FastQCFastQC Report
Thu 26 May 2016
SRR937282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709042
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85020.4974716829662466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67040.39226654464899047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48280.28249744593754866No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17850.10444447825155848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54350.037.423051
GTATCAA86500.034.611421
ATCAACG108500.027.3178463
TATCAAC109250.027.2172642
TCAACGC110250.026.9273134
CAACGCA111500.026.6680375
AACGCAG114650.025.9338176
CGCAGAG130200.022.8033568
GTACATG70250.022.7970431
ACGCAGA130550.022.7422227
GAGTACT75050.021.8372612-13
TACATGG72150.021.5279672
AGTACTT77550.021.34768112-13
GCAGAGT137750.021.2431459
ACATGGG71700.021.00063
GTACTTT78850.020.72158414-15
CGCGGAA2000.020.18681582-83
AGAGTAC116900.020.07438310-11
GTGTAGG8050.020.0714151
GTGTAGC9750.019.9836221
Produced by FastQC (version 0.11.5)