FastQCFastQC Report
Thu 26 May 2016
SRR937285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672183
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54080.8045428105143986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44380.6602368700190275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28530.4244379878693748No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16190.2408570285175317No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12050.17926665803806402No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.10860137789857821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG407.500712E-659.4301031
GGTATCA45500.028.1084791
GTATCAA64450.028.1059651
TAAGGGT1554.5634806E-827.591864
CCGTCTA700.00838772427.1437939
CTACACG1501.0552685E-625.3436344
GTACATG39850.025.1739041
TACCTGG6300.024.891072
TACATGG40950.024.2528362
ACATGGG40900.023.1206443
ATCAACG78350.023.1076683
GAGTACT42850.022.94711312-13
GGGGCGC1452.3331988E-522.9301196
TCAACGC79200.022.8596694
CAACGCA79400.022.8020885
AGTACTT44250.022.65049712-13
TATCAAC79800.022.5686952
AACGCAG80700.022.542476
GTACTTT44800.021.84064714-15
GTACCTG7650.021.1307031
Produced by FastQC (version 0.11.5)