##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937285_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 672183 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30516540882468 33.0 31.0 34.0 31.0 34.0 2 32.509700483350514 34.0 31.0 34.0 31.0 34.0 3 32.58420846703948 34.0 31.0 34.0 31.0 34.0 4 36.17485714455736 37.0 35.0 37.0 35.0 37.0 5 36.102705661999785 37.0 35.0 37.0 35.0 37.0 6 36.204072402902185 37.0 36.0 37.0 35.0 37.0 7 36.16251080434941 37.0 36.0 37.0 35.0 37.0 8 36.15075507711442 37.0 36.0 37.0 35.0 37.0 9 37.865268833041 39.0 38.0 39.0 35.0 39.0 10-11 37.80203530883703 39.0 37.5 39.0 35.0 39.0 12-13 37.74075809712534 39.0 37.0 39.0 35.0 39.0 14-15 38.95096127096341 40.0 38.0 41.0 36.0 41.0 16-17 38.93451336912716 40.0 38.0 41.0 36.0 41.0 18-19 38.977079902348024 40.0 38.0 41.0 36.0 41.0 20-21 38.90426342231208 40.0 38.0 41.0 35.0 41.0 22-23 38.78471933982264 40.0 38.0 41.0 35.0 41.0 24-25 38.6501347103393 40.0 38.0 41.0 34.5 41.0 26-27 38.60042652075402 40.0 38.0 41.0 34.0 41.0 28-29 38.505932164306444 40.0 38.0 41.0 34.0 41.0 30-31 38.539286176532286 40.0 38.0 41.0 35.0 41.0 32-33 38.4196796110583 40.0 38.0 41.0 34.5 41.0 34-35 38.28716659005063 40.0 38.0 41.0 34.0 41.0 36-37 38.13563940177006 40.0 38.0 41.0 34.0 41.0 38-39 38.00256031467622 40.0 37.0 41.0 34.0 41.0 40-41 37.82496879569998 40.0 37.0 41.0 33.0 41.0 42-43 37.58444277823152 40.0 37.0 41.0 33.0 41.0 44-45 37.22165243691078 40.0 37.0 41.0 32.5 41.0 46-47 36.747089706225836 40.0 37.0 41.0 31.5 41.0 48-49 36.34163687567225 40.0 36.0 41.0 30.5 41.0 50-51 36.08609857732195 40.0 36.0 41.0 30.0 41.0 52-53 35.86604987034781 39.0 36.0 41.0 29.0 41.0 54-55 35.64508474626702 39.0 35.0 41.0 28.0 41.0 56-57 35.40507198188588 39.0 35.0 40.0 28.0 41.0 58-59 35.17454249809948 39.0 35.0 40.0 27.0 41.0 60-61 34.848971187905676 38.0 34.5 40.0 26.0 41.0 62-63 34.50737745524656 38.0 34.0 40.0 26.0 41.0 64-65 34.167499029282205 37.5 34.0 40.0 25.5 41.0 66-67 33.75416292884527 37.0 34.0 39.5 24.5 41.0 68-69 33.35927344190496 36.0 33.0 39.0 23.0 40.5 70-71 32.95476678226019 36.0 33.0 39.0 22.0 40.0 72-73 32.51015794805879 35.0 32.5 38.0 21.5 40.0 74-75 32.05740177897983 35.0 32.0 37.5 20.0 39.5 76-77 30.755706407332525 34.0 30.5 36.0 18.5 38.5 78-79 31.36555000647145 35.0 31.5 36.0 18.5 39.0 80-81 31.199764796193893 35.0 32.0 36.0 18.0 38.5 82-83 30.854523247389473 35.0 31.5 36.0 15.5 37.0 84-85 30.497934342284765 34.0 31.0 35.0 10.0 37.0 86-87 30.132481779515402 34.0 31.0 35.0 7.0 36.0 88-89 29.752931865280736 34.0 30.5 35.0 2.0 36.0 90-91 29.336066220062097 34.0 30.0 35.0 2.0 35.5 92-93 28.995786861613578 34.0 29.5 35.0 2.0 35.0 94-95 28.608581294082114 34.0 29.0 35.0 2.0 35.0 96-97 28.14576759602668 34.0 29.0 35.0 2.0 35.0 98-99 27.677152650394312 34.0 29.0 35.0 2.0 35.0 100-101 25.79712816301513 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08145617165972396 1101 2 0.01513023367795796 1101 3 -0.09617202453397766 1101 4 -0.027313400853230974 1101 5 -0.08963009778749864 1101 6 -0.03403769384467381 1101 7 -0.04443237981406156 1101 8 -0.07376630193832767 1101 9 -0.05531571647728839 1101 10-11 -0.14276243096039565 1101 12-13 -0.06823226738233501 1101 14-15 -0.11849051684455247 1101 16-17 -0.18811844781555465 1101 18-19 -0.31354912257003065 1101 20-21 -0.10982885518005503 1101 22-23 -0.0971089660709552 1101 24-25 0.031579541934846134 1101 26-27 -0.25194974014174676 1101 28-29 -0.35384777407276147 1101 30-31 -0.09007355008360918 1101 32-33 -0.1576661762239766 1101 34-35 -0.3075013074426707 1101 36-37 -0.13024155752132316 1101 38-39 -0.02859778210659414 1101 40-41 -0.10562322898156395 1101 42-43 -0.0070237477545020965 1101 44-45 0.025245174867144726 1101 46-47 0.12636550937403968 1101 48-49 -0.08389822279266212 1101 50-51 -0.0825366581349698 1101 52-53 -0.2854948677606046 1101 54-55 -0.16273833818538463 1101 56-57 -0.23316490088544128 1101 58-59 -0.3979525035555014 1101 60-61 -0.164173246905996 1101 62-63 -0.11102954162428347 1101 64-65 -0.18537601549525817 1101 66-67 -0.06936325661200726 1101 68-69 -0.28442739167621767 1101 70-71 -0.37365822920912706 1101 72-73 -0.508573401155413 1101 74-75 -0.490890654584728 1101 76-77 -0.614026656130882 1101 78-79 -0.4988672987434697 1101 80-81 -0.42733382257300434 1101 82-83 -0.38999237315637103 1101 84-85 -0.4229944470785263 1101 86-87 -0.33242329693785067 1101 88-89 -0.35761120156789516 1101 90-91 -0.599439717526213 1101 92-93 -0.5525872097242406 1101 94-95 -0.6191857779448036 1101 96-97 -0.6666963673899211 1101 98-99 -0.25178545459045765 1101 100-101 0.07926085552001538 1102 1 0.15528150098241156 1102 2 0.07752867090783866 1102 3 0.06353898153633253 1102 4 0.12383422757891083 1102 5 0.035355701946777174 1102 6 0.06325511810429418 1102 7 0.00697134732351401 1102 8 0.026723422008508635 1102 9 0.009970931321547027 1102 10-11 0.17907856557720692 1102 12-13 0.1317155003358934 1102 14-15 0.11335104770368076 1102 16-17 0.20211473289579374 1102 18-19 0.1770163443594015 1102 20-21 0.09123023768228222 1102 22-23 0.11454599463753112 1102 24-25 0.1662721493999868 1102 26-27 0.06944425672678989 1102 28-29 -0.01923950383799422 1102 30-31 0.20626006478542536 1102 32-33 0.06581143180917337 1102 34-35 -0.17358662757840193 1102 36-37 -0.14047315768134894 1102 38-39 -0.09847728976485826 1102 40-41 0.12336716916918533 1102 42-43 -0.05725008764218131 1102 44-45 -0.1786113703440293 1102 46-47 0.17236202228270514 1102 48-49 0.04449811032828421 1102 50-51 0.028124160491827865 1102 52-53 0.150779896268034 1102 54-55 0.12641858586417953 1102 56-57 0.17266696302909423 1102 58-59 0.11156784851229418 1102 60-61 0.32841359697999195 1102 62-63 0.13631562608702552 1102 64-65 0.25384808198524667 1102 66-67 0.2911506201346299 1102 68-69 0.4597653170429865 1102 70-71 0.3880539030039927 1102 72-73 0.5410466491258745 1102 74-75 0.5431547912048309 1102 76-77 0.3077786545215382 1102 78-79 0.3830321781928312 1102 80-81 0.3580847271723755 1102 82-83 0.21872115685681948 1102 84-85 0.1702105405487231 1102 86-87 0.09106443650216889 1102 88-89 0.2371761895839839 1102 90-91 0.12263144679679172 1102 92-93 -5.352095859407768E-4 1102 94-95 0.14623324102674928 1102 96-97 0.42212012072383587 1102 98-99 0.522989687288721 1102 100-101 0.7784621831831231 1103 1 0.07733124057625673 1103 2 0.03185666154129052 1103 3 0.15940333963371955 1103 4 0.07901726167106915 1103 5 -0.06459210147109218 1103 6 0.027969075066629046 1103 7 0.03397082247390415 1103 8 0.0385887721293372 1103 9 -0.0170444046687237 1103 10-11 0.0660268246337452 1103 12-13 0.194294444061633 1103 14-15 0.15462888347766324 1103 16-17 0.1809448373466438 1103 18-19 0.1310768635567925 1103 20-21 0.09381194984552366 1103 22-23 0.1508550618471176 1103 24-25 0.10987677038358612 1103 26-27 0.13757521535595174 1103 28-29 -0.022857938171242154 1103 30-31 0.10026351379715237 1103 32-33 0.1475639681016574 1103 34-35 0.17653420318355018 1103 36-37 0.21284002757902698 1103 38-39 0.23970249141323308 1103 40-41 0.22252409343511914 1103 42-43 0.44536046241174176 1103 44-45 0.30039752902903416 1103 46-47 0.3974244563160241 1103 48-49 0.19348763063881336 1103 50-51 0.0667784691610791 1103 52-53 0.0741468065187405 1103 54-55 0.2208453751111108 1103 56-57 -0.08140566808237537 1103 58-59 0.22618981260335858 1103 60-61 0.04398620213735427 1103 62-63 0.005341136085121434 1103 64-65 -0.49652392680454227 1103 66-67 -0.024892475209206566 1103 68-69 -0.034527664391170276 1103 70-71 -0.17004291156260365 1103 72-73 0.04226721284896229 1103 74-75 -0.18940044777476572 1103 76-77 0.02025695400880423 1103 78-79 -0.001418719530704493 1103 80-81 0.0895931507202441 1103 82-83 -0.054444320727487394 1103 84-85 -0.020590316513388274 1103 86-87 -0.015253656967200158 1103 88-89 0.09633914096906793 1103 90-91 -0.03819891142748233 1103 92-93 -0.06188205443232064 1103 94-95 0.0932897575249072 1103 96-97 0.19963507317015683 1103 98-99 0.34371173039560077 1103 100-101 0.6566365139998673 1104 1 0.08856182810644242 1104 2 0.042976194760633746 1104 3 0.0355354766359568 1104 4 0.048108907919015564 1104 5 -0.07600601675251539 1104 6 -0.003260102250088437 1104 7 -0.020902346452047027 1104 8 0.033171789633613 1104 9 0.07523996487771711 1104 10-11 0.041491754387486424 1104 12-13 -0.030611688604196274 1104 14-15 -0.008724956709890819 1104 16-17 0.06431048807201023 1104 18-19 -0.12076875332346759 1104 20-21 -0.20032068073142995 1104 22-23 -0.05840298959041945 1104 24-25 -0.049587524774089786 1104 26-27 -0.08301566408842831 1104 28-29 -0.04696553745908005 1104 30-31 0.07283045486785511 1104 32-33 0.07950001492074676 1104 34-35 0.1637567877522912 1104 36-37 -0.1879223690787697 1104 38-39 -0.1704105019411415 1104 40-41 -0.027556112456046833 1104 42-43 0.07571707014323437 1104 44-45 0.10320209106961187 1104 46-47 0.13954576992987455 1104 48-49 0.32101852312766255 1104 50-51 -0.013480305010865834 1104 52-53 0.09890574815353403 1104 54-55 -0.06483214432339679 1104 56-57 0.23923897575058106 1104 58-59 0.08504419043480027 1104 60-61 0.11613184193358705 1104 62-63 -0.1269315992001836 1104 64-65 -0.25292805873001356 1104 66-67 -0.013675108430305727 1104 68-69 -0.0032482482195490547 1104 70-71 -0.053949731707497506 1104 72-73 0.01266506932448408 1104 74-75 -0.2244557105151621 1104 76-77 0.04886225288592172 1104 78-79 -0.0103409638854437 1104 80-81 0.1353369501878916 1104 82-83 0.036378715109560034 1104 84-85 -0.021973207098451297 1104 86-87 0.09570193893613066 1104 88-89 -0.02392915214380409 1104 90-91 -0.12523640547814452 1104 92-93 -0.24974011644646055 1104 94-95 -0.1781225712663641 1104 96-97 -0.08659507366832031 1104 98-99 0.044077504514657306 1104 100-101 0.29304646305590865 1105 1 0.08810901259403892 1105 2 -0.025623966363902184 1105 3 -0.058119550171831236 1105 4 -0.08200749066975277 1105 5 -0.11488425660584056 1105 6 -0.02321176488864296 1105 7 -0.15808877865472937 1105 8 -0.19951704554709693 1105 9 0.07127852364650522 1105 10-11 -0.0883093401481716 1105 12-13 -0.16552293120774664 1105 14-15 0.009990491799705126 1105 16-17 0.07473218988049979 1105 18-19 -0.02543164189751934 1105 20-21 0.12222147555937113 1105 22-23 0.11297240280339338 1105 24-25 0.26052859601375644 1105 26-27 -0.08146831292027201 1105 28-29 -0.14799340089214752 1105 30-31 -0.09973253649253166 1105 32-33 -0.28356912973020343 1105 34-35 -0.2920298055749271 1105 36-37 -0.1673776014592434 1105 38-39 -0.3634849248097183 1105 40-41 -0.33827922827125434 1105 42-43 -0.6021866440551733 1105 44-45 -0.5211249361826589 1105 46-47 -0.6742553801659668 1105 48-49 -0.750948585242476 1105 50-51 -0.754958761548906 1105 52-53 -0.77672694345965 1105 54-55 -0.7850421618248546 1105 56-57 -0.822600899794601 1105 58-59 -0.631594696139075 1105 60-61 -0.525928607548316 1105 62-63 -0.9473721914717643 1105 64-65 -0.9357169022447493 1105 66-67 -0.7820321937429782 1105 68-69 -0.7755167705964183 1105 70-71 -0.34564209495999165 1105 72-73 -0.46566923631209534 1105 74-75 -0.6380332308640817 1105 76-77 -0.3781108282041714 1105 78-79 -0.48054820337762294 1105 80-81 -0.5500118288770324 1105 82-83 -0.25278149268553207 1105 84-85 -0.33746199783101005 1105 86-87 -0.2836685641613492 1105 88-89 -0.3062276770208747 1105 90-91 -0.6304271158151202 1105 92-93 -0.5752950237998498 1105 94-95 -0.5209469732998677 1105 96-97 -0.39613059051694677 1105 98-99 -0.41911803955845883 1105 100-101 -0.5143735997271008 1106 1 -0.10295706945156269 1106 2 -0.1017666025508106 1106 3 0.021542164243619766 1106 4 0.025452103784857627 1106 5 -0.0108018420640974 1106 6 -0.1276631150511136 1106 7 -0.06711776848062101 1106 8 -0.08460778951996417 1106 9 -0.10781134055680752 1106 10-11 -0.15053884648392568 1106 12-13 -0.3661032953188226 1106 14-15 -0.24280135756104215 1106 16-17 -0.15012697604120007 1106 18-19 -0.0776927915576735 1106 20-21 0.001643381821345713 1106 22-23 0.044478085830938596 1106 24-25 -0.05577693943953932 1106 26-27 -0.165692058184419 1106 28-29 0.06447094715604607 1106 30-31 0.2638748024883526 1106 32-33 0.21261380557836418 1106 34-35 -0.079359704969022 1106 36-37 0.006611018295835436 1106 38-39 0.126255826967558 1106 40-41 -0.00982719699687351 1106 42-43 -0.2790132301235886 1106 44-45 0.18034938731712202 1106 46-47 0.1987243425647236 1106 48-49 0.23708229183100826 1106 50-51 -0.05200160065041359 1106 52-53 0.13299734811300823 1106 54-55 0.003685435978560747 1106 56-57 0.04109157817568132 1106 58-59 0.18465407998795058 1106 60-61 -0.0062969755757862345 1106 62-63 0.017209988937757714 1106 64-65 -0.0736917294835493 1106 66-67 -0.18632177982559028 1106 68-69 0.004356372309381129 1106 70-71 -0.051181805203633246 1106 72-73 0.0012116968112323434 1106 74-75 0.09573714928150778 1106 76-77 -0.12127487684691474 1106 78-79 -0.29041155529963447 1106 80-81 -0.15057516519023295 1106 82-83 -0.44627403592904713 1106 84-85 -0.4170030986781441 1106 86-87 -0.30375427074401173 1106 88-89 -0.16924613799174182 1106 90-91 -0.27294978407104864 1106 92-93 -0.3409854300547259 1106 94-95 -0.9859264689932523 1106 96-97 -0.7668683198600981 1106 98-99 -0.4766152370667278 1106 100-101 0.03428333271510908 1107 1 0.24874417824592854 1107 2 0.1836919870992091 1107 3 0.16601788801243345 1107 4 0.18869542217363033 1107 5 0.033302005292796366 1107 6 0.14093939424579105 1107 7 0.10736872927769525 1107 8 0.08980919862864312 1107 9 0.14164127126677784 1107 10-11 0.08238785563263917 1107 12-13 0.03146560608179527 1107 14-15 -0.0784024419109528 1107 16-17 0.19846371662205797 1107 18-19 0.32733970418486535 1107 20-21 0.2751028750962945 1107 22-23 0.1711506608144262 1107 24-25 0.008927818493241091 1107 26-27 -0.09509636082925965 1107 28-29 -0.0751651700371525 1107 30-31 -0.2269586177319951 1107 32-33 -0.4400379860318324 1107 34-35 -0.26653170649823466 1107 36-37 -0.4032350179186679 1107 38-39 -0.2765784273681007 1107 40-41 -0.41252243211312134 1107 42-43 -0.22636838947437354 1107 44-45 -0.6251540360311552 1107 46-47 -0.8625396896877291 1107 48-49 -0.9319650789679983 1107 50-51 -0.7165829790259721 1107 52-53 -0.5946813784709448 1107 54-55 -0.6892384857536129 1107 56-57 -0.7970813617332553 1107 58-59 -0.7616149917112125 1107 60-61 -0.5229271496973595 1107 62-63 -0.7033179365479398 1107 64-65 -0.7092354684054598 1107 66-67 -0.4187271598693769 1107 68-69 -0.5978661974608954 1107 70-71 -0.7966897466765417 1107 72-73 -0.7532803617158592 1107 74-75 -0.8412176347173812 1107 76-77 -0.88212706466188 1107 78-79 -0.994848006138966 1107 80-81 -0.9688509793215339 1107 82-83 -0.8946718394253814 1107 84-85 -0.8884867812972637 1107 86-87 -0.755097543951532 1107 88-89 -0.5317300638945 1107 90-91 -0.7251445499974345 1107 92-93 -0.4435381704710899 1107 94-95 -0.5945613002586754 1107 96-97 -0.7032583382801469 1107 98-99 -0.4167240196059119 1107 100-101 0.3099410673969274 1108 1 -0.06888867199561588 1108 2 0.0049480257465504 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88-89 -0.5104565356227582 2316 90-91 -0.3677889812064876 2316 92-93 -0.36809407581913334 2316 94-95 -0.5373725919383396 2316 96-97 -0.9413275782850619 2316 98-99 -0.5915706308730115 2316 100-101 -0.7788258668137225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 3.0 5 17.0 6 24.0 7 52.0 8 134.0 9 255.0 10 468.0 11 804.0 12 1394.0 13 2125.0 14 2610.0 15 4076.0 16 6043.0 17 9538.0 18 8436.0 19 4157.0 20 3138.0 21 2938.0 22 2905.0 23 3175.0 24 3397.0 25 3894.0 26 4879.0 27 5753.0 28 7248.0 29 9325.0 30 11766.0 31 15249.0 32 20125.0 33 27016.0 34 39516.0 35 59224.0 36 94795.0 37 156949.0 38 145252.0 39 15499.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74622266549563 20.435714604701012 15.586286973220002 20.231775756583353 2 15.643061487719862 21.684422248108028 40.507123804083115 22.16539246008899 3 19.616086690677985 26.808770825801904 30.655639907584693 22.91950257593542 4 12.660094051768642 18.09447724801133 38.36395743421062 30.881471266009402 5 12.5589042726522 38.895493739444845 34.207256738559515 14.338345249343442 6 29.76689383694619 38.08085000662022 17.65605497312488 14.496201183308713 7 27.23499404180112 34.76225968225914 20.828107821828283 17.174638454111456 8 26.4154255611939 35.04417695776299 19.559703235577217 18.980694245465894 9 26.846260616528532 17.174489685100635 20.79805648164265 35.18119321672818 10-11 24.47458504603657 28.06095958987359 27.000608465254256 20.463846898835587 12-13 26.072894250002417 25.57346367821615 28.03357208901677 20.32006998276466 14-15 22.816331266931773 26.48676030188208 27.541309435079437 23.155598996106715 16-17 20.677627967383884 30.040033740811655 27.9820376296336 21.30030066217087 18-19 20.443986235891117 27.762231416147092 31.907977440667203 19.88580490729459 20-21 21.750996380449966 26.471883400800078 31.986155555853095 19.790964662896858 22-23 21.295736345019908 26.69926202967502 31.886997782592946 20.118003842712124 24-25 21.11507168456115 26.64959289239059 32.25738305605187 19.97795236699639 26-27 20.854935961998216 26.829981299624055 32.32948366432156 19.985599074056168 28-29 20.422425726997297 27.167044425814773 32.41203245331693 19.998497393871002 30-31 20.760864228937656 26.754246983336383 32.338663726991015 20.14622506073495 32-33 20.68603341649521 27.064727908917664 31.939739624477266 20.309499050109867 34-35 21.07231215308926 26.944448163669716 32.02907541547466 19.954164267766366 36-37 21.18872390405589 26.644604222362066 32.02029804383628 20.146373829745766 38-39 20.83108320204468 27.128475430054017 32.058903602144056 19.981537765757242 40-41 20.94615603191393 27.237151192458008 31.827716559329826 19.98897621629824 42-43 20.80569915615173 27.271706049476307 31.485144684678385 20.437450109693582 44-45 21.028648448413602 27.155625774528662 31.27511406863905 20.54061170841869 46-47 21.354155044087697 27.641877286393736 30.591818001942922 20.412149667575648 48-49 21.455987431993968 27.943357686820402 30.142386820255794 20.458268060929836 50-51 21.24771081684601 28.21463797805062 29.99986610789026 20.53778509721311 52-53 21.463872189567425 28.515895224961056 29.41074082504318 20.609491760428337 54-55 21.202633806567555 28.369952825346665 29.511754983389938 20.915658384695835 56-57 21.52568571356312 28.39643370927262 29.41877435162746 20.659106225536796 58-59 21.67408280185604 28.095102077856776 29.500894845600083 20.729920274687103 60-61 21.322690398299272 28.271467740183848 30.09292112415815 20.312920737358723 62-63 21.37758616329184 28.102912450924823 30.00261833459043 20.516883051192906 64-65 21.116645318313616 28.269013051505322 29.792630875818045 20.821710754363025 66-67 21.13100152785774 28.26953374304319 29.521499353598646 21.077965375500423 68-69 21.205162879751498 28.329933961436097 29.535037333583265 20.92986582522914 70-71 21.326335239064363 28.41495545111971 29.40099645483447 20.857712854981454 72-73 21.39926388983936 28.155395413742113 29.336251193873085 21.10908950254544 74-75 21.221453086436284 28.764562626546642 29.041124217660965 20.972860069356113 76-77 21.245256128167476 28.35671238338369 29.31939665239972 21.078634836049112 78-79 21.36553587341542 28.16003975107969 28.99961766364219 21.474806711862694 80-81 21.275233083847702 28.5698239913833 28.930142535589265 21.224800389179734 82-83 21.41298251512613 28.551907893855972 28.98854623977768 21.046563351240216 84-85 21.026146464834305 28.497191951500728 29.075538364265256 21.401123219399707 86-87 20.863514846403437 28.61586800023208 28.90641387836348 21.614203275001003 88-89 21.162838096173214 28.496704022565282 28.81678054934445 21.523677331917053 90-91 20.869911943528404 28.170706214836365 29.129232561478474 21.830149280156757 92-93 21.017560876913187 28.051209802194666 29.10770393468377 21.823525386208374 94-95 20.78555988473377 28.085208938637248 29.26762503663437 21.861606139994613 96-97 20.93455204906997 27.990145540723283 29.286444316503097 21.78885809370365 98-99 20.776054122206393 28.31656581359973 28.727094204326946 22.180285859866927 100-101 20.297242568563824 28.944772515254176 28.60844414092315 22.14954077525885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 286.0 1 215.5 2 177.0 3 236.0 4 216.0 5 186.0 6 324.0 7 728.0 8 1254.0 9 1518.0 10 1475.0 11 1395.0 12 1414.0 13 1517.0 14 1668.5 15 1714.5 16 1721.5 17 1801.5 18 1893.5 19 1943.0 20 2076.5 21 2261.5 22 2458.5 23 2859.5 24 3316.5 25 3951.5 26 4798.5 27 5873.0 28 6839.0 29 7971.0 30 9635.5 31 11190.5 32 12791.0 33 14570.5 34 16549.5 35 18113.5 36 20196.5 37 22141.5 38 22951.5 39 23364.5 40 23245.5 41 23547.5 42 23819.5 43 24242.0 44 25155.0 45 25570.0 46 25139.0 47 25513.0 48 27435.5 49 26263.0 50 23076.0 51 20950.0 52 19071.5 53 17904.0 54 16682.5 55 15126.0 56 13240.0 57 11475.5 58 10259.0 59 8946.0 60 7405.0 61 5910.5 62 4567.0 63 3403.5 64 2445.5 65 1781.5 66 1254.5 67 914.5 68 671.0 69 432.0 70 309.5 71 244.0 72 184.0 73 161.0 74 126.0 75 98.5 76 73.0 77 37.5 78 20.0 79 11.0 80 7.0 81 4.0 82 2.5 83 3.5 84 3.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061441601468647675 2 0.0 3 0.0 4 0.0 5 0.017554743276756476 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0026034576893494777 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.2315351622995524E-4 24-25 2.9753802163994035E-4 26-27 5.950760432798807E-4 28-29 0.0026034576893494777 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.012422212403467508 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.4876901081997018E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.9753802163994035E-4 84-85 0.0011901520865597614 86-87 0.0 88-89 0.0 90-91 0.008554218122148283 92-93 0.001041383075739791 94-95 0.0 96-97 0.0 98-99 7.438450540998509E-5 100-101 0.01071136877903785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 672183.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.16870057410084 #Duplication Level Percentage of deduplicated Percentage of total 1 83.95544329637319 54.71267145747245 2 9.844298236526821 12.830802482767306 3 2.581186121539418 5.0463763644188075 4 1.100806765995786 2.8695258609249468 5 0.6021860773099735 1.9621842081052998 6 0.38890971467318225 1.5206844447537364 7 0.26122829232953376 1.1916735855014948 8 0.18789697012367446 0.9796001107816409 9 0.15339961808794014 0.8997168401418956 >10 0.841454641977405 9.990964377042074 >50 0.05561348047392608 2.4538598708354185 >100 0.025968138821642655 3.027737035910354 >500 4.596130764493957E-4 0.1894488475161303 >1k 9.192261528987914E-4 1.5148419704550655 >5k 2.2980653822469784E-4 0.8099125433733064 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5408 0.8045428105143986 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4438 0.6602368700190275 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2853 0.4244379878693748 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1619 0.2408570285175317 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1205 0.17926665803806402 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 730 0.10860137789857821 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.4876901081997015E-4 0.0 9 0.0 0.0 0.0 1.4876901081997015E-4 0.0 10-11 0.0 0.0 0.0 1.4876901081997015E-4 0.0 12-13 0.0 0.0 0.0 1.4876901081997015E-4 0.0 14-15 0.0 0.0 0.0 2.975380216399403E-4 0.0 16-17 0.0 0.0 0.0 2.975380216399403E-4 0.0 18-19 0.0 0.0 0.0 2.975380216399403E-4 0.0 20-21 0.0 0.0 0.0 4.4630703245991047E-4 0.0 22-23 0.0 0.0 0.0 5.950760432798806E-4 0.0 24-25 0.0 0.0 0.0 0.0011901520865597612 0.0 26-27 0.0 0.0 0.0 0.001933997140659612 0.0 28-29 1.4876901081997015E-4 0.0 0.0 0.003867994281319224 0.0 30-31 1.4876901081997015E-4 0.0 0.0 0.007587219551818477 0.0 32-33 1.4876901081997015E-4 0.0 0.0 0.01361236449002727 0.0 34-35 2.975380216399403E-4 0.0 0.0 0.02209219810676557 0.0 36-37 2.975380216399403E-4 0.0 0.0 0.02796857403415439 0.0 38-39 2.975380216399403E-4 0.0 0.0 0.03369618095072324 0.0 40-41 2.975380216399403E-4 0.0 0.0 0.04269670610533144 0.0 42-43 2.975380216399403E-4 0.0 0.0 0.05363122840059924 0.0 44-45 2.975380216399403E-4 0.0 0.0 0.06657413234193665 0.0 46-47 2.975380216399403E-4 0.0 0.0 0.08301310803754335 0.0 48-49 2.975380216399403E-4 0.0 0.0 0.09662547252757062 0.0 50-51 2.975380216399403E-4 0.0 0.0 0.11306444822317732 0.0 52-53 2.975380216399403E-4 0.0 0.0 0.16223260629917746 0.0 54-55 2.975380216399403E-4 0.0 0.0 0.2383279553335922 0.0 56-57 2.975380216399403E-4 0.0 0.0 0.29634786955338055 0.0 58-59 2.975380216399403E-4 0.0 0.0 0.3913368829619315 0.0 60-61 3.719225270499254E-4 0.0 0.0 0.5261959912702345 0.0 62-63 4.4630703245991047E-4 0.0 0.0 0.6100273288672876 0.0 64-65 4.4630703245991047E-4 0.0 0.0 0.6754856936280744 0.0 66-67 4.4630703245991047E-4 0.0 0.0 0.8734228625240448 0.0 68-69 4.4630703245991047E-4 0.0 0.0 1.0533589811107986 0.0 70-71 4.4630703245991047E-4 0.0 0.0 1.1455957678191802 0.0 72-73 4.4630703245991047E-4 0.0 0.0 1.3433841677043306 0.0 74-75 4.4630703245991047E-4 0.0 0.0 1.629243821994903 1.4876901081997015E-4 76-77 4.4630703245991047E-4 0.0 0.0 1.9360799068110914 1.4876901081997015E-4 78-79 4.4630703245991047E-4 0.0 0.0 2.2150218020985353 1.4876901081997015E-4 80-81 4.4630703245991047E-4 0.0 0.0 2.5170972785684853 1.4876901081997015E-4 82-83 4.4630703245991047E-4 0.0 0.0 2.8087589242810367 1.4876901081997015E-4 84-85 4.4630703245991047E-4 0.0 0.0 3.1461670408207287 1.4876901081997015E-4 86-87 4.4630703245991047E-4 0.0 0.0 3.742953927725039 1.4876901081997015E-4 88-89 4.4630703245991047E-4 0.0 0.0 4.419704157945084 1.4876901081997015E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAG 40 7.500712E-6 59.430103 1 GGTATCA 4550 0.0 28.108479 1 GTATCAA 6445 0.0 28.105965 1 TAAGGGT 155 4.5634806E-8 27.59186 4 CCGTCTA 70 0.008387724 27.143793 9 CTACACG 150 1.0552685E-6 25.343634 4 GTACATG 3985 0.0 25.173904 1 TACCTGG 630 0.0 24.89107 2 TACATGG 4095 0.0 24.252836 2 ACATGGG 4090 0.0 23.120644 3 ATCAACG 7835 0.0 23.107668 3 GAGTACT 4285 0.0 22.947113 12-13 GGGGCGC 145 2.3331988E-5 22.930119 6 TCAACGC 7920 0.0 22.859669 4 CAACGCA 7940 0.0 22.802088 5 AGTACTT 4425 0.0 22.650497 12-13 TATCAAC 7980 0.0 22.568695 2 AACGCAG 8070 0.0 22.54247 6 GTACTTT 4480 0.0 21.840647 14-15 GTACCTG 765 0.0 21.130703 1 >>END_MODULE