##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937281_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1783941 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55805937528203 31.0 31.0 34.0 30.0 34.0 2 31.6536651156064 33.0 31.0 34.0 30.0 34.0 3 31.70360903191305 33.0 31.0 34.0 30.0 34.0 4 35.34497833728806 37.0 35.0 37.0 33.0 37.0 5 35.325631284891145 37.0 35.0 37.0 33.0 37.0 6 35.39242385258257 37.0 35.0 37.0 33.0 37.0 7 35.32473439424286 37.0 35.0 37.0 33.0 37.0 8 35.311133608118205 37.0 35.0 37.0 33.0 37.0 9 36.91103741659618 39.0 37.0 39.0 33.0 39.0 10-11 36.811767317416894 39.0 37.0 39.0 33.0 39.0 12-13 36.6936927846829 39.0 37.0 39.0 32.0 39.0 14-15 37.8211437485881 40.0 38.0 41.0 33.0 41.0 16-17 37.76102600926824 40.0 37.5 41.0 33.0 41.0 18-19 37.70529826939344 40.0 37.5 41.0 32.0 41.0 20-21 37.64029724077197 40.0 37.0 41.0 32.0 41.0 22-23 37.50088259645358 40.0 37.0 41.0 32.0 41.0 24-25 37.3523132211211 40.0 37.0 41.0 32.0 41.0 26-27 37.190833385184824 39.0 36.5 41.0 31.5 41.0 28-29 36.99830767945801 39.0 36.0 41.0 31.0 41.0 30-31 37.00600580400361 39.0 36.0 41.0 31.0 41.0 32-33 36.86448935250661 39.0 36.0 41.0 30.5 41.0 34-35 36.66355361528212 39.0 36.0 41.0 30.0 41.0 36-37 36.66029061499231 39.0 36.0 41.0 30.0 41.0 38-39 36.63816264102905 39.0 36.0 41.0 30.0 41.0 40-41 36.47592717472158 39.0 35.5 41.0 30.0 41.0 42-43 36.24055251827275 39.0 35.0 41.0 30.0 41.0 44-45 36.02384551955474 39.0 35.0 41.0 29.0 41.0 46-47 35.71265865855429 39.0 35.0 40.5 27.5 41.0 48-49 35.419636916243306 39.0 35.0 40.0 26.5 41.0 50-51 34.512861411896466 38.0 34.0 39.5 25.0 40.5 52-53 34.66206785986756 38.0 34.0 39.5 26.0 40.5 54-55 35.01309236123841 38.0 35.0 40.0 26.0 41.0 56-57 34.79602632598275 38.0 34.0 40.0 25.5 41.0 58-59 34.55005014179281 38.0 34.0 40.0 24.5 41.0 60-61 34.2661982655256 38.0 34.0 40.0 23.5 41.0 62-63 33.942408128968395 37.5 33.5 40.0 23.0 41.0 64-65 33.57724274513563 37.0 33.0 40.0 22.0 41.0 66-67 33.185607595766896 36.5 32.5 39.0 21.5 41.0 68-69 32.685554903441314 36.0 32.0 39.0 19.5 40.5 70-71 32.260938001873384 35.0 31.5 39.0 18.0 40.0 72-73 31.836598575849763 35.0 31.0 38.0 17.5 40.0 74-75 31.370573914720275 35.0 31.0 37.0 13.5 39.0 76-77 30.921656321593595 35.0 30.5 37.0 9.5 39.0 78-79 30.43804755874774 34.5 30.0 36.0 7.0 39.0 80-81 29.979513335923105 34.0 29.5 36.0 4.5 37.5 82-83 29.523925679156427 34.0 29.0 35.0 2.0 37.0 84-85 29.043582999661986 34.0 29.0 35.0 2.0 36.5 86-87 28.58048920900411 34.0 29.0 35.0 2.0 36.0 88-89 28.184969962571632 34.0 27.5 35.0 2.0 36.0 90-91 27.729133418650058 33.5 26.5 35.0 2.0 35.0 92-93 27.263366053025297 33.0 26.0 35.0 2.0 35.0 94-95 26.696544336387806 33.0 25.0 35.0 2.0 35.0 96-97 26.02680694036406 32.0 24.5 35.0 2.0 35.0 98-99 25.264481000212452 32.0 21.0 34.0 2.0 35.0 100-101 23.384395840445396 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08018727890633315 1101 2 0.1320141157739485 1101 3 0.1166856798027105 1101 4 0.1696142995111316 1101 5 0.16723902629548348 1101 6 -0.010409341330877453 1101 7 0.05541893602173076 1101 8 0.06977368463648048 1101 9 0.13760628959553145 1101 10-11 0.1214791538631701 1101 12-13 0.09673413792575758 1101 14-15 0.1700297881111652 1101 16-17 0.12449667780219897 1101 18-19 0.27063659764232995 1101 20-21 0.208875669200296 1101 22-23 0.1693341313011132 1101 24-25 0.16414278010488914 1101 26-27 0.2997828471736099 1101 28-29 0.2776119663847538 1101 30-31 0.22293958202623543 1101 32-33 0.14396413539110853 1101 34-35 0.16172367544245958 1101 36-37 0.393279756774497 1101 38-39 0.3461870773430249 1101 40-41 0.27510709597719085 1101 42-43 0.4308820783962872 1101 44-45 0.4197632604556176 1101 46-47 0.44251150541978745 1101 48-49 0.5149959847198318 1101 50-51 0.37153571328899915 1101 52-53 0.4633336574945588 1101 54-55 0.4253607977894589 1101 56-57 0.4887062181948778 1101 58-59 0.4304544826797496 1101 60-61 0.526861298781057 1101 62-63 0.6129280659831764 1101 64-65 0.5961408954353047 1101 66-67 0.6227240280669619 1101 68-69 0.4758130510109808 1101 70-71 0.4194958442749339 1101 72-73 0.2838842671265631 1101 74-75 0.48127234848140077 1101 76-77 0.4520837465820229 1101 78-79 0.3872641745322234 1101 80-81 0.4948413026387861 1101 82-83 0.58188789666821 1101 84-85 0.7838030292852878 1101 86-87 0.8881839378534053 1101 88-89 0.7292288343081275 1101 90-91 0.8147658478675801 1101 92-93 0.6749921476679539 1101 94-95 0.8573781110499077 1101 96-97 0.8249632217130376 1101 98-99 0.8271612169287295 1101 100-101 1.3460800232484864 1102 1 0.0839178757088419 1102 2 -0.08084375592627424 1102 3 -0.006650165989690038 1102 4 -0.019509382160201483 1102 5 0.029534474085572526 1102 6 0.11101621574668741 1102 7 0.0509831910376235 1102 8 0.14285025125168715 1102 9 0.11570470500669217 1102 10-11 -0.027969219901962106 1102 12-13 0.16805893169766506 1102 14-15 0.26318643148584187 1102 16-17 0.2255140287665398 1102 18-19 0.15894184552245605 1102 20-21 0.32824426914072546 1102 22-23 0.3591284685802947 1102 24-25 0.3995490471355936 1102 26-27 0.38724386681607825 1102 28-29 0.41873698216885913 1102 30-31 0.37648747316527675 1102 32-33 0.24921762431494443 1102 34-35 0.19561382889243362 1102 36-37 0.3702153225471889 1102 38-39 0.49089332208853165 1102 40-41 0.48305217540158907 1102 42-43 0.19593051346821255 1102 44-45 0.2687241791698156 1102 46-47 0.2612294854386903 1102 48-49 0.3962999899674955 1102 50-51 0.5742320727628041 1102 52-53 0.4937247642892686 1102 54-55 0.4027966558492224 1102 56-57 0.3774615191785742 1102 58-59 0.3166061942162486 1102 60-61 0.48066719437173333 1102 62-63 0.46764219603097956 1102 64-65 0.5089889592471692 1102 66-67 0.46414142090228694 1102 68-69 0.47680763687664296 1102 70-71 0.3505073974875259 1102 72-73 0.2722437733248775 1102 74-75 0.2862334903475592 1102 76-77 0.2559163814157408 1102 78-79 0.5719672579583666 1102 80-81 0.633291944215653 1102 82-83 0.5111476770374956 1102 84-85 0.5489969330378095 1102 86-87 0.6542542529145727 1102 88-89 0.7762084695926532 1102 90-91 0.7861751034548519 1102 92-93 0.8351402677776925 1102 94-95 0.8140404416501283 1102 96-97 0.8072346386769631 1102 98-99 1.0461001293808785 1102 100-101 1.1160509984975135 1103 1 0.26476327360117935 1103 2 0.14508436676771552 1103 3 0.24273304384714578 1103 4 0.2425152443460945 1103 5 0.14803177000035106 1103 6 0.0781087729920813 1103 7 0.22935163677981762 1103 8 0.31503907523647 1103 9 0.40794974998971867 1103 10-11 0.38151891918240466 1103 12-13 0.4587548430947024 1103 14-15 0.4858253528070051 1103 16-17 0.5278846689910139 1103 18-19 0.5241845811956409 1103 20-21 0.35488085118413437 1103 22-23 0.41670380896093917 1103 24-25 0.4468507329644211 1103 26-27 0.36304922490152336 1103 28-29 0.30901895059216855 1103 30-31 0.20419509667685531 1103 32-33 0.2898125794387312 1103 34-35 0.4343817202578464 1103 36-37 0.48606846907457424 1103 38-39 0.4440509465050795 1103 40-41 0.5691824895514017 1103 42-43 0.5499926942245992 1103 44-45 0.4058036603154065 1103 46-47 0.3436584651854204 1103 48-49 0.2858032376291888 1103 50-51 0.3121292626326948 1103 52-53 0.14279334067377647 1103 54-55 0.17983461511125398 1103 56-57 0.359730452448332 1103 58-59 0.25561273107931015 1103 60-61 0.16035853076574824 1103 62-63 0.33695196370248226 1103 64-65 0.3190791567416724 1103 66-67 0.5137120637378345 1103 68-69 0.4431061926901094 1103 70-71 0.513240453672033 1103 72-73 0.5167629826110165 1103 74-75 0.5153813837958907 1103 76-77 0.42074185094029914 1103 78-79 0.6413400175969812 1103 80-81 0.525475688490225 1103 82-83 0.6056602548094325 1103 84-85 0.4617481628350575 1103 86-87 0.5832928969880022 1103 88-89 0.6838839947952238 1103 90-91 0.6883205095661111 1103 92-93 0.8583959644264212 1103 94-95 0.6429717056345368 1103 96-97 0.9981161731292403 1103 98-99 1.3583278404462504 1103 100-101 1.437637452524502 1104 1 0.18846255850024463 1104 2 0.23787874245352114 1104 3 0.26317007508215084 1104 4 0.06812094660620005 1104 5 0.18792299788621136 1104 6 0.24444259456343786 1104 7 0.23382178798491537 1104 8 0.2799214476330505 1104 9 0.3131635862111537 1104 10-11 0.23741743806330362 1104 12-13 0.277722523420195 1104 14-15 0.2712871485975086 1104 16-17 0.317641120291988 1104 18-19 0.3911027916912815 1104 20-21 0.3936884371762872 1104 22-23 0.41459492591472724 1104 24-25 0.40031986905311356 1104 26-27 0.4290185847619057 1104 28-29 0.4649553859341822 1104 30-31 0.3620393873881156 1104 32-33 0.41214437723365194 1104 34-35 0.4106541447484702 1104 36-37 0.31540433525881895 1104 38-39 0.43613118971041587 1104 40-41 0.3725199011542486 1104 42-43 0.37908938149391247 1104 44-45 0.4320458426131708 1104 46-47 0.48496522138815124 1104 48-49 0.45601938786993657 1104 50-51 0.3985852503374261 1104 52-53 0.33199163936333775 1104 54-55 0.3899769162941453 1104 56-57 0.6713998009513773 1104 58-59 0.38727602820907236 1104 60-61 0.3568890571853416 1104 62-63 0.46726764171894786 1104 64-65 0.5860676829035896 1104 66-67 0.5937429427629013 1104 68-69 0.6535485680331377 1104 70-71 0.5361100665338157 1104 72-73 0.6182951656747413 1104 74-75 0.5464069976566428 1104 76-77 0.6014085056724774 1104 78-79 0.4983141079173947 1104 80-81 0.497705695847543 1104 82-83 0.6065355322496337 1104 84-85 0.6346616055998382 1104 86-87 0.6339245787738115 1104 88-89 0.7023000375668715 1104 90-91 0.7338851200082246 1104 92-93 0.6598871495828895 1104 94-95 0.617894309978908 1104 96-97 0.7996389064247929 1104 98-99 0.9721636510177234 1104 100-101 1.0931066819369981 1105 1 0.17962947761285264 1105 2 0.20202533335030637 1105 3 0.12258837155940938 1105 4 0.17259395861375992 1105 5 0.26980552775038547 1105 6 0.1752096950039146 1105 7 0.22583407867267624 1105 8 0.13534434228805736 1105 9 0.33159219145635177 1105 10-11 0.23340594571326534 1105 12-13 0.2579023969548402 1105 14-15 0.39850396691225143 1105 16-17 0.395443308913201 1105 18-19 0.3047778837089865 1105 20-21 0.3875048782919208 1105 22-23 0.36364071120210895 1105 24-25 0.3075115613898447 1105 26-27 0.4013085298839272 1105 28-29 0.5255984191224385 1105 30-31 0.33605983783006366 1105 32-33 0.18067176903645787 1105 34-35 0.33312131775103637 1105 36-37 0.6063770850217125 1105 38-39 0.47402066944486165 1105 40-41 0.4294740261312384 1105 42-43 0.39075377640497067 1105 44-45 0.3736553285981614 1105 46-47 0.48679985098884515 1105 48-49 0.4545457121281089 1105 50-51 0.3152244032330316 1105 52-53 0.4511506617400016 1105 54-55 0.4020248536896176 1105 56-57 0.465698184317489 1105 58-59 0.6777892696304235 1105 60-61 0.7749827009627097 1105 62-63 0.8070061396991193 1105 64-65 0.8276565915790144 1105 66-67 0.9185375177795763 1105 68-69 0.8762075896362163 1105 70-71 0.6672901056152298 1105 72-73 0.9399342953628498 1105 74-75 0.8232483961091681 1105 76-77 0.8270093089611343 1105 78-79 1.100480295220418 1105 80-81 0.8750451270783337 1105 82-83 1.0173477480545579 1105 84-85 0.9753087542500118 1105 86-87 0.9443473728630174 1105 88-89 0.913574237179148 1105 90-91 1.1046220197657846 1105 92-93 0.8356181469016484 1105 94-95 1.0320745517390861 1105 96-97 1.1267178722370623 1105 98-99 1.2150342321178513 1105 100-101 1.3565753046342302 1106 1 0.12497186193214915 1106 2 0.11549288164676952 1106 3 0.10430263450073696 1106 4 0.15715038170306883 1106 5 0.15597100271127573 1106 6 0.06523988340745035 1106 7 0.1669962491823611 1106 8 0.06803715452548431 1106 9 0.12647676813789133 1106 10-11 0.17676665562230198 1106 12-13 0.03138326268783942 1106 14-15 0.0722455355800733 1106 16-17 -0.029523895653916554 1106 18-19 0.07646521288214814 1106 20-21 0.18091224904470238 1106 22-23 0.02556446638710952 1106 24-25 -0.05438396481495289 1106 26-27 0.11954461714800146 1106 28-29 0.24015494184344988 1106 30-31 0.2857112929509711 1106 32-33 0.35968119751584027 1106 34-35 0.3431057419960837 1106 36-37 8.626367654400724E-4 1106 38-39 -0.006518935814362692 1106 40-41 0.2952702992416363 1106 42-43 0.15246016844240273 1106 44-45 -0.020678118758105768 1106 46-47 -0.12056459178769785 1106 48-49 0.08295436148764423 1106 50-51 0.17099291231114222 1106 52-53 0.13855190182249544 1106 54-55 0.25250823346238604 1106 56-57 0.0031618602620824277 1106 58-59 0.07300628406706267 1106 60-61 0.183245323468185 1106 62-63 0.31471165497764275 1106 64-65 0.32782629756588477 1106 66-67 0.14785945767508935 1106 68-69 0.054714210162337906 1106 70-71 0.16174593390332603 1106 72-73 0.3710095870346386 1106 74-75 0.5245201946937392 1106 76-77 0.4112479069439239 1106 78-79 0.5077142204767249 1106 80-81 0.47201217223473435 1106 82-83 0.5216608041239539 1106 84-85 0.6426528057396723 1106 86-87 0.7277736404272552 1106 88-89 0.8016851870345185 1106 90-91 0.7148243053168635 1106 92-93 0.8570713415919435 1106 94-95 0.9085619699165548 1106 96-97 1.0254444131242515 1106 98-99 1.114085406277372 1106 100-101 1.2582303531382628 1107 1 0.06880695333617837 1107 2 0.03333536460035447 1107 3 0.02221682075826692 1107 4 0.04141803373818931 1107 5 0.09305923121523563 1107 6 0.20766032971710757 1107 7 0.08261592971359732 1107 8 0.17992453339352465 1107 9 0.17699287115656404 1107 10-11 0.13366203760799067 1107 12-13 0.22218752295733424 1107 14-15 0.23685590368489784 1107 16-17 0.17225611475110014 1107 18-19 0.33357268230856363 1107 20-21 0.33400233990532513 1107 22-23 0.3039092969004358 1107 24-25 0.18931691601029854 1107 26-27 0.2527917028749229 1107 28-29 0.23183382165134958 1107 30-31 0.22894349783077672 1107 32-33 0.15881242999595457 1107 34-35 0.24862715363156696 1107 36-37 0.257919463723816 1107 38-39 0.4355660162023085 1107 40-41 0.4809508279039889 1107 42-43 0.47366958774853885 1107 44-45 0.41541928583951915 1107 46-47 0.3630375378325894 1107 48-49 0.4722779002997086 1107 50-51 0.5312997638672883 1107 52-53 0.3644433539526659 1107 54-55 0.44427566524296225 1107 56-57 0.4697383136687563 1107 58-59 0.5700005330119282 1107 60-61 0.5130957947485939 1107 62-63 0.5857379253962023 1107 64-65 0.5375565323733014 1107 66-67 0.5495940988595365 1107 68-69 0.6099220564973393 1107 70-71 0.5301031694097702 1107 72-73 0.3989975087256248 1107 74-75 0.42300069557507314 1107 76-77 0.39835063687841554 1107 78-79 0.5562155571163423 1107 80-81 0.3761032154953021 1107 82-83 0.5314401058559568 1107 84-85 0.6670502568319101 1107 86-87 0.808596447093926 1107 88-89 0.83018773266258 1107 90-91 0.8670057256081876 1107 92-93 0.7952976590838858 1107 94-95 0.7511846324049287 1107 96-97 0.9119276329434278 1107 98-99 1.2716477897595055 1107 100-101 1.4800078683969211 1108 1 -0.07372335432903654 1108 2 -0.054195434647837715 1108 3 -0.1813919186285382 1108 4 -0.18968867975164017 1108 5 -0.1427620231894977 1108 6 0.07852271414375878 1108 7 -0.05802144116371721 1108 8 0.05317609324519168 1108 9 0.09656003713826067 1108 10-11 0.0012172599034485643 1108 12-13 0.07885411385540664 1108 14-15 0.08420049294473131 1108 16-17 0.06329394089723195 1108 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-1.3204036584836274 2316 68-69 -1.0458115158706747 2316 70-71 -1.0380131797633645 2316 72-73 -1.3763094399946745 2316 74-75 -1.4527537263331887 2316 76-77 -1.170455747422853 2316 78-79 -1.3134335131709811 2316 80-81 -1.2195170839284017 2316 82-83 -1.2495135933501693 2316 84-85 -1.7743325988246212 2316 86-87 -1.8645503144857116 2316 88-89 -1.6331700878436344 2316 90-91 -1.8516729433118293 2316 92-93 -1.8498240844128375 2316 94-95 -2.078949985034342 2316 96-97 -2.233860934012508 2316 98-99 -2.5773893185307806 2316 100-101 -2.705596391080867 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7199.0 3 1393.0 4 1368.0 5 1562.0 6 1710.0 7 2028.0 8 2552.0 9 3174.0 10 3972.0 11 4908.0 12 6559.0 13 8048.0 14 9430.0 15 11245.0 16 13781.0 17 17341.0 18 14965.0 19 11336.0 20 11688.0 21 11799.0 22 13141.0 23 14623.0 24 16407.0 25 18714.0 26 21968.0 27 25760.0 28 30782.0 29 36712.0 30 45431.0 31 55893.0 32 70673.0 33 91823.0 34 123775.0 35 172392.0 36 248953.0 37 342293.0 38 273696.0 39 34844.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85624318215045 19.409756975292673 15.16610249547351 22.567897347083367 2 14.827876961820389 20.18269101047803 41.1209358276746 23.868496200026982 3 18.953964358270103 24.964501052882472 29.684596211421947 26.39693837742548 4 12.292550042919228 16.719217189276883 37.996304505591304 32.99192826221259 5 13.48156091989685 37.72721447697672 34.28254745508544 14.508677148040979 6 31.33258028223127 36.69955788673116 17.789062934439055 14.178798896598519 7 28.263302175085386 32.88316780514111 20.783805950026967 18.06972406974654 8 26.081622473031057 35.37756516698161 19.70693114690742 18.83388121307991 9 26.247629554401165 17.14079323423148 20.3762829615516 36.23529424981576 10-11 24.56900716598617 27.18529995348513 27.460063502906618 20.78562937762208 12-13 25.635622558579353 24.365767824073618 28.122918814854682 21.875690802492354 14-15 22.468733825601404 25.698797207479114 26.556077192830706 25.27639177408878 16-17 21.707862505027535 29.314553004275933 26.94377473042294 22.033809760273588 18-19 21.668691015961233 27.67487218515986 29.561266170806405 21.095170628072506 20-21 22.462096265725645 26.69271049087741 29.64974239362591 21.19545084977104 22-23 22.061984684973627 26.706033612624086 29.541021027970455 21.690960674431835 24-25 21.871589786447267 26.910379517629067 29.642678420688156 21.57535227523551 26-27 21.749431624297692 27.183735256987262 29.43892646854379 21.62790665017125 28-29 21.524444154849117 27.212370988090516 29.623982312715768 21.639202544344595 30-31 21.66634506085091 26.93736607364801 29.593745848265534 21.802543017235543 32-33 21.525772975803058 27.085232804206054 29.48240015594621 21.90659406404468 34-35 22.073824221624324 27.164566845100012 29.180901079025563 21.580707854250104 36-37 21.966083663815823 26.954424449329245 29.262199065746287 21.81729282110864 38-39 21.70833595427873 27.170788664935348 29.357050268235042 21.763825112550883 40-41 21.941338832870404 27.055762284307306 29.287506104278343 21.715392778543944 42-43 21.801246387262946 27.09679158621201 29.041744313133457 22.060217713391587 44-45 21.852950544058608 27.29642950830538 28.871758777372147 21.97886117026386 46-47 21.95098320049876 27.45715874433417 28.498913171685174 22.092944883481895 48-49 22.125278963472006 27.53156717788671 28.314687086259184 22.028466772382096 50-51 22.041625833756758 27.681328314571964 28.222836600106564 22.05420925156471 52-53 22.249391637619112 27.679245341319174 28.067001744679093 22.004361276382618 54-55 21.987334013841238 27.63447287509347 28.199543494174016 22.178649616891278 56-57 22.035552645219912 27.963548794890787 28.040516065408422 21.960382494480875 58-59 22.184386967801352 27.602087802248406 28.09528756415584 22.11823766579441 60-61 22.165773892006136 27.49605558985884 28.48351294123131 21.854657576903712 62-63 22.033084473457503 27.634397249549885 28.446570754340822 21.88594752265179 64-65 21.87778855371179 27.64431406001066 28.31373542230461 22.16416196397294 66-67 21.878078506478555 27.863453319030157 28.06233115603008 22.196137018461208 68-69 21.887505770274018 27.72769697834196 28.22236810922581 22.16242914215821 70-71 21.87500877510734 27.654673551966884 28.22074519615524 22.249572476770535 72-73 22.046478083719865 27.53065942620787 28.169881923155128 22.252980566917138 74-75 21.917263526434528 27.887798787915546 27.984734093427438 22.210203592222484 76-77 22.01634360092811 27.733261963242906 27.983852985102708 22.266541450726272 78-79 21.971776798000505 27.649105563200315 28.10938245949058 22.269735179308604 80-81 21.86268979252935 28.026382330386767 27.957756148487515 22.153171728596366 82-83 21.98597673498377 27.915772789069415 27.924394454387595 22.173856021559217 84-85 21.999602406675077 27.835688381177437 27.809491024386844 22.355218187760638 86-87 21.994099324204985 27.72447151830223 27.942622989947175 22.33880616754561 88-89 22.063751533636378 27.638816382339588 27.859935225714487 22.43749685830955 90-91 21.995415503998306 27.67689384249339 27.98954714693329 22.338143506575015 92-93 22.096482675108007 27.537878192181275 28.011636423274194 22.35400270943652 94-95 21.899586992583163 27.537207034184384 27.97048992222026 22.59271605101219 96-97 21.960055217094386 27.496338329379626 28.000772769750558 22.542833683775427 98-99 21.78416425746175 27.504981051644272 27.87434410887968 22.836510582014295 100-101 21.88034005648141 27.31239362003815 28.215124640392908 22.59214168308753 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2420.0 1 1369.0 2 331.0 3 357.0 4 312.5 5 292.0 6 498.5 7 1018.5 8 1712.0 9 2083.0 10 1956.5 11 1792.5 12 1863.5 13 1999.0 14 2131.0 15 2274.0 16 2403.5 17 2594.5 18 2885.0 19 3113.5 20 3307.5 21 3788.5 22 4518.5 23 5479.5 24 6727.0 25 8389.5 26 10715.0 27 13284.0 28 15641.5 29 18486.5 30 22127.0 31 26107.0 32 30255.0 33 33971.0 34 37945.0 35 41368.0 36 45014.0 37 49008.5 38 51444.5 39 53432.0 40 55589.0 41 58859.5 42 61802.5 43 64675.0 44 67464.0 45 68754.0 46 68183.5 47 68039.5 48 71313.0 49 70625.5 50 66557.5 51 63090.5 52 59427.0 53 57332.5 54 54891.0 55 50844.5 56 45653.0 57 40075.5 58 35538.0 59 31156.5 60 25932.0 61 20818.0 62 16208.5 63 12191.5 64 9170.0 65 7124.0 66 5300.0 67 4011.0 68 2912.0 69 1895.0 70 1383.0 71 1034.0 72 764.5 73 616.5 74 464.5 75 362.5 76 253.5 77 139.5 78 79.5 79 53.0 80 36.0 81 25.5 82 18.0 83 14.0 84 11.0 85 10.0 86 8.5 87 6.0 88 5.5 89 2.5 90 0.5 91 2.5 92 3.0 93 3.5 94 3.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3094833293253533 2 0.27921326994558676 3 0.2811191625732017 4 0.28128732956975594 5 0.2682263595040419 6 0.2033699544996163 7 0.2119464713238835 8 0.20992846736523238 9 0.20656512743414723 10-11 0.21665514722740267 12-13 0.21612261840498087 14-15 0.19857719509781993 16-17 0.20992846736523238 18-19 0.19807269410815714 20-21 0.2022768690220136 22-23 0.20168828453407373 24-25 0.19305571204428848 26-27 0.20451909564273707 28-29 0.2062287934410387 30-31 0.2136561691221851 32-33 0.21525375558945054 34-35 0.20353812149617057 36-37 0.20121181137716998 38-39 0.1913179864132278 40-41 0.19213079356324006 42-43 0.1925512110546257 44-45 0.20796651907209937 46-47 0.19793255494436196 48-49 0.20684540576173766 50-51 0.21416067011184786 52-53 0.22184029628782567 54-55 0.2185049841894995 56-57 0.18576847552693726 58-59 0.17494973208194664 60-61 0.18254527475964732 62-63 0.18425497255794895 64-65 0.18072346563030953 66-67 0.19344810170291507 68-69 0.18290963658551487 70-71 0.18700170016833517 72-73 0.20039900422715776 74-75 0.18901970412698627 76-77 0.1989976125892056 78-79 0.19569032832363853 80-81 0.1838345550665633 82-83 0.19857719509781993 84-85 0.18089163262686378 86-87 0.18484355704588884 88-89 0.19342007387015603 90-91 0.18915984329078148 92-93 0.19602666231674704 94-95 0.19420485318740924 96-97 0.18683353317178092 98-99 0.19302768421152944 100-101 0.17814490501647756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1783941.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.302464398515454 #Duplication Level Percentage of deduplicated Percentage of total 1 78.10215516720646 46.316502762505905 2 12.746499406783055 15.117976545529007 3 4.039788593389326 7.187082577109978 4 1.7354405265290018 4.1166360016090815 5 0.9375338176347552 2.7799032921344673 6 0.5445024734978434 1.937420312970568 7 0.35274443995249555 1.4643030214439996 8 0.256845110455599 1.2185238414980744 9 0.17838365864197292 0.9520731509303277 >10 0.9966350625990192 10.808898543478769 >50 0.07228505446917954 2.9413953010697695 >100 0.035862255412481454 3.76100968436158 >500 8.514214897893892E-4 0.3229766079107893 >1k 2.838071632631296E-4 0.41027061553788463 >5k 1.8920477550875308E-4 0.6650277419097613 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6810 0.3817390821781662 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5044 0.2827447768732262 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3483 0.19524188299949383 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2560 0.14350250372630036 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 5.605566551808608E-5 0.0 0.0 0.0 0.0 12-13 5.605566551808608E-5 0.0 0.0 0.0 0.0 14-15 5.605566551808608E-5 0.0 0.0 0.0 0.0 16-17 8.408349827712913E-5 0.0 0.0 0.0 0.0 18-19 1.1211133103617216E-4 0.0 0.0 0.0 0.0 20-21 1.1211133103617216E-4 0.0 0.0 5.605566551808608E-5 5.605566551808608E-5 22-23 1.1211133103617216E-4 0.0 0.0 2.2422266207234432E-4 5.605566551808608E-5 24-25 1.401391637952152E-4 0.0 0.0 2.802783275904304E-4 5.605566551808608E-5 26-27 1.6816699655425825E-4 0.0 0.0 4.764731569037317E-4 8.408349827712913E-5 28-29 1.9619482931330129E-4 0.0 0.0 9.809741465665065E-4 1.1211133103617216E-4 30-31 2.2422266207234432E-4 0.0 0.0 0.0023823657845186584 1.1211133103617216E-4 32-33 2.2422266207234432E-4 0.0 0.0 0.003979952251784111 1.1211133103617216E-4 34-35 2.2422266207234432E-4 0.0 0.0 0.006222178872507554 1.1211133103617216E-4 36-37 2.2422266207234432E-4 0.0 0.0 0.009669602301869848 1.1211133103617216E-4 38-39 2.2422266207234432E-4 0.0 0.0 0.015275168853678456 1.1211133103617216E-4 40-41 2.2422266207234432E-4 0.0 0.0 0.02104890240204132 1.1211133103617216E-4 42-43 2.2422266207234432E-4 0.0 0.0 0.02665446895384993 1.1211133103617216E-4 44-45 2.2422266207234432E-4 0.0 0.0 0.03136314485736916 1.1211133103617216E-4 46-47 3.363339931085165E-4 0.0 0.0 0.03884657620403365 1.1211133103617216E-4 48-49 3.363339931085165E-4 0.0 0.0 0.045713395229999204 1.401391637952152E-4 50-51 3.363339931085165E-4 0.0 0.0 0.052636269921482834 1.6816699655425825E-4 52-53 3.363339931085165E-4 0.0 0.0 0.06802355010619746 1.6816699655425825E-4 54-55 3.363339931085165E-4 0.0 0.0 0.08932470300307016 1.6816699655425825E-4 56-57 3.363339931085165E-4 0.0 0.0 0.12166882200700585 1.6816699655425825E-4 58-59 3.363339931085165E-4 0.0 0.0 0.16668152141802894 1.6816699655425825E-4 60-61 3.363339931085165E-4 0.0 0.0 0.22242888077576556 1.6816699655425825E-4 62-63 3.363339931085165E-4 0.0 0.0 0.2645547134126073 1.6816699655425825E-4 64-65 3.363339931085165E-4 0.0 0.0 0.31990968311171725 1.6816699655425825E-4 66-67 3.363339931085165E-4 0.0 0.0 0.404413598880232 1.6816699655425825E-4 68-69 3.363339931085165E-4 0.0 0.0 0.5087612202421492 1.6816699655425825E-4 70-71 3.363339931085165E-4 0.0 0.0 0.6251608096904551 1.6816699655425825E-4 72-73 3.6436182586755956E-4 0.0 0.0 0.7454562678922678 1.6816699655425825E-4 74-75 3.9238965862660257E-4 0.0 0.0 0.873935853259721 1.6816699655425825E-4 76-77 3.9238965862660257E-4 0.0 0.0 1.0099829534721159 1.6816699655425825E-4 78-79 3.9238965862660257E-4 0.0 0.0 1.1470670834965955 1.6816699655425825E-4 80-81 3.9238965862660257E-4 0.0 0.0 1.2908218377177272 1.6816699655425825E-4 82-83 3.9238965862660257E-4 0.0 0.0 1.4718536095083863 1.6816699655425825E-4 84-85 3.9238965862660257E-4 0.0 0.0 1.7066147366981306 1.6816699655425825E-4 86-87 3.9238965862660257E-4 0.0 0.0 1.9866127859609706 1.6816699655425825E-4 88-89 3.9238965862660257E-4 0.0 0.0 2.309577502843424 1.6816699655425825E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8300 0.0 35.63639 1 GGTATCA 5105 0.0 34.37257 1 ATCAACG 9965 0.0 29.431068 3 TCAACGC 10035 0.0 29.179224 4 CAACGCA 10095 0.0 29.002535 5 TATCAAC 10150 0.0 28.943098 2 AACGCAG 10415 0.0 28.09642 6 GTACATG 7740 0.0 26.049913 1 TACATGG 8015 0.0 24.850384 2 ACGCAGA 11785 0.0 24.709301 7 CGCAGAG 11925 0.0 24.419212 8 ACATGGG 7930 0.0 23.856573 3 GAGTACT 6080 0.0 23.046518 12-13 GCAGAGT 12450 0.0 22.777075 9 AGTACTT 6455 0.0 21.192545 12-13 AGAGTAC 10510 0.0 21.038265 10-11 CGCGGAA 125 9.516953E-9 20.897915 82-83 GTACTTT 6765 0.0 20.4663 14-15 CATGGGA 4580 0.0 19.512045 4 CATGGGG 3560 0.0 18.693405 4 >>END_MODULE