##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937283_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1748100 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14022309936502 33.0 31.0 34.0 31.0 34.0 2 32.337484697671755 34.0 31.0 34.0 31.0 34.0 3 32.40656598592758 34.0 31.0 34.0 31.0 34.0 4 36.064338996624905 37.0 35.0 37.0 35.0 37.0 5 35.98596533379097 37.0 35.0 37.0 35.0 37.0 6 36.026529946799386 37.0 35.0 37.0 35.0 37.0 7 35.964471712144615 37.0 35.0 37.0 35.0 37.0 8 35.94021909501745 37.0 35.0 37.0 35.0 37.0 9 37.62701904925348 39.0 37.0 39.0 35.0 39.0 10-11 37.54233110233969 39.0 37.0 39.0 35.0 39.0 12-13 37.42617384588982 39.0 37.0 39.0 34.5 39.0 14-15 38.62876523082204 40.0 38.0 41.0 34.0 41.0 16-17 38.576573708597905 40.0 38.0 41.0 34.0 41.0 18-19 38.56496910931869 40.0 38.0 41.0 34.0 41.0 20-21 38.460460499971404 40.0 38.0 41.0 34.0 41.0 22-23 38.407234997997826 40.0 38.0 41.0 34.0 41.0 24-25 38.442834792059955 40.0 38.0 41.0 34.0 41.0 26-27 38.29180538870774 40.0 38.0 41.0 34.0 41.0 28-29 38.12608575024312 40.0 38.0 41.0 33.5 41.0 30-31 38.16357445226245 40.0 38.0 41.0 34.0 41.0 32-33 38.067156627195246 40.0 37.0 41.0 33.5 41.0 34-35 37.930520851209884 40.0 37.0 41.0 33.0 41.0 36-37 37.75400634975116 40.0 37.0 41.0 33.0 41.0 38-39 37.63851924947085 40.0 37.0 41.0 33.0 41.0 40-41 37.44268834734855 40.0 37.0 41.0 33.0 41.0 42-43 37.23683856758767 40.0 36.5 41.0 32.0 41.0 44-45 36.930555174189124 39.0 36.0 41.0 31.0 41.0 46-47 36.57328327898861 39.0 36.0 41.0 30.5 41.0 48-49 36.23697814770322 39.0 35.5 40.0 30.0 41.0 50-51 35.97765459641897 39.0 35.0 40.0 29.0 41.0 52-53 35.72643984897889 39.0 35.0 40.0 28.5 41.0 54-55 35.505008294719985 38.5 35.0 40.0 28.0 41.0 56-57 35.19383073050741 38.0 34.5 40.0 27.5 41.0 58-59 34.90689205423031 38.0 34.0 40.0 27.0 41.0 60-61 34.54688804988273 38.0 34.0 40.0 26.0 41.0 62-63 34.146370058921114 37.0 33.5 40.0 26.0 41.0 64-65 33.71548681425548 36.5 33.0 39.0 25.0 40.5 66-67 33.2608340483954 36.0 32.0 39.0 23.5 40.0 68-69 32.792865682741265 35.5 32.0 38.5 22.5 40.0 70-71 32.337219266632346 35.0 31.0 38.0 22.0 40.0 72-73 31.83366712430639 35.0 31.0 37.5 20.5 39.0 74-75 31.326471311709856 34.5 30.5 37.0 19.0 39.0 76-77 30.034495166180427 33.5 29.5 35.5 18.0 37.5 78-79 30.694864996281677 34.0 30.0 36.0 18.0 38.0 80-81 30.496520507980094 34.0 30.0 35.5 17.5 37.0 82-83 30.06859762027344 34.0 30.0 35.0 13.0 37.0 84-85 29.594123333905383 34.0 29.0 35.0 7.5 36.0 86-87 29.164309250042905 34.0 29.0 35.0 4.5 36.0 88-89 28.659799782621132 33.5 29.0 35.0 2.0 35.5 90-91 28.035478805560324 33.0 27.0 35.0 2.0 35.0 92-93 27.473948572736113 33.0 26.0 35.0 2.0 35.0 94-95 26.900907556775927 32.0 25.5 34.0 2.0 35.0 96-97 26.13825582060523 32.0 24.5 34.0 2.0 35.0 98-99 25.03593444310966 32.0 20.5 34.0 2.0 35.0 100-101 21.98215462502145 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28911431290148926 1101 2 0.2864295287658152 1101 3 0.30682332942156165 1101 4 0.20089743540223992 1101 5 0.18766963974679385 1101 6 0.2221512748860306 1101 7 0.26775088370083466 1101 8 0.1425020553661014 1101 9 0.27062129441159044 1101 10-11 0.3099054342604859 1101 12-13 0.2692778347974141 1101 14-15 0.3874128136097639 1101 16-17 0.43995122549543453 1101 18-19 0.36426420362072776 1101 20-21 0.50315987132619 1101 22-23 0.39191740726568725 1101 24-25 0.3785162126291013 1101 26-27 0.40089395382152304 1101 28-29 0.33472639908328716 1101 30-31 0.5101029414613123 1101 32-33 0.46397899543138976 1101 34-35 0.5368486652142153 1101 36-37 0.5378765832295542 1101 38-39 0.4725385449913446 1101 40-41 0.6936680207729466 1101 42-43 0.7166406937482606 1101 44-45 0.6475834675859531 1101 46-47 0.7346864126703778 1101 48-49 0.6089570679022103 1101 50-51 0.6245058131377021 1101 52-53 0.7884301556176894 1101 54-55 0.8078383741219213 1101 56-57 0.8510949552902716 1101 58-59 0.9301845404378355 1101 60-61 0.8950736983247864 1101 62-63 0.871676611108704 1101 64-65 1.0179826054186805 1101 66-67 0.9010689484015657 1101 68-69 1.005480827099042 1101 70-71 0.9134031769015181 1101 72-73 0.7701565317365464 1101 74-75 0.8182391526871378 1101 76-77 0.937880981226666 1101 78-79 1.1820566467875295 1101 80-81 1.1758185105292824 1101 82-83 0.9190258538652181 1101 84-85 0.9533022750227076 1101 86-87 1.3730600123305017 1101 88-89 1.6499382806191036 1101 90-91 1.7838846717062644 1101 92-93 1.7445793037793464 1101 94-95 1.918801488521197 1101 96-97 2.2194654679849037 1101 98-99 2.524089819467637 1101 100-101 2.993108927049242 1102 1 0.25690542934806615 1102 2 0.28937070523640074 1102 3 0.2565009147414088 1102 4 0.16904355099133284 1102 5 0.12090064643518872 1102 6 0.1507364232310877 1102 7 0.21265227282790988 1102 8 0.2008436777293383 1102 9 0.23789963431898542 1102 10-11 0.30707401597888406 1102 12-13 0.32710325067805 1102 14-15 0.4418528753487436 1102 16-17 0.40673908349577914 1102 18-19 0.4377413087484925 1102 20-21 0.40569717196073185 1102 22-23 0.48533363089799764 1102 24-25 0.2804018240165149 1102 26-27 0.29287303116674224 1102 28-29 0.37903097805773456 1102 30-31 0.36125968987496293 1102 32-33 0.41599065723897866 1102 34-35 0.42540980395542505 1102 36-37 0.41076116563394294 1102 38-39 0.4291531908496893 1102 40-41 0.42313294960161585 1102 42-43 0.5097250703560476 1102 44-45 0.5228681528886412 1102 46-47 0.5418592818180343 1102 48-49 0.5752570164530582 1102 50-51 0.5548831069123565 1102 52-53 0.5127750364271577 1102 54-55 0.6641932017672687 1102 56-57 0.6268375380376554 1102 58-59 0.596404742804495 1102 60-61 0.8211644203278752 1102 62-63 0.7129405452537796 1102 64-65 0.8119862068592596 1102 66-67 0.7599970910872074 1102 68-69 0.7515904137908649 1102 70-71 0.7529684315748213 1102 72-73 0.8935779002839652 1102 74-75 0.8870309551224018 1102 76-77 0.8763134970901518 1102 78-79 0.7977357755819057 1102 80-81 0.8331311498707343 1102 82-83 0.8632781262027223 1102 84-85 0.7390800146899998 1102 86-87 1.0025969699706998 1102 88-89 1.1016875517561289 1102 90-91 1.3748056115107623 1102 92-93 1.4494138090100073 1102 94-95 1.4971705504316795 1102 96-97 1.5543711445412356 1102 98-99 2.002504328128243 1102 100-101 2.6458468793730283 1103 1 0.20632000185965182 1103 2 0.29024814451760506 1103 3 0.20191576803355815 1103 4 0.13168850396058218 1103 5 0.10024783160996975 1103 6 0.1370357353186904 1103 7 0.1643285328307229 1103 8 0.09242447233835094 1103 9 0.2609376800862151 1103 10-11 0.3009466819903963 1103 12-13 0.3204641941007651 1103 14-15 0.3248382747591094 1103 16-17 0.4683535717731928 1103 18-19 0.3464651610579139 1103 20-21 0.3854479792048693 1103 22-23 0.41948286577093796 1103 24-25 0.37686609468898524 1103 26-27 0.3579032150780179 1103 28-29 0.3095033913667322 1103 30-31 0.3626762278505353 1103 32-33 0.4479238314633207 1103 34-35 0.49170560628589755 1103 36-37 0.4611760189260963 1103 38-39 0.46659387595428115 1103 40-41 0.4406459357029746 1103 42-43 0.5415410186924206 1103 44-45 0.5945156328830237 1103 46-47 0.485822499563362 1103 48-49 0.41996289880599846 1103 50-51 0.26768459504938136 1103 52-53 0.2354494713562616 1103 54-55 0.4159459744753988 1103 56-57 0.44607241098444206 1103 58-59 0.2678297041971689 1103 60-61 0.3446685618412815 1103 62-63 0.3757855543791564 1103 64-65 0.5278847046832098 1103 66-67 0.43487705739354254 1103 68-69 0.5015454888996658 1103 70-71 0.4863616647630806 1103 72-73 0.22028925785802045 1103 74-75 0.2586089419257114 1103 76-77 0.2357758022986296 1103 78-79 0.4053273802428663 1103 80-81 0.5137193851648512 1103 82-83 0.6671135887887125 1103 84-85 0.7099476266512532 1103 86-87 0.6799534209042974 1103 88-89 0.9877257379136459 1103 90-91 1.3103587492336324 1103 92-93 1.424990111129901 1103 94-95 1.439858322437857 1103 96-97 1.8673670732229724 1103 98-99 2.059645981925801 1103 100-101 2.651880853822 1104 1 0.09313661949854435 1104 2 0.16201092411465368 1104 3 0.24084427999448366 1104 4 0.1478260407314167 1104 5 0.18020981196185915 1104 6 0.06331508260181096 1104 7 0.1578027516240823 1104 8 0.09459880768829265 1104 9 0.1319830816514056 1104 10-11 0.11413283950829367 1104 12-13 0.17600201948790328 1104 14-15 0.2190895374554529 1104 16-17 0.2059319699526867 1104 18-19 0.1060450023189432 1104 20-21 0.16928130328768276 1104 22-23 0.13061748862713074 1104 24-25 0.04301126314100401 1104 26-27 0.207024877951973 1104 28-29 0.15869582073899124 1104 30-31 0.20032261737154755 1104 32-33 0.27668833165758144 1104 34-35 0.2860630187297062 1104 36-37 0.14836034484051197 1104 38-39 0.27336317716980574 1104 40-41 0.1627396527479945 1104 42-43 0.2345198719976338 1104 44-45 0.15000905576142287 1104 46-47 0.11904806430777626 1104 48-49 0.3060094104339086 1104 50-51 0.192598565188689 1104 52-53 0.29775451659131136 1104 54-55 0.30496953555660156 1104 56-57 0.4475557322920949 1104 58-59 0.414181760588356 1104 60-61 0.4577957472772525 1104 62-63 0.40827570257253143 1104 64-65 0.42083025610686775 1104 66-67 0.44977479286559685 1104 68-69 0.5034679787703453 1104 70-71 0.637383616253338 1104 72-73 0.8033316622538678 1104 74-75 0.5143736363809488 1104 76-77 0.758940036077842 1104 78-79 0.7594491818746576 1104 80-81 0.5765100828392704 1104 82-83 0.646113283521462 1104 84-85 0.8665150352098365 1104 86-87 0.614782332487799 1104 88-89 0.8752340633840348 1104 90-91 1.089685912468351 1104 92-93 1.384653207094825 1104 94-95 1.397827185178599 1104 96-97 1.5809306520651774 1104 98-99 1.8300385278546472 1104 100-101 2.4385247179503224 1105 1 0.2271818087108386 1105 2 0.21198955174111234 1105 3 0.11376381300812 1105 4 0.0999953366452857 1105 5 0.12028267839788498 1105 6 0.10656775748793734 1105 7 0.016299939821188048 1105 8 0.04999967326942567 1105 9 0.14587679416626997 1105 10-11 0.15827413761283537 1105 12-13 0.2231210903239642 1105 14-15 0.3026434960197619 1105 16-17 0.32704587134590213 1105 18-19 0.3975137519015206 1105 20-21 0.35364240289629834 1105 22-23 0.3184157000266268 1105 24-25 0.2549342090555342 1105 26-27 0.28632473235267497 1105 28-29 0.31501503049548063 1105 30-31 0.25405743964689975 1105 32-33 0.31594470663587515 1105 34-35 0.36813710739833994 1105 36-37 0.45451458658877186 1105 38-39 0.436051187268248 1105 40-41 0.37366611174643083 1105 42-43 0.27770408849977457 1105 44-45 0.4179995851283991 1105 46-47 0.2790463278024262 1105 48-49 0.2846240131081217 1105 50-51 0.34077323091140954 1105 52-53 0.39480412721339064 1105 54-55 0.5166500386088373 1105 56-57 0.43411918820489603 1105 58-59 0.3242518219003472 1105 60-61 0.34136089294334226 1105 62-63 0.414894459501852 1105 64-65 0.42910804353189747 1105 66-67 0.5798311958951317 1105 68-69 0.6455710537205874 1105 70-71 0.6253394489076598 1105 72-73 0.4061234285469624 1105 74-75 0.5816663911463174 1105 76-77 0.6143038339486182 1105 78-79 0.4498331165286231 1105 80-81 0.46891754714072675 1105 82-83 0.39632049536175984 1105 84-85 0.45449407692603927 1105 86-87 0.6193348150225617 1105 88-89 0.786782324875027 1105 90-91 0.8999595122439423 1105 92-93 0.9797435721616807 1105 94-95 1.031843321320622 1105 96-97 1.3416622551267707 1105 98-99 1.7050807716867133 1105 100-101 2.1746705777208355 1106 1 -0.07259281038943044 1106 2 0.10246671644739536 1106 3 0.04117500982976452 1106 4 0.1540994350532685 1106 5 0.09993164756312467 1106 6 0.09755051900303613 1106 7 0.023513263360094072 1106 8 -0.01698214173267587 1106 9 -0.07356770683013991 1106 10-11 -0.2104749466625364 1106 12-13 -0.3063301156958076 1106 14-15 -0.09138976743759741 1106 16-17 -0.14866919486495078 1106 18-19 -0.012879568306566114 1106 20-21 0.052999868751243184 1106 22-23 0.07133458793391867 1106 24-25 0.13860789435864973 1106 26-27 0.16983888549220438 1106 28-29 0.20850495009860737 1106 30-31 0.1446255510363912 1106 32-33 0.1739337136015564 1106 34-35 -0.28117665398626457 1106 36-37 0.13708546409242928 1106 38-39 0.10461410618619738 1106 40-41 0.15105549805156215 1106 42-43 0.18224361292705282 1106 44-45 0.17204971627518262 1106 46-47 0.24852862590662994 1106 48-49 0.4794486628966439 1106 50-51 0.47461821304080587 1106 52-53 0.33436012717466923 1106 54-55 0.49974930799825756 1106 56-57 0.5680589551344113 1106 58-59 0.49739561678337907 1106 60-61 0.6510053131056708 1106 62-63 0.5407297922715983 1106 64-65 0.34376851536661945 1106 66-67 0.31863365669544663 1106 68-69 0.4646589102190646 1106 70-71 0.5043361384483873 1106 72-73 0.4002980014563349 1106 74-75 0.4941378013472324 1106 76-77 0.2805688889621827 1106 78-79 0.476791215305731 1106 80-81 0.545968077785421 1106 82-83 0.5997977625290716 1106 84-85 0.6637445394191275 1106 86-87 0.7531964846167689 1106 88-89 0.8842267731390443 1106 90-91 0.8537125397672476 1106 92-93 1.0300020443041262 1106 94-95 0.6504162694049143 1106 96-97 1.0418734516814112 1106 98-99 1.2827639241223672 1106 100-101 1.7718063078858606 1107 1 0.1456672465749591 1107 2 0.24111702876581376 1107 3 0.13849014793468228 1107 4 0.07101177578124407 1107 5 0.06724781030073501 1107 6 0.12982255768486795 1107 7 0.06679880644135494 1107 8 0.1218205684856386 1107 9 0.2027368191929355 1107 10-11 0.177302482365441 1107 12-13 0.21409379689654173 1107 14-15 0.1445294772395087 1107 16-17 0.2537335687898974 1107 18-19 0.24545270580731682 1107 20-21 0.23168916250694593 1107 22-23 0.26992396703244736 1107 24-25 0.1733515351509638 1107 26-27 0.24480908683901959 1107 28-29 0.2513011986459688 1107 30-31 0.22457770458084525 1107 32-33 0.19659333581040528 1107 34-35 0.18879609597222924 1107 36-37 0.2353620059700816 1107 38-39 0.23958255009339524 1107 40-41 0.28533620590413733 1107 42-43 0.39235702938966455 1107 44-45 0.1645750856617525 1107 46-47 0.23913475450711275 1107 48-49 0.16103432737742907 1107 50-51 0.1697852865342071 1107 52-53 0.06358845554480297 1107 54-55 0.08150478264962402 1107 56-57 0.07439465463428974 1107 58-59 0.09146711150197717 1107 60-61 0.2719607701177935 1107 62-63 0.18961005680841225 1107 64-65 0.41280881715337614 1107 66-67 0.3630223011712488 1107 68-69 0.4599918055975891 1107 70-71 0.4841028853563287 1107 72-73 0.5576804475528689 1107 74-75 0.40808859692912236 1107 76-77 0.28465120717418557 1107 78-79 0.4046980917583767 1107 80-81 0.4710296530219935 1107 82-83 0.4733739770430567 1107 84-85 0.5474873151101676 1107 86-87 0.8436743071555739 1107 88-89 0.8876808553275595 1107 90-91 0.9382168510065583 1107 92-93 0.9624797228755568 1107 94-95 1.2230607815241186 1107 96-97 1.4683995058857775 1107 98-99 1.6707803260274012 1107 100-101 2.0856164434486573 1108 1 0.11859783815516778 1108 2 0.11883176340268875 1108 3 0.04299122036086089 1108 4 0.06668403735347539 1108 5 -0.023203855700863585 1108 6 0.0355737548117645 1108 7 0.0925208236001751 1108 8 0.0671368095071827 1108 9 0.011478339544090943 1108 10-11 0.05128367749712481 1108 12-13 0.08854261972175692 1108 14-15 0.09761363775427156 1108 16-17 0.0691708942088951 1108 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46-47 0.060788293213171585 2316 48-49 -0.11374128967567998 2316 50-51 -0.3776346017417822 2316 52-53 -0.14410747050231976 2316 54-55 0.08247623225695122 2316 56-57 0.0773849664330939 2316 58-59 -0.46737643982562815 2316 60-61 -0.0020362264229731863 2316 62-63 0.22563842021528302 2316 64-65 0.17257444215337614 2316 66-67 0.2548977686357219 2316 68-69 0.19375471930364796 2316 70-71 0.18623452335184254 2316 72-73 0.331288864250709 2316 74-75 0.5500853129912002 2316 76-77 0.5070649599789725 2316 78-79 0.46804683194162067 2316 80-81 0.5893083904004541 2316 82-83 0.6864472053527102 2316 84-85 0.7106645542649943 2316 86-87 0.7604203453941665 2316 88-89 0.9223681800283785 2316 90-91 1.1077837451726005 2316 92-93 0.9733920966601488 2316 94-95 1.3536411386669727 2316 96-97 1.5473893151602702 2316 98-99 1.8235214208291985 2316 100-101 2.624452515344693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 13.0 5 33.0 6 60.0 7 173.0 8 367.0 9 772.0 10 1365.0 11 2347.0 12 3436.0 13 4982.0 14 6773.0 15 9407.0 16 12641.0 17 17485.0 18 15698.0 19 9921.0 20 9051.0 21 9387.0 22 10356.0 23 11310.0 24 13094.0 25 15756.0 26 19409.0 27 23838.0 28 29769.0 29 37435.0 30 46481.0 31 58746.0 32 75003.0 33 98753.0 34 133330.0 35 187548.0 36 273144.0 37 365627.0 38 230578.0 39 14003.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.15147372149946 20.086312128050068 15.892545349348937 21.869668801101536 2 15.501076026630086 20.846256903217096 40.19351317832295 23.45915389182986 3 19.324866998455466 25.008351924947085 30.178765516846862 25.488015559750586 4 12.982438075624964 17.479320405011155 37.50809450260282 32.030147016761056 5 13.440509907721667 37.77879857969968 34.33936985757525 14.4413216550034 6 31.45661003375093 37.18522967793604 17.214804645043188 14.143355643269837 7 28.144328127681483 33.12350552027916 20.693553000400435 18.038613351638922 8 26.237343401407244 35.597791888335905 19.48366798238087 18.681196727875978 9 26.205880670442195 16.973399691093185 20.301698987472115 36.51902065099251 10-11 24.70350666437847 27.313054173102223 27.291430696184428 20.692008466334876 12-13 25.842372665129297 24.439319248356274 28.101743183244484 21.61656490326995 14-15 22.52617127166638 25.768977747268462 26.640123562725243 25.06472741833991 16-17 21.61009095589497 29.337566500772265 27.141839711686977 21.910502831645786 18-19 21.561295120416453 27.41839711686974 30.027057948629942 20.99324981408386 20-21 22.26305390201236 26.38381829851644 30.3817634938087 20.971364305662497 22-23 21.961614126495704 26.41003815018783 30.24793903749862 21.380408685817844 24-25 21.84646812152283 26.5883034689959 30.32195821805338 21.24327019142789 26-27 21.737496331662346 26.885010700390776 30.11735248134082 21.260140486606062 28-29 21.420084391468407 26.914282478015426 30.36997352469389 21.29565960582228 30-31 21.655761468477706 26.62777101486417 30.286094127553636 21.43037338910449 32-33 21.530692653012775 26.774336099674073 30.162762660549543 21.53220858676361 34-35 21.99250614953378 26.757965791430692 29.96953835592929 21.27998970310623 36-37 22.046450431897487 26.578542417481838 29.93793261255077 21.437074538069904 38-39 21.723642812196097 26.820118986328012 30.0851209885018 21.371117212974085 40-41 21.936502488415993 26.696213031291116 30.009267204393343 21.35801727589955 42-43 21.847047980718237 26.7791035673892 29.703147588664123 21.670700863228436 44-45 21.83762370573766 26.959956524226303 29.467478977175222 21.734940792860822 46-47 22.002831645786856 27.155854928207766 29.093015273725758 21.74829815227962 48-49 22.19023511240776 27.36333733768091 28.783822435787425 21.662605114123906 50-51 22.14713117098564 27.417510439906184 28.616240489674503 21.81911789943367 52-53 22.292466761760416 27.575118006726733 28.40679263394332 21.725622597569533 54-55 22.13188604770894 27.416366340598366 28.514901893484357 21.93684571820834 56-57 22.171300268863337 27.69203706881757 28.423002116583717 21.71366054573537 58-59 22.29803786968709 27.41118929123048 28.425690749957095 21.865082089125337 60-61 22.212802471254506 27.31665808592186 28.868771809393056 21.601767633430583 62-63 22.190177907442365 27.38616211887192 28.753503804130197 21.67015616955552 64-65 21.996167267318803 27.353097648875924 28.711629769463993 21.939105314341283 66-67 21.96047136891482 27.65534008351925 28.45180481665809 21.93238373090784 68-69 22.00717922315657 27.443052456953264 28.610491390652708 21.939276929237458 70-71 22.024569532635432 27.433956867456093 28.559235741662377 21.982237858246094 72-73 22.111956157989127 27.364988744890866 28.46650210657888 22.05655299054113 74-75 22.071935243979176 27.719266632343686 28.11320862650878 22.095589497168355 76-77 22.115067787883987 27.440564040958755 28.408786682684056 22.0355814884732 78-79 22.058606487043075 27.385361249356443 28.411675533436302 22.144356730164176 80-81 21.946999599565242 27.775499113323036 28.221983868199757 22.05551741891196 82-83 22.033721841855893 27.663934836512077 28.266733711058695 22.035609610573335 84-85 22.029460557176364 27.548681425547738 28.139551513071336 22.282306504204566 86-87 22.052628568159715 27.461215033464903 28.284737715233682 22.201418683141696 88-89 22.113437446370344 27.34122761855729 28.21820834048395 22.32712659458841 90-91 22.008024293712264 27.401452679779993 28.33909820806411 22.25142481844363 92-93 22.048160963299352 27.207406087437295 28.40223434285304 22.342198606410307 94-95 21.853583891081747 27.208998341056002 28.38639093873348 22.551026829128766 96-97 21.866369200846634 27.179566386362335 28.361020536582576 22.593043876208455 98-99 21.75875302078575 27.137203237921938 28.189290465371908 22.914753275920408 100-101 21.711599402486055 26.965963331042982 28.670628734104582 22.65180853236639 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 270.0 1 198.5 2 195.5 3 272.5 4 232.5 5 211.0 6 413.5 7 1123.5 8 2014.0 9 2429.0 10 2237.5 11 1993.5 12 2083.5 13 2217.0 14 2385.0 15 2543.5 16 2669.5 17 2820.5 18 3041.5 19 3273.0 20 3517.5 21 3967.0 22 4679.0 23 5590.5 24 6760.0 25 8366.0 26 10459.5 27 12856.5 28 15389.0 29 18396.5 30 21918.5 31 25707.5 32 29430.5 33 32997.5 34 37118.0 35 40795.5 36 44555.0 37 47830.0 38 50556.5 39 52857.5 40 54693.5 41 57840.0 42 60782.5 43 63854.0 44 66329.0 45 67298.5 46 66448.5 47 67318.0 48 71214.5 49 69843.5 50 65512.0 51 62166.5 52 58262.0 53 56146.5 54 54239.5 55 49856.0 56 44233.0 57 38583.5 58 33784.5 59 29494.0 60 24761.5 61 19813.5 62 15399.0 63 11671.0 64 8642.5 65 6584.5 66 4840.5 67 3626.0 68 2621.5 69 1676.5 70 1157.0 71 835.0 72 623.0 73 508.5 74 411.5 75 314.5 76 207.0 77 107.0 78 57.0 79 46.0 80 27.0 81 13.5 82 8.5 83 4.0 84 2.0 85 1.0 86 0.0 87 1.0 88 1.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04124478004690807 2 1.1440993078199187E-4 3 0.0 4 0.0 5 0.0010868943424289228 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005205651850580631 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.1440993078199187E-4 22-23 5.148446885189635E-4 24-25 2.8602482695497967E-5 26-27 0.0018877638579028661 28-29 0.0032034780618957724 30-31 2.2881986156398374E-4 32-33 1.4301241347748986E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.007265030604656484 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.8602482695497967E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.860248269549797E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.5742234425948175E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.009038384531777358 92-93 0.0014015216520794004 94-95 0.0 96-97 0.0 98-99 2.0021737886848578E-4 100-101 0.010926148389680224 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1748100.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.65766873178884 #Duplication Level Percentage of deduplicated Percentage of total 1 76.92162499684126 44.35121572378761 2 13.351727781624653 15.396589948598722 3 4.290697725138523 7.4217488419283075 4 1.8517430820773402 4.270687568111878 5 0.9628880419105796 2.7758939873140505 6 0.5731023503564154 1.9826247279755866 7 0.39124394192905515 1.5790749517945284 8 0.2722590127312515 1.2558255972241903 9 0.2029980056774266 1.053395258810657 >10 1.0649844806069049 11.217773301404936 >50 0.07825087115523963 3.0891767984386616 >100 0.03668558511865721 3.8321979441585543 >500 0.0011960832217959654 0.39676925642387745 >1k 3.9869440726532173E-4 0.5068807708080242 >5k 1.9934720363266086E-4 0.8701453232204415 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8387 0.4797780447342829 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6754 0.3863623362507866 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4828 0.2761855729077284 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1755 0.10039471426119786 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.720496539099594E-5 0.0 0.0 0.0 0.0 8 5.720496539099594E-5 0.0 0.0 0.0 0.0 9 5.720496539099594E-5 0.0 0.0 0.0 0.0 10-11 5.720496539099594E-5 0.0 0.0 2.860248269549797E-5 0.0 12-13 5.720496539099594E-5 0.0 0.0 5.720496539099594E-5 0.0 14-15 1.1440993078199188E-4 0.0 0.0 5.720496539099594E-5 0.0 16-17 1.1440993078199188E-4 0.0 0.0 5.720496539099594E-5 0.0 18-19 1.1440993078199188E-4 0.0 0.0 5.720496539099594E-5 0.0 20-21 1.1440993078199188E-4 0.0 0.0 1.4301241347748986E-4 0.0 22-23 1.1440993078199188E-4 0.0 0.0 2.860248269549797E-4 0.0 24-25 1.1440993078199188E-4 0.0 0.0 4.0043475773697155E-4 0.0 26-27 1.1440993078199188E-4 0.0 0.0 8.580744808649391E-4 0.0 28-29 1.7161489617298782E-4 0.0 0.0 0.0012299067559064128 0.0 30-31 1.7161489617298782E-4 0.0 0.0 0.002688633373376809 0.0 32-33 1.7161489617298782E-4 0.0 0.0 0.004719409644757165 0.0 34-35 1.7161489617298782E-4 0.0 0.0 0.006721583433442023 0.0 36-37 1.7161489617298782E-4 0.0 0.0 0.010068073908815285 0.0 38-39 1.7161489617298782E-4 0.0 0.0 0.016560837480693324 0.0 40-41 1.7161489617298782E-4 0.0 0.0 0.023339625879526343 0.0 42-43 2.2881986156398376E-4 0.0 0.0 0.028631085178193467 0.0 44-45 2.860248269549797E-4 0.0 0.0 0.03372232709799211 0.0 46-47 2.860248269549797E-4 0.0 0.0 0.04021509066987014 2.860248269549797E-5 48-49 2.860248269549797E-4 0.0 0.0 0.047365711343744635 5.720496539099594E-5 50-51 2.860248269549797E-4 0.0 0.0 0.054745151879183114 5.720496539099594E-5 52-53 2.860248269549797E-4 0.0 0.0 0.06941822550197357 8.580744808649391E-5 54-55 2.860248269549797E-4 0.0 0.0 0.09207139179680796 1.1440993078199188E-4 56-57 2.860248269549797E-4 0.0 0.0 0.12705222813340197 1.1440993078199188E-4 58-59 2.860248269549797E-4 0.0 0.0 0.17330244265202221 1.1440993078199188E-4 60-61 2.860248269549797E-4 0.0 0.0 0.22638865053486643 1.1440993078199188E-4 62-63 2.860248269549797E-4 0.0 0.0 0.2705222813340198 1.1440993078199188E-4 64-65 3.1462730965047767E-4 0.0 0.0 0.3254676505920714 1.1440993078199188E-4 66-67 4.0043475773697155E-4 0.0 0.0 0.4108174589554373 1.1440993078199188E-4 68-69 4.0043475773697155E-4 0.0 0.0 0.5197929180252846 1.1440993078199188E-4 70-71 4.2903724043246957E-4 0.0 0.0 0.6393512956924661 1.7161489617298782E-4 72-73 5.148446885189635E-4 0.0 0.0 0.7665751387220411 2.860248269549797E-4 74-75 5.720496539099594E-4 0.0 0.0 0.8998913105657571 2.860248269549797E-4 76-77 5.720496539099594E-4 0.0 0.0 1.0426463016989875 2.860248269549797E-4 78-79 5.720496539099594E-4 0.0 0.0 1.189605857788456 2.860248269549797E-4 80-81 5.720496539099594E-4 0.0 0.0 1.3372518734626166 2.860248269549797E-4 82-83 6.006521366054573E-4 0.0 0.0 1.5148446885189633 2.860248269549797E-4 84-85 6.864595846919513E-4 0.0 0.0 1.7507579657914307 2.860248269549797E-4 86-87 7.436645500829472E-4 0.0 0.0 2.028488072764716 2.860248269549797E-4 88-89 7.436645500829472E-4 0.0 0.0 2.3588467478977178 2.860248269549797E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5430 0.0 36.75625 1 GTATCAA 8610 0.0 34.384808 1 ATCAACG 10645 0.0 27.35176 3 TATCAAC 10885 0.0 27.010504 2 TCAACGC 10800 0.0 26.959211 4 CAACGCA 11000 0.0 26.469042 5 AACGCAG 11235 0.0 25.957672 6 GTACATG 7470 0.0 24.110172 1 ACGCAGA 12670 0.0 22.869724 7 TACATGG 7650 0.0 22.786367 2 CGCAGAG 12750 0.0 22.688972 8 ACATGGG 7590 0.0 22.215548 3 GAGTACT 7165 0.0 21.214926 12-13 AGTACTT 7465 0.0 21.030489 12-13 GCAGAGT 13640 0.0 20.929934 9 AGAGTAC 11470 0.0 19.920002 10-11 GTACTTT 7660 0.0 19.563251 14-15 GTATAGG 565 0.0 19.34466 1 CGCGGAA 185 1.4188117E-10 17.972528 82-83 TACTTTT 8685 0.0 17.883327 14-15 >>END_MODULE