FastQCFastQC Report
Thu 26 May 2016
SRR937281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783941
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68100.3817390821781662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50440.2827447768732262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34830.19524188299949383No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25600.14350250372630036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83000.035.636391
GGTATCA51050.034.372571
ATCAACG99650.029.4310683
TCAACGC100350.029.1792244
CAACGCA100950.029.0025355
TATCAAC101500.028.9430982
AACGCAG104150.028.096426
GTACATG77400.026.0499131
TACATGG80150.024.8503842
ACGCAGA117850.024.7093017
CGCAGAG119250.024.4192128
ACATGGG79300.023.8565733
GAGTACT60800.023.04651812-13
GCAGAGT124500.022.7770759
AGTACTT64550.021.19254512-13
AGAGTAC105100.021.03826510-11
CGCGGAA1259.516953E-920.89791582-83
GTACTTT67650.020.466314-15
CATGGGA45800.019.5120454
CATGGGG35600.018.6934054
Produced by FastQC (version 0.11.5)