FastQCFastQC Report
Thu 26 May 2016
SRR937281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783941
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86860.4868995106900957No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70650.3960332768852781No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51480.28857456608710713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55750.038.5411871
GTATCAA90750.036.1438141
ATCAACG112800.028.7227953
TATCAAC115450.028.3103922
TCAACGC114800.028.263784
CAACGCA116550.027.961685
AACGCAG120150.027.1185536
ACGCAGA135400.023.9596467
CGCAGAG136000.023.8539418
GAGTACT81150.022.35904312-13
GCAGAGT145800.021.989979
GTACATG72400.021.4704271
AGTACTT84600.021.2226612-13
GTACTTT85150.021.02920714-15
AGAGTAC126100.020.34028410-11
TACATGG76500.020.1203252
ACATGGG74900.019.5987363
TGCACCG3404.1836756E-1119.5614285
CGCGGAA2450.019.38707582-83
TCGCGGA2750.018.99933282-83
Produced by FastQC (version 0.11.5)