##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937282_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1709042 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11289599670459 31.0 31.0 34.0 31.0 34.0 2 32.338273137816394 34.0 31.0 34.0 31.0 34.0 3 32.42199548050896 34.0 31.0 34.0 31.0 34.0 4 36.05876157519827 37.0 35.0 37.0 35.0 37.0 5 35.91371657337854 37.0 35.0 37.0 35.0 37.0 6 36.00712036333806 37.0 35.0 37.0 35.0 37.0 7 35.968918844592466 37.0 35.0 37.0 35.0 37.0 8 35.93945145877047 37.0 35.0 37.0 35.0 37.0 9 37.63754723406446 39.0 37.0 39.0 35.0 39.0 10-11 37.55496471122419 39.0 37.0 39.0 35.0 39.0 12-13 37.46795836497874 39.0 37.0 39.0 35.0 39.0 14-15 38.66707342476077 40.0 38.0 41.0 34.0 41.0 16-17 38.64992668407213 40.0 38.0 41.0 34.5 41.0 18-19 38.65976114103691 40.0 38.0 41.0 34.0 41.0 20-21 38.57570264510761 40.0 38.0 41.0 34.0 41.0 22-23 38.451721198191734 40.0 38.0 41.0 34.0 41.0 24-25 38.30212803430226 40.0 38.0 41.0 34.0 41.0 26-27 38.09067243520054 40.0 37.5 41.0 33.5 41.0 28-29 38.08387242677476 40.0 38.0 41.0 33.0 41.0 30-31 38.20086340768688 40.0 38.0 41.0 34.0 41.0 32-33 38.16206477078972 40.0 38.0 41.0 34.0 41.0 34-35 38.049613467662 40.0 37.0 41.0 33.5 41.0 36-37 37.892966351909436 40.0 37.0 41.0 33.0 41.0 38-39 37.751031572073714 40.0 37.0 41.0 33.0 41.0 40-41 37.58541217828468 40.0 37.0 41.0 33.0 41.0 42-43 37.38269129722968 40.0 37.0 41.0 32.5 41.0 44-45 37.097959558629924 40.0 36.5 41.0 31.5 41.0 46-47 36.76676202223234 39.5 36.0 41.0 31.0 41.0 48-49 36.45375508618278 39.0 36.0 41.0 30.0 41.0 50-51 36.23815067154581 39.0 36.0 41.0 30.0 41.0 52-53 36.0073950786464 39.0 35.0 40.0 29.5 41.0 54-55 35.806589890710704 39.0 35.0 40.0 29.0 41.0 56-57 35.569585182810016 38.5 35.0 40.0 28.0 41.0 58-59 35.30814514798349 38.0 35.0 40.0 28.0 41.0 60-61 34.97565800021299 38.0 34.0 40.0 27.0 41.0 62-63 34.605516423821065 37.5 34.0 40.0 26.5 41.0 64-65 34.26519711042795 37.0 34.0 40.0 26.0 41.0 66-67 33.881293438078174 36.5 33.0 39.0 26.0 40.5 68-69 33.44995588171619 36.0 33.0 39.0 25.0 40.0 70-71 33.06496622084185 35.5 32.5 38.5 24.5 40.0 72-73 32.61976739015191 35.0 32.0 38.0 23.5 40.0 74-75 32.17290212879496 35.0 31.5 37.0 22.5 39.0 76-77 30.874133578929015 34.0 30.0 36.0 21.0 38.5 78-79 31.50914986290565 34.5 31.0 36.0 22.0 39.0 80-81 31.334046793466747 34.5 31.0 36.0 21.0 37.5 82-83 30.99394280538454 34.0 31.0 35.5 20.0 37.0 84-85 30.646063116061512 34.0 31.0 35.0 19.0 37.0 86-87 30.30222311681047 34.0 31.0 35.0 18.0 36.0 88-89 29.92594769467339 34.0 30.0 35.0 14.0 36.0 90-91 29.468582691355742 34.0 30.0 35.0 7.0 35.5 92-93 29.04400974347032 34.0 29.0 35.0 2.0 35.0 94-95 28.625848867377165 34.0 29.0 35.0 2.0 35.0 96-97 28.177575507213984 33.5 29.0 35.0 2.0 35.0 98-99 27.502793670372057 33.0 27.5 35.0 2.0 35.0 100-101 25.814899516805323 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2043066401419722 1101 2 0.17984179603111272 1101 3 0.20651725745992877 1101 4 0.1907936113648745 1101 5 0.018281387176386943 1101 6 0.1127007432241669 1101 7 0.13707760791532309 1101 8 0.10694806669278734 1101 9 0.2414496233533825 1101 10-11 0.29091348774939263 1101 12-13 0.34769936290225445 1101 14-15 0.34710389026840716 1101 16-17 0.2987107252209995 1101 18-19 0.44901377099236583 1101 20-21 0.28346325452571364 1101 22-23 0.3102509004739957 1101 24-25 0.3118690489813716 1101 26-27 0.2645544858909119 1101 28-29 0.2888428701206891 1101 30-31 0.5152015228787903 1101 32-33 0.3236091695054668 1101 34-35 0.306825661447931 1101 36-37 0.2257705247168147 1101 38-39 0.3412223738888329 1101 40-41 0.423552400378405 1101 42-43 0.29109541953574336 1101 44-45 0.38507104374891554 1101 46-47 0.3151897444668492 1101 48-49 0.3757052286172069 1101 50-51 0.3796735316107487 1101 52-53 0.34830151590085023 1101 54-55 0.37171147315294917 1101 56-57 0.42426862066115234 1101 58-59 0.4597338748126063 1101 60-61 0.37169179228963856 1101 62-63 0.44345774255913284 1101 64-65 0.46848641814880665 1101 66-67 0.3544770015255949 1101 68-69 0.3266864504809064 1101 70-71 0.16814539867391431 1101 72-73 0.2039049048708108 1101 74-75 0.255627455476791 1101 76-77 0.2735632512351849 1101 78-79 0.3884430615324561 1101 80-81 0.2441070432645418 1101 82-83 0.28780449193994784 1101 84-85 0.37616942088764915 1101 86-87 0.27886507872097965 1101 88-89 0.17625518795682282 1101 90-91 0.4938121659216783 1101 92-93 0.4970817115708961 1101 94-95 0.35940995418034305 1101 96-97 0.17645490117415363 1101 98-99 0.15926016645325447 1101 100-101 0.540182341521593 1102 1 0.21627540795025624 1102 2 0.21040112359050056 1102 3 0.1477403108064692 1102 4 0.15144212791918932 1102 5 0.045579072292639466 1102 6 0.08784070667066857 1102 7 0.157294978014356 1102 8 0.1301382963329516 1102 9 0.26702872933285704 1102 10-11 0.2409509974396613 1102 12-13 0.18892379598933218 1102 14-15 0.29336347786075123 1102 16-17 0.16220689788493559 1102 18-19 0.2441807003445149 1102 20-21 0.21696810351151186 1102 22-23 0.2709951966908051 1102 24-25 0.3289564937275813 1102 26-27 0.14905480162688178 1102 28-29 0.21344550484754166 1102 30-31 0.3671511925111517 1102 32-33 0.31482968797987354 1102 34-35 0.2867448214875594 1102 36-37 0.34925714187539114 1102 38-39 0.4728269992924794 1102 40-41 0.44154229169335224 1102 42-43 0.5890372317062074 1102 44-45 0.5412390614917442 1102 46-47 0.49937499805673724 1102 48-49 0.6530897090390155 1102 50-51 0.6486099891044717 1102 52-53 0.6690976069354875 1102 54-55 0.722727571837126 1102 56-57 0.7402550324630113 1102 58-59 0.6329423298625798 1102 60-61 0.6833894571269212 1102 62-63 0.651526304753979 1102 64-65 0.5607681175270827 1102 66-67 0.5819793695453868 1102 68-69 0.6623725088124814 1102 70-71 0.4475744620418638 1102 72-73 0.6158914167840663 1102 74-75 0.48655607219385644 1102 76-77 0.4606268681832759 1102 78-79 0.6279182082341705 1102 80-81 0.6840400392138832 1102 82-83 0.7612501019264464 1102 84-85 0.8379106598561421 1102 86-87 0.8056880368333381 1102 88-89 0.7571350966244204 1102 90-91 0.6054769736821086 1102 92-93 0.616253430868781 1102 94-95 0.517754271959614 1102 96-97 0.5684377423358562 1102 98-99 0.5717390532144186 1102 100-101 0.5715513842511939 1103 1 0.22171388765082867 1103 2 0.18373282022940884 1103 3 0.1783985531290071 1103 4 0.20407927938268955 1103 5 0.09660754283478923 1103 6 0.09471581585715683 1103 7 0.11015817859456689 1103 8 0.03644206240652892 1103 9 0.12040583431599572 1103 10-11 0.17581844504338306 1103 12-13 0.22425637803139864 1103 14-15 0.28440770434396967 1103 16-17 0.20856748063638264 1103 18-19 0.2057152932708135 1103 20-21 0.06477074198644317 1103 22-23 0.14780984303246214 1103 24-25 0.22763854664530214 1103 26-27 0.17341265273091722 1103 28-29 0.059604390377749894 1103 30-31 0.12147927871686193 1103 32-33 0.352376108780625 1103 34-35 0.31883423397161437 1103 36-37 0.32219527085573674 1103 38-39 0.32624628996411786 1103 40-41 0.29903671987379 1103 42-43 0.291054733307611 1103 44-45 0.20420816634782568 1103 46-47 0.17549826232244925 1103 48-49 0.05351841489458309 1103 50-51 -0.09065135437900551 1103 52-53 0.186467522815569 1103 54-55 0.06256604862605997 1103 56-57 0.12837544332183626 1103 58-59 -0.09174857311629125 1103 60-61 0.07261959974432841 1103 62-63 0.26614812908152885 1103 64-65 0.07277151752814603 1103 66-67 0.10354452807093395 1103 68-69 0.15573099253641942 1103 70-71 0.2522247807340605 1103 72-73 0.18491299335754974 1103 74-75 0.16267040301965352 1103 76-77 -0.05840036623548173 1103 78-79 0.2698801636913757 1103 80-81 0.221282392189309 1103 82-83 0.14617234129498868 1103 84-85 0.12016682988531358 1103 86-87 0.2713579852708463 1103 88-89 0.2317034129555431 1103 90-91 0.34020228033070765 1103 92-93 0.35783018963149615 1103 94-95 0.38901628909064456 1103 96-97 0.3001465241058163 1103 98-99 0.30221849439135795 1103 100-101 0.6083288674827969 1104 1 0.13806826435681074 1104 2 0.20707028140927974 1104 3 0.14658063654608355 1104 4 0.09342749025297081 1104 5 0.027521655531479894 1104 6 0.13166479697063949 1104 7 0.013309821844892156 1104 8 0.11438347724213571 1104 9 0.09657465243356711 1104 10-11 0.143912676647993 1104 12-13 0.13461689463895965 1104 14-15 0.24937638890084912 1104 16-17 0.2619595007093878 1104 18-19 0.293505512219582 1104 20-21 0.16353546770076832 1104 22-23 0.09023870251494515 1104 24-25 0.11118448568716843 1104 26-27 0.03985471916115557 1104 28-29 -0.06020927301041468 1104 30-31 0.05116443856825725 1104 32-33 0.08472518844374122 1104 34-35 0.14920680994235624 1104 36-37 0.1054285417310794 1104 38-39 0.0567584238878851 1104 40-41 0.06225922809166207 1104 42-43 0.08303785019915466 1104 44-45 0.06558448979809839 1104 46-47 0.13734853171306582 1104 48-49 -0.04190243094655699 1104 50-51 0.03336671521518042 1104 52-53 0.16250034892085097 1104 54-55 0.13547819033006192 1104 56-57 -0.027827510573786185 1104 58-59 0.03542016719894292 1104 60-61 0.0791611559208576 1104 62-63 0.09695396685066981 1104 64-65 -0.03996569857434196 1104 66-67 -0.05506900175653584 1104 68-69 0.09173159550458365 1104 70-71 0.2038296161496902 1104 72-73 0.2158598478736522 1104 74-75 0.06207083871277064 1104 76-77 0.09852631153963287 1104 78-79 0.038887438210458924 1104 80-81 -0.029799384556866926 1104 82-83 -0.019599166190243267 1104 84-85 0.02619472850885529 1104 86-87 -0.07762231776927564 1104 88-89 0.15106420187130354 1104 90-91 0.3009548883300823 1104 92-93 0.10422632026162759 1104 94-95 -0.03593800203275421 1104 96-97 0.024982815008776527 1104 98-99 0.15684368160324524 1104 100-101 0.2376405760870881 1105 1 0.10165274936498037 1105 2 0.06058484254833019 1105 3 -0.023466106040338275 1105 4 0.11646183148651801 1105 5 -0.005972131124714508 1105 6 7.526198301732734E-4 1105 7 0.04986657798539795 1105 8 0.07224484557118416 1105 9 0.0026505553001925364 1105 10-11 0.10903244660858746 1105 12-13 -0.02061504486169241 1105 14-15 0.17071361956094933 1105 16-17 0.10943521103202869 1105 18-19 0.14337187149146047 1105 20-21 -0.0146225070404995 1105 22-23 -0.010781653944775371 1105 24-25 -0.07147293802361077 1105 26-27 0.13473868607398742 1105 28-29 0.06110357296706326 1105 30-31 0.059345657929064544 1105 32-33 -0.00919605853191996 1105 34-35 -0.04265092285218941 1105 36-37 -0.1956108157800358 1105 38-39 -0.10425965244566271 1105 40-41 0.03421240950103055 1105 42-43 -0.04873000762231783 1105 44-45 0.0308740024844667 1105 46-47 0.2051683510764022 1105 48-49 0.07779564234683534 1105 50-51 0.12999244517224895 1105 52-53 0.1584610420792032 1105 54-55 0.26876106531580746 1105 56-57 0.18050920072423082 1105 58-59 0.07850346780823969 1105 60-61 -0.00273717578682664 1105 62-63 0.020072546078722553 1105 64-65 0.1492657722337043 1105 66-67 0.018682629167038556 1105 68-69 0.184807295693318 1105 70-71 -0.005857436533894145 1105 72-73 0.1225824198559664 1105 74-75 0.05524091079008997 1105 76-77 0.09218502732190359 1105 78-79 0.03257117204373472 1105 80-81 -0.0338922110265969 1105 82-83 -0.06880048866342392 1105 84-85 -0.14576763674202198 1105 86-87 0.07753488426256894 1105 88-89 0.20456736237951745 1105 90-91 0.016906270973258586 1105 92-93 0.11089442256328752 1105 94-95 0.3887744487228524 1105 96-97 0.3894872675884855 1105 98-99 0.5131080115628386 1105 100-101 0.18848992308428691 1106 1 0.04240080504148125 1106 2 0.08448428116571449 1106 3 0.05189544198395879 1106 4 0.07795866313797717 1106 5 -0.04257583384467267 1106 6 -0.004428705660487253 1106 7 0.05171973871292579 1106 8 0.041427004550058655 1106 9 0.025099428579757443 1106 10-11 -0.01378776625246303 1106 12-13 -0.12392079676646262 1106 14-15 0.043748957274807765 1106 16-17 0.1324093555329 1106 18-19 0.08157347917190094 1106 20-21 -0.0937292854992009 1106 22-23 -0.007419885972204554 1106 24-25 0.07786776379148108 1106 26-27 0.22850771851379648 1106 28-29 0.13085772609865387 1106 30-31 0.159156836832544 1106 32-33 0.25684537406453956 1106 34-35 0.2214542252881273 1106 36-37 0.22454024109717352 1106 38-39 0.18502008768093958 1106 40-41 0.09412017914186777 1106 42-43 -0.0853322633141218 1106 44-45 0.03458402190037191 1106 46-47 0.027197714814903406 1106 48-49 0.028861812693556033 1106 50-51 0.15907304849937987 1106 52-53 0.36572579871157984 1106 54-55 0.31716512188238966 1106 56-57 0.2601941473224656 1106 58-59 0.2905165669502523 1106 60-61 0.303893396638216 1106 62-63 0.4166947643278718 1106 64-65 0.265730155082629 1106 66-67 0.36122265235807305 1106 68-69 0.39693599630705023 1106 70-71 0.3868406021319899 1106 72-73 0.35369002022666507 1106 74-75 0.41673523425941283 1106 76-77 0.2596087576833206 1106 78-79 0.40587388840499017 1106 80-81 0.528858325820849 1106 82-83 0.4579968650114843 1106 84-85 0.4786901296510422 1106 86-87 0.681430702093941 1106 88-89 0.5072340423374051 1106 90-91 0.4699166040405487 1106 92-93 0.48855687398333814 1106 94-95 0.37791225146525775 1106 96-97 0.3829913974068475 1106 98-99 0.6235054876884831 1106 100-101 0.7737959309522644 1107 1 -0.01473035463329353 1107 2 0.03910434306673949 1107 3 0.057284683197870834 1107 4 0.007153411027900347 1107 5 -0.03582302355451361 1107 6 -0.0034448176397603447 1107 7 0.053772679754587216 1107 8 0.008225661482519797 1107 9 -0.07728314746639597 1107 10-11 -0.008964020640007675 1107 12-13 0.07957260960437651 1107 14-15 0.1729160807355683 1107 16-17 0.07654885766140751 1107 18-19 0.14827377827138122 1107 20-21 -0.03622572250528577 1107 22-23 0.11684266254861342 1107 24-25 0.10793844145093345 1107 26-27 0.08300548347449421 1107 28-29 -0.014964729341464533 1107 30-31 0.12763603148753333 1107 32-33 0.10084988192541999 1107 34-35 0.12297158687022147 1107 36-37 0.1423193175121682 1107 38-39 0.1704080963250334 1107 40-41 0.2209466587056781 1107 42-43 0.11921982143217491 1107 44-45 0.06990380563701137 1107 46-47 0.13441998555537538 1107 48-49 0.020135961616482234 1107 50-51 0.08298028863928408 1107 52-53 0.08778765265763155 1107 54-55 0.04929316916352633 1107 56-57 0.13562361119174682 1107 58-59 0.05635338585477001 1107 60-61 -0.00943514267983403 1107 62-63 -0.00917761948073803 1107 64-65 -0.012476647835647725 1107 66-67 -0.07323715069089332 1107 68-69 -0.024013076261582 1107 70-71 -0.16243354431340862 1107 72-73 -0.12340926605966729 1107 74-75 -0.01300967953192611 1107 76-77 -0.016795334448485022 1107 78-79 0.06953469510025911 1107 80-81 0.07064956565601932 1107 82-83 0.0902954281075985 1107 84-85 0.01685674707685081 1107 86-87 0.15668546932096206 1107 88-89 0.12023669391097513 1107 90-91 0.26077567423695314 1107 92-93 0.49615436623022546 1107 94-95 0.2482392479031965 1107 96-97 0.21570705222040587 1107 98-99 0.252681437340172 1107 100-101 0.6200131803643139 1108 1 7.337152656887724E-4 1108 2 -0.0028361836768979742 1108 3 0.013961070689269661 1108 4 -0.027879645460892277 1108 5 -0.0457473602929781 1108 6 0.026688033631621977 1108 7 0.07677237846586848 1108 8 0.08122564442844293 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 15.0 5 30.0 6 64.0 7 127.0 8 373.0 9 726.0 10 1395.0 11 2131.0 12 3377.0 13 4688.0 14 6241.0 15 8787.0 16 11889.0 17 16367.0 18 14385.0 19 8454.0 20 7307.0 21 7396.0 22 7698.0 23 8661.0 24 10146.0 25 11935.0 26 15001.0 27 18614.0 28 23256.0 29 29665.0 30 37735.0 31 47774.0 32 61945.0 33 81413.0 34 113225.0 35 162664.0 36 252134.0 37 388212.0 38 316917.0 39 28289.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.13031884533089 20.084416591782553 15.923123530978605 21.86214103190795 2 15.547482156670227 20.849107277644435 40.156415114432534 23.446995451252807 3 19.279455975921014 25.001199502411293 30.257887167196596 25.4614573544711 4 12.95796124378453 17.54544358769416 37.5673623000488 31.92923286847251 5 13.443495734903339 37.792086120017245 34.35186155069634 14.412556594383075 6 31.451070248712437 37.18714929182548 17.216838439312784 14.144942020149301 7 28.149805563584746 33.114282738516664 20.688725028407728 18.047186669490863 8 26.32117876564766 35.50486178806606 19.45756745591975 18.71639199036653 9 26.168754190944398 17.03147143253355 20.266851253509277 36.53292312301278 10-11 24.72031114507426 27.305209585253028 27.293653403485695 20.68082586618702 12-13 25.814188912425244 24.448049668215386 28.095837185623836 21.641924233735534 14-15 22.51638637318451 25.75741263234022 26.69159096148603 25.034610032989242 16-17 21.61702287012256 29.34105773877997 27.134441400503906 21.90747799059356 18-19 21.47875827510383 27.430455190685777 30.127287685147586 20.96349884906281 20-21 22.23476661193815 26.373488773242553 30.417830574087706 20.973914040731593 22-23 21.932429676782505 26.413888197296753 30.264312373264318 21.38936975265642 24-25 21.812149161674316 26.616215090242633 30.378579914998642 21.193055833084408 26-27 21.67860728888972 26.85970022445717 30.181268475587064 21.280424011066042 28-29 21.410613981681106 26.936826525211664 30.38975120253037 21.26280829057686 30-31 21.581061989311472 26.63524920737132 30.357451616517807 21.4262371867994 32-33 21.50020303772523 26.734451230572446 30.214383262669962 21.55096246903236 34-35 21.892528094686963 26.73076495486945 30.097826735679988 21.278880214763593 36-37 21.974887063757155 26.56998273006563 30.03678381921541 21.418346386961797 38-39 21.727785376271733 26.80131360487994 30.125962712930527 21.344938305917804 40-41 21.897982552788374 26.681308406293354 30.067944537316542 21.35276450360173 42-43 21.811114879069503 26.76859075397628 29.779293543887757 21.64100082306646 44-45 21.858779280309832 26.92691398275407 29.532702843290476 21.68160389364562 46-47 21.92754771386543 27.14046816871674 29.164877165101856 21.767106952315977 48-49 22.14478115364665 27.374379147609933 28.821886419961128 21.65895327878229 50-51 22.147056655131937 27.408688610344274 28.66763953138659 21.776615203137194 52-53 22.275192242653468 27.494970854948807 28.50905012059416 21.720786781803564 54-55 22.07435510654507 27.393212103622965 28.58940856924523 21.943024220586736 56-57 22.11323653836477 27.721700227378847 28.446521501519566 21.71854173273682 58-59 22.27227300440832 27.388735911697896 28.46556725931838 21.873423824575404 60-61 22.19535856930374 27.298363644661745 28.966491168736635 21.539786617297878 62-63 22.1715733141725 27.412404142203645 28.8265004604919 21.589522083131953 64-65 21.989453740750665 27.39865374870834 28.71544409090005 21.896448419640947 66-67 21.981174248497112 27.622521857274425 28.48244806154559 21.91385583268287 68-69 21.93609051152634 27.473754302117793 28.68174099875837 21.908414187597497 70-71 22.02151848813546 27.41012216200655 28.612052834277918 21.956306515580074 72-73 22.132896675447412 27.357987691349887 28.487216815034387 22.021898818168307 74-75 22.081259559449094 27.659355358148012 28.17505362653463 22.08433145586826 76-77 22.08696450994183 27.40236928056771 28.511236119416612 21.999430090073854 78-79 21.99161869632227 27.44716045597475 28.449096043280385 22.112124804422596 80-81 21.958383702682553 27.750195723686133 28.305009473143432 21.986411100487878 82-83 22.025805220961082 27.658343836342635 28.349933354163813 21.96591758853247 84-85 21.99461569383063 27.54846138857991 28.21661387877763 22.240309038811827 86-87 22.05156456073051 27.443591204897245 28.28028802100826 22.22455621336398 88-89 22.026199473155135 27.42688593960827 28.25852144066676 22.28839314656983 90-91 21.97541028692875 27.428513581044466 28.400586099105517 22.19549003292126 92-93 21.98647107923292 27.24239331002738 28.45069837399587 22.320437236743825 94-95 21.818627043688803 27.213198973459985 28.38906241040302 22.57911157244819 96-97 21.914675005061316 27.20410030882799 28.360479145626616 22.520745540484082 98-99 21.7192347436136 27.24757200316906 28.164529186868283 22.86866406634906 100-101 21.47034521178982 27.281632710385377 28.531000711583836 22.717021366240964 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 285.0 1 199.5 2 181.5 3 271.0 4 234.5 5 216.0 6 445.5 7 1140.5 8 1970.0 9 2372.0 10 2252.5 11 2045.5 12 2075.0 13 2205.5 14 2413.0 15 2608.0 16 2756.5 17 2917.5 18 3075.0 19 3309.0 20 3521.5 21 3906.0 22 4679.5 23 5502.5 24 6601.0 25 8198.5 26 10313.0 27 12780.0 28 15106.0 29 17850.0 30 21373.5 31 24931.0 32 28747.5 33 32641.0 34 36789.0 35 40165.0 36 43543.0 37 47151.5 38 49358.0 39 51143.0 40 53183.5 41 56495.5 42 59642.0 43 62318.0 44 64803.5 45 66004.0 46 65436.0 47 65561.0 48 69160.5 49 68392.5 50 64013.5 51 60947.5 52 56919.5 53 54453.5 54 52489.0 55 48450.5 56 43253.5 57 37798.5 58 33143.0 59 28787.0 60 24102.5 61 19191.5 62 14837.5 63 11338.0 64 8446.5 65 6421.0 66 4650.5 67 3503.0 68 2474.5 69 1546.5 70 1109.5 71 827.5 72 613.5 73 485.5 74 369.0 75 283.0 76 204.0 77 110.5 78 62.0 79 36.5 80 19.5 81 10.0 82 4.0 83 2.0 84 2.5 85 1.5 86 0.5 87 1.5 88 1.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04955992889583755 2 0.0 3 0.0 4 0.0 5 0.010005605479561063 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005968255900089056 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0013165270367843506 24-25 6.143792838326969E-4 26-27 0.0017553693823791341 28-29 0.0024282609789578018 30-31 7.899162220706103E-4 32-33 0.0 34-35 0.0 36-37 1.4628078186492782E-4 38-39 2.633054073568701E-4 40-41 5.851231274597113E-5 42-43 0.011029570952615558 44-45 5.851231274597113E-5 46-47 0.0 48-49 2.9256156372985564E-5 50-51 0.0 52-53 2.340492509838845E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.68098501967769E-4 84-85 5.851231274597113E-5 86-87 0.0 88-89 0.0 90-91 0.0067874282785326525 92-93 0.0021356994152279462 94-95 0.0 96-97 0.0 98-99 0.0011702462549194226 100-101 0.010415191668782861 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1709042.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.894714011760875 #Duplication Level Percentage of deduplicated Percentage of total 1 77.18000230578569 44.68314160920507 2 13.186471997520446 15.268540502410788 3 4.2672943932834855 7.411613655094142 4 1.8124042264253455 4.197144974504083 5 0.9624099967636225 2.785922576234482 6 0.5676000023017138 1.9716623883799522 7 0.39370045763762723 1.5955222780860867 8 0.2671092840322248 1.2371372487145489 9 0.1960764482494669 1.0216610906260246 >10 1.0513773713961787 11.15353262091776 >50 0.07858518498135421 3.13672895934262 >100 0.03524673385388987 3.756146730696947 >500 0.00111397502702209 0.36960504216204904 >1k 4.050818280080327E-4 0.5201088719891398 >5k 2.0254091400401635E-4 0.89153145163644 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8502 0.4974716829662466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6704 0.39226654464899047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4828 0.28249744593754866 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1785 0.10444447825155848 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 5.8512312745971134E-5 0.0 0.0 0.0 0.0 14-15 1.1702462549194227E-4 0.0 0.0 0.0 0.0 16-17 1.1702462549194227E-4 0.0 0.0 0.0 5.8512312745971134E-5 18-19 1.1702462549194227E-4 0.0 0.0 0.0 5.8512312745971134E-5 20-21 1.1702462549194227E-4 0.0 0.0 0.0 5.8512312745971134E-5 22-23 1.1702462549194227E-4 0.0 0.0 1.4628078186492782E-4 8.77684691189567E-5 24-25 1.755369382379134E-4 0.0 0.0 3.8033003284881233E-4 1.1702462549194227E-4 26-27 1.755369382379134E-4 0.0 0.0 6.143792838326969E-4 1.1702462549194227E-4 28-29 1.755369382379134E-4 0.0 0.0 0.0010239654730544948 1.1702462549194227E-4 30-31 1.755369382379134E-4 0.0 0.0 0.0024575171353307876 1.1702462549194227E-4 32-33 1.755369382379134E-4 0.0 0.0 0.004300654986828878 1.1702462549194227E-4 34-35 2.340492509838845E-4 0.0 0.0 0.006202305151072941 1.1702462549194227E-4 36-37 2.9256156372985565E-4 0.0 0.0 0.00950825082122031 1.1702462549194227E-4 38-39 2.9256156372985565E-4 0.0 0.0 0.014569565873746812 1.1702462549194227E-4 40-41 2.9256156372985565E-4 0.0 0.0 0.019192038580678535 1.1702462549194227E-4 42-43 2.9256156372985565E-4 0.0 0.0 0.024779964447918773 1.1702462549194227E-4 44-45 2.9256156372985565E-4 0.0 0.0 0.029139131747493625 1.1702462549194227E-4 46-47 3.2181772010284125E-4 0.0 0.0 0.03589730386965329 1.1702462549194227E-4 48-49 3.510738764758268E-4 0.0 0.0 0.041338948955028605 1.1702462549194227E-4 50-51 3.510738764758268E-4 0.0 0.0 0.046751337884030936 1.1702462549194227E-4 52-53 3.510738764758268E-4 0.0 0.0 0.06149644069601566 1.1702462549194227E-4 54-55 3.510738764758268E-4 0.0 0.0 0.08197575015710556 1.1702462549194227E-4 56-57 3.510738764758268E-4 0.0 0.0 0.11333834978894608 1.1702462549194227E-4 58-59 3.510738764758268E-4 0.0 0.0 0.15391663867827707 1.1702462549194227E-4 60-61 3.510738764758268E-4 0.0 0.0 0.21026399585264727 1.1702462549194227E-4 62-63 3.510738764758268E-4 0.0 0.0 0.24990608773804274 1.1702462549194227E-4 64-65 3.510738764758268E-4 0.0 0.0 0.2991734550701504 1.1702462549194227E-4 66-67 3.510738764758268E-4 0.0 0.0 0.38799514581853456 1.1702462549194227E-4 68-69 3.510738764758268E-4 0.0 0.0 0.49615515592946224 1.1702462549194227E-4 70-71 3.510738764758268E-4 0.0 0.0 0.6089961510600677 1.1702462549194227E-4 72-73 3.510738764758268E-4 0.0 0.0 0.7363189435953008 1.1702462549194227E-4 74-75 3.510738764758268E-4 0.0 0.0 0.8690833812159092 1.1702462549194227E-4 76-77 3.510738764758268E-4 0.0 0.0 1.003018065091437 1.1702462549194227E-4 78-79 3.510738764758268E-4 0.0 0.0 1.1405220000444694 1.1702462549194227E-4 80-81 4.0958618922179793E-4 0.0 0.0 1.2798398166926268 1.1702462549194227E-4 82-83 4.0958618922179793E-4 0.0 0.0 1.4630126117438893 1.1702462549194227E-4 84-85 4.3884234559478353E-4 0.0 0.0 1.698817232110153 1.1702462549194227E-4 86-87 4.6809850196776907E-4 0.0 0.0 1.9802322002619013 1.1702462549194227E-4 88-89 4.6809850196776907E-4 0.0 0.0 2.306877186166285 1.1702462549194227E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5435 0.0 37.42305 1 GTATCAA 8650 0.0 34.61142 1 ATCAACG 10850 0.0 27.317846 3 TATCAAC 10925 0.0 27.217264 2 TCAACGC 11025 0.0 26.927313 4 CAACGCA 11150 0.0 26.668037 5 AACGCAG 11465 0.0 25.933817 6 CGCAGAG 13020 0.0 22.803356 8 GTACATG 7025 0.0 22.797043 1 ACGCAGA 13055 0.0 22.742222 7 GAGTACT 7505 0.0 21.83726 12-13 TACATGG 7215 0.0 21.527967 2 AGTACTT 7755 0.0 21.347681 12-13 GCAGAGT 13775 0.0 21.243145 9 ACATGGG 7170 0.0 21.0006 3 GTACTTT 7885 0.0 20.721584 14-15 CGCGGAA 200 0.0 20.186815 82-83 AGAGTAC 11690 0.0 20.074383 10-11 GTGTAGG 805 0.0 20.071415 1 GTGTAGC 975 0.0 19.983622 1 >>END_MODULE