FastQCFastQC Report
Thu 26 May 2016
SRR937284_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937284_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738220
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39880.5402183630895939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30460.41261412587033675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18910.2561567012543686No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11620.15740565143182247No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10040.13600281758825283No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8300.11243260816558749No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.10457587169136573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53200.032.2404141
TACCTGG6350.031.423252
GTACATG44350.030.1035521
CGTATCC653.042769E-729.22537294-95
TACATGG44350.029.1374032
GTACCTG7400.027.6084331
ACATGGG44950.027.1630783
TAGGTCG700.00837915527.1498225
GGTATCA35250.026.957271
TATCAAC64350.026.7260972
ATCAACG64250.026.6937523
TCAACGC64900.026.4281984
CAACGCA65900.026.0992585
AACGCAG66500.025.8567566
CGCCGTA651.0040589E-525.57133390-91
GAGTACT33050.024.35879912-13
CCGTATC701.7829376E-523.74480892-93
ACGCAGA72200.023.5522777
CGCAGAG72350.023.4362058
CATGGGG13400.023.0469574
Produced by FastQC (version 0.11.5)