##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937284_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 738220 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37257321665628 33.0 31.0 34.0 31.0 34.0 2 32.565156728346565 34.0 31.0 34.0 31.0 34.0 3 32.606401885616755 34.0 31.0 34.0 31.0 34.0 4 36.221192869334345 37.0 35.0 37.0 35.0 37.0 5 36.171839018178865 37.0 35.0 37.0 35.0 37.0 6 36.23538240632874 37.0 36.0 37.0 35.0 37.0 7 36.187598547858364 37.0 36.0 37.0 35.0 37.0 8 36.1705128552464 37.0 36.0 37.0 35.0 37.0 9 37.90275527620493 39.0 38.0 39.0 35.0 39.0 10-11 37.83227222237274 39.0 38.0 39.0 35.0 39.0 12-13 37.745269025493755 39.0 37.0 39.0 35.0 39.0 14-15 38.956770339465194 40.0 38.0 41.0 36.0 41.0 16-17 38.932048034461275 40.0 38.0 41.0 36.0 41.0 18-19 38.96535856519736 40.0 38.0 41.0 36.0 41.0 20-21 38.90577199208908 40.0 38.0 41.0 35.0 41.0 22-23 38.76279835279456 40.0 38.0 41.0 35.0 41.0 24-25 38.696228766492375 40.0 38.0 41.0 34.5 41.0 26-27 38.65350234347484 40.0 38.0 41.0 35.0 41.0 28-29 38.5291647476362 40.0 38.0 41.0 34.0 41.0 30-31 38.56225583159492 40.0 38.0 41.0 35.0 41.0 32-33 38.46454850857468 40.0 38.0 41.0 35.0 41.0 34-35 38.330434016959714 40.0 38.0 41.0 34.0 41.0 36-37 38.161112540976944 40.0 38.0 41.0 34.0 41.0 38-39 38.03110793530384 40.0 37.5 41.0 34.0 41.0 40-41 37.844015334182224 40.0 37.0 41.0 33.0 41.0 42-43 37.602843325837824 40.0 37.0 41.0 33.0 41.0 44-45 37.25202717347132 40.0 37.0 41.0 33.0 41.0 46-47 36.789182086640835 40.0 37.0 41.0 31.5 41.0 48-49 36.36018666522175 40.0 36.0 41.0 30.0 41.0 50-51 36.09087738072661 39.5 36.0 41.0 29.5 41.0 52-53 35.86318103004525 39.0 36.0 41.0 29.0 41.0 54-55 35.64300750453794 39.0 35.0 40.5 28.0 41.0 56-57 35.38558966161849 39.0 35.0 40.0 28.0 41.0 58-59 35.13461298799815 38.5 35.0 40.0 27.0 41.0 60-61 34.818335997399146 38.0 34.5 40.0 26.0 41.0 62-63 34.465691799192655 38.0 34.0 40.0 26.0 41.0 64-65 34.09059223537699 37.5 34.0 40.0 25.5 41.0 66-67 33.68938256888191 37.0 33.5 39.5 23.5 41.0 68-69 33.27022161415296 36.0 33.0 39.0 22.5 40.5 70-71 32.86717441954973 36.0 33.0 39.0 22.0 40.0 72-73 32.39550946872206 35.0 32.0 38.0 20.5 40.0 74-75 31.91749613936225 35.0 32.0 37.5 19.0 39.5 76-77 30.66408455473978 34.0 30.5 36.0 18.0 38.5 78-79 31.246531521768578 35.0 31.0 36.0 18.0 39.0 80-81 31.028647286716698 35.0 31.0 36.0 17.0 38.0 82-83 30.68757281027336 34.5 31.0 36.0 12.0 37.0 84-85 30.319551082333177 34.0 31.0 35.0 7.5 37.0 86-87 29.930600633957358 34.0 31.0 35.0 4.5 36.0 88-89 29.473941372490586 34.0 30.0 35.0 2.0 36.0 90-91 29.003669637777357 34.0 29.0 35.0 2.0 35.5 92-93 28.68450597382894 34.0 29.0 35.0 2.0 35.0 94-95 28.267826664138063 34.0 29.0 35.0 2.0 35.0 96-97 27.81825607542467 34.0 29.0 35.0 2.0 35.0 98-99 27.110316030451628 33.0 27.0 35.0 2.0 35.0 100-101 24.661944271355424 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.005459811367018119 1101 2 -0.06194643991668869 1101 3 0.047038246789867344 1101 4 -0.011117641882030682 1101 5 0.003810298255508826 1101 6 -0.08475288731425223 1101 7 -0.036439188674101786 1101 8 0.042881368794390085 1101 9 0.14232699895209322 1101 10-11 -0.06817329461848942 1101 12-13 -0.1025360212170483 1101 14-15 -0.22911676317580287 1101 16-17 -0.029597573737142113 1101 18-19 -0.002213626232496324 1101 20-21 0.05742456182055378 1101 22-23 -0.08219922897787058 1101 24-25 0.019230664740426562 1101 26-27 0.08233089880786792 1101 28-29 -0.0890887592593188 1101 30-31 0.024325287798532713 1101 32-33 0.04484628355195497 1101 34-35 -0.09101641148595974 1101 36-37 -0.005596915405163827 1101 38-39 -0.2798309314848666 1101 40-41 0.10681929746945684 1101 42-43 -0.15267622511036194 1101 44-45 -0.03156874632337292 1101 46-47 -0.11838622953023048 1101 48-49 -0.05187894628949863 1101 50-51 -0.26295091495768474 1101 52-53 -0.048081073154513376 1101 54-55 -0.05827621953012141 1101 56-57 -0.08214331247206985 1101 58-59 -0.06782016297431426 1101 60-61 -0.1898119181359874 1101 62-63 -0.18326173032800597 1101 64-65 -0.32766515024799503 1101 66-67 -0.06007423479666585 1101 68-69 -0.12399740125078296 1101 70-71 -0.0555137443300211 1101 72-73 -0.07200532252131353 1101 74-75 -0.21342845950313816 1101 76-77 -0.15011466141755392 1101 78-79 -0.4943030240104882 1101 80-81 -0.29296566736351437 1101 82-83 -0.17130605308896207 1101 84-85 -0.18743355395344707 1101 86-87 -0.3772299012586835 1101 88-89 -0.3357673389901308 1101 90-91 -0.237686826669389 1101 92-93 -0.03454651711371426 1101 94-95 0.01825498801473202 1101 96-97 -0.1690575051736154 1101 98-99 -0.08836373782350293 1101 100-101 0.5174121088953321 1102 1 -0.17642426513920384 1102 2 -0.15541529025227163 1102 3 -0.16693259078218858 1102 4 -0.10730945933529767 1102 5 -0.09784272811776162 1102 6 -0.23661176552104735 1102 7 -0.24122994076902415 1102 8 -0.1637392905220949 1102 9 -0.2787746724038982 1102 10-11 -0.33345841697379797 1102 12-13 -0.2685392574135719 1102 14-15 -0.36796517415036334 1102 16-17 -0.42568796728429703 1102 18-19 -0.301143278163174 1102 20-21 -0.24312878908884983 1102 22-23 -0.1396442630251471 1102 24-25 -0.374107739788208 1102 26-27 -0.31396162973288 1102 28-29 -0.43673905451896644 1102 30-31 -0.3647468973061976 1102 32-33 -0.30022717240655084 1102 34-35 -0.3170425393432126 1102 36-37 -0.24355104450883402 1102 38-39 -0.3065244612302749 1102 40-41 -0.36805791952372857 1102 42-43 -0.3276146426733959 1102 44-45 -0.383988307288206 1102 46-47 -0.6176148579551963 1102 48-49 -0.47650906980060626 1102 50-51 -0.5226572133211533 1102 52-53 -0.5489675140206103 1102 54-55 -0.4612539683878296 1102 56-57 -0.6564785836537297 1102 58-59 -0.6572315479096034 1102 60-61 -0.6968784964131345 1102 62-63 -0.6594082736159592 1102 64-65 -0.7032136387161145 1102 66-67 -0.6944573462906689 1102 68-69 -0.46424762095166017 1102 70-71 -0.5569984436784097 1102 72-73 -0.5561960239042882 1102 74-75 -0.6856814856727453 1102 76-77 -0.6450526309231748 1102 78-79 -0.6339370629324179 1102 80-81 -0.7349967116326077 1102 82-83 -0.7175323404139249 1102 84-85 -0.7572953791560657 1102 86-87 -0.706140704228492 1102 88-89 -0.44683313520343404 1102 90-91 -0.6065694427544486 1102 92-93 -0.47160157827864424 1102 94-95 -0.2268018694531122 1102 96-97 -0.3090662830257287 1102 98-99 -0.12308004110542115 1102 100-101 0.46224249370536796 1103 1 -0.13885041350762606 1103 2 -0.04541548588573363 1103 3 -0.12203226603064365 1103 4 -0.024409780174167395 1103 5 -0.10064150932129934 1103 6 -0.1599711344075061 1103 7 -0.18898235684226705 1103 8 -0.2556503359873119 1103 9 -0.09681599019089049 1103 10-11 -0.14351666683685949 1103 12-13 -0.21861604354707254 1103 14-15 -0.2262617446957833 1103 16-17 -0.24467382787589287 1103 18-19 -0.36568948189585626 1103 20-21 -0.3084587493752551 1103 22-23 -0.21998725739089764 1103 24-25 -0.10710841472364763 1103 26-27 -0.18222572512375734 1103 28-29 -0.5404198980904553 1103 30-31 -0.4728936859829389 1103 32-33 -0.5816263429206785 1103 34-35 -0.48097848894803263 1103 36-37 -0.568982416290666 1103 38-39 -0.5358808515972839 1103 40-41 -0.5869020681269106 1103 42-43 -0.4409548883890224 1103 44-45 -0.6105612003158285 1103 46-47 -0.5774360245175245 1103 48-49 -0.4300118869224434 1103 50-51 -0.31565867079043386 1103 52-53 -0.43112036244379937 1103 54-55 -0.5653548125462109 1103 56-57 -0.5656508584796143 1103 58-59 -0.5553774317815794 1103 60-61 -0.6186397844638449 1103 62-63 -0.5832536693824508 1103 64-65 -0.8041837361415816 1103 66-67 -0.7594406685381045 1103 68-69 -0.8040091437625208 1103 70-71 -0.6542013742676573 1103 72-73 -0.5883612216897198 1103 74-75 -0.3081572026693813 1103 76-77 -0.2489737368391296 1103 78-79 -0.28392618082114396 1103 80-81 -0.43916896200430955 1103 82-83 -0.33488429479220017 1103 84-85 -0.2793120911444831 1103 86-87 -0.31039744380122514 1103 88-89 -0.333075945673734 1103 90-91 -0.3761437932513587 1103 92-93 -0.390307927562624 1103 94-95 -0.34493452361228094 1103 96-97 -0.05284513896124565 1103 98-99 0.05713933736707233 1103 100-101 0.6052126498208708 1104 1 -0.046343377860345925 1104 2 0.12322897940507715 1104 3 -0.05377476134021464 1104 4 0.026106167292994087 1104 5 -0.02130872320498156 1104 6 0.02678425958874442 1104 7 -0.04363174708616668 1104 8 0.08988202799017131 1104 9 0.05187068898359115 1104 10-11 -0.06590761430402381 1104 12-13 -0.11468780714932336 1104 14-15 -0.045111416365578805 1104 16-17 -0.033361093354315585 1104 18-19 -0.17940606257128167 1104 20-21 -0.02473747826831385 1104 22-23 -0.11511507164981083 1104 24-25 -0.0586386680069495 1104 26-27 -0.1430078545796718 1104 28-29 -0.10273228082967023 1104 30-31 -0.05513160812177631 1104 32-33 -0.0845813147780845 1104 34-35 0.01787235532963649 1104 36-37 0.028039159694323246 1104 38-39 0.14231743639521 1104 40-41 -0.010137293267618475 1104 42-43 0.0812308526991572 1104 44-45 0.14703729874608484 1104 46-47 0.11913116591399131 1104 48-49 0.15401719375297773 1104 50-51 0.007998693187047934 1104 52-53 0.1583613448732777 1104 54-55 0.2481448356714182 1104 56-57 0.22034820343407802 1104 58-59 0.08011740874374595 1104 60-61 0.09662757013277457 1104 62-63 0.20840615707158605 1104 64-65 0.20881462220739166 1104 66-67 0.3334357385913549 1104 68-69 0.43607059357331224 1104 70-71 0.44423905725204094 1104 72-73 0.2856743083290425 1104 74-75 0.24589122603606128 1104 76-77 0.36906976691321347 1104 78-79 0.18049982762163097 1104 80-81 0.15517027345535084 1104 82-83 0.20988001102393028 1104 84-85 0.4008913563226848 1104 86-87 0.46925194644267876 1104 88-89 0.36603873537692166 1104 90-91 0.2325510941782838 1104 92-93 0.3278469739383105 1104 94-95 0.42576493698809514 1104 96-97 0.5726708085059187 1104 98-99 0.5280557304252333 1104 100-101 1.2994258299334405 1105 1 -0.0625057307217034 1105 2 -0.08823002531014623 1105 3 -0.07859921264452652 1105 4 -0.044135360574301785 1105 5 -0.17725532926353083 1105 6 -0.10193279322851367 1105 7 -0.1016866336155644 1105 8 -0.14751069225135893 1105 9 -0.3117780100903289 1105 10-11 -0.14510975820306982 1105 12-13 -0.230033476948158 1105 14-15 -0.13842106051904324 1105 16-17 -0.13529297706814702 1105 18-19 -0.149414373572327 1105 20-21 -0.14844333667599585 1105 22-23 -0.33838587459428027 1105 24-25 -0.182304667532037 1105 26-27 -0.00768610011038362 1105 28-29 -0.09563146329854533 1105 30-31 -0.07479051785413304 1105 32-33 -0.141003683243774 1105 34-35 -0.20710050670773938 1105 36-37 -0.18766354392520412 1105 38-39 -0.12328774017115762 1105 40-41 -0.328960014526821 1105 42-43 -0.07357765505212654 1105 44-45 -0.23671130195272383 1105 46-47 -0.45354684215954677 1105 48-49 -0.23852665101787096 1105 50-51 -0.44250251106004157 1105 52-53 -0.2497486656426915 1105 54-55 -0.13021603138648885 1105 56-57 -0.3617017304171313 1105 58-59 -0.34577475177729866 1105 60-61 -0.39400220021221344 1105 62-63 -0.2450293393835139 1105 64-65 -0.3914660860934376 1105 66-67 -0.46615973615337936 1105 68-69 -0.4416669484057252 1105 70-71 -0.3629414780442701 1105 72-73 -0.42254849434404207 1105 74-75 -0.07042801817167899 1105 76-77 -0.09993443947099223 1105 78-79 -0.2941007354910923 1105 80-81 -0.42028004711183087 1105 82-83 -0.3134586368270824 1105 84-85 -0.35578967084920166 1105 86-87 -0.45523836869691436 1105 88-89 -0.22152444519229775 1105 90-91 -0.09610574628441526 1105 92-93 0.035426098060572286 1105 94-95 0.05220409706815232 1105 96-97 0.00827072709203236 1105 98-99 0.20967908942655455 1105 100-101 0.8278358845485911 1106 1 -0.20621506899431807 1106 2 -0.06708427917774884 1106 3 -0.07618450202529914 1106 4 -0.10311010828208111 1106 5 -0.19312123042957552 1106 6 -0.1806734524291329 1106 7 -0.1445757612656493 1106 8 -0.09767625043621564 1106 9 -0.14490810344888416 1106 10-11 -0.15809734188850655 1106 12-13 -0.31899655758279977 1106 14-15 -0.20577588675435976 1106 16-17 -0.12691289854061694 1106 18-19 -0.06859517365670342 1106 20-21 -0.26519426553760894 1106 22-23 -0.2626355895090171 1106 24-25 -0.23990287638363128 1106 26-27 -0.3005021712289917 1106 28-29 -0.23126173792518756 1106 30-31 -0.25970044409449855 1106 32-33 -0.2882735661555742 1106 34-35 -0.303124253925553 1106 36-37 -0.16956420022552976 1106 38-39 -0.2917528059659773 1106 40-41 -0.23356136755575818 1106 42-43 -0.28004530034673536 1106 44-45 -0.1876513692306503 1106 46-47 -0.4759570705133953 1106 48-49 -0.32760728125954586 1106 50-51 -0.2397096642282719 1106 52-53 -0.19361048882610277 1106 54-55 -0.10414199051437834 1106 56-57 -0.22068944153952685 1106 58-59 -0.21723260859529603 1106 60-61 -0.2513639915363939 1106 62-63 -0.16539552288833903 1106 64-65 -0.1566623939371823 1106 66-67 -0.2787834653542447 1106 68-69 -0.3095763451235669 1106 70-71 -0.10312059288474273 1106 72-73 -0.2965474001439645 1106 74-75 -0.32808577084291457 1106 76-77 -0.18178851536628926 1106 78-79 -0.10165445126712669 1106 80-81 -0.035959846376652393 1106 82-83 -0.38311501845242546 1106 84-85 -0.36789837896803235 1106 86-87 -0.24599677239522322 1106 88-89 0.011978511125391833 1106 90-91 -0.060144355288059614 1106 92-93 -0.2924252489260972 1106 94-95 -0.36978471045150485 1106 96-97 -0.27471292362524125 1106 98-99 -0.10437500975894665 1106 100-101 0.3059376012122925 1107 1 0.028046866042096497 1107 2 -0.07839703685508681 1107 3 -0.021254436136956656 1107 4 -0.005194645715135948 1107 5 -0.06805669068465647 1107 6 -0.06793118756572625 1107 7 -0.19647727554145433 1107 8 -0.08491471997730571 1107 9 -0.12699109963429578 1107 10-11 0.05929563772849633 1107 12-13 -0.026679677068024432 1107 14-15 -0.01726588791029826 1107 16-17 0.019890939166010924 1107 18-19 0.08502483173766251 1107 20-21 0.0287178062845328 1107 22-23 -0.07609068140590836 1107 24-25 0.1401418197979254 1107 26-27 0.05658564135529076 1107 28-29 0.0877148736418718 1107 30-31 -0.002489331699010222 1107 32-33 -0.11608537981873468 1107 34-35 -0.08273740076791825 1107 36-37 -0.04838360453331347 1107 38-39 0.022195485175693364 1107 40-41 -0.2497714396090842 1107 42-43 -0.12527321234149014 1107 44-45 -0.014141563042535665 1107 46-47 -0.21155989099657546 1107 48-49 -0.20533239974295725 1107 50-51 -0.027163908438964768 1107 52-53 -0.3389963787039605 1107 54-55 -0.24860313180825244 1107 56-57 -0.36542415428949226 1107 58-59 -0.21561429857332826 1107 60-61 -0.09585210466397598 1107 62-63 -0.051146688456874756 1107 64-65 -0.1625268412072458 1107 66-67 -0.2167675134411624 1107 68-69 -7.180243128672714E-4 1107 70-71 -0.04173655250405517 1107 72-73 0.1661621132070863 1107 74-75 0.11926520977590016 1107 76-77 0.170899035205899 1107 78-79 -0.026410741484056643 1107 80-81 0.17386946044722507 1107 82-83 -0.15841267190289798 1107 84-85 -0.029189944490322972 1107 86-87 -0.21082935437032901 1107 88-89 0.03453467033627078 1107 90-91 0.07584377710511347 1107 92-93 0.13455429101148653 1107 94-95 0.401374693085657 1107 96-97 0.34868706866851795 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-0.9611384728358381 2316 90-91 -1.2195882195877346 2316 92-93 -0.965638626171927 2316 94-95 -0.7502168736774664 2316 96-97 -1.1580168034549487 2316 98-99 -0.6857068436411886 2316 100-101 -1.075625844312114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 4.0 5 11.0 6 25.0 7 60.0 8 148.0 9 285.0 10 545.0 11 828.0 12 1430.0 13 2261.0 14 2875.0 15 4448.0 16 6434.0 17 10256.0 18 9249.0 19 4772.0 20 3514.0 21 3384.0 22 3511.0 23 3653.0 24 4003.0 25 4646.0 26 5585.0 27 6766.0 28 8487.0 29 10770.0 30 13693.0 31 17661.0 32 23163.0 33 31008.0 34 44688.0 35 65877.0 36 105382.0 37 170998.0 38 152263.0 39 15531.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.713577328238166 20.4374990680405 15.51471953780231 20.33420406591902 2 15.586952399013846 21.554956517027442 40.53778006556311 22.3203110183956 3 19.500555389992144 26.66779550811411 30.56433041640703 23.267318685486714 4 12.570778358754842 17.910785402725473 38.33951938446533 31.178916854054346 5 12.527990268259545 38.96529816324236 34.15347810832522 14.353233460172879 6 29.814553927013627 37.939638590122186 17.734415214976565 14.51139226788762 7 27.290915987104114 34.63913196608057 20.80477364471296 17.265178402102354 8 26.484110427785758 35.07734821597898 19.44000433475116 18.99853702148411 9 26.81382243775568 17.174555010701418 20.75343393568313 35.25818861585977 10-11 24.469178163375748 28.060797717748876 27.06874726114287 20.4012768577325 12-13 26.04687519050412 25.48007039000343 28.0709264630378 20.402127956454652 14-15 22.825241797838043 26.38793313646339 27.530885102002113 23.25593996369646 16-17 20.7716534366449 29.96091950908943 27.987049930914903 21.280377123350764 18-19 20.607881119449488 27.633293598114385 31.761669962883694 19.997155319552437 20-21 21.780905421148166 26.48729376066755 31.866516756522444 19.865284061661836 22-23 21.35623458270174 26.67089308912197 31.765086770349477 20.207785557826806 24-25 21.076986118589712 26.738258033709577 32.089797383562434 20.09495846413828 26-27 20.961462655390882 26.83702261794942 32.167690075709245 20.033824650950464 28-29 20.5421434178701 27.091118322350532 32.303191752365045 20.063546507414323 30-31 20.797080541140232 26.752020741812387 32.16708050050188 20.283818216545495 32-33 20.781948470645606 26.9693316355558 31.899298312156265 20.34942158164233 34-35 21.17695266993579 26.949012489501772 31.816599387716398 20.057435452846033 36-37 21.237518109451187 26.603808216932766 31.89349509190695 20.265178581709097 38-39 20.828075736486877 27.08571660791027 31.999038223829697 20.087169431773155 40-41 21.02133648686907 27.05856934053445 31.785960534746465 20.134133637850017 42-43 20.832328547391125 27.220938203632407 31.391183714610083 20.55554953436639 44-45 21.07753859116428 27.119914876266478 31.177854283177293 20.62469224939195 46-47 21.35264555281623 27.605049985099296 30.497344964915605 20.544959497168865 48-49 21.493795887404836 27.80248435425754 30.111619842323428 20.5920999160142 50-51 21.3014413047601 28.14188182384655 29.91364362927041 20.643033242122943 52-53 21.529625314946763 28.44578851832787 29.364688033377583 20.65989813334778 54-55 21.309704424155402 28.264474005039148 29.437159654303596 20.988661916501854 56-57 21.448416461217523 28.3465633550974 29.376676329549454 20.82834385413562 58-59 21.682831676194088 28.000460567310558 29.479423478095963 20.837284278399395 60-61 21.39572214245076 28.113976863265695 30.03759042019994 20.452710574083607 62-63 21.327923925117172 28.053696729972096 29.940058519140635 20.678320825770097 64-65 21.194373760107936 28.19764311292238 29.747046779447032 20.860936347522653 66-67 21.253962233480532 28.26217116848636 29.299395844057326 21.184470753975777 68-69 21.255249112730624 28.235214434721357 29.51166318983501 20.997873262713014 70-71 21.369578174527916 28.42276015279998 29.24717563869849 20.960486033973613 72-73 21.435479938229797 28.114044593752542 29.289100810056624 21.16137465796104 74-75 21.261818969954756 28.70749911950367 28.950448375822923 21.080233534718648 76-77 21.324334209314298 28.203245644929698 29.207417842919455 21.265002302836553 78-79 21.3762157622389 28.239549185879547 28.91116469345182 21.473070358429737 80-81 21.35083129069335 28.497079798081735 28.840947712706043 21.311141198518868 82-83 21.390134195049825 28.592425121898522 28.91116630419396 21.10627437885769 84-85 21.123057312416268 28.434660811116512 28.872269264999318 21.570012611467902 86-87 20.963601636368562 28.502140283384357 28.79995123404947 21.734306846197608 88-89 21.282679959903554 28.389233561810844 28.689956923410364 21.638129554875242 90-91 21.043669098372312 28.15061673203146 28.868952063563025 21.936762106033203 92-93 21.130245427237107 28.017854179578094 28.97890483979667 21.872995553388126 94-95 20.915987104115306 28.052342120235156 29.05251821950096 21.979152556148573 96-97 21.038308363360517 27.96205738126846 29.126276719677062 21.873357535693966 98-99 20.852159624826523 28.165644724427796 28.598240222915035 22.38395542783065 100-101 20.478304191766032 28.547536779473536 28.583369629052108 22.390789399708325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 289.0 1 209.0 2 151.5 3 200.0 4 198.5 5 187.0 6 345.0 7 742.0 8 1257.5 9 1602.5 10 1575.0 11 1466.5 12 1490.0 13 1562.0 14 1681.0 15 1796.0 16 1865.5 17 1904.5 18 1981.0 19 2080.5 20 2222.5 21 2401.5 22 2654.0 23 3110.0 24 3630.5 25 4248.5 26 5209.0 27 6459.0 28 7432.5 29 8631.5 30 10352.0 31 12002.5 32 13722.5 33 15812.0 34 18115.0 35 19894.0 36 21996.5 37 24125.0 38 25191.0 39 25648.0 40 25568.0 41 25748.5 42 26128.0 43 26583.0 44 27496.0 45 28127.5 46 27691.5 47 27881.0 48 30293.5 49 29323.0 50 25548.5 51 23186.5 52 21123.0 53 19790.0 54 18501.5 55 16895.0 56 14831.5 57 12832.5 58 11464.0 59 10040.0 60 8315.0 61 6532.5 62 5099.0 63 3851.0 64 2759.0 65 1966.5 66 1388.0 67 1082.0 68 809.5 69 536.5 70 373.0 71 258.5 72 216.0 73 182.5 74 141.5 75 124.5 76 97.0 77 49.5 78 26.0 79 18.5 80 13.0 81 11.0 82 7.5 83 6.0 84 3.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07138793313646338 2 0.0 3 0.0 4 0.0 5 0.0023028365527891412 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.0319146054021835E-4 12-13 0.006366665763593508 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.482268158543524E-4 24-25 3.386524342336973E-4 26-27 1.354609736934789E-4 28-29 0.0027769499607163176 30-31 1.354609736934789E-4 32-33 0.0 34-35 0.0 36-37 6.773048684673945E-5 38-39 3.386524342336973E-4 40-41 1.354609736934789E-4 42-43 0.011649643737639187 44-45 6.773048684673945E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.773048684673945E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.773048684673945E-5 82-83 4.741134079271762E-4 84-85 4.063829210804367E-4 86-87 0.0 88-89 0.0 90-91 0.007450353553141341 92-93 0.002370567039635881 94-95 0.0 96-97 0.0 98-99 2.0319146054021835E-4 100-101 0.009549998645390263 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 738220.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.4477092900756 #Duplication Level Percentage of deduplicated Percentage of total 1 83.5259111182806 53.83053637939642 2 9.976586757264446 12.859363260787942 3 2.770088396379543 5.355775550330414 4 1.107154289226081 2.85414231085211 5 0.6534470419398986 2.105658249770121 6 0.39654437343890286 1.5333825900003353 7 0.2663630038796079 1.2016539807764974 8 0.20310507200415961 1.047172530869116 9 0.15823162319860787 0.9177899087160596 >10 0.8560682617761205 10.109670825219428 >50 0.05752599791383073 2.5346678659811146 >100 0.027282148512694265 3.1278159143770248 >500 6.344685695555247E-4 0.2595154914668876 >1k 8.459580927406995E-4 1.469179350063856 >5k 2.1148952318517488E-4 0.7936757913926926 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5823 0.7887892498171277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4818 0.6526509712551815 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2995 0.4057056162119693 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1607 0.2176857847254206 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1359 0.18409146324943784 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 797 0.1079623960337027 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 6.773048684673945E-5 6.773048684673945E-5 24-25 0.0 0.0 0.0 2.709219473869578E-4 1.354609736934789E-4 26-27 0.0 0.0 0.0 0.0011514182763945708 1.354609736934789E-4 28-29 6.773048684673945E-5 0.0 0.0 0.00372517677657067 1.354609736934789E-4 30-31 1.354609736934789E-4 0.0 0.0 0.009414537671696784 1.354609736934789E-4 32-33 1.354609736934789E-4 0.0 0.0 0.01605212538267725 1.354609736934789E-4 34-35 1.354609736934789E-4 0.0 0.0 0.024450705751672942 1.354609736934789E-4 36-37 1.354609736934789E-4 0.0 0.0 0.032713825146975156 1.354609736934789E-4 38-39 1.354609736934789E-4 0.0 0.0 0.04145105795020454 1.354609736934789E-4 40-41 1.354609736934789E-4 0.0 0.0 0.05289751022730352 1.354609736934789E-4 42-43 1.354609736934789E-4 0.0 0.0 0.06569857224133727 1.354609736934789E-4 44-45 1.354609736934789E-4 0.0 0.0 0.07667091111050905 1.354609736934789E-4 46-47 2.709219473869578E-4 0.0 0.0 0.09130069626940479 1.354609736934789E-4 48-49 2.709219473869578E-4 0.0 0.0 0.10897835333640378 1.354609736934789E-4 50-51 2.709219473869578E-4 0.0 0.0 0.13363225054861694 1.354609736934789E-4 52-53 2.709219473869578E-4 0.0 0.0 0.18903578878924981 1.354609736934789E-4 54-55 2.709219473869578E-4 0.0 0.0 0.26889003278155565 1.354609736934789E-4 56-57 2.709219473869578E-4 0.0 0.0 0.34671236216845924 1.354609736934789E-4 58-59 2.709219473869578E-4 0.0 0.0 0.4558261764785565 1.354609736934789E-4 60-61 4.063829210804367E-4 0.0 0.0 0.5944027525669855 1.354609736934789E-4 62-63 5.418438947739156E-4 0.0 0.0 0.6893608951261142 1.354609736934789E-4 64-65 5.418438947739156E-4 0.0 0.0 0.7612229416705047 1.354609736934789E-4 66-67 5.418438947739156E-4 0.0 0.0 0.9564222047628078 1.354609736934789E-4 68-69 5.418438947739156E-4 0.0 0.0 1.1521633117498848 1.354609736934789E-4 70-71 5.418438947739156E-4 0.0 0.0 1.261073934599442 1.354609736934789E-4 72-73 5.418438947739156E-4 0.0 0.0 1.4744249681666712 1.354609736934789E-4 74-75 5.418438947739156E-4 0.0 0.0 1.7733873371081792 1.354609736934789E-4 76-77 5.418438947739156E-4 0.0 0.0 2.0765489962341848 1.354609736934789E-4 78-79 5.418438947739156E-4 0.0 0.0 2.3623716507274253 1.354609736934789E-4 80-81 5.418438947739156E-4 0.0 0.0 2.679079407222779 1.354609736934789E-4 82-83 5.418438947739156E-4 0.0 0.0 2.9802768822302292 1.354609736934789E-4 84-85 5.418438947739156E-4 0.0 0.0 3.3210289615561757 1.354609736934789E-4 86-87 5.418438947739156E-4 0.0 0.0 3.900801928964265 1.354609736934789E-4 88-89 5.418438947739156E-4 0.0 0.0 4.555010701416922 1.354609736934789E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6725 0.0 28.269054 1 GGTATCA 4670 0.0 28.088966 1 TACCTGG 615 0.0 27.031593 2 TCAACGC 8270 0.0 22.801517 4 ATCAACG 8315 0.0 22.678116 3 CGTTTAA 105 0.0021881503 22.63207 1 CAACGCA 8365 0.0 22.600878 5 TATCAAC 8305 0.0 22.59104 2 AGTACTT 4685 0.0 22.356672 12-13 AACGCAG 8630 0.0 21.906876 6 GAGTACT 4560 0.0 21.823647 12-13 GTACATG 4770 0.0 21.621454 1 TACATGG 4800 0.0 21.473223 2 GTACCTG 775 0.0 20.850706 1 GTACTTT 4765 0.0 20.783653 14-15 ACATGGG 4710 0.0 20.270006 3 CGCAGAG 9515 0.0 19.820715 8 AGAGTAC 7680 0.0 19.762745 10-11 ACGCAGA 9545 0.0 19.758417 7 CATGGGG 1550 0.0 19.612232 4 >>END_MODULE