FastQCFastQC Report
Thu 26 May 2016
SRR937283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748100
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66190.37863966592300213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48140.2753847033922544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33560.19197986385218235No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28160.16108918254104457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77250.038.389661
GGTATCA45550.036.2050551
ATCAACG96850.030.3183043
TCAACGC98050.029.8979324
TATCAAC99450.029.716772
CAACGCA99650.029.4170445
AACGCAG102100.028.7510856
ACGCAGA114050.025.4068587
CGCAGAG114550.025.1300858
GTACATG74050.025.0303941
TACATGG76350.024.0211772
ACATGGG74850.023.8677833
ACACCGT2602.3646862E-1123.7498846
GCAGAGT120650.023.2689389
TAGACCG1452.331191E-522.9361825
AGAGTAC103700.021.55217210-11
GTGTAGC10650.021.4200151
AGTACTT65150.021.1440212-13
GAGTACT61700.021.09450712-13
GTACTTT65800.019.9230614-15
Produced by FastQC (version 0.11.5)