FastQCFastQC Report
Thu 26 May 2016
SRR937283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748100
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83870.4797780447342829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67540.3863623362507866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48280.2761855729077284No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17550.10039471426119786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54300.036.756251
GTATCAA86100.034.3848081
ATCAACG106450.027.351763
TATCAAC108850.027.0105042
TCAACGC108000.026.9592114
CAACGCA110000.026.4690425
AACGCAG112350.025.9576726
GTACATG74700.024.1101721
ACGCAGA126700.022.8697247
TACATGG76500.022.7863672
CGCAGAG127500.022.6889728
ACATGGG75900.022.2155483
GAGTACT71650.021.21492612-13
AGTACTT74650.021.03048912-13
GCAGAGT136400.020.9299349
AGAGTAC114700.019.92000210-11
GTACTTT76600.019.56325114-15
GTATAGG5650.019.344661
CGCGGAA1851.4188117E-1017.97252882-83
TACTTTT86850.017.88332714-15
Produced by FastQC (version 0.11.5)