FastQCFastQC Report
Thu 26 May 2016
SRR937284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738220
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58230.7887892498171277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48180.6526509712551815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29950.4057056162119693No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16070.2176857847254206No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.18409146324943784No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.1079623960337027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67250.028.2690541
GGTATCA46700.028.0889661
TACCTGG6150.027.0315932
TCAACGC82700.022.8015174
ATCAACG83150.022.6781163
CGTTTAA1050.002188150322.632071
CAACGCA83650.022.6008785
TATCAAC83050.022.591042
AGTACTT46850.022.35667212-13
AACGCAG86300.021.9068766
GAGTACT45600.021.82364712-13
GTACATG47700.021.6214541
TACATGG48000.021.4732232
GTACCTG7750.020.8507061
GTACTTT47650.020.78365314-15
ACATGGG47100.020.2700063
CGCAGAG95150.019.8207158
AGAGTAC76800.019.76274510-11
ACGCAGA95450.019.7584177
CATGGGG15500.019.6122324
Produced by FastQC (version 0.11.5)