081 28.038111052398975 31.175997549492717 20.390784363557497 20-21 21.647488304043446 26.899321235772746 31.017222142307205 20.43596831787661 22-23 21.162153540689683 26.86663943785421 31.04300761114871 20.928199410307396 24-25 20.813006312369115 27.079690865323702 31.231843554601618 20.875459267705562 26-27 20.634582274329752 27.182541161388418 31.279748373295966 20.90312819098586 28-29 20.343542259678056 27.44462307767415 31.346405111842042 20.86542955080575 30-31 20.599802023888323 27.12257822143509 31.31361920606809 20.964000548608503 32-33 20.391960720443244 27.30173685469859 31.183328699434913 21.122973725423254 34-35 20.859155884858563 27.314452156945464 30.959037452047383 20.867354506148594 36-37 20.82717520680498 27.01571039625914 31.144431451201175 21.0126829457347 38-39 20.576488973780172 27.422276906383512 31.054956340029825 20.94627777980649 40-41 20.688632371430128 27.47322850548397 30.97988625887694 20.85825286420896 42-43 20.55965485863677 27.466357436539884 30.64336907813004 21.330618626693308 44-45 20.64607254527092 27.56653213033254 30.455448348855324 21.331946975541218 46-47 20.811567588274706 27.85647688203979 30.02752377680177 21.304431752883737 48-49 20.91374659657455 28.00352994753526 29.758516110230364 21.324207345659826 50-51 20.834215367368415 28.19850387150826 29.677637679301373 21.28964308182195 52-53 20.99685960972097 28.43361387884042 29.338907967109762 21.23061854432885 54-55 20.70780746957003 28.169848746536587 29.49297179399831 21.629371989895066 56-57 20.945956519892302 28.37318103198373 29.229503517770684 21.45135893035328 58-59 21.13502964585185 28.02462576629516 29.408968196189445 21.43137639166354 60-61 20.951193593628208 28.16596294546446 29.836263724799057 21.046579736108274 62-63 20.801214982800662 28.170774238549175 29.84554873941063 21.18246203923953 64-65 20.66488666061077 28.142700546893572 29.758491977677142 21.433920814818514 66-67 20.642356699053295 28.238147158053046 29.35097320110388 21.76852294178978 68-69 20.734117373529926 28.10278865342512 29.547788347896848 21.615305625148107 70-71 20.71708990750208 28.31433494203603 29.38046067124337 21.588114479218522 72-73 20.806620149330488 28.157323201080402 29.28989760919177 21.746159040397337 74-75 20.55101497856319 28.589016217150785 29.239461013182538 21.62050779110348 76-77 20.60506596667904 28.18665044889539 29.396201046702114 21.81208253772346 78-79 20.78712940280387 28.055198030685947 29.14985761525488 22.007814951255302 80-81 20.676210710184446 28.34902851811473 29.04072451466892 21.93403625703191 82-83 20.750027203522595 28.21124541452951 29.377344906382618 21.661382475565276 84-85 20.617021752252356 28.193273866744168 29.180657709418522 22.009046671584954 86-87 20.50548882226738 28.33016247748543 29.114790943350112 22.04955775689708 88-89 20.716756768036294 27.938877138696487 29.098196333045667 22.246169760221548 90-91 20.478392807638308 27.81793738281262 29.420255941717073 22.283413867832007 92-93 20.622120931854816 27.751217210949196 29.49425285643059 22.132409000765392 94-95 20.330272275641263 27.647223658930304 29.539976837119035 22.482527228309394 96-97 20.22453656264555 27.678360503027484 29.716404285048903 22.380698649278063 98-99 20.125591645308685 27.9031516408073 29.246934548806873 22.724322165077147 100-101 20.120136455698844 27.764099751560018 29.628964495586352 22.48679929715479 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 945.0 1 554.5 2 178.5 3 199.5 4 179.5 5 184.0 6 307.0 7 576.5 8 900.5 9 1143.5 10 1127.0 11 1026.5 12 1084.0 13 1164.5 14 1248.5 15 1323.0 16 1360.0 17 1431.0 18 1521.5 19 1602.0 20 1732.5 21 1992.5 22 2333.0 23 2715.5 24 3175.5 25 3867.0 26 4819.0 27 5974.0 28 6893.5 29 7953.0 30 9628.0 31 11344.0 32 13059.0 33 14765.0 34 16952.5 35 18678.5 36 20462.0 37 22648.0 38 23689.0 39 23675.0 40 23351.0 41 23545.5 42 23732.0 43 24360.0 44 25405.0 45 26010.5 46 25512.5 47 25531.0 48 27471.5 49 26572.0 50 23342.5 51 20965.0 52 19012.0 53 17904.5 54 16788.0 55 15221.5 56 13371.5 57 11558.5 58 10057.5 59 8689.5 60 7266.0 61 5776.5 62 4480.0 63 3363.5 64 2436.0 65 1779.5 66 1276.5 67 959.0 68 708.5 69 480.0 70 336.5 71 231.0 72 185.0 73 168.5 74 126.0 75 90.0 76 64.0 77 39.5 78 24.0 79 12.5 80 11.0 81 6.5 82 4.5 83 3.5 84 2.0 85 2.0 86 2.5 87 2.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29962078779141993 2 0.26644529837856656 3 0.2686768335408661 4 0.26852806453004613 5 0.259006847837568 6 0.1997967815312199 7 0.20545000394237878 8 0.20545000394237878 9 0.20202831669351948 10-11 0.20931799822369804 12-13 0.21028499679402782 14-15 0.19458986615252097 16-17 0.20582192646942868 18-19 0.19362286758219116 20-21 0.19808593790679027 22-23 0.19711893933646046 24-25 0.19012679582792186 26-27 0.2005406265853198 28-29 0.20061501109072977 30-31 0.20753277009385837 32-33 0.20849976866418818 34-35 0.19912732098253003 36-37 0.19659824779859053 38-39 0.1873745691277524 40-41 0.189457335279232 42-43 0.18886225923595212 44-45 0.2024002392205694 46-47 0.19466425065793094 48-49 0.20106131812318967 50-51 0.20604507998565866 52-53 0.209243613718288 54-55 0.21147514888058758 56-57 0.18335780583561323 58-59 0.1753242792513348 60-61 0.18239080726528342 62-63 0.1825395762761034 64-65 0.18023365660839386 66-67 0.19020118033333183 68-69 0.18060557913544378 70-71 0.18581249451414272 72-73 0.19667263230400056 74-75 0.18707703110611246 76-77 0.1954824802174408 78-79 0.19183763945235152 80-81 0.18201888473823347 82-83 0.1954824802174408 84-85 0.18120065517872364 86-87 0.18276272979233335 88-89 0.18923418176300205 90-91 0.18581249451414272 92-93 0.19109379439825167 94-95 0.19072187187120174 96-97 0.18529180297627285 98-99 0.19020118033333183 100-101 0.1779277369406843 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 672183.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.18612279881599 #Duplication Level Percentage of deduplicated Percentage of total 1 85.28856626052325 58.15496652374993 2 9.090438121801888 12.396834601364434 3 2.3194784363689327 4.744687244743732 4 1.0226318262492247 2.7891719713042833 5 0.5465290869639986 1.8632849718425997 6 0.3363321913991226 1.3759912862361274 7 0.2246122075333689 1.0720804895488403 8 0.1717592396383373 0.9369277284648743 9 0.13124009627309802 0.8053877988545304 >10 0.8033041918509574 9.932717327870803 >50 0.047034916751684135 2.1642137534768757 >100 0.016331407813097748 1.8905540393311924 >500 8.710084165374048E-4 0.45567504484758486 >1k 8.710084165374048E-4 1.417507218364253 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3663 0.5449408866335507 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3001 0.44645580147073044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1881 0.27983450935236387 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1002 0.14906654884161008 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 963 0.14326455741963126 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 848 0.12615612117533467 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 708 0.10532845966053886 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.4876901081997015E-4 0.0 0.0 0.0 0.0 7 1.4876901081997015E-4 0.0 0.0 0.0 0.0 8 1.4876901081997015E-4 0.0 0.0 1.4876901081997015E-4 0.0 9 1.4876901081997015E-4 0.0 0.0 1.4876901081997015E-4 0.0 10-11 1.4876901081997015E-4 0.0 0.0 1.4876901081997015E-4 0.0 12-13 1.4876901081997015E-4 0.0 0.0 1.4876901081997015E-4 0.0 14-15 1.4876901081997015E-4 0.0 0.0 1.4876901081997015E-4 0.0 16-17 1.4876901081997015E-4 0.0 0.0 1.4876901081997015E-4 0.0 18-19 1.4876901081997015E-4 0.0 0.0 1.4876901081997015E-4 0.0 20-21 1.4876901081997015E-4 0.0 0.0 3.7192252704992536E-4 0.0 22-23 1.4876901081997015E-4 0.0 0.0 5.950760432798806E-4 0.0 24-25 1.4876901081997015E-4 0.0 0.0 0.0011901520865597612 0.0 26-27 1.4876901081997015E-4 0.0 0.0 0.001933997140659612 0.0 28-29 1.4876901081997015E-4 0.0 0.0 0.004016763292139194 0.0 30-31 1.4876901081997015E-4 0.0 0.0 0.007735988562638448 0.0 32-33 1.4876901081997015E-4 0.0 0.0 0.0134635954792073 0.0 34-35 1.4876901081997015E-4 0.0 0.0 0.02172027557971564 0.0 36-37 1.4876901081997015E-4 0.0 0.0 0.02767103601251445 0.0 38-39 1.4876901081997015E-4 0.0 0.0 0.03347302743449329 0.0 40-41 1.4876901081997015E-4 0.0 0.0 0.04225039907287152 0.0 42-43 1.4876901081997015E-4 0.0 0.0 0.052664229830269436 0.0 44-45 1.4876901081997015E-4 0.0 0.0 0.06553274926619686 0.0 46-47 1.4876901081997015E-4 0.0 0.0 0.08182295595098359 0.0 48-49 1.4876901081997015E-4 0.0 0.0 0.09550970494642085 0.0 50-51 1.4876901081997015E-4 0.0 0.0 0.1121718341582575 0.0 52-53 1.4876901081997015E-4 0.0 0.0 0.16163753025589758 0.0 54-55 1.4876901081997015E-4 0.0 0.0 0.23698903423621248 0.0 56-57 1.4876901081997015E-4 0.0 0.0 0.2947114104343609 0.0 58-59 1.4876901081997015E-4 0.0 0.0 0.38917973230504194 0.0 60-61 1.4876901081997015E-4 0.0 0.0 0.5226255350105552 0.0 62-63 1.4876901081997015E-4 0.0 0.0 0.6065312571130184 0.0 64-65 1.4876901081997015E-4 0.0 0.0 0.6717664683575753 0.0 66-67 1.4876901081997015E-4 0.0 0.0 0.867695255607476 0.0 68-69 1.4876901081997015E-4 0.0 0.0 1.0459949150752101 0.0 70-71 1.4876901081997015E-4 0.0 0.0 1.1370415496970319 0.0 72-73 1.4876901081997015E-4 0.0 0.0 1.3337885665064424 0.0 74-75 1.4876901081997015E-4 0.0 0.0 1.6180117616779954 0.0 76-77 1.4876901081997015E-4 0.0 0.0 1.9226163113318844 0.0 78-79 1.4876901081997015E-4 0.0 0.0 2.198508441897519 0.0 80-81 2.975380216399403E-4 0.0 0.0 2.4985011522159892 1.4876901081997015E-4 82-83 2.975380216399403E-4 0.0 0.0 2.787038648701321 2.975380216399403E-4 84-85 2.975380216399403E-4 0.0 0.0 3.121099462497564 2.975380216399403E-4 86-87 2.975380216399403E-4 0.0 0.0 3.708588286225626 2.975380216399403E-4 88-89 2.975380216399403E-4 0.0 0.0 4.375222223709913 2.975380216399403E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 210 0.0 33.956272 1 TATCACG 60 0.0039369166 31.687788 2 GTATCAA 4760 0.0 30.960129 1 GGTATCA 3195 0.0 30.204605 1 TCGTGTC 80 4.491789E-4 29.687359 9 GTACATG 4110 0.0 28.91653 1 TACATGG 4185 0.0 27.939768 2 ACATGGG 4035 0.0 26.740252 3 TCCAACG 90 8.9088874E-4 26.40649 2 AACGCGA 55 9.4780866E-5 25.90897 76-77 CATGGGG 1265 0.0 25.54872 4 TATCAAC 5715 0.0 25.533203 2 ATCAACG 5750 0.0 25.29512 3 TCAACGC 5800 0.0 24.993242 4 CAACGCA 5850 0.0 24.777777 5 AACGCAG 5975 0.0 24.16725 6 ATAACGC 60 1.7119439E-4 23.748116 74-75 GAGTACT 2970 0.0 23.668156 12-13 TACCTGG 590 0.0 23.36303 2 TAGGGGT 235 4.609319E-9 22.247211 4 >>END_MODULE