##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937284_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 738220 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.873958982417165 33.0 31.0 34.0 30.0 34.0 2 31.972854975481564 33.0 31.0 34.0 30.0 34.0 3 32.01701118907643 34.0 31.0 34.0 30.0 34.0 4 35.616434125328496 37.0 35.0 37.0 33.0 37.0 5 35.63435290292867 37.0 35.0 37.0 33.0 37.0 6 35.679254151878844 37.0 35.0 37.0 35.0 37.0 7 35.56800682723308 37.0 35.0 37.0 33.0 37.0 8 35.58325837826122 37.0 35.0 37.0 33.0 37.0 9 37.22383435832137 39.0 37.0 39.0 34.0 39.0 10-11 37.121759773509254 39.0 37.0 39.0 34.0 39.0 12-13 36.98573189564087 39.0 37.0 39.0 33.0 39.0 14-15 38.17193248625071 40.0 38.0 41.0 33.0 41.0 16-17 38.111983555037796 40.0 38.0 41.0 33.0 41.0 18-19 38.11105632467286 40.0 38.0 41.0 33.5 41.0 20-21 38.01485600498496 40.0 38.0 41.0 33.0 41.0 22-23 37.86062826799599 40.0 38.0 41.0 33.0 41.0 24-25 37.79951775893365 40.0 37.5 41.0 33.0 41.0 26-27 37.81978136598846 40.0 38.0 41.0 33.0 41.0 28-29 37.74605334453144 40.0 38.0 41.0 33.0 41.0 30-31 37.53648709056921 40.0 37.0 41.0 32.5 41.0 32-33 37.292194738695784 40.0 36.5 41.0 32.0 41.0 34-35 37.31883584839208 40.0 37.0 41.0 32.0 41.0 36-37 37.201529354393 40.0 37.0 41.0 31.5 41.0 38-39 37.09068570344884 40.0 36.0 41.0 31.0 41.0 40-41 36.886499959361714 39.5 36.0 41.0 31.0 41.0 42-43 36.61148641326434 39.0 36.0 41.0 30.0 41.0 44-45 36.35540286093577 39.0 36.0 41.0 30.0 41.0 46-47 35.98194305220666 39.0 35.0 41.0 29.5 41.0 48-49 35.62965782558045 39.0 35.0 41.0 27.5 41.0 50-51 34.68160304516269 38.0 34.0 39.5 25.5 40.5 52-53 34.80717062664246 38.0 34.5 39.5 26.0 40.5 54-55 35.189157026360704 39.0 35.0 40.0 26.0 41.0 56-57 34.989094714312806 39.0 35.0 40.0 26.0 41.0 58-59 34.744758337622926 38.0 34.5 40.0 25.0 41.0 60-61 34.43524694535505 38.0 34.0 40.0 23.5 41.0 62-63 34.13255669041749 38.0 34.0 40.0 23.0 41.0 64-65 33.72484557448999 37.0 33.5 40.0 22.0 41.0 66-67 33.37924941074476 37.0 33.0 39.5 21.5 41.0 68-69 32.89995123404947 36.0 32.5 39.0 19.5 41.0 70-71 32.43004659857495 36.0 32.0 39.0 18.0 40.0 72-73 31.968650266858116 35.0 31.5 38.5 14.5 40.0 74-75 31.475293273008045 35.0 31.0 37.5 9.5 39.5 76-77 30.980133293598115 35.0 31.0 37.0 7.0 39.0 78-79 30.469084419278808 34.5 30.0 36.5 4.0 39.0 80-81 29.953076996017447 34.0 30.0 36.0 2.0 38.0 82-83 29.460687193519547 34.0 29.0 35.5 2.0 37.0 84-85 28.919549050418574 34.0 29.0 35.0 2.0 37.0 86-87 28.4456246105497 34.0 28.5 35.0 2.0 36.0 88-89 27.98068800628539 34.0 27.0 35.0 2.0 36.0 90-91 27.473207851318037 33.0 26.5 35.0 2.0 35.5 92-93 26.96358673566146 33.0 25.5 35.0 2.0 35.0 94-95 26.42121386578527 33.0 25.0 35.0 2.0 35.0 96-97 25.69656606431687 32.0 23.0 34.5 2.0 35.0 98-99 24.76106512963615 32.0 10.5 34.0 2.0 35.0 100-101 22.174337595838637 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.04941227634376233 1101 2 -0.08764733342646736 1101 3 -0.1780828049312504 1101 4 -0.11087606508522896 1101 5 -0.04505815247627254 1101 6 -0.17145594892633653 1101 7 0.013185977393114001 1101 8 -0.20469122558137087 1101 9 -0.05524853147397124 1101 10-11 -0.06446438929836518 1101 12-13 -0.05696322782224428 1101 14-15 -0.09898751660494298 1101 16-17 -0.12923192429295227 1101 18-19 -0.020584143925042042 1101 20-21 0.14088637071638743 1101 22-23 -0.07881080611752367 1101 24-25 0.09523177397984028 1101 26-27 0.04566284543227539 1101 28-29 -0.0750831851329039 1101 30-31 0.01612941435744375 1101 32-33 -0.23142472288039784 1101 34-35 -0.025145700312044994 1101 36-37 0.13790414532441986 1101 38-39 -0.050361265815162426 1101 40-41 -0.03327002821794878 1101 42-43 0.09598401397362721 1101 44-45 0.28863143115442114 1101 46-47 0.37246678739349903 1101 48-49 0.21009273886483015 1101 50-51 0.023606840648874083 1101 52-53 0.12107364965802958 1101 54-55 0.1602870996745409 1101 56-57 0.2945020667933633 1101 58-59 0.34661812915225454 1101 60-61 0.25490413833292536 1101 62-63 0.15068148236707657 1101 64-65 0.041546379903515174 1101 66-67 0.15816363188256588 1101 68-69 0.08080283750481243 1101 70-71 -0.04255925601819399 1101 72-73 0.05352700036544533 1101 74-75 -0.21874165379054133 1101 76-77 -0.1058160972265405 1101 78-79 -0.052941191462341664 1101 80-81 0.05641871186554681 1101 82-83 0.00722952071813765 1101 84-85 -0.06885911726996596 1101 86-87 -0.05139968944023465 1101 88-89 -0.3014904316134519 1101 90-91 -0.4750572482805886 1101 92-93 -0.3451786870845055 1101 94-95 -0.4521959464890024 1101 96-97 -0.23040903401722446 1101 98-99 0.1058062874619381 1101 100-101 0.4364599144975365 1102 1 -0.38769358919272534 1102 2 -0.382094374567302 1102 3 -0.3556153731047118 1102 4 -0.4583588479698406 1102 5 -0.35858632968705706 1102 6 -0.3448348211861898 1102 7 -0.3012617358338474 1102 8 -0.4463448833082708 1102 9 -0.39171724841064304 1102 10-11 -0.33045672955316263 1102 12-13 -0.3978101785369432 1102 14-15 -0.23009064414754477 1102 16-17 -0.3106478264616541 1102 18-19 -0.3806308640938383 1102 20-21 -0.3250556649102876 1102 22-23 -0.376644754002335 1102 24-25 -0.38621355741462793 1102 26-27 -0.5734267426298487 1102 28-29 -0.5888858993425359 1102 30-31 -0.6073149862995777 1102 32-33 -0.8139737011567476 1102 34-35 -0.7814813849284974 1102 36-37 -0.7066066930826551 1102 38-39 -0.7225879176959396 1102 40-41 -0.6676236086549565 1102 42-43 -0.7319996156376689 1102 44-45 -0.8640130749997539 1102 46-47 -0.8786159331704013 1102 48-49 -1.1471795894146197 1102 50-51 -1.0836250097162434 1102 52-53 -1.1355542905914788 1102 54-55 -1.0494694524014463 1102 56-57 -1.1650337660893726 1102 58-59 -1.1770067164461508 1102 60-61 -1.0412439935222295 1102 62-63 -1.0057577646113316 1102 64-65 -1.147450857415187 1102 66-67 -1.2417423778911925 1102 68-69 -0.9846344207096536 1102 70-71 -1.2007097768340245 1102 72-73 -0.8792374018801361 1102 74-75 -1.136193693826506 1102 76-77 -1.1854964897248372 1102 78-79 -0.9944722443870901 1102 80-81 -1.0726182599995013 1102 82-83 -0.9631513382690287 1102 84-85 -0.8312741263787693 1102 86-87 -0.8199092977260385 1102 88-89 -0.978157943479335 1102 90-91 -0.9853676604221313 1102 92-93 -1.0995444465367328 1102 94-95 -1.1160291591523297 1102 96-97 -0.8667707263300883 1102 98-99 -0.9633264261265246 1102 100-101 -0.8931703261194812 1103 1 -0.045100272031756816 1103 2 -0.2547687817979174 1103 3 -0.16155064777409223 1103 4 -0.13139297622713997 1103 5 -0.19613230355042788 1103 6 -0.11444340753879345 1103 7 -0.06697110276396501 1103 8 -0.10809462898477307 1103 9 -0.050519042369479905 1103 10-11 -0.039266715663188734 1103 12-13 -0.1684623471338611 1103 14-15 -0.24926700282193082 1103 16-17 -0.20705012711115955 1103 18-19 -0.22713685047775556 1103 20-21 -0.26404981546979656 1103 22-23 -0.2596039790982019 1103 24-25 -0.5813793323187681 1103 26-27 -0.4989484726165472 1103 28-29 -0.2589298924171217 1103 30-31 -0.4111734895079664 1103 32-33 -0.42544687002754955 1103 34-35 -0.37087303822688256 1103 36-37 -0.5165237200409436 1103 38-39 -0.5971911485825458 1103 40-41 -0.3474315455044703 1103 42-43 -0.6711900110129037 1103 44-45 -0.7046659527679608 1103 46-47 -0.350677029500325 1103 48-49 -0.37604628477419766 1103 50-51 -0.3486728910058545 1103 52-53 -0.378573232801358 1103 54-55 -0.20700146292652022 1103 56-57 -0.14464020047390846 1103 58-59 -0.15144965016552447 1103 60-61 -0.12982193389314745 1103 62-63 -0.07281785831976606 1103 64-65 -0.3048685446364132 1103 66-67 -0.35674550802657023 1103 68-69 -0.4619965724821 1103 70-71 -0.5587587487801855 1103 72-73 -0.03158695974851966 1103 74-75 -0.13343098254237162 1103 76-77 -0.2627356369585776 1103 78-79 -0.26892520432135925 1103 80-81 -0.34640117532934056 1103 82-83 -0.2253570321809164 1103 84-85 -0.07774661053246135 1103 86-87 0.1416737926957481 1103 88-89 -0.018393632891651635 1103 90-91 0.03639350535766184 1103 92-93 -0.06193330227661775 1103 94-95 -0.4816448665629238 1103 96-97 -0.3611010240842134 1103 98-99 0.1163685323991821 1103 100-101 0.16107455317467512 1104 1 -0.05710777672218725 1104 2 -0.04267919455589109 1104 3 -0.2075110136277516 1104 4 -0.15325625014833832 1104 5 -0.11986588704010614 1104 6 -0.05577197226675423 1104 7 -0.07372532415233479 1104 8 -0.13858243386282254 1104 9 -0.09747612492107294 1104 10-11 -0.1618892531866436 1104 12-13 -0.14620918109258696 1104 14-15 -0.13717448406020338 1104 16-17 -0.14218145919370784 1104 18-19 -0.09411621257905978 1104 20-21 -0.1770071325429683 1104 22-23 -0.3269790963589898 1104 24-25 -0.07551336139943743 1104 26-27 0.025034290047621255 1104 28-29 -0.09156063819572324 1104 30-31 -0.17460278125280126 1104 32-33 -0.059669431003158024 1104 34-35 0.012502313555486921 1104 36-37 -0.1109978054067966 1104 38-39 0.07517341520732401 1104 40-41 0.15263294792892879 1104 42-43 0.19205752651055263 1104 44-45 0.028161204830531972 1104 46-47 0.0957699686235074 1104 48-49 0.01900530997252048 1104 50-51 -0.19824488912968974 1104 52-53 -0.2370423932141108 1104 54-55 -0.1258130248402196 1104 56-57 -0.047155444376350886 1104 58-59 -0.15924749256702597 1104 60-61 -0.1625376937430545 1104 62-63 0.1637469868960011 1104 64-65 0.4513873184029862 1104 66-67 0.3982732536995073 1104 68-69 0.23601292564173093 1104 70-71 0.3038438544093012 1104 72-73 0.3030753291820609 1104 74-75 0.22084610469965682 1104 76-77 0.3174443583509934 1104 78-79 0.4019765311383061 1104 80-81 0.3836703537513344 1104 82-83 0.38552183742508817 1104 84-85 0.3447264194863031 1104 86-87 0.36192766230175266 1104 88-89 -0.14183992745075358 1104 90-91 -0.014477155993180446 1104 92-93 0.19507009828623367 1104 94-95 -0.016266114217707184 1104 96-97 0.05264780330790586 1104 98-99 0.11458030538229735 1104 100-101 0.7163190419176431 1105 1 -0.07484243529269818 1105 2 -0.09630550481064759 1105 3 -0.07655847113987946 1105 4 -0.11862651372580046 1105 5 -0.11246909610418498 1105 6 -0.1492724302198738 1105 7 9.651334615625728E-4 1105 8 0.02890119855347706 1105 9 -0.04148372355060559 1105 10-11 0.011706357247902588 1105 12-13 -0.11978613417782924 1105 14-15 -0.33758494574478703 1105 16-17 -0.3233307519105608 1105 18-19 -0.19193839107901312 1105 20-21 -0.15656546220136391 1105 22-23 -0.05236920457161176 1105 24-25 -0.2035350039246211 1105 26-27 -0.18331996213190394 1105 28-29 -0.09064812420271551 1105 30-31 -0.3542493092897061 1105 32-33 -0.35770956126538067 1105 34-35 -0.21167506695267235 1105 36-37 -0.08629293075026112 1105 38-39 0.018513589568733835 1105 40-41 -0.07634021941053959 1105 42-43 0.06971365460173473 1105 44-45 -0.011253293625991034 1105 46-47 -0.307645174359628 1105 48-49 -0.4105292739685851 1105 50-51 -0.575705963114558 1105 52-53 -0.645466186422837 1105 54-55 -0.6548548851475573 1105 56-57 -0.8663674257868408 1105 58-59 -0.5364227698830817 1105 60-61 -0.7718527994114979 1105 62-63 -0.7013993884898753 1105 64-65 -0.5196149204789293 1105 66-67 -0.6090590845752004 1105 68-69 -0.5686191853525386 1105 70-71 -0.8775166055791601 1105 72-73 -0.811048284237657 1105 74-75 -0.6411598995745749 1105 76-77 -0.775429382277931 1105 78-79 -0.7253166267028668 1105 80-81 -0.6508253889807776 1105 82-83 -0.5355058432967823 1105 84-85 -0.3215631425748242 1105 86-87 -0.279545081007047 1105 88-89 -0.23594505313344172 1105 90-91 -0.17306123279361785 1105 92-93 -0.38269882196208016 1105 94-95 -0.3810427348629126 1105 96-97 -0.1115105806264225 1105 98-99 0.06755106422757606 1105 100-101 0.8513219253261681 1106 1 -0.2972572777432667 1106 2 -0.1966093760377987 1106 3 -0.09201569516745778 1106 4 -0.06078331831001549 1106 5 -0.07631215529426072 1106 6 -0.21568026803217322 1106 7 -0.35266802827131727 1106 8 -0.22578815189156387 1106 9 -0.3105844360989778 1106 10-11 -0.8227772044832165 1106 12-13 -0.4726656601123551 1106 14-15 -0.5260235573850593 1106 16-17 -0.23926850115417864 1106 18-19 -0.33741756502386977 1106 20-21 -0.20485747739712679 1106 22-23 -0.14181456817342308 1106 24-25 -0.14711009395444563 1106 26-27 -0.4580707459790432 1106 28-29 -0.3271480462025096 1106 30-31 -0.2330391855220597 1106 32-33 -0.4899462776104855 1106 34-35 -0.28540833261258314 1106 36-37 -0.5307787555859633 1106 38-39 -0.23918041671962698 1106 40-41 -0.3482840944168544 1106 42-43 -0.3209091825404258 1106 44-45 -0.14493246449477937 1106 46-47 -0.06266739892551954 1106 48-49 -0.15441394791674412 1106 50-51 -0.441308311470074 1106 52-53 -0.1813638589406139 1106 54-55 -0.47249259945586886 1106 56-57 -0.42695161437595885 1106 58-59 -0.16401572745316884 1106 60-61 -0.2678627043391302 1106 62-63 -0.5167157727079044 1106 64-65 -0.39684698976947885 1106 66-67 -0.36557100322647074 1106 68-69 -0.3415792602327272 1106 70-71 -0.05342794461200384 1106 72-73 -0.24891288027349034 1106 74-75 -0.041417410115585085 1106 76-77 0.15916689524460637 1106 78-79 0.20385588997929105 1106 80-81 -0.10547540974421565 1106 82-83 -0.0524747332989115 1106 84-85 -0.13078122895511157 1106 86-87 -0.20719055139223386 1106 88-89 -0.22369462632949677 1106 90-91 -0.03701019648946513 1106 92-93 -0.13864276727844427 1106 94-95 0.224582727920005 1106 96-97 0.4266864500924754 1106 98-99 0.3398673627552391 1106 100-101 0.8214610293564704 1107 1 -0.1416606222506438 1107 2 -0.13089057666971016 1107 3 -0.14938093232693817 1107 4 -0.02276844527028743 1107 5 -0.3731179195604355 1107 6 -0.18991831373016765 1107 7 -0.11762776317672774 1107 8 -0.28614340947257944 1107 9 -0.1836549867096906 1107 10-11 -0.19258031009721321 1107 12-13 -0.09234739247470003 1107 14-15 -0.0835159474748366 1107 16-17 -0.1836448738278591 1107 18-19 -0.1534658060749976 1107 20-21 -0.03960875855922552 1107 22-23 -0.12596283619638626 1107 24-25 -0.13526945896041553 1107 26-27 -0.2650063603588819 1107 28-29 -0.059040312992472366 1107 30-31 -0.19004993572434614 1107 32-33 -0.11190520336088383 1107 34-35 -0.23648142628191238 1107 36-37 -0.36079455337427646 1107 38-39 -0.02624934902031839 1107 40-41 -0.06606623906294118 1107 42-43 -0.5183083271479845 1107 44-45 -0.42631033988491396 1107 46-47 -0.3584983240594184 1107 48-49 -0.32611664124699047 1107 50-51 -0.5500741574223724 1107 52-53 -0.6372456452466295 1107 54-55 -0.7983740004499751 1107 56-57 -0.7995944687665926 1107 58-59 -0.8726621267133723 1107 60-61 -0.6218872872389909 1107 62-63 -0.861862769201565 1107 64-65 -0.6297102425726209 1107 66-67 -0.6582308113411486 1107 68-69 -0.4814667491550111 1107 70-71 -0.4183106171354112 1107 72-73 -0.39164011797240406 1107 74-75 -0.3106579603409969 1107 76-77 -0.39658003189291335 1107 78-79 -0.14660070463405006 1107 80-81 -0.4968174511267165 1107 82-83 -0.3972017398106864 1107 84-85 -0.16950122279012092 1107 86-87 -0.4126658336332092 1107 88-89 0.06283486929721604 1107 90-91 0.06275861636454394 1107 92-93 -0.12566160903083912 1107 94-95 0.04348998334326737 1107 96-97 -0.1264172373425012 1107 98-99 0.3082794923741652 1107 100-101 0.8045304240314621 1108 1 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-1.3655888749523797 2316 50-51 -1.2797558440697472 2316 52-53 -1.4919376667431123 2316 54-55 -1.3565729807448648 2316 56-57 -1.3159855394569604 2316 58-59 -1.2767891764584434 2316 60-61 -1.2374653497992796 2316 62-63 -1.2670370836565752 2316 64-65 -1.2076350048710935 2316 66-67 -1.3861349039413895 2316 68-69 -1.369058040322166 2316 70-71 -1.5385807011921813 2316 72-73 -1.65158913271679 2316 74-75 -1.2901053904085131 2316 76-77 -0.942266954863836 2316 78-79 -0.8984816471722894 2316 80-81 -0.9690856060116531 2316 82-83 -1.3698763987606632 2316 84-85 -1.4170759781986924 2316 86-87 -1.469741760324677 2316 88-89 -1.248857289997261 2316 90-91 -0.8624446356679805 2316 92-93 -1.3491148266138637 2316 94-95 -0.7599274067628379 2316 96-97 -0.5823838902293694 2316 98-99 -0.609599796588217 2316 100-101 -0.14009545443071403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2792.0 3 654.0 4 469.0 5 487.0 6 574.0 7 700.0 8 857.0 9 1057.0 10 1336.0 11 1755.0 12 2385.0 13 3210.0 14 3594.0 15 4631.0 16 6159.0 17 8626.0 18 7451.0 19 4649.0 20 4545.0 21 4761.0 22 5128.0 23 5671.0 24 6342.0 25 7244.0 26 8326.0 27 9632.0 28 11514.0 29 13829.0 30 16996.0 31 21187.0 32 27468.0 33 36078.0 34 50028.0 35 70937.0 36 104417.0 37 148092.0 38 120374.0 39 14264.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.399408043011924 19.97732641811714 15.029462079452577 21.59380345941836 2 14.6653436424251 20.818220812926906 41.52228327559808 22.99415226904992 3 18.998847674514877 26.32161146407771 30.194999531740528 24.484541329666882 4 11.816757792775428 17.049767343370778 38.99794762646802 32.13552723738577 5 12.552427903996874 38.60906225652413 34.47957585316658 14.358933986312413 6 29.57272824777987 37.245725721159396 18.547783810350047 14.633762220710686 7 27.212099818068264 33.933527031797325 21.397480109702123 17.45689304043229 8 26.007499056509005 34.72046873175804 20.279762050212728 18.992270161520228 9 26.40837087948263 16.8336616217493 21.16937646015945 35.58859103860862 10-11 23.96930853233934 27.610441289975718 27.876069249270085 20.54418092841486 12-13 25.297455598044095 25.254144895344915 28.450379715384873 20.99801979122612 14-15 22.262721205901357 26.261464153648166 27.441817214285326 24.03399742616515 16-17 20.605232020994883 29.833598190508454 27.994849031410183 21.566320757086483 18-19 20.516014210719945 27.97121202453888 31.060496017658867 20.45227774708231 20-21 21.756125438888823 26.876998458586478 30.853791056000617 20.513085046524083 22-23 21.212520217380533 26.975534169833466 30.875936040115082 20.936009572670923 24-25 20.98208650114632 26.99248140712658 31.122617268466296 20.902814823260808 26-27 20.686238043912066 27.30026579642546 31.082977897357512 20.93051826230496 28-29 20.348622919106045 27.485414651529805 31.27010470226883 20.89585772709532 30-31 20.742544820676567 27.12686090996223 31.12345608619037 21.00713818317083 32-33 20.515417312080935 27.329091360793477 30.944655698357472 21.21083562876812 34-35 21.016167692718124 27.36315976330558 30.78778893547925 20.83288360849705 36-37 20.89612310290226 27.04522763815446 31.005131776221706 21.053517482721574 38-39 20.628827172979054 27.476098621776558 30.910458235879418 20.98461596936497 40-41 20.771330080488564 27.479185571649346 30.860804804201504 20.888679543660587 42-43 20.678088504993493 27.44757072132425 30.49355264783299 21.380788125849275 44-45 20.802526290637466 27.512103332455396 30.337074075381427 21.348296301525714 46-47 21.017831880154127 27.82141237614487 29.8741015496656 21.286654194035403 48-49 21.002870912140857 27.95375041325793 29.636380546226842 21.406998128374376 50-51 20.939501363829528 28.081311122847847 29.61872512827058 21.36046238505205 52-53 21.05546547598678 28.467610736760342 29.086172197733255 21.390751589519628 54-55 20.908878612528408 28.060211748541143 29.36001596667635 21.6708936722541 56-57 21.015870344114234 28.372575382946795 29.04049676355746 21.571057509381507 58-59 21.194228170885555 27.977681428312586 29.358137476918316 21.469952923883547 60-61 21.00668690698997 28.147168956030903 29.704484041405347 21.141660095573776 62-63 20.956485917323448 28.14859003567752 29.641317781003707 21.25360626599533 64-65 20.76132386802037 28.15855960032329 29.59518618124025 21.484930350416093 66-67 20.747553069698917 28.25022852493348 29.201652301434393 21.800566103933207 68-69 20.716376868441877 28.226519014572933 29.418127873155647 21.638976243829543 70-71 20.898360491085818 28.221418871827307 29.27058513753511 21.609635499551764 72-73 20.84348788613599 28.087238552077842 29.246740872100478 21.82253268968569 74-75 20.60487644647982 28.49525378845205 29.10767693864435 21.79219282642378 76-77 20.728216029548907 28.17747805347527 29.178730977076796 21.91557493989903 78-79 20.870277563608326 28.03849891950004 29.02472661722067 22.066496899670963 80-81 20.770793800747626 28.35996438334596 28.92936935176061 21.93987246414581 82-83 20.86022512416033 28.270618623167454 29.13841756147616 21.730738691196052 84-85 20.681433964876373 28.17362422725505 29.05541367686451 22.089528131004066 86-87 20.673649575439782 28.28980722909556 28.947330913997288 22.089212281467375 88-89 20.860562419619193 27.942149573621183 28.839632021337604 22.357655985422017 90-91 20.574429296885075 27.812724402351634 29.25236093332374 22.36048536743955 92-93 20.807023449504026 27.70885478030003 29.214422612293205 22.26969915790274 94-95 20.485773193637403 27.5925254803914 29.385891688146344 22.53580963782486 96-97 20.346988488240846 27.593335790297434 29.52558195095167 22.53409377051005 98-99 20.365641929909582 27.778320756586673 29.09266883586701 22.76336847763674 100-101 20.463544745151502 27.46985106551909 29.477042971604934 22.589561217724473 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 982.0 1 594.0 2 214.0 3 222.5 4 197.5 5 186.5 6 290.0 7 546.5 8 887.5 9 1153.0 10 1130.0 11 1064.5 12 1151.0 13 1253.0 14 1324.0 15 1374.0 16 1434.5 17 1491.5 18 1582.5 19 1710.5 20 1899.5 21 2116.5 22 2410.0 23 2843.5 24 3365.5 25 4106.0 26 5143.0 27 6338.0 28 7524.5 29 8865.5 30 10526.0 31 12256.0 32 14028.5 33 16086.5 34 18488.0 35 20269.0 36 22275.5 37 24656.5 38 25577.5 39 25623.0 40 25557.5 41 25996.0 42 26436.0 43 26815.5 44 27812.0 45 28740.0 46 28349.0 47 28173.5 48 30193.5 49 29494.5 50 25940.5 51 23283.0 52 21174.5 53 19840.0 54 18506.5 55 16724.5 56 14815.5 57 12882.5 58 11342.5 59 9765.0 60 8048.5 61 6513.5 62 5111.0 63 3825.0 64 2725.5 65 2012.5 66 1499.0 67 1092.0 68 786.5 69 539.0 70 386.0 71 283.0 72 210.5 73 174.5 74 135.5 75 103.0 76 75.0 77 51.5 78 29.5 79 20.0 80 18.5 81 12.5 82 8.5 83 5.5 84 3.0 85 2.0 86 1.0 87 0.5 88 1.0 89 1.5 90 2.0 91 2.5 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22757443580504458 2 0.20644252390886186 3 0.19628295088185096 4 0.2048169922245401 5 0.20237869469805747 6 0.22527159925225543 7 0.22757443580504458 8 0.2159247920674054 9 0.21280918967245535 10-11 0.22533932973910217 12-13 0.22777762726558481 14-15 0.21348649454092278 16-17 0.22479748584432824 18-19 0.2157893310937119 20-21 0.21111592750128688 22-23 0.20854216900111078 24-25 0.20495245319823358 26-27 0.21213188480398798 28-29 0.21775351521226732 30-31 0.21917585543604887 32-33 0.2184985505675815 34-35 0.22466202487063475 36-37 0.2080003251063369 38-39 0.20955812630381188 40-41 0.20745848121156296 42-43 0.21145457993552058 44-45 0.2229687626994663 46-47 0.22676166996288372 48-49 0.2295386199236 50-51 0.22906450651567287 52-53 0.2484354257538403 54-55 0.22838720164720544 56-57 0.20745848121156296 58-59 0.1943187667632955 60-61 0.19059358998672482 62-63 0.200685432526889 64-65 0.19296415702636072 66-67 0.19364146189482812 68-69 0.19547018503969005 70-71 0.19567337650023028 72-73 0.200685432526889 74-75 0.19973720571103468 76-77 0.20901628240903797 78-79 0.20644252390886186 80-81 0.20129500690850965 82-83 0.1992630923031075 84-85 0.20041451057950205 86-87 0.20542656660616077 88-89 0.20217550323751726 90-91 0.20874536046165101 92-93 0.2015659288558966 94-95 0.20007585814526835 96-97 0.2084067080274173 98-99 0.20901628240903797 100-101 0.19228685215789332 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 738220.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.3356801734833 #Duplication Level Percentage of deduplicated Percentage of total 1 84.75493247623184 57.07031026344721 2 9.380235957186718 12.632491367298657 3 2.4463965485008043 4.941893267020908 4 1.06322711775574 2.8637248461189992 5 0.5500537502767969 1.8519121703431725 6 0.3644488323246281 1.472424600780636 7 0.2500951618272007 1.1788229480812356 8 0.1686857809811189 0.9086857438367099 9 0.1421183956726018 0.861267495400098 >10 0.8097841384434349 9.991783465502992 >50 0.04923762228090646 2.238053287079361 >100 0.018766333228127534 2.042483201648774 >500 0.0010089426450425204 0.4391501929764554 >1k 0.0010089426450425204 1.5069971504646866 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3988 0.5402183630895939 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3046 0.41261412587033675 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1891 0.2561567012543686 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1162 0.15740565143182247 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1004 0.13600281758825283 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 830 0.11243260816558749 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 772 0.10457587169136573 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.354609736934789E-4 0.0 0.0 0.0 0.0 7 1.354609736934789E-4 0.0 0.0 0.0 0.0 8 1.354609736934789E-4 0.0 0.0 0.0 0.0 9 1.354609736934789E-4 0.0 0.0 0.0 0.0 10-11 1.354609736934789E-4 0.0 0.0 0.0 0.0 12-13 1.354609736934789E-4 0.0 0.0 0.0 0.0 14-15 1.354609736934789E-4 0.0 0.0 0.0 0.0 16-17 1.354609736934789E-4 0.0 0.0 0.0 0.0 18-19 1.354609736934789E-4 0.0 0.0 0.0 0.0 20-21 1.354609736934789E-4 0.0 0.0 0.0 0.0 22-23 1.354609736934789E-4 0.0 0.0 6.773048684673945E-5 6.773048684673945E-5 24-25 2.709219473869578E-4 0.0 0.0 2.709219473869578E-4 1.354609736934789E-4 26-27 2.709219473869578E-4 0.0 0.0 0.0010159573027010917 1.354609736934789E-4 28-29 2.709219473869578E-4 0.0 0.0 0.003454254829183712 1.354609736934789E-4 30-31 2.709219473869578E-4 0.0 0.0 0.008804963290076129 1.354609736934789E-4 32-33 2.709219473869578E-4 0.0 0.0 0.015374820514209856 2.709219473869578E-4 34-35 2.709219473869578E-4 0.0 0.0 0.02363793990951207 2.709219473869578E-4 36-37 2.709219473869578E-4 0.0 0.0 0.031968789791661026 2.709219473869578E-4 38-39 4.0638292108043675E-4 0.0 0.0 0.04029963967380998 2.709219473869578E-4 40-41 4.0638292108043675E-4 0.0 0.0 0.05188155292460242 2.709219473869578E-4 42-43 4.0638292108043675E-4 0.0 0.0 0.06441169299124921 2.709219473869578E-4 44-45 4.0638292108043675E-4 0.0 0.0 0.07504537942618732 2.709219473869578E-4 46-47 4.0638292108043675E-4 0.0 0.0 0.08920105117715585 2.709219473869578E-4 48-49 4.0638292108043675E-4 0.0 0.0 0.10681097775730812 2.709219473869578E-4 50-51 4.0638292108043675E-4 0.0 0.0 0.13139714448267453 2.709219473869578E-4 52-53 4.0638292108043675E-4 0.0 0.0 0.1870716046706944 2.709219473869578E-4 54-55 4.0638292108043675E-4 0.0 0.0 0.26712904012354044 2.709219473869578E-4 56-57 4.0638292108043675E-4 0.0 0.0 0.3445449865893636 2.709219473869578E-4 58-59 4.0638292108043675E-4 0.0 0.0 0.4527105740836065 2.709219473869578E-4 60-61 5.418438947739156E-4 0.0 0.0 0.5901357318956408 2.709219473869578E-4 62-63 5.418438947739156E-4 0.0 0.0 0.6838747256915283 2.709219473869578E-4 64-65 5.418438947739156E-4 0.0 0.0 0.7565495380780797 2.709219473869578E-4 66-67 5.418438947739156E-4 0.0 0.0 0.9489041207228197 2.709219473869578E-4 68-69 5.418438947739156E-4 0.0 0.0 1.142816504565035 2.709219473869578E-4 70-71 5.418438947739156E-4 0.0 0.0 1.2500338652434233 2.709219473869578E-4 72-73 5.418438947739156E-4 0.0 0.0 1.461759367126331 2.709219473869578E-4 74-75 5.418438947739156E-4 0.0 0.0 1.7569288288044214 2.709219473869578E-4 76-77 5.418438947739156E-4 0.0 0.0 2.0573812684565578 2.709219473869578E-4 78-79 5.418438947739156E-4 0.0 0.0 2.3397496681206142 2.709219473869578E-4 80-81 5.418438947739156E-4 0.0 0.0 2.654899623418493 2.709219473869578E-4 82-83 5.418438947739156E-4 0.0 0.0 2.951897808241446 2.709219473869578E-4 84-85 5.418438947739156E-4 0.0 0.0 3.285876838882718 2.709219473869578E-4 86-87 5.418438947739156E-4 0.0 0.0 3.8584703746850533 3.386524342336973E-4 88-89 5.418438947739156E-4 0.0 0.0 4.502451843623852 4.0638292108043675E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5320 0.0 32.240414 1 TACCTGG 635 0.0 31.42325 2 GTACATG 4435 0.0 30.103552 1 CGTATCC 65 3.042769E-7 29.225372 94-95 TACATGG 4435 0.0 29.137403 2 GTACCTG 740 0.0 27.608433 1 ACATGGG 4495 0.0 27.163078 3 TAGGTCG 70 0.008379155 27.149822 5 GGTATCA 3525 0.0 26.95727 1 TATCAAC 6435 0.0 26.726097 2 ATCAACG 6425 0.0 26.693752 3 TCAACGC 6490 0.0 26.428198 4 CAACGCA 6590 0.0 26.099258 5 AACGCAG 6650 0.0 25.856756 6 CGCCGTA 65 1.0040589E-5 25.571333 90-91 GAGTACT 3305 0.0 24.358799 12-13 CCGTATC 70 1.7829376E-5 23.744808 92-93 ACGCAGA 7220 0.0 23.552277 7 CGCAGAG 7235 0.0 23.436205 8 CATGGGG 1340 0.0 23.046957 4 >>END_MODULE