FastQCFastQC Report
Thu 26 May 2016
SRR937282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709042
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66680.3901601013901355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47250.2764706777247136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34380.20116533122064875No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22880.13387617156278195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46400.036.3849331
GTATCAA74900.035.1119161
ATCAACG94700.027.7082543
TCAACGC95250.027.4500564
CAACGCA96050.027.1208485
TATCAAC97250.027.0305922
AACGCAG98600.026.5414986
GTACATG73250.026.2291951
TACATGG76650.024.6205882
ACATGGG76750.023.7833423
GTGTAGC9100.023.5170211
ACGCAGA112200.023.0280287
CGCAGAG112550.022.9564178
GTAGCCC8400.022.0701543
CATGGGG33400.021.3496284
GAGTACT60400.021.23103512-13
GCAGAGT120050.021.2057369
AGTACTT63550.021.11286712-13
AGAGTAC100950.020.58365210-11
GTACTTT64250.020.29087814-15
Produced by FastQC (version 0.11.5)