##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937283_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1748100 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.590071506206737 31.0 31.0 34.0 30.0 34.0 2 31.709084720553744 33.0 31.0 34.0 30.0 34.0 3 31.745818889079573 33.0 31.0 34.0 30.0 34.0 4 35.38012127452663 37.0 35.0 37.0 33.0 37.0 5 35.388747211257936 37.0 35.0 37.0 33.0 37.0 6 35.42224872718952 37.0 35.0 37.0 33.0 37.0 7 35.350196785080946 37.0 35.0 37.0 33.0 37.0 8 35.33525885246839 37.0 35.0 37.0 33.0 37.0 9 36.911340312339114 39.0 37.0 39.0 33.0 39.0 10-11 36.79127881700131 39.0 37.0 39.0 33.0 39.0 12-13 36.631817115725646 39.0 37.0 39.0 32.0 39.0 14-15 37.69070133287569 40.0 37.0 41.0 32.0 41.0 16-17 37.60519135060923 40.0 37.0 41.0 32.0 41.0 18-19 37.50653595332075 40.0 37.0 41.0 32.0 41.0 20-21 37.36347148332475 40.0 37.0 41.0 32.0 41.0 22-23 37.44870459355872 40.0 37.0 41.0 32.0 41.0 24-25 37.412459813511816 40.0 37.0 41.0 32.0 41.0 26-27 37.32300268863337 40.0 37.0 41.0 32.0 41.0 28-29 37.171144957382296 39.5 37.0 41.0 31.0 41.0 30-31 36.91126451575997 39.0 36.0 41.0 30.5 41.0 32-33 36.658176305703336 39.0 36.0 40.5 30.0 41.0 34-35 36.62436616898347 39.0 36.0 41.0 30.0 41.0 36-37 36.46278130541731 39.0 35.5 41.0 30.0 41.0 38-39 36.299173960299754 39.0 35.0 40.5 30.0 41.0 40-41 36.04267376008238 39.0 35.0 40.0 29.5 41.0 42-43 35.75447400034323 38.5 35.0 40.0 28.0 41.0 44-45 35.48978605342944 38.0 35.0 40.0 27.0 41.0 46-47 35.078382243578744 38.0 34.5 40.0 25.5 41.0 48-49 34.71596876608889 38.0 34.0 40.0 24.0 41.0 50-51 33.75649562382015 37.5 33.0 39.5 23.5 40.5 52-53 33.8636365196499 37.5 33.0 39.5 23.0 40.0 54-55 34.1328173445455 38.0 33.5 40.0 23.0 41.0 56-57 33.82535896115783 38.0 33.0 40.0 22.0 41.0 58-59 33.50461243635948 37.5 33.0 40.0 20.5 41.0 60-61 33.12888993764659 37.0 32.0 40.0 18.5 41.0 62-63 32.69114009496025 36.5 31.5 39.0 17.0 41.0 64-65 32.21154968251244 36.0 31.0 39.0 13.0 40.5 66-67 31.72425890967336 35.5 31.0 39.0 8.0 40.0 68-69 31.184794920199074 35.0 30.0 38.0 7.5 40.0 70-71 30.655092672043935 35.0 29.5 38.0 3.5 40.0 72-73 30.102564784623304 34.5 29.0 37.0 2.0 39.0 74-75 29.48817315943024 34.0 28.5 36.5 2.0 39.0 76-77 28.89543103941422 34.0 27.5 36.0 2.0 39.0 78-79 28.276376351467306 34.0 26.5 35.5 2.0 37.5 80-81 27.611521080029746 33.0 26.0 35.0 2.0 37.0 82-83 26.969578971454723 32.5 25.0 35.0 2.0 36.5 84-85 26.28231165265145 32.0 23.5 35.0 2.0 36.0 86-87 25.57245552313941 31.5 20.5 34.5 2.0 35.5 88-89 24.897225845203366 31.0 19.0 34.0 2.0 35.0 90-91 24.212558778101936 31.0 13.5 34.0 2.0 35.0 92-93 23.410787998398263 30.5 4.5 34.0 2.0 35.0 94-95 22.51755877810194 30.0 2.0 34.0 2.0 35.0 96-97 21.53138064183971 29.0 2.0 34.0 2.0 35.0 98-99 20.22247325667868 29.0 2.0 34.0 2.0 35.0 100-101 17.227016475030034 22.5 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3740046380466673 1101 2 0.3586644954684992 1101 3 0.3158051955958392 1101 4 0.26936764607903996 1101 5 0.23680797944270182 1101 6 0.27378003336651346 1101 7 0.3402052766141779 1101 8 0.24404606021867892 1101 9 0.35901606702745426 1101 10-11 0.43190241458238887 1101 12-13 0.4905157687916315 1101 14-15 0.6301182404897858 1101 16-17 0.6247595500992631 1101 18-19 0.6557549444487947 1101 20-21 0.6981549069690018 1101 22-23 0.6472507059254653 1101 24-25 0.636739985339247 1101 26-27 0.6685180547363458 1101 28-29 0.6465918431871458 1101 30-31 0.5886135588050223 1101 32-33 0.4894290811808375 1101 34-35 0.7768371192987686 1101 36-37 0.7722485480319747 1101 38-39 0.6760874534395853 1101 40-41 0.8026502011906942 1101 42-43 0.9391875804246013 1101 44-45 0.9913219804998761 1101 46-47 1.0346683123793454 1101 48-49 1.0748867359548697 1101 50-51 1.0757299042464723 1101 52-53 1.1252113242320334 1101 54-55 1.2369067740095758 1101 56-57 1.2726355169552548 1101 58-59 1.2570728655804047 1101 60-61 1.3387371048204528 1101 62-63 1.3086508631909197 1101 64-65 1.2020873937938177 1101 66-67 1.390897182470443 1101 68-69 1.5129768197494151 1101 70-71 1.5057614465034952 1101 72-73 1.6065917193695434 1101 74-75 1.5974530071900013 1101 76-77 1.6006497563215056 1101 78-79 1.8368424671624126 1101 80-81 1.8864825861506986 1101 82-83 1.8902385846165153 1101 84-85 2.031698048885623 1101 86-87 2.1642654066121594 1101 88-89 2.1272700434578056 1101 90-91 2.059723037789375 1101 92-93 2.1948202193035407 1101 94-95 2.2146879532406096 1101 96-97 2.5371574812673323 1101 98-99 2.7364494803722046 1101 100-101 2.564679222155803 1102 1 0.3752239829274764 1102 2 0.326119477461301 1102 3 0.13145431238541505 1102 4 0.20554840409653252 1102 5 0.23981603980970334 1102 6 0.22795770443493524 1102 7 0.20083295625746445 1102 8 0.1601719391416765 1102 9 0.32067615963593 1102 10-11 0.210202020138901 1102 12-13 0.2522650399286519 1102 14-15 0.46573477281700804 1102 16-17 0.4119084096430825 1102 18-19 0.4581198904271915 1102 20-21 0.4990827066603103 1102 22-23 0.49085300398754583 1102 24-25 0.5298869584141954 1102 26-27 0.5970508964444505 1102 28-29 0.686414343615894 1102 30-31 0.6105849187775831 1102 32-33 0.5348646839933906 1102 34-35 0.6416364676095228 1102 36-37 0.9029371091707148 1102 38-39 0.7438545602823226 1102 40-41 0.8247939158193986 1102 42-43 0.9592510343776084 1102 44-45 0.8999019839298157 1102 46-47 0.8238562732779897 1102 48-49 0.8519678541164168 1102 50-51 0.6533855379285143 1102 52-53 0.7346711081455979 1102 54-55 1.064132807280025 1102 56-57 0.994873555027624 1102 58-59 1.0526370912706895 1102 60-61 0.9195542888369204 1102 62-63 0.8594903418395248 1102 64-65 0.7816415011510571 1102 66-67 1.1017152239727572 1102 68-69 1.1340203800306732 1102 70-71 1.307370947446735 1102 72-73 1.3930811722935665 1102 74-75 1.5606917312510582 1102 76-77 1.7752175834523563 1102 78-79 1.6325164510416776 1102 80-81 1.7967621265382832 1102 82-83 1.8837336969471572 1102 84-85 1.926709196201692 1102 86-87 2.0731669672364106 1102 88-89 2.111487158875402 1102 90-91 1.8220862687960668 1102 92-93 1.8542105468631327 1102 94-95 2.0450009355764003 1102 96-97 1.7002487177619336 1102 98-99 2.0382553455754255 1102 100-101 2.195542710408244 1103 1 0.4346189007991619 1103 2 0.2833369420127525 1103 3 0.3710373532389859 1103 4 0.30977274777195163 1103 5 0.3750511935370682 1103 6 0.377756946327402 1103 7 0.23947397829874006 1103 8 0.2976715881659615 1103 9 0.37270199144836624 1103 10-11 0.361248629713522 1103 12-13 0.2959046580670801 1103 14-15 0.4262549188228988 1103 16-17 0.4918227715692396 1103 18-19 0.40918755854455213 1103 20-21 0.5791220159401078 1103 22-23 0.4664166709817934 1103 24-25 0.37743784989250173 1103 26-27 0.3166177924152507 1103 28-29 0.18293757820806178 1103 30-31 0.21457621457991394 1103 32-33 0.12518407189174496 1103 34-35 0.30828886126388255 1103 36-37 0.34279952669142233 1103 38-39 0.30492433425396115 1103 40-41 0.5685065369060212 1103 42-43 0.4493191236658376 1103 44-45 0.4764792687817092 1103 46-47 0.24792057202851936 1103 48-49 0.42166390914940877 1103 50-51 0.5148336637182069 1103 52-53 0.4829751935028881 1103 54-55 0.7508918975509786 1103 56-57 0.8004351161675984 1103 58-59 0.7048615753363876 1103 60-61 0.9969184612361843 1103 62-63 0.9679650013930754 1103 64-65 0.7688708988767274 1103 66-67 1.0860736403703406 1103 68-69 1.1981871636892443 1103 70-71 1.400161204360959 1103 72-73 1.5711402766035505 1103 74-75 1.3540849644393198 1103 76-77 1.228686627394513 1103 78-79 1.1960184867008117 1103 80-81 1.1804178895594823 1103 82-83 1.535434525249844 1103 84-85 1.6172883061272856 1103 86-87 1.8837432693563017 1103 88-89 1.7333796199170983 1103 90-91 1.8663134202771836 1103 92-93 1.9133197705048595 1103 94-95 1.8919453410235398 1103 96-97 1.7745460643855466 1103 98-99 2.1026678122327347 1103 100-101 2.1180900921294317 1104 1 0.1683703577564799 1104 2 0.2533014200057586 1104 3 0.09533940130470242 1104 4 0.21112979944536647 1104 5 0.1699254789341893 1104 6 0.18693855258815262 1104 7 0.23185894804952767 1104 8 0.32846195282157 1104 9 0.3146706876797012 1104 10-11 0.2321719243796636 1104 12-13 0.3890673655100514 1104 14-15 0.5286554294517529 1104 16-17 0.4610670963735899 1104 18-19 0.5908285087582428 1104 20-21 0.41926396521024145 1104 22-23 0.4525561503623763 1104 24-25 0.49975357948122223 1104 26-27 0.4052417309177798 1104 28-29 0.5376386938210871 1104 30-31 0.4182935371086316 1104 32-33 0.3949057236650688 1104 34-35 0.4524846208242934 1104 36-37 0.5472154949436288 1104 38-39 0.6965905383944957 1104 40-41 0.7331454890068159 1104 42-43 0.5948837293160523 1104 44-45 0.5272549251062912 1104 46-47 0.7094513485173977 1104 48-49 0.744259235785357 1104 50-51 0.5211728156302939 1104 52-53 0.49224976751632 1104 54-55 0.8098168018080756 1104 56-57 0.757653992784121 1104 58-59 0.7476815509150114 1104 60-61 0.8270337690010834 1104 62-63 0.8802735155741317 1104 64-65 0.9881839088117914 1104 66-67 1.0391741843010784 1104 68-69 1.0270709956257456 1104 70-71 0.8676342853400314 1104 72-73 1.0863335662744156 1104 74-75 1.0574324973249283 1104 76-77 1.1225944644373094 1104 78-79 1.1113297205355224 1104 80-81 1.2057703344726178 1104 82-83 1.3802692646626191 1104 84-85 1.5419656941497095 1104 86-87 1.4309063653212242 1104 88-89 1.5815008387659617 1104 90-91 1.7771615081941512 1104 92-93 1.7967652664253784 1104 94-95 1.754604218750135 1104 96-97 1.8549375002516726 1104 98-99 1.9820754550145523 1104 100-101 1.9023792357160438 1105 1 0.19629469955416212 1105 2 0.1437493048086651 1105 3 0.1394432977555553 1105 4 0.18867878893283319 1105 5 0.19327145758852993 1105 6 0.1729700367659177 1105 7 0.1555702607140006 1105 8 0.23107405759962774 1105 9 0.2747971854155651 1105 10-11 0.24184526100790293 1105 12-13 0.3201800313129155 1105 14-15 0.3676924036579692 1105 16-17 0.33615225192439624 1105 18-19 0.39613286471512055 1105 20-21 0.39516778285045717 1105 22-23 0.4548623630442066 1105 24-25 0.37131413008699354 1105 26-27 0.42259993857710043 1105 28-29 0.4456860603439381 1105 30-31 0.3920355725600544 1105 32-33 0.5046405058672292 1105 34-35 0.5825184826786156 1105 36-37 0.6206140696734721 1105 38-39 0.5849786606979066 1105 40-41 0.4651716568066604 1105 42-43 0.5433497605998667 1105 44-45 0.49797577519245095 1105 46-47 0.6949552022234968 1105 48-49 0.7284580389324375 1105 50-51 0.6435308229236156 1105 52-53 0.660301408513881 1105 54-55 0.8898115922920269 1105 56-57 1.1087097546424545 1105 58-59 0.8805674558346581 1105 60-61 0.9980853899620357 1105 62-63 0.9700483375823339 1105 64-65 1.083217336539775 1105 66-67 1.0983006887157991 1105 68-69 0.9056007766924985 1105 70-71 1.1258020384356868 1105 72-73 1.2996480540721258 1105 74-75 1.021494684253053 1105 76-77 1.1306747243788031 1105 78-79 1.2285793966104848 1105 80-81 1.3051555447901322 1105 82-83 1.4499366904938036 1105 84-85 1.521091397100431 1105 86-87 1.5143152255058254 1105 88-89 1.6746222739208427 1105 90-91 1.6428762137229143 1105 92-93 1.8712685257123418 1105 94-95 2.0033789806485345 1105 96-97 2.0221801635165697 1105 98-99 1.9001362794538643 1105 100-101 1.5583546508076118 1106 1 -0.004048327972476784 1106 2 -0.65078895470878 1106 3 -0.05446871458249092 1106 4 0.05100453425341556 1106 5 0.014468943388337152 1106 6 0.04598176705243162 1106 7 0.08045697785913575 1106 8 0.0852049227011662 1106 9 0.026924920616202996 1106 10-11 -0.23238795832778436 1106 12-13 -0.12429509952514906 1106 14-15 -0.054843550319340295 1106 16-17 -0.11885533920334979 1106 18-19 0.19741202564269855 1106 20-21 0.017602377166511474 1106 22-23 0.10689651494218566 1106 24-25 0.1567051557686696 1106 26-27 -0.04839207446848803 1106 28-29 0.16711520153024395 1106 30-31 0.4140403311575227 1106 32-33 0.40709543219040256 1106 34-35 0.4204957149691282 1106 36-37 0.3723251188777894 1106 38-39 0.42699876508800827 1106 40-41 0.6472999827435544 1106 42-43 0.3515390338307398 1106 44-45 0.3055960058732836 1106 46-47 0.6177898618209028 1106 48-49 0.6850464336016628 1106 50-51 0.5306364522396194 1106 52-53 0.5243207646380625 1106 54-55 0.7901236812589119 1106 56-57 0.35873553677558334 1106 58-59 0.32250942951452544 1106 60-61 0.7073857479749037 1106 62-63 0.7161053399596327 1106 64-65 0.8294570666567438 1106 66-67 0.9051003967519229 1106 68-69 0.9061147506086549 1106 70-71 0.8589314286990835 1106 72-73 0.8192513196087425 1106 74-75 0.7509089442257313 1106 76-77 0.8521278955144282 1106 78-79 1.1032815670822842 1106 80-81 1.085907288379893 1106 82-83 0.895481654700859 1106 84-85 0.8608046263551579 1106 86-87 1.0945076720250064 1106 88-89 1.4845230135426597 1106 90-91 1.3597189720807492 1106 92-93 1.4672284571068381 1106 94-95 1.2475102495867212 1106 96-97 1.1688239305315271 1106 98-99 1.154562212308175 1106 100-101 0.9180277567219477 1107 1 0.12498632536445342 1107 2 0.2512665362848274 1107 3 0.1366210665870966 1107 4 0.09536983266796284 1107 5 0.044964411658440895 1107 6 0.09975260989712353 1107 7 0.19920217894654257 1107 8 0.06425150846941108 1107 9 0.2007457499720644 1107 10-11 0.25391065984478445 1107 12-13 0.19723155778579127 1107 14-15 0.1302036541195264 1107 16-17 0.2516090398951789 1107 18-19 0.2543199954692099 1107 20-21 0.1766910312401393 1107 22-23 0.1909061815085451 1107 24-25 0.194331548750327 1107 26-27 0.247221589721768 1107 28-29 0.3268089076915217 1107 30-31 0.35131627381959873 1107 32-33 0.2832713279009553 1107 34-35 0.3482383583658262 1107 36-37 0.3491037320698709 1107 38-39 0.49309957078652644 1107 40-41 0.4960984063656184 1107 42-43 0.4077587812263559 1107 44-45 0.5101232286777204 1107 46-47 0.616282743866229 1107 48-49 0.6239039097886803 1107 50-51 0.38565838456717216 1107 52-53 0.42667018902795206 1107 54-55 0.5624875866917947 1107 56-57 0.7521943270864497 1107 58-59 0.740467317111019 1107 60-61 0.7007360115259971 1107 62-63 0.6582272115874233 1107 64-65 0.5572907921174419 1107 66-67 0.8272036693509115 1107 68-69 0.8231478747785737 1107 70-71 0.8757880955559791 1107 72-73 0.9687580367893638 1107 74-75 0.9600851136039985 1107 76-77 0.8706114910153815 1107 78-79 0.8240736989408362 1107 80-81 1.094459080319794 1107 82-83 1.0267523419051408 1107 84-85 0.9465247161596722 1107 86-87 0.7856302025305268 1107 88-89 0.7155877366064942 1107 90-91 0.8864586427456445 1107 92-93 1.0604008042160764 1107 94-95 1.2105038242318606 1107 96-97 1.3790978511652945 1107 98-99 1.211528060911565 1107 100-101 1.3460396904502652 1108 1 0.13748819587522831 1108 2 0.1590179329328727 1108 3 0.11523676391351145 1108 4 0.031637789571391295 1108 5 -0.022727502746981543 1108 6 0.04500338304157481 1108 7 0.1154369654589118 1108 8 0.10266618823796136 1108 9 0.19247372782521666 1108 10-11 0.18383166193975597 1108 12-13 0.20958347568284097 1108 14-15 0.3464643160309393 1108 16-17 0.2382984852263874 1108 18-19 0.04787670935588295 1108 20-21 0.13285529949632036 1108 22-23 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0.5648742938834381 2316 68-69 0.7324466196364625 2316 70-71 0.5719551790451334 2316 72-73 0.7205095677355153 2316 74-75 0.7997038963265126 2316 76-77 1.1574827512996002 2316 78-79 1.176360768873522 2316 80-81 1.1508528256451527 2316 82-83 1.3035935675969874 2316 84-85 1.537328229155186 2316 86-87 1.7102866161430939 2316 88-89 1.4634227917673002 2316 90-91 1.47864848006801 2316 92-93 1.7967698323574375 2316 94-95 1.9356084194076288 2316 96-97 1.9634971008848154 2316 98-99 2.0690229772354236 2316 100-101 2.719936766459984 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7443.0 3 1452.0 4 1409.0 5 1755.0 6 2030.0 7 2481.0 8 3001.0 9 3788.0 10 4831.0 11 6016.0 12 7598.0 13 9982.0 14 11144.0 15 13445.0 16 16597.0 17 20583.0 18 18320.0 19 15088.0 20 15981.0 21 17606.0 22 19382.0 23 21387.0 24 24508.0 25 27905.0 26 32224.0 27 37029.0 28 43650.0 29 51068.0 30 59586.0 31 72310.0 32 88281.0 33 110081.0 34 139710.0 35 180714.0 36 231355.0 37 262137.0 38 153898.0 39 12324.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84080764314254 19.430872292231324 15.195634063666844 22.532686000959295 2 14.796113832142325 20.183526227044883 41.15799314070727 23.862366800105526 3 18.895817478282762 24.974120542926638 29.683235445422017 26.446826533368583 4 12.281005613505446 16.710272462624424 38.00592090623588 33.002801017634255 5 13.481861784463412 37.74949975310256 34.24097533950548 14.52766312292854 6 31.29655493482309 36.672560567282204 17.782953096476504 14.247931401418207 7 28.216412827351807 32.92839827812777 20.701511568972357 18.153677325548063 8 26.039939454401594 35.33265678213905 19.774387377160092 18.853016386299267 9 26.245525991965422 17.05151832436716 20.43301402209452 36.2699416615729 10-11 24.55218467131728 27.183194703688095 27.495950064655606 20.768670560339018 12-13 25.5663493514309 24.376754095362006 28.144484274102332 21.91241227910476 14-15 22.42811201184311 25.710107634893358 26.560098203294807 25.301682149968723 16-17 21.718979418319122 29.312197250233773 26.948333955387533 22.020489376059572 18-19 21.620523133296494 27.675428740458102 29.531899574183164 21.172148552062243 20-21 22.429917257676436 26.707287779670256 29.666158544450568 21.19663641820274 22-23 21.979255464602264 26.717976705536632 29.568845475397037 21.733922354464067 24-25 21.851946762428256 26.916378304130177 29.66493763801845 21.56673729542312 26-27 21.707926269288848 27.16498261295275 29.48600723915703 21.64108387860138 28-29 21.491350408435018 27.24372655565796 29.64121646124275 21.623706574664272 30-31 21.651496194289084 26.96816666456417 29.608310521239616 21.77202661990713 32-33 21.497336566741215 27.079179408554694 29.535311812765848 21.888172211938247 34-35 22.010504591350983 27.134854218048392 29.284078356992698 21.570562833607926 36-37 21.940723987737183 26.955579538467493 29.28003967213162 21.823656801663706 38-39 21.68096875895672 27.217827457724276 29.36543422184007 21.735769561478936 40-41 21.978144494651627 27.030825732037332 29.32350997306467 21.667519800246367 42-43 21.812757997288923 27.08760492236111 29.0842219528408 22.015415127509172 44-45 21.85356963813331 27.25810894438185 28.894931768235864 21.993389649248975 46-47 21.921996643746986 27.45580083097935 28.559482905412032 22.062719619861635 48-49 22.09121520704485 27.557235977178422 28.302818754697878 22.048730061078846 50-51 22.06389327877388 27.60245953341575 28.293350337288437 22.04029685052193 52-53 22.205992454591776 27.68373975137487 28.12894005566868 21.981327738364676 54-55 21.961291676437586 27.577731132186358 28.212406134666118 22.24857105670994 56-57 22.03800026826956 27.949274124483953 28.0326784422481 21.98004716499839 58-59 22.205272480864195 27.59883429849421 28.105712126522576 22.090181094119018 60-61 22.1390090499579 27.479768136742695 28.527342749171897 21.853880064127512 62-63 22.015317425879125 27.634305485257222 28.434827568615155 21.9155495202485 64-65 21.947519088618243 27.63743618857546 28.28270785981361 22.132336862992684 66-67 21.88391222269383 27.904508690863537 28.032760151998758 22.17881893444387 68-69 21.84463459483751 27.73901973192463 28.286141567273344 22.130204105964516 70-71 21.854995139220073 27.66703772148648 28.207197622803477 22.270769516489967 72-73 22.03513315443174 27.5782737458044 28.11414735564129 22.272445744122574 74-75 21.933902304594426 27.837564313079987 27.984970995879323 22.243562386446268 76-77 21.98491986960824 27.64246262932255 28.03233651439389 22.34028098667532 78-79 22.000624899672072 27.665025110647374 28.016287522645445 22.31806246703511 80-81 21.87400486521486 27.941335380235717 27.942969156876668 22.241690597672754 82-83 21.98081970112072 27.877868826382496 27.92547421241174 22.215837260085046 84-85 22.056430223930963 27.763673612908164 27.779234523556724 22.40066163960415 86-87 22.048535286666553 27.65293372537556 27.94504530838936 22.353485679568525 88-89 22.144280363695977 27.53291136773488 27.797308622245254 22.52549964632389 90-91 22.050815838656803 27.545141180742704 28.004226867088416 22.399816113512074 92-93 22.219693617138365 27.36408015206789 28.035768743078183 22.380457487715567 94-95 22.019527655102188 27.362218885484317 27.954720740545635 22.663532718867856 96-97 22.079157179316088 27.303505791010004 27.957072461574796 22.660264568099116 98-99 21.893070666895213 27.332035059136068 27.754881856856954 23.020012417111765 100-101 22.397886790116843 26.51402620992414 28.473250567665794 22.614836432293224 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2594.0 1 1455.5 2 304.5 3 302.5 4 289.0 5 285.0 6 460.0 7 928.5 8 1560.5 9 1912.0 10 1822.0 11 1699.0 12 1755.5 13 1931.0 14 2061.5 15 2219.0 16 2365.0 17 2465.5 18 2663.5 19 2909.5 20 3223.0 21 3696.0 22 4437.5 23 5420.5 24 6655.5 25 8257.0 26 10466.0 27 13007.5 28 15545.0 29 18580.0 30 22139.0 31 25861.0 32 29495.5 33 33249.5 34 37548.0 35 40925.5 36 44381.5 37 48155.5 38 50373.0 39 52213.0 40 54416.5 41 57310.0 42 60064.0 43 63137.5 44 65914.5 45 66981.0 46 66509.5 47 66556.0 48 69385.5 49 69077.0 50 64928.5 51 61615.5 52 58482.5 53 56240.0 54 53873.0 55 49620.0 56 44762.0 57 39436.5 58 34652.5 59 30381.0 60 25646.5 61 20688.5 62 16042.5 63 12174.5 64 9185.0 65 7156.5 66 5344.5 67 3968.0 68 2919.5 69 1951.5 70 1423.0 71 1056.5 72 756.0 73 601.5 74 476.0 75 357.0 76 253.5 77 156.0 78 87.5 79 55.5 80 41.5 81 24.0 82 16.5 83 11.5 84 10.0 85 7.5 86 4.5 87 3.0 88 3.0 89 6.0 90 5.5 91 2.5 92 2.0 93 1.0 94 1.5 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29471998169441105 2 0.2562782449516618 3 0.253932841370631 4 0.25444768605914997 5 0.25519135060923287 6 0.22447228419426807 7 0.22607402322521597 8 0.22653166294834393 9 0.2367141467879412 10-11 0.24283507808477775 12-13 0.23714318402837364 14-15 0.2267032778445169 16-17 0.22189806075167323 18-19 0.21097191236199303 20-21 0.2156055145586637 22-23 0.21377495566615182 24-25 0.21554830959327267 26-27 0.21154396201590298 28-29 0.2255305760540015 30-31 0.23694296664950515 32-33 0.2297637434929352 34-35 0.22913448887363422 36-37 0.21752188089926208 38-39 0.20593787540758535 40-41 0.20313483210342656 42-43 0.21592014186831418 44-45 0.21248784394485443 46-47 0.2226989302671472 48-49 0.2265888679137349 50-51 0.23980321491905496 52-53 0.25115840054916766 54-55 0.22761855729077282 56-57 0.20513700589211142 58-59 0.19446827984669068 60-61 0.197414335564327 62-63 0.20313483210342656 64-65 0.19498312453520963 66-67 0.19910188204336135 68-69 0.2011326583147417 70-71 0.2015902980378697 72-73 0.203964304101596 74-75 0.20630970768262688 76-77 0.21840855786282248 78-79 0.2185229677936045 80-81 0.21025685029460559 82-83 0.20304902465534008 84-85 0.1912648017847949 86-87 0.19392483267547622 88-89 0.2039929065842915 90-91 0.2028202047937761 92-93 0.20685315485384131 94-95 0.2029918196899491 96-97 0.2100566329157371 98-99 0.21354613580458784 100-101 0.19767175790858646 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1748100.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.61759364757842 #Duplication Level Percentage of deduplicated Percentage of total 1 78.37286300263074 46.72401499488173 2 12.5520565637395 14.966468153168822 3 4.030527311687044 7.208710183608746 4 1.7230790732714472 4.109033120517725 5 0.8939130749123934 2.664647322819219 6 0.5408437904038529 1.9346283193867786 7 0.356156104031851 1.4863218919692889 8 0.24706017933489657 1.1783306702468572 9 0.1842101196157722 0.9883947651322429 >10 0.9933582870635185 10.827401613418333 >50 0.06952886847134675 2.830955062602896 >100 0.035155181724707815 3.709936753961757 >500 7.682726848303586E-4 0.29216313531678917 >1k 3.841363424151793E-4 0.7000350098997415 >5k 9.603408560379483E-5 0.3789590030691412 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6619 0.37863966592300213 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4814 0.2753847033922544 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3356 0.19197986385218235 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2816 0.16108918254104457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.720496539099594E-5 2 0.0 0.0 0.0 0.0 5.720496539099594E-5 3 0.0 0.0 0.0 0.0 5.720496539099594E-5 4 0.0 0.0 0.0 0.0 5.720496539099594E-5 5 0.0 0.0 0.0 0.0 5.720496539099594E-5 6 0.0 0.0 0.0 0.0 5.720496539099594E-5 7 0.0 0.0 0.0 0.0 5.720496539099594E-5 8 0.0 0.0 0.0 0.0 5.720496539099594E-5 9 0.0 0.0 0.0 0.0 5.720496539099594E-5 10-11 0.0 0.0 0.0 2.860248269549797E-5 5.720496539099594E-5 12-13 0.0 0.0 0.0 5.720496539099594E-5 5.720496539099594E-5 14-15 0.0 0.0 0.0 5.720496539099594E-5 5.720496539099594E-5 16-17 0.0 0.0 0.0 5.720496539099594E-5 5.720496539099594E-5 18-19 2.860248269549797E-5 0.0 0.0 5.720496539099594E-5 5.720496539099594E-5 20-21 5.720496539099594E-5 0.0 0.0 1.4301241347748986E-4 5.720496539099594E-5 22-23 5.720496539099594E-5 0.0 0.0 2.860248269549797E-4 8.580744808649391E-5 24-25 5.720496539099594E-5 0.0 0.0 4.0043475773697155E-4 1.1440993078199188E-4 26-27 5.720496539099594E-5 0.0 0.0 8.008695154739431E-4 1.1440993078199188E-4 28-29 5.720496539099594E-5 0.0 0.0 0.0011727017905154168 1.1440993078199188E-4 30-31 5.720496539099594E-5 0.0 0.0 0.0026028259252903153 1.4301241347748986E-4 32-33 5.720496539099594E-5 0.0 0.0 0.004633602196670671 1.7161489617298782E-4 34-35 8.580744808649391E-5 0.0 0.0 0.006635775985355528 1.7161489617298782E-4 36-37 1.1440993078199188E-4 0.0 0.0 0.009810651564555803 1.7161489617298782E-4 38-39 1.1440993078199188E-4 0.0 0.0 0.01618900520565185 1.7161489617298782E-4 40-41 1.1440993078199188E-4 0.0 0.0 0.022710371260225386 1.7161489617298782E-4 42-43 1.7161489617298782E-4 0.0 0.0 0.02785881814541502 1.7161489617298782E-4 44-45 1.7161489617298782E-4 0.0 0.0 0.032778445169040674 1.7161489617298782E-4 46-47 1.7161489617298782E-4 0.0 0.0 0.039214003775527716 1.7161489617298782E-4 48-49 1.7161489617298782E-4 0.0 0.0 0.046507636862879696 1.7161489617298782E-4 50-51 1.7161489617298782E-4 0.0 0.0 0.05374406498484068 1.7161489617298782E-4 52-53 1.7161489617298782E-4 0.0 0.0 0.06821692122876266 1.7161489617298782E-4 54-55 1.7161489617298782E-4 0.0 0.0 0.09066987014472856 2.0021737886848578E-4 56-57 1.7161489617298782E-4 0.0 0.0 0.12502145186202163 2.2881986156398376E-4 58-59 1.7161489617298782E-4 0.0 0.0 0.17107144900177335 2.2881986156398376E-4 60-61 1.7161489617298782E-4 0.0 0.0 0.22444368171157258 2.2881986156398376E-4 62-63 1.7161489617298782E-4 0.0 0.0 0.2684915050626394 2.2881986156398376E-4 64-65 1.7161489617298782E-4 0.0 0.0 0.32329386190721354 2.2881986156398376E-4 66-67 1.7161489617298782E-4 0.0 0.0 0.4084720553744065 2.2881986156398376E-4 68-69 1.7161489617298782E-4 0.0 0.0 0.5158743778960013 2.2881986156398376E-4 70-71 1.7161489617298782E-4 0.0 0.0 0.6332303643956296 2.2881986156398376E-4 72-73 2.0021737886848578E-4 0.0 0.0 0.7592815056346891 3.4322979234597563E-4 74-75 2.2881986156398376E-4 0.0 0.0 0.8910817458955438 3.4322979234597563E-4 76-77 2.2881986156398376E-4 0.0 0.0 1.0316057433785253 3.4322979234597563E-4 78-79 2.2881986156398376E-4 0.0 0.0 1.1765059207139181 3.4322979234597563E-4 80-81 2.860248269549797E-4 0.0 0.0 1.3224929923917395 4.0043475773697155E-4 82-83 2.860248269549797E-4 0.0 0.0 1.4971969566958412 4.576397231279675E-4 84-85 2.860248269549797E-4 0.0 0.0 1.7276185572907727 4.576397231279675E-4 86-87 2.860248269549797E-4 5.720496539099594E-5 0.0 1.9981694411074882 4.576397231279675E-4 88-89 2.860248269549797E-4 5.720496539099594E-5 0.0 2.31848864481437 4.576397231279675E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7725 0.0 38.38966 1 GGTATCA 4555 0.0 36.205055 1 ATCAACG 9685 0.0 30.318304 3 TCAACGC 9805 0.0 29.897932 4 TATCAAC 9945 0.0 29.71677 2 CAACGCA 9965 0.0 29.417044 5 AACGCAG 10210 0.0 28.751085 6 ACGCAGA 11405 0.0 25.406858 7 CGCAGAG 11455 0.0 25.130085 8 GTACATG 7405 0.0 25.030394 1 TACATGG 7635 0.0 24.021177 2 ACATGGG 7485 0.0 23.867783 3 ACACCGT 260 2.3646862E-11 23.749884 6 GCAGAGT 12065 0.0 23.268938 9 TAGACCG 145 2.331191E-5 22.936182 5 AGAGTAC 10370 0.0 21.552172 10-11 GTGTAGC 1065 0.0 21.420015 1 AGTACTT 6515 0.0 21.14402 12-13 GAGTACT 6170 0.0 21.094507 12-13 GTACTTT 6580 0.0 19.92306 14-15 >>END_MODULE